• Korean Chemical Engineering Research
• /
• v.43 no.3
• /
• pp.331-343
• /
• 2005

Development of hydrogen station system is an important technology to commercialize fuel cells and fuel cell powered vehicles. Generally, hydrogen station consists of hydrogen production process including desulfurizer, reformer, water gas shift (WGS) reactor and pressure swing adsorption (PSA) apparatus, and post-treatment process including compressor, storage and distributer. In this review, we investigate the R&D trends and prospects of hydrogen station in domestic and foreign countries for opening the hydrogen economy society. Indeed, the reforming of fossil fuels for hydrogen production will be essential technology until the ultimate process that may be water hydrolysis using renewable energy source such as solar energy, wind force etc, will be commercialized in the future. Hence, we also review the research trends on unit technologies such as the desulfurization, reforming reaction of fossil fuels, water gas shift reaction and hydrogen separation for hydrogen station applications.

• Journal of Microbiology and Biotechnology
• /
• v.22 no.2
• /
• pp.199-206
• /
• 2012

Naturally immobilized tannase (tannin acyl hydrolase, E.C. 3.1.1.20) has many advantages, as it avoids the expensive and laborious operation of isolation, purification, and immobilization, plus it is highly stable in adverse pH and temperature. However, in the case of cell-associated enzymes, since the enzyme is associated with the biomass, separation of the pure biomass is necessary. However, tannic acid, a known inducer of tannase, forms insoluble complexes with media proteins, making it difficult to separate pure biomass. Therefore, this study optimizes the production of cell-associated tannase using a "protein-tannin complex" free media. An exploratory study was first conducted in shake-flasks to select the inducer, carbon source, and nitrogen sources. As a result it was found that gallic acid induces tannase synthesis, a tryptose broth gives higher biomass, and lactose supplementation is beneficial. The medium was then optimized using response surface methodology based on the full factorial central composite design in a 3 l bioreactor. A $2^3$ factorial design augmented by 7 axial points (${\alpha}$ = 1.682) and 2 replicates at the center point was implemented in 17 experiments. A mathematical model was also developed to show the effect of each medium component and their interactions on the production of cell-associated tannase. The validity of the proposed model was verified, and the optimized medium was shown to produce maximum cell-associated tannase activity of 9.65 U/l, which is 93.8% higher than the activity in the basal medium, after 12 h at pH 5.0, $30^{\circ}C$. The optimum medium consists of 38 g/l lactose, 50 g/l tryptose, and 2.8 g/l gallic acid.

• Journal of Broadcast Engineering
• /
• v.24 no.6
• /
• pp.1143-1151
• /
• 2019

In this paper, we propose a wireless transmission scheme that maximizes transmission efficiency per frequency bandwidth in a single carrier modulation scheme. The proposed scheme employs a decision directed channel tracking technique to remove both pilot signal and the guard interval signal between symbols in frames. It performs a raised cosine pulse shaping function with an roll-off factor of 0.05. In addition, 2×2 multiple input and multiple output using two polarized antennas is applied and both equalization and signal separation are performed in the frequency domain. The wireless modem with this technology confirms that the transmission speed of up to 63.3Mbps is achieved under the 5MHz frequency bandwidth

• Smart Media Journal
• /
• v.7 no.2
• /
• pp.54-59
• /
• 2018

With the increasing use of touch-enabled mobile devices such as smartphones and tablet PCs, the works are done on desktop computers and smartphones, and tablet PCs perform laptops. However, due to the nature of smart devices that require portability, QWERTY keyboard is densely arranged in a small screen. This is the cause of different typographical errors when using the mechanical QWERTY keyboard. Unlike the mechanical QWERTY keyboard, which has enough space for each button, QWERTY keyboard on the touch screen often has a small area assigned to each button, so that it is often the case that the surrounding buttons are input rather than the button the user intends to press. In this paper, we propose a method to automatically correct the input errors of the QWERTY keyboard in the touch screen environment by using the n-gram language model using the word unigram and the bigram probability.

• Nuclear Engineering and Technology
• /
• v.51 no.7
• /
• pp.1799-1804
• /
• 2019

The concept of a new method, the CARBEX (CARBonate EXtraction) process, was proposed for reprocessing of spent uranium oxide fuel. The proposed process is based on use of water solutions of $Na_2CO_3$ or $(NH_4)_2CO_3$ and solvent extraction (SE) by the quaternary ammonium compounds for selective recovery and purification of U from the fission products (FPs). Applying of SE allows to reach high degree of purification of U from FPs. Carrying out the processes in poorly aggressive alkaline carbonate media leads to increasing safety of SNF's reprocessing and better selectivity of separation of lanthanides and actinides. Moreover carbonate reprocessing media allows to carry out a recycling and regeneration of reagents. We have been done laboratory scale experiments on the extraction components of simulated voloxidated spent fuel in the solutions of NaOH or $Na_2CO_3-H_2O_2$ and recovery of U from carbonate solutions by SE method using carbonate of methyltrioctylammonium in toluene. It was shown that the purification factors of U from impurities of simulated FPs reached values $10^3-10^5$. The received results support our opinion that CARBEX after the further development can become more safe, simple and profitable method of spent fuel reprocessing.

• Smart Media Journal
• /
• v.11 no.8
• /
• pp.21-28
• /
• 2022

Currently, as we enter the 21st century, the level and interest of society, culture, economy, and science are rapidly developing, but science education is still struggling. In order to increase the efficiency of science education, it is most important to focus on elementary education based on basic science. Therefore, this study aims to analyze the main causes of Korea's science museum's regression as a museum focusing on experience, interest, and fun such as simple science experience centers and science theme parks. To this end, the influencing factors were identified by applying the algorithm of the actor-based model based on the data on the exhibition space and the exhibition movement of the science museum completed and operated in Korea over the past 5 years, and the problem of the visitor movement in the exhibition space was analyzed through the space system. In this study, it was confirmed that the exhibition environment was the best when the linear plot movement system and the picalesque plot were applied simultaneously in the museum's exhibition narrative theory, and the arrangement of major exhibition spaces, width of exhibition spaces, and separation of spaces for exhibition purposes were derived.

• Journal of Digital Convergence
• /
• v.20 no.2
• /
• pp.399-405
• /
• 2022

This study suggests focused in user-centered design which are new separate discharge system that must recycle in paper materials. I searched cases in Korea's current law and regulation in recycling disposal guidelines and already succeed cases in each city that are Asia, Europe, and North America. Suggesting focused in user-centered design of ID(Identification) separate discharge system due to user's good participations, feedbacks of participation's results, and well-organized in awarding. This system consists of visual individual's ID(Identification) that are stickered in only used bag. User must recycle in separate discharge system only used bags, and results must visualized in each user and rewards to user. This separate discharge system is meaning to stimulate user's good participations.

• Journal of Animal Reproduction and Biotechnology
• /
• v.39 no.1
• /
• pp.19-30
• /
• 2024

Background: Although an understanding of the proliferation and differentiation of fish female germline stem cells (GSCs) is very important, an appropriate threedimensional (3D) research model to study them is not well established. As a part of the development of stable 3D culture system for fish female GSCs, we conducted this study to establish a 3D aggregate culture system of ovarian cells in marine medaka, Oryzias dancena. Methods: Ovarian cells were separated by Percoll density gradient centrifugation and two different cell populations were cultured in suspension to form ovarian cell aggregates to find suitable cell populations for its formation. Ovarian cell aggregates formed from different cell populations were evaluated by histology and gene expression analyses. To evaluate the media supplements, ovarian cell aggregate culture was performed under different media conditions, and the morphology, viability, size, gene expression, histology, and E2 secretion of ovarian cell aggregates were analyzed. Results: Ovarian cell aggregates were able to be formed well under specific culture conditions that used ultra-low attachment 96 well plate, complete mESM2, and the cell populations from top to 50% layers after separation of ovarian cells. Moreover, they were able to maintain minimal ovarian function such as germ cell maintenance and E2 synthesis for a short period. Conclusions: We established basic conditions for the culture of O. dancena ovarian cell aggregates. Additional efforts will be required to further optimize the culture conditions so that the ovarian cell aggregates can retain the improved ovarian functions for a longer period of time.

• Journal of Microbiology
• /
• v.44 no.6
• /
• pp.600-606
• /
• 2006

Spore forming actinobacteria (sporoactinobacteria) isolated from soils with an acidic pH in Pinus thunbergii forests and coal mine waste were subjected to taxonomic characterization. For the isolation of acidophilic actinobacteria, acidified starch casein agar (pH adjusted to 4-5) was used. The numbers of actinobacteria growing in acidic media were between $3.2{\times}10^4$ and $8.0{\times}10^6$ CFU/g soil. Forty three acidophilic actinobacterial strains were isolated and their 16S rDNA sequences were determined. The isolates were divided into eight distinctive phylogenetic clusters within the variation encompassed by the family Streptomycetaceae. Four clusters among them were assigned to the genus Streptacidiphilus, whereas the remaining four were assigned to Streptomyces. The clusters belonging to either Streptomyces or Streptacidiphilus did not form a monophyletic clade. The growth pH profiles indicated that the representative isolates grew best between pH 5 and 6. It is evident from this study that acidity has played a critical role in the differentiation of the family Streptomycetaceae, and also that different mechanisms might have resulted in the evolution of two groups, Streptacidiphilus (strict acidophiles) and neutrotolerant acidophilic Streptomyces. The effect of geographic separation was clearly seen among the Streptacidiphilus isolates, which may be a key factor in speciation of the genus.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.29 no.2 s.233
• /
• pp.228-231
• /
• 2005

In this paper, we present reusable quartz master fabricated by electron-beam lithography and dry etching process of quartz, and results of injection molding based on the reusable quartz master for the manufacturing of nano-scale information media. Since patterned structures of photoresist can be easily damaged by separation (demolding) process of nickel stamper and master, a master with photoresist cannot be reused in stamper fabrication process. In this work, we have made it possible of the repeated use of master by directly patterning on quart in nickel stamper fabrication process. We have designed and fabricated four different specimens including 100nm, 140nm 200nm and 400nm pit patterns. In addition, both intaglio and embossed carving patterns are fabricated for each specimen. In the preliminary test of injection molding, we have fabricated polycarbonate patterns with varying mold temperature. We have experimentally verified the fabrication process of the reusable quart master and possibility of quartz master as direct stamper.