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http://dx.doi.org/10.5012/jkcs.2004.48.1.033

Analysis of p53 Somatic Mutation in Head and Neck Cancer Using Denaturing High Performance Liquid Chromatography(DHPLC)  

Kim, Kwang-Youl (Department of Chemistry, College of Natural Science, Dankook University)
Park, Sang-Bum (Department of Chemistry, College of Natural Science, Dankook University)
Han, Sang-Man (Department of Chemistry, College of Natural Science, Dankook University)
Nam, Youn-Hyoung (Department of Chemistry, College of Natural Science, Dankook University)
Jang, Won-Cheoul (Department of Chemistry, College of Natural Science, Dankook University)
Publication Information
Journal of the Korean Chemical Society / v.48, no.1, 2004 , pp. 33-38 More about this Journal
Abstract
Mutation of p53 tumor suppressor gene in HNSCC (head and neck squamous cell carcinoma) has been proposed high rate. We extracted genomic DNA from 50 head and neck cancer. The DNA was amplified by PCR at exon 5-8 in p53 tumor suppressor gene. We have compared single strand conformation polymorphism (SSCP) and denaturing high performance liquid chromatography (DHPLC) method for analysis of p53 somatic mutation. As a result, 16 deleted mutations (32%) were detected by SSCP analysis and 17 deleted mutations (34%) were detected by DHPLC analysis at exon 8. All of 17 mutations were proved by sequencing. We conclude that DHPLC is a fast and simple screening method rather than SSCP analysis.
Keywords
HNSCC; p53; DHPLC; PCR-SSCP;
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