• 한국수산과학회지
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• 제37권4호
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• pp.269-274
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• 2004

Genetic responses of the edible seaweed Porphyra yezoensis tissue to acid shock have been compared using differential display technique. The tissue was challenged in seawater containing $0.05{\%}$ hydrogen chloride (pH 3.0) for 5 min, then rehabilitated in normal seawater for 10 min, 30 min, 60 min and 4 hrs. Total RNA extracted by the LiCl-guanidium method was reverse transcribed and amplified by PCR with arbitrary primers. The amplified fragment responded by the acid shock was selectively isolated from agarose gel and sequenced with DNA auto sequencer. Sequence (1056 bp) of the cDNA contained at least two genes for ASP7K (MW 7418) and ASP5K (MW 5512) proteins.

• 보존과학연구
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• 통권23호
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• pp.59-75
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• 2002

In this study human bones and teeth, excavated from the Myeongam-risite in Asan, Chungcheongnam-do Province, have been analysed by nuclear DNA typing and mitochondrial DNA sequencing methods. Twenty-one samples of long bones and twenty-seven samples of teeth from twenty-one individuals were collected and analysed. Among these thirteenteeth were successfully subjected to nuclear DNA extraction, quantification, and PCR(Polymerase Chain Reaction) amplification. Silver STR III (D16S539, D7S820, D13S317) multiplex PCR method was used in this study for a short tandem repeat (STR) analysis. Mitochondrial DNAs of tooth samples were also amplified and sequenced by a DNA sequencer. These analyses show that a sample from Burial no. 29 and one from Burial no. 38(right) possessed the same maternal inheritance. This may suggest that the Myeongam-ri cemetery was used by a kin group for a relatively long period of time.

• 한국산학기술학회논문지
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• 제5권5호
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• pp.429-432
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• 2004

본 논문은 단일형태의 버퍼 구조로 물리적인 단일 큐의 시퀀서 갖고 논리적으로 다중 큐의 형태로 구성된 ATM스위치를 제안한다. 제안된 스위치는 순환적 순위 알고리즘이 적용되도록 하드웨어로 구현된 ATM 셀의 출력 포트에 따라 ATM 셀이 정렬되도록 프로시져를 수행시킨다. 이 구조는 물리적으로 단일 버퍼를 갖지만 논리적으로는 순환적으로 순위를 결정하여 출력포트에서의 상충을 최대한 억제하도록 설계된 다중 큐의 기능을 하고 있다. 향후 이러한 형태의 구조는 다양한 구조의 라우팅을 위한 스위치로 응용되고, 시스템 구성의 확장성에 있어서도 큰 장점을 지니고 있어, 전송 효율적인 면에서도 훌륭한 구조로 평가된다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권9호
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• pp.1234-1240
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• 2009

Gene flow, genetic structure and differentiation of Kutchi, Mehsana and Sirohi breeds of goat from North-Western India were evaluated based on 25 microsatellite markers so as to support breed conservation and improvement decisions. The microsatellite genotyping was carried out using an automated DNA sequencer. The gene diversity across the studied loci for the Kutchi breed varied from 0.57 (ILST 065) to 0.93 (OarFCB 304, OMHC 1, ILSTS 058) with an overall mean of 0.79${\pm}$0.02. The corresponding values for Mehsana and Sirohi breeds were 0.16 (ILST 008) to 0.93 (OMHC 1, ILSTS 058) with an average of 0.76${\pm}$0.04, and 0.50 (ILSTS 029) to 0.94 (ILSTS 058) with an average of 0.78${\pm}$0.02, respectively. The Mehsana breed had lowest gene diversity among the 3 breeds studied. All the populations showed an overall significant heterozygote deficit ($F_{is}$). The Fis values were 0.26, 0.14 and 0.36 for Kutchi, Mehsana and Sirohi goat breeds, respectively. Kutchi and Mehsana were more differentiated (16%) followed by Mehsana and Sirohi (13%).The measures of standard genetic distance between pairs of breeds indicated that the lowest genetic distance was between Kutchi and Sirohi breeds (0.73) and the largest genetic distance was between Mehsana and Kutchi (1.0) followed by Sirohi and Mehsana (0.75) breeds. Mehsana and Kutchi are distinct breeds and this was revealed by the estimated genetic distance between them. All measures of genetic variation revealed substantial genetic variation in each of the populations studied, thereby showing good scope for their further improvement.

• 대한화학회지
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• 제48권1호
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• pp.33-38
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• 2004

두경부 편평 세포암종(HNSCC: head and neck squamous cell carcinoma) 의 발생과 관련하여 p53 종양 억제 유전자 (tumor suppressor gene) 의 돌연변이는 높은 비율로 나타나는 것으로 보고 되고 있다. 단국대학교 병원에서 두경부 종양으로 진단 받고 수술 받은 환자의 조직 50개를 대상으로 p53 종양 억제 유전자의 exon 5-8 까지의 영역에서 DNA를 추출하여 PCR-SSCP(polymerase chain reaction single strand conformational polymorphism) 방법과 DHPLC(denaturing high performance liquid chromatography) 방법으로 p53체세포 돌연변이(somatic mutation)를 비교 분석하였다. 그 결과 SSCP 분석 방법은 16개(32%), DHPLC 분석 방법은 17개(34%) 를 검출하였고 그 중 SSCP와 DHPLC 분석 방법 모두 exon 8번에서 결실(deletion) 형태의 돌연변이를 확인하였으며 최종적으로 자동 염기 서열 분석기(automatic DNA sequencer) 를 통하여 모든 돌연변이를 확인하였다. DHPLC 분석방법이 SSCP 방법보다 분석 시간이나 노력이 덜 소모되며 보다 더 정확한 돌연변이 검출 방법임을 확인하였다.

• KSBB Journal
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• 제14권6호
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• pp.702-706
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• 1999

대표적인 양식 해조류인 방사무늬 김 엽체를 대상으로 하여 산 처리에 의한 유전형질의 표현 변화를 differential display기법으로 비교하여 보았다. 방사무늬 김 엽체를 0.05% HCl을 첨가한 해수(pH 3.0)에서 5분간 처리한 후 각각 10분, 30분, 60분 그리고 4시간동안 멸균 해수에서 정치 배양시키면서, RNA를 추출하여 cDNA합성, PCR 증폭, agarose gel 전기영동 및 DNA 염기배열을 조사하였다. 그 결과 arbitrary primer OPA 1(CAGGCCCTTC)을 사용하여 differential display한 경우, 산 처리 후 30분간 멸균 해수에서 정치 배양한 엽체에서 특이적으로 RNA 합성이 일어나지 않은 유전자를 분리할 수 있었으며, 그 염기서열을 비교한바 이 유전자 fragment(605 bp)는 dethiobiotin synthetase 유전자와 93%의 높은 상동성을 가진 것으로 나타났다.

• 인터넷정보학회논문지
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• 제3권2호
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• pp.79-85
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• 2002

개방형 통신망 구조에서 어플리케이션 시스템은 다수의 컴퓨팅 노드에 분산되어 있는 객체들의 집합으로 구성된다. 이러한 분산된 환경에서 복잡한 분산 소프트웨어의 개발 및 관리의 복잡성을 줄이고 분산된 객체들을 효율적으로 관리하면서 객체들의 실시간 서비스를 제공하기 위해서는 객체 그룹 관리 및 통신 기법이 필요하다. 본 연구에서는 CORBA를 기반으로 하여 기존의 시스템에 큰 변환 얼이 새로운 서비스를 적용시킬 수 있도록 객체그룹을 구성하고 신뢰성 있는 그룹통신 기법을 제공한다. 그룹관리의 효율향상을 위해 그룹을 서브그룹으로 나누고 서브그룹 관리자를 두어 서브그룹을 관리하도록 하였으며, 시퀀서를 두어 메시지의 순서성을 유지시킨다. 그룹간의 통신방법은 IP 멀티캐스트와 일대일 통신을 기반으로 한 멀티캐스트 기법을 혼용하여 사용하며 메시지 저장소를 두어 오류 발생 시 선택적으로 메시지를 재 전송할 수 있도록 함으로서 성능을 향상시켰다. 제안된 기법을 분산환경에서 시뮬레이션을 통하여 성능을 평가하였다.

• Genomics & Informatics
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• 제17권3호
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• pp.32.1-32.6
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• 2019

Currently, Illumina sequencers are the globally leading sequencing platform in the next-generation sequencing market. Recently, MGI Tech launched a series of new sequencers, including the MGISEQ-2000, which promise to deliver high-quality sequencing data faster and at lower prices than Illumina's sequencers. In this study, we compared the performance of two major sequencers (MGISEQ-2000 and HiSeq 4000) to test whether the MGISEQ-2000 sequencer delivers high-quality sequence data as suggested. We performed RNA sequencing of four human colon cancer samples with the two platforms, and compared the sequencing quality and expression values. The data produced from the MGISEQ-2000 and HiSeq 4000 showed high concordance, with Pearson correlation coefficients ranging from 0.98 to 0.99. Various quality control (QC) analyses showed that the MGISEQ-2000 data fulfilled the required QC measures. Our study suggests that the performance of the MGISEQ-2000 is comparable to that of the HiSeq 4000 and that the MGISEQ-2000 can be a useful platform for sequencing.

• Animal cells and systems
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• 제5권3호
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• pp.237-241
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• 2001

Three STR loci (STRX1[AGAT]$_{n}$, HPRTB[AGAT]$_{n}$ and ARA[AGC]$_{n}$) on X chromosome and three other STR loci (DYS390[CTG(A)T]$_{n}$, DYS392[ATT]$_{n}$ and DYS39［GATA]$_{n}$) on Y chromosome were analyzed in 154 unrelated healthy Korean subjects. Four loci (STRX1, HPRTB, DYS390 and DYS393) were amplified by quadruplex polymerase chain reaction (PCR) using fluorescent labeled primers (FLP). ARA and DYS392 were amplified separately using single PCR, similarly by using FLP. They were then run in an automated DNA sequencer and were analyzed with Genescan software. We found 7 alleles (308-332 bp) in STRX1, 7 alleles (275-299 bp) in HPRTB, 16 alleles (252-315 bp) in ARA, 6 alleles (203-223 bp) in DYS390, 7 alleles (245-263bp) in DYS392 and 5 alleles (116-132 bp) in DYS393. The ＊13(34%), ＊13(5l%), ＊23 (l8%), ＊23(50%), ＊14(39%) and ＊13(40%) alleles were observed to be the highest frequencies of STRX1, HPRTB, ARA, DYS390, DYS392 and DYS393, respectively. The detection of STR loci on sex chromosomes by quadruplex PCR allows simple determination of sex and identification of an individual. individual.

• Genomics & Informatics
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• 제8권3호
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• pp.131-137
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• 2010

Genome-wide association studies (GWASs) have greatly contributed to the identification of common variants responsible for numerous complex traits. There are, however, unavoidable limitations in detecting causal and/or rare variants for traits in this approach, which depends on an LD-based tagging SNP microarray chip. In an effort to detect potential casual and/or rare variants for complex traits, such as type 2 diabetes (T2D) and triglycerides (TGs), we conducted a targeted resequencing of loci identified by the Korea Association REsource (KARE) GWAS. The target regions for resequencing comprised whole exons, exon-intron boundaries, and regulatory regions of genes that appeared within 1 Mb of the GWA signal boundary. From 124 individuals selected in population-based cohorts, a total of 0.7 Mb target regions were captured by the NimbleGen sequence capture 385K array. Subsequent sequencing, carried out by the Roche 454 Genome Sequencer FLX, generated about 110,000 sequence reads per individual. Mapping of sequence reads to the human reference genome was performed using the SSAHA2 program. An average of 62.2% of total reads was mapped to targets with an average 22X-fold coverage. A total of 5,983 SNPs (average 846 SNPs per individual) were called and annotated by GATK software, with 96.5% accuracy that was estimated by comparison with Affymetrix 5.0 genotyped data in identical individuals. About 51% of total SNPs were singletons that can be considered possible rare variants in the population. Among SNPs that appeared in exons, which occupies about 20% of total SNPs, 304 nonsynonymous singletons were tested with Polyphen to predict the protein damage caused by mutation. In total, we were able to detect 9 and 6 potentially functional rare SNPs for T2D and triglycerides, respectively, evoking a further step of replication genotyping in independent populations to prove their bona fide relevance to traits.