Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Sequence Analysis of the Internal Transcribed Spacer of Ribosomal DNA in the Genus Rhizopus

  • Published : 2005.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The internal transcribed spacer (ITS) regions including the 3'-end of 18S rRNA gene, 5.8S rRNA gene and the 5'-end of the 28S rRNA gene of Rhizopus spp. were amplified by PCR and analyzed by DNASIS program. Length polymorphism of these region ranged from 564 bp in R. oryzae to 789bp in R. stolonifer. The length and sequence of 5.8S was very conserved with $154{\sim}155\;bp$. The sequence of ITS2 was more variable than that of ITS1. The base substitution rates were ranged from 0 to 0.6069 per site, and higher rate was found in R. stolonifer. In general, transition was usually more frequent than transversion. On the basis of sequencing results, four groups were clustered with value of 61.9% similarity; R. oryzae, R. micros pores, R. homothallicus, and R. stolonifer groups.

Keywords

References

  1. Ellis, J. J. 1985. Species and varieties in the Rhizopus arrhizus - Rhizopus oryzae group as indicated by their DNA complementarity. Mycologia. 77: 243-247 https://doi.org/10.2307/3793074
  2. Inui, T., Takeda, Y. and Iizuka, H. 1965. Taxonomical studies on genus Rhizopus. J Gen. Appl. Microbiol. Suppl. 11: 1-12l
  3. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol. Evol. 16: 111-120 https://doi.org/10.1007/BF01731581
  4. Lee, Y.-M., Choi, Y. K. and Min, B. R. 2000b. Molecular characterization of Fusarium solani and its formae speciales based on sequence analysis of the internal transcribed spacer (ITS) region of ribosomal RNA. Mycobiology 28: 82-88
  5. Lee, Y.-M., Choi, Y. K. and Min, B. R. 2000a. PCR-RFLP and sequence analysis of the rDNA ITS region in the Fusarium spp, J Micrbiol. 38: 66-73
  6. Maicas, S., Adam, A. C. and Polaina, J. 2000. The ribosomal DNA of Zygomycete Mucor miehei. Curr. Genet. 37: 412-419 https://doi.org/10.1007/s002940000122
  7. Park, Y. J., Choi, Y. K. and Min, B. R. 2003. PCR-DGGE and PCR-RFLP analysis of the internal spacer (ITS) of ribosomal DNA in the genus Rhizopus. J Microbiol. 41: 157-160
  8. Paul, B. 2002. ITS region of the rDNA of Pythium longandrum, a new species : its taxonomy and its compared with related species. FEMS Microbiol. Lett. 202: 239-242 https://doi.org/10.1111/j.1574-6968.2001.tb10810.x
  9. Rau, H. A. and Planta, R. J. 1995. The pathway to maturity: processing of ribosomal RNA in Saccharomyces cerevisiae. Gene Expression 5: 71-77
  10. Schipper, M. A A. 1984. The R. stolonifer-group and R. oryzae. A revision of the genus Rhzopus. Studies in Mycology 25
  11. Schipper, M. A A. and Stalpers, J. A. 1984. The R. microsoporus-group. A revision of the genus Rhizopus. Studies in Mycology 25
  12. Scholtterer, C., Hauser, M.-T., Haeseler, A. and Tautz, D. 1994. Comparative evolutionary analysis of rDNA ITS region in Drosophila Mol. Biol. Evol. 11: 513-522
  13. Su, Y.-C., Huang, H., Liu, X.-Y. and Zheng, R.-Y. 1999. Systematic relationship of several controversial Cunninghamella taxa inferred from sequence comparisons of ITS2 of rDNA. Mycol. Res. 103: 805-810 https://doi.org/10.1017/S0953756298007667
  14. Sutton, D. A., Fothergill, A. W. and Rinaldi, M.G. 1998. Guide to Clinically significant fungi. The Williams & Silkins Co., Baltimore, Md
  15. Takamatsu, S., Hirata, T. and Sato, Y. 1998. Phylogenetic analysis and predicted secondary structure of the rDNA internal transcribed spacer of the powdery mildew fungi (Erysiphaceae). Mycoscience 39: 441-453 https://doi.org/10.1007/BF02460905
  16. van der Sande, C. A. F. M., Kwa, R. W. van Nues, H. van Heerikhuizen, H. A. and Plamta, R. J. 1992. Functional analysis of internal transcribed spacer 2 of Sacchromyces cerevisiae ribosomal DNA. J Mol. Biol. 223: 899-910 https://doi.org/10.1016/0022-2836(92)90251-E
  17. White, T. J., Bruns, T. D., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal genes form phylogenetics. Pp 315-322. In: Innis, M. A., Gelfrand, D. H. Sninsky, J. J. and White, T. J. Eds. PCR protocols. Academic Press, San Diego, California, USA

Cited by

  1. in a Patient with Diabetes vol.34, pp.1, 2014, https://doi.org/10.3343/alm.2014.34.1.76
  2. Rhizopus homothallicus Causing Invasive Infections: Series of Three Cases from a Single Centre in North India vol.182, pp.9-10, 2017, https://doi.org/10.1007/s11046-017-0153-5