• Journal of Microbiology
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• 제44권6호
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• pp.694-698
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• 2006

Several marine bacterial strains were isolated from Undaria pinnatifida (Miyok in Korean). Sixty-six strains were isolated on R2A agar media at $10^{\circ}C$ and identified by a phylogenetic analysis of the 16S rRNA gene sequences. They were grouped into 10 different sequence types based on the initial sequence analysis of the 5' domain of the gene (approximately 500 bp). Full sequences of 16S rRNA gene, were obtained from one strain in each sequence type and the species-affiliation was determined using phylogenetic and sequence similarity analyses. The results of the analyses indicated that they were closely related to Psychrobacter aquimaris, P. celer, P. nivimaris, P. pulmonis, Psychromonas arctica or Bacillus psychrodurans. These bacteria are marine or psychrotrophic bacteria. Because the sporophytes of U. pinnatifida are cultured on the costal area during winter, the U. pinnatifida-associated bacteria appeared to grow at low temperatures. U. pinnatifida sporophytes can be a good source for the isolation of psychrotrophic bacteria.

• Genomics & Informatics
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• 제1권1호
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• pp.55-60
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• 2003

The nucleotide sequence of pZMO1, a small cryptic plasmid of Zymomonas mobilis ATCC10988 was determined. Analysis of 1,680 bp of sequence revealed $69\%$ identity with Shigella sonnei plasmid, pKYM and $61\%$ identity with Nostoc sp. ss DNA replicating plasmid. Analysis of a deduced amino acid sequence of an orf of pZMO1 revealed $75\%$ identity and $90\%$ similarity with the repA gene of Synechocystis sp. plasmid pCA2.4. The upstream region of the repA gene of pZMO1 possesses six directed repeat sequences and two inverted repeat sequences at downstream of the IR consensus sequence of nick region of rolling circle replication (RCR) plasmid. A typical terminator hairpin structure was found at the downstream region of repA gene. Degradation of single-stranded plasmid DNA by S1 nuclease was detected by Southern hybridization. It suggests that pZMO1 replicates by a rolling circle mechanism in Z. mobilis ATCC10988 cells.

• 대한전자공학회논문지
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• 제21권6호
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• pp.105-114
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• 1984

본 논문에서는 direct sequence(DS) 방식을 이용한 spread spectrum system에 사용할 수 있는 빠른 초기 동기 방법을 제안하였다. 수식적인 모델을 세우고 해석을 한 후 computer simulation을 거쳐 제안된 system의 성능을 분석하여 sliding correlator에 비해 우수함을 입증하였다. 입력 신호의 signal-to-noise ratio(SNR)가 -18dB에서 초기 동기 시간이 45ms 소요되었다. 이 방법의 hardsware 구현과 실험 결과도 설명하였다.

• 한국산업정보학회논문지
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• 제17권3호
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• pp.43-49
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• 2012

현재 운용되고 있는 리가시 시스템에 대한 요구명세서는 대부분 자료흐름도를 사용하고 있어, 시스템의 유지 보수를 위해서는 자료흐름도로부터 획득한 정보에 의존하지 않을 수 없다. 본 논문에서는 자료흐름도로부터 데이터베이스 테이블 설계순서를 추출하는 추출기를 설계, 구현하였다. 추출기는 자료흐름도를 입력 받아 저장하고, 이를 방향그래프로 변환하여 데이터베이스 테이블 설계순서를 추출하여 제시한다. 구현된 추출기는 실제 운용 중인 소프트웨어 시스템에 적용함으로써 실제 적용가능성을 보였다.

• 산업기술연구
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• 제19권
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• pp.217-226
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• 1999

A single phase to three phase power converter with cost effective and simple structure is proposed. The converter consists of rectifier and inverter. The rectifier is composed of a half wave rectifier, a dc link capacitor, and a current limiting inductor, and the inverter is of only two switches with PWM control. For negative sequence operation the inverter output voltage leads the line input by $60^{\circ}$, and for positive sequence operation the inverter output voltage leads by $60^{\circ}$. We can see that positive sequence operation shows higher output voltage, slight harmonic distortion(2%), and better performances such as high efficiency and high power factor. A mathematical model for system analysis is provided, and specifications for selection and control scheme both for start-up and for steady state are analyzed. comparison and operational limits of positive and negative sequence operation are performed, and simulations and experiments are executed to verify the proposed.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2000년도 추계학술대회논문집A
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• pp.693-698
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• 2000

Aspects of classical information theory, such as rate distortion theory, investigate how to encode and decode information from an independently identically distributed source so that the asymptotic distortion rate between the source and its quantized representation is minimized. However, in most natural dynamics, the source state is highly corrupted by disturbances, and the measurement contains the noise. In recent coder-estimator sequence is developed for state estimation problem based on observations transmitted with finite communication capacity constraints. Unlike classical estimation problems where the observation is a continuous process corrupted by additive noises, the condition is that the observations must be coded and transmitted over a digital communication channel with finite capacity. However, coder-estimator sequence does not provide such a quantitative analysis as a variance for estimation error. In this paper, under the assumption that the estimation error is Gaussian distribution, a variance for coder-estimation sequence is proposed and its fitness is evaluated through simulations with a simple example.

• Mycobiology
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• 제33권2호
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• pp.109-112
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• 2005

The internal transcribed spacer (ITS) regions including the 3'-end of 18S rRNA gene, 5.8S rRNA gene and the 5'-end of the 28S rRNA gene of Rhizopus spp. were amplified by PCR and analyzed by DNASIS program. Length polymorphism of these region ranged from 564 bp in R. oryzae to 789bp in R. stolonifer. The length and sequence of 5.8S was very conserved with $154{\sim}155\;bp$. The sequence of ITS2 was more variable than that of ITS1. The base substitution rates were ranged from 0 to 0.6069 per site, and higher rate was found in R. stolonifer. In general, transition was usually more frequent than transversion. On the basis of sequencing results, four groups were clustered with value of 61.9% similarity; R. oryzae, R. micros pores, R. homothallicus, and R. stolonifer groups.

• 한국식품영양학회지
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• 제4권2호
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• pp.161-166
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• 1991

The blocked N-terminus and N-terminal sequence of soybean B-amylase were aetermined by analyzing the acidic peptides derived on peptic digestion of the enzyme. The acidic peptides were separated from the digest on a Dowex 50$\times$2 column(1X5cm) and purified by reversed phase-high performance liquid chromatography(RP-HPLC). The major acidic peptide, PEP-1, was a heptapeptlde. The N-terminal 7 amino acid sequence of soybean B-amylase was deduced to be acetyl-Ala-Thf-Ser-Asp-Ser-Asn-Met- from the results of sequence analysis of PEP-1 and amino acid analysis of other acidic peptides.

• BMB Reports
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• 제43권6호
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• pp.407-412
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• 2010

Homeodomain (HD) is a highly conserved DNA-binding domain composed of helix-turn-helix motif. Drosophila Vnd (Ventral nervous system defective) containing HD acts as a regulator to either enhance or suppress gene expression upon binding to its target sequence. In this study, kinetic analysis of Vnd binding to DNA was performed. The result demonstrates that DNA-binding affinity of the recombinant protein containing HD and NK2-specific domain (NK2-SD) was higher than that of the full-length Vnd. To access whether phosphorylation sites within HD and NK2-SD affect the interaction of the protein with the target sequence, alanine substitutions were introduced. The result shows that S631A mutation within NK2-SD does not contribute significantly to the DNA-binding affinity. However, S571A and T600A mutations within HD showed lower affinity for DNA binding. In addition, DNA-binding analysis using embryonic nuclear protein also demonstrates that Vnd interacts with other nuclear proteins, suggesting the existence of Vnd as a complex.

• 한국축산식품학회지
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• 제35권1호
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• pp.86-90
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• 2015

In this study, we reported the morphogenetic analysis and genome sequence by genomic analysis of the newly isolated staphylococcal phage SMSAP5 from soil of slaughterhouses for cattle. Based on transmission electron microscopy evident morphology, phage SMSAP5 belonged to the Siphoviridae family. Phage SMSAP5 had a double-stranded DNA genome with a length of 45,552 bp and 33 % G+C content. Bioinformatics analysis of the phage genome revealed 43 open reading frames. A blastn search revealed that its nucleotide sequence shared a high degree of similarity with that of the Staphylococcus phage tp310-2. In conclusion, this study is the first report to show the morphological features and the complete genome sequence of the phage SMSAP5 from soil of slaughterhouses for cattle.