• Title/Summary/Keyword: Separation condition

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Purification of Lysozyme from Egg White by Multicycle Ion Exchange Chromatography (다중 이온교환크로마토그래피를 이용한 계란난백에서 리소짐의 분리)

  • 허윤석;김형원;김인호
    • KSBB Journal
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    • v.18 no.2
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    • pp.122-126
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    • 2003
  • Multi-cycle chromatographic separation of Iysozyme from egg white was investigated. Multi-cycle chromatography was performed by repeated cycling(one cycle: resin equilibration, sample loading, washing, elution). Two types of cation exchange resins, Cellufine CM C-200 and Bio-rex 70, were used to determine the optimum condition for the separation of Iysozyme by multi-cycle chromatography. The resin was equilibrated in 20 mM Na-phosphate buffer(pH 7.0). Chromatograms of UV absorbance levels of every cycle were compared to confirm the eluting ability of Iysozyme in the two types of gel. Collected samples from eluting regions in every cycle were assayed by 15% SDS-PAGE.

The Effect of Mold Shapes on the Fiber Orientation of Welding Parts for Injectin Molding of Fiber Reinforced Polymeric Composites (섬유강화 고분자 복합재료의 사출성형에 있어서 웰드부의 섬유배향에 미치는 금형형상의 영향)

  • Kang, M. G.;Choi, Y. S.;Kim, H.;Lee, D. G.;Han, G. Y.;Kim, E. G.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2000.11a
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    • pp.457-460
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    • 2000
  • Injection molding is the most widely used process for the industrial forming of plastic articles. During an injection molding process of composites, the fiber-matrix separation and fiber orientation are caused by the flow of molten polymer/fiber mixture. As a result, the product tends to be nonhomogeneous and anisotropic. Hence, it is very important to clarify the relations between separation·orientation and infection molding conditions. So far, there is no research on the measurement of fiber orientation using image processing. In this study, the effects of fiber content ratio and molding condition on the fiber orientation-angle distributions are studied experimentally. Using the image processing method, the fiber orientation distribution of welding parts in injection-molded products is assessed. And the effects of fiber content and injection mold shapes on the fiber orientation in case of fiber reinforced polymeric composites are studied. experimentally.

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Effect of impingement edge geometry on the acoustic resonance excitation and Strouhal numbers in a ducted shallow cavity

  • Omer, Ahmed;Mohany, Atef;Hassan, Marwan
    • Wind and Structures
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    • v.23 no.2
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    • pp.91-107
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    • 2016
  • Flow-excited acoustic resonance in ducted cavities can produce high levels of acoustic pressure that may lead to severe damage. This occurs when the flow instability over the cavity mouth, which is created by the free shear layer separation at the upstream edge, is coupled with one of the acoustic modes in the accommodating enclosure. Acoustic resonance can cause high amplitude fluctuating acoustic loads in and near the cavity. Such acoustic loads could cause damage in sensitive applications such as aircraft weapon bays. Therefore, the suppression and mitigation of these resonances are very important. Much of the work done in the past focused on the fluid-dynamic oscillation mechanism or suppressing the resonance by altering the edge condition at the shear layer separation. However, the effect of the downstream edge has received much less attention. This paper considers the effect of the impingement edge geometry on the acoustic resonance excitation and Strouhal number values of the flow instabilities in a ducted shallow cavity with an aspect ratio of 1.0. Several edges, including chamfered edges with different angles and round edges with different radii, were investigated. In addition, some downstream edges that have never been studied before, such as saw-tooth edges, spanwise cylinders, higher and lower steps, and straight and delta spoilers, are investigated. The experiments are conducted in an open-loop wind tunnel that can generate flows with a Mach number up to 0.45. The study shows that when some edge geometries, such as lower steps, chamfered, round, and saw-tooth edges, are installed downstream, they demonstrate a promising reduction in the acoustic resonance. On the other hand, higher steps and straight spoilers resulted in intensifying the acoustic resonance. In addition, the effect of edge geometry on the Strouhal number is presented.

Performance evaluation of forward osmosis (FO) hollow fiber module with various operating conditions (중공사막 모듈을 이용한 정삼투 공정에서의 운영조건 변화에 따른 성능평가)

  • Kim, Bongchul
    • Journal of Korean Society of Water and Wastewater
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    • v.32 no.4
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    • pp.357-361
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    • 2018
  • Forward osmosis (FO) process has been attracting attention for its potential applications such as industrial wastewater treatment, wastewater reclamation and seawater desalination. Particularly, in terms of fouling reversibility and operating energy consumption, the FO process is assumed to be preferable to the reverse osmosis (RO) process. Despite these advantages, there is a difficulty in the empirical step due to the lack of separation and recovery techniques of the draw solution. Therefore, rather than using FO alone, recent developments of the FO process have adapted a hybrid system without draw solution separation/recovery systems, such as the FO-RO osmotic dilution system. In this study, we investigated the performance of the hollow fiber FO module according to various operating conditions. The change of permeate flow rate according to the flow rates of the draw and feed solutions in the process operation is a factor that increases the permeate flow rate, one of the performance factors in the positive osmosis process. Our results reveal that flow rates of draw and feed solutions affect the membrane performance, such as the water flux and the reverse solute flux. Moreover, use of hydraulic pressure on the feed side was shown to yield slightly higher flux than the case without applied pressure. Thus, optimizing the operating conditions is important in the hollow fiber FO system.

A Convenient Method on the Methyl-Ethyl-Ketone Extraction of $^{99m}TcO^-{_4}$ ($^{99m}TcO^-{_4}$의 메틸-에틸-케톤-간편 추출법)

  • Lee, Jong-Du;Lee, Byung-Hyn
    • Journal of Radiation Protection and Research
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    • v.9 no.2
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    • pp.103-111
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    • 1984
  • A convenient method of $^{99m}Tc$-methyl-ethyl-ketone (MEK) extraction technique was developed and a mobile $^{99m}Tc$-extraction generator was designed. The MEK extraction and the phase separation of $^{99m}TcO^-{_4}$ were carried out with a simple procedure in the same container. The shielding of $^{99}Mo$ radioactivity was made with one lead container. The system was simplified by shielding $^{99m}TcO_4{^-}({\gamma}_e=0.14\;MeV)$ separately. $^{99m}TcO^-{_4}\;in\;^{99m}Tc-MEK$ extract was recovered by adsorption and elution only, and therefore, the possibility of volatilization was reduced. The volume of $^{99m}TcO^{-}{_4}$-saline product was reduced to 1 ml by using a small alumina column and the column operation time was shortened. The separation time of $^{99m}Tc$ was reduced to 30 minutes, and the operation was carried out at the outside of the shielding. The system was designed to operate under the condition of bacteria-free.

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Measurement of the Flow Field Around a Quadcopter in Vertical Descending Flight (수직 하강 비행 조건에서의 쿼드콥터 주위의 유동장 계측)

  • Kwon, Min-Jeong;Kwon, Ki-Jung
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.46 no.5
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    • pp.359-367
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    • 2018
  • The vortex ring state that occurs during the descending flight of a rotorcraft generates a circulating flow like a donut near the rotating surface, and it often causes a rotorcraft fall due to loss of thrust. In this paper, we have physically identified the flow field in the vortex ring state of the quadcopter, one of the types of unmanned aerial vehicles. The descending flight of the quadcopter was simulated in a 1m subsonic wind tunnel of the Korea Aerospace Research Institute(KARI) and the Particle Image Velocimetry(PIV) was used for the flow field measurement. The induced velocity in the hovering state is estimated by using the momentum theory and the test was carried out in the range of descent rate at which the vortex ring condition could be caused. The development and the direction of the vortex ring were confirmed by the measurement of the flow field according to not only the descent rate but also propeller separation distance. In addition, the results of the study show the vortex ring state can be predicted sufficiently by measuring the flow velocity around the quadcopter.

Study on As Removal from Mine Tailing using MGS Gravity Separator (다중비중선별기를 이용한 광물찌꺼기로부터 비소제거 연구)

  • Kim, Jun-Hee;Kim, Min-Gyu;Kim, Woo-Ram;Han, Oh-Hyung
    • Resources Recycling
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    • v.23 no.5
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    • pp.36-43
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    • 2014
  • It comtained various kinds of heavy metal in beneficiatian tailing and in order to eliminate the heavy metals, various technologies have been researched, But there are a limits because many problems and restrictions are accompanied. Therefore, in this study, in order to solve this problem, It was performed gravity separation using melti-gravity seperator (MGS) to remove As contained in tailing, which can sort out even the fine particle. It was attempted a study to remove As, which is a kind of heavy metals, by MGS gravity separation and verified the impact by slope, wash water, pulp density, rotational speed and feed rate. In the results of performing gravity concentration under the condition with the MGS slope of $3^{\circ}$, wash water of 5 l/mm, pulp density of 30%, rotational speed of 208 rpm, and the feed rate of 265.24 g/mm, the As removal rate of 88.21% and yield of 92.25% were obtained.

Simulation of Particle Behaviors within a Multi-stage Impact Crusher using Discrete Element Method (이산요소법을 이용한 다단 임팩트 파쇄기 내 입자 거동 모사)

  • Yu, Myoungyuol;Lee, Hoon
    • Resources Recycling
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    • v.27 no.3
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    • pp.86-92
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    • 2018
  • The amount of construction waste generated is steadily increasing every year, and the Law for Promotion of Recycling is enacted. However, it is difficult to use it as a recycled aggregate for concrete, which is presented in the quality standard of recycled aggregate with high water uptake and low density due to low separation of aggregate between concrete and cement paste. Therefore, in this study, a multi-stage impact crusher was used to remove mortar, which is essential for improving the quality of recycled aggregate. In analyzing the characteristics of the equipment, the spectrum of energy generated in each part between the particle and the equipment was calculated by using DEM. In order to generate an effective separation phenomenon, it was confirmed that the operation condition of 900 RPM was appropriate based on the ratio of the number of collisions (L/H) of the low energy group (L) to the number of collisions of the high energy group (H).

The assessment of the Spatial Variation of the Wind Field using the Meso-velocity Scale and its Contributing Factors (중간 속도 규모를 이용한 바람장의 균질성 평가 및 영향요소 분석)

  • Lee, Seong-Eun;Shin, Sun-Hee;Ha, Kyung-Ja
    • Atmosphere
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    • v.20 no.3
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    • pp.343-353
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    • 2010
  • A regional wind network with complex surface conditions must be designed with sufficient space and time resolution to resolve the local circulations. In this study, the spatial variations of the wind field observed in the Seoul and Jeju regional networks were evaluated in terms of annual, seasons, and months to assess the spatial homogeneity of wind fields within the regional networks. The coherency of the wind field as a function of separation distance between stations indicated that significant coherency was sometimes not captured by the network, as inferred by low correlations between adjacent stations. A meso-velocity scale was defined in terms of the spatial variability of the wind within the network. This problem is predictably most significant with weak winds, dull prevailing wind, clear skies and significant topography. The relatively small correlations between stations imply that the wind at a given point cannot be estimated by interpolating winds from the nearest stations. For the Seoul and Jeju regional network, the meso-velocity scale has typically a same order of magnitude as the speed of the network averaged wind, revealing the large spatial variability of the Jeju network station imply topography and weather. Significant scatter in the relationship between spatial variability of the wind field and the wind speed is thought to be related to thermally-generated flows. The magnitude of the mesovelocity scale was significantly different along separation distance between stations, wind speed, intensity of prevailing wind, clear and cloudy conditions, topography. Resultant wind vectors indicate much different flow patterns along condition of contributing factors. As a result, the careful considerations on contributing factors such as prevailing wind in season, weather, and complex surface conditions with topography and land/sea contrast are required to assess the spatial variations of wind field on a regional network. The results in the spatial variation from the mesovelocity scale are useful to represent the characteristics of regional wind speed including lower surface conditions over the grid scale of large scale atmospheric model.

Analysis and the Conservation Treatment of the Paper Relics of the Seonwon Temple Area (선원사지(禪源寺地)출토 지류 유물의 분석 및 보존 처리 방법)

  • Lim, Ju-Hee;Park, Chi-sun
    • Journal of Conservation Science
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    • v.12 no.1 s.15
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    • pp.71-82
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    • 2003
  • The paper relics excavated from the 4th excavation of the historical spot No.259 Seonwon temple area were analyzed and conserved. Because these works remained folded, the most important conservation treatment was a separation. This process depended on the state of the works: dry state works were separated using Gore-Tex chamber and the filtrated water, wet state works were separated through vacuum freeze drying method. Analyses of the paper relics performed were examination on the paper to find its distinction, binocular microscope and SEM on fiber, and XRF on character ingredient. This study presumed the times though $C^{14}$ dating of these works. The works were able to be classified through analysis and conservation treatment. This separation made lost part of the works conserve an archetype. This study presented the application of conservation methods on the paper relics which are in the bad condition and obtained information through carbon dating of it.

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