• Environmental Engineering Research
• /
• v.13 no.4
• /
• pp.216-223
• /
• 2008

An efficient method for the simultaneous determination of eleven phenolic endocrine-disrupting chemicals (EDCs) present in wastewater influent samples was described. The 11 phenolic EDCs including alkylphenols, chlorophenols, and bisphenol A were determined by gas chromatography/mass spectrometry-selected ion monitoring (GC/MS-SIM) following two work-up methods for comparison; isobutoxycarbonyl (isoBOC) derivatization and tert-butyldimethylsilyl (TBDMS) derivatization. The wastewater influent samples containing the 11 EDCs were adjusted to pH 2 with $H_2SO_4$ and then cleaned up with n-hexane. Next, they were subjected to solid-phase extraction (SPE) with XAD-4 resin and subsequently converted to isoBOC or TBDMS derivatives for sensitivity analysis with gas chromatography/mass spectrometry-selected ion monitoring (GC/MSSIM). Following isoBOC derivatization and TBDMS derivatization, the recoveries were 86.6-105.2% and 97.6-142.7%, the limits of quantitation (LOQ) for the 11 phenolic EDCs for SIM was 0.001-0.050 ng/mL and 0.003-0.050 ng/mL, and the SIM responses were linear with the correlation coefficient varying by 0.9717-0.9995 and 0.9842-0.9980, respectively. When these methods were applied to five selected wastewater influent samples, for isoBOC derivatization and TBDMS derivatization the ranges of concentration detected were 0.2-99.6 ng/mL and 0.4-147.4 ng/mL, respectively.

• Journal of Korea Multimedia Society
• /
• v.7 no.12
• /
• pp.1680-1691
• /
• 2004

One of the representative methods of optical flow is a gradient method which estimates the movement of an object based on the differential of image brightness. However, the method is ineffective for large displacement of the object and many improved methods have been proposed to copy with such limitations. One of these improved techniques is the multigrid processing, which is used in many optical flow algorithms. As an alternative novel technique we have been proposing an orthogonal functional expansion method, where whole displacements are expanded from low frequency terms. This method is expected to be applicable to flow estimation with large displacement and deformation including expansion and contraction, which are difficult to cope with by conventional optical flow methods. In the orthogonal functional expansion method, the apparent displacement field is calculated iteratively by a projection method which utilizes derivatives of the invariant constraint equations of brightness constancy. One feature of this method is that differentiation of the input image is not necessary, thereby reducing sensitivity to noise. In this paper, we apply our method to several real images in which the objects undergo large displacement and/or deformation including expansion. We demonstrate the effectiveness of the orthogonal functional expansion method by comparing with conventional methods including our optimally scaled multigrid optical flow algorithm.

• Transactions of the Korean Society of Machine Tool Engineers
• /
• v.10 no.2
• /
• pp.29-37
• /
• 2001

The application of laser in pipelines was started from the base of using laser in nuclear facilities industries and power plants. Because laser can be delivered to a remote area without any difficulties, the application of laser in many industries can solve many difficulties from limitation of access in danger area and reduced the risks of workers. Therefore, we developed a new experimental technique to measure internal defects of pressure vessels with a combination of shearog-raphy and image processing technique. Conventional NDT methods have been taken relatively much time, money and manpower because of performing as the method of contact with objects to be inspected. But digital shearography is laser-based optical method which allows full-field observation of surface displacement derivatives. This method has many advantages in practical use, such as low sensitivity to environmental noise, simple optical configuration and real time mea-surement. In this paper, we find the optimum shearing magnitude with EFM and experiment and measured internal crack length of the pressure vessels at a real time and estimated the error of the results.

• The Journal of the Acoustical Society of Korea
• /
• v.15 no.2
• /
• pp.95-100
• /
• 1996

In this paper, we present an image coder using variable quantizer for newly proposed JPEG extensions which has been standardized as ISO/IEC 10918-3(ITU-T Rec. T.84). It is necessary to alleviate the blocking artifact which is more sensitive to human eye in view of the spatial frequency sensitivity. The blocking artifact arises in the lower activity area rather than in the higher area. Therefore variable quantizer use the horizontal and vertical derivatives for calculating the $8{\times}8$ block activity. We classified nonlinear quantizer parameter into 5 categories in order to finely quantize in the lower active region. As a result of simulation for various images, the proposed coder increases subjective and objective quality at a given bit rate.

• Biomolecules & Therapeutics
• /
• v.21 no.1
• /
• pp.1-9
• /
• 2013

Polyamines, putrescine, spermidine and spermine, are ubiquitous in living cells and are essential for eukaryotic cell growth. These polycations interact with negatively charged molecules such as DNA, RNA, acidic proteins and phospholipids and modulate various cellular functions including macromolecular synthesis. Dysregulation of the polyamine pathway leads to pathological conditions including cancer, inflammation, stroke, renal failure and diabetes. Increase in polyamines and polyamine synthesis enzymes is often associated with tumor growth, and urinary and plasma contents of polyamines and their metabolites have been investigated as diagnostic markers for cancers. Of these, diacetylated derivatives of spermidine and spermine are elevated in the urine of cancer patients and present potential markers for early detection. Enhanced catabolism of cellular polyamines by polyamine oxidases (PAO), spermine oxidase (SMO) or acetylpolyamine oxidase (AcPAO), increases cellular oxidative stress and generates hydrogen peroxide and a reactive toxic metabolite, acrolein, which covalently incorporates into lysine residues of cellular proteins. Levels of protein-conjuagated acrolein (PC-Acro) and polyamine oxidizing enzymes were increased in the locus of brain infarction and in plasma in a mouse model of stroke and also in the plasma of stroke patients. When the combined measurements of PC-Acro, interleukin 6 (IL-6), and C-reactive protein (CRP) were evaluated, even silent brain infarction (SBI) was detected with high sensitivity and specificity. Considering that there are no reliable biochemical markers for early stage of stroke, PC-Acro and PAOs present promising markers. Thus the polyamine metabolites in plasma or urine provide useful tools in early diagnosis of cancer and stroke.

• Journal of Electrical Engineering and Technology
• /
• v.4 no.1
• /
• pp.106-110
• /
• 2009

An amperometric glucose biosensor has been developed using viologen derivatives as a charge transfer mediator between a glucose oxidase (GOD) and a gold electrode. A highly stable self-assembled monolayer (SAM) of thiol-based viologen was immobilized onto the gold electrode of a quartz crystal microbalance (QCM) and GOD was immobilized onto the viologen modified electrode. This biosensor response to glucose was evaluated amperometrically in the potential of -300mV. Upon immobilization of the glucose oxidase onto the viologen modified electrode, the biosensor showed rapid response towards glucose. Experimental conditions influencing the biosensor performance, such as pH potential, were optimized and assessed. This biosensor offered excellent electrochemical responses for glucose concentration below ${\mu}$ mol level with high sensitivity and selectivity and short response time. The levels of the RSDs (<5%) for the entire analyses reflected the highly reproducible sensor performance. A linear calibration range between the current and the glucose concentration was obtained up to $4.5{\times}10^{-4}M$. The detection limit was determined to be $3.0{\times}10^{-6}M$.

• Proceedings of the KIEE Conference
• /
• 2007.11a
• /
• pp.86-87
• /
• 2007

An amperometric glucose biosensor has been developed using viologen derivatives as electron mediator of glucose oxidase (GOD) at Au electrode. Highly stable self assembled monolayer (SAM) of thiol-based viologen is immobilized onto the Au electrode followed byGOD is immobilized onto the viologen modified electrode. This biosensor response to glucose was evaluated amperometrically in the potential of -300 mV. Upon immobilization of glucose oxidase onto the viologen modified-electrode, the biosensor showed rapid response towards glucose. Experimental conditions influencing the biosensor performance such as, pH, potential were optimized and assessed. This biosensor offered an excellent electrochemical response for glucose concentration below ${\mu}mol$ level with high sensitivity and selectivity and short response time. The levels of the RSD's (< 5 %) for the entire analyses reflected the highly reproducible sensor performance. Using the optimized a linear relationship between current and glucose concentration was obtained up to $4.5{\times}10^{-4}$ M. In addition, this biosensor showed well reproducibility and stability.

• Archives of Pharmacal Research
• /
• v.26 no.9
• /
• pp.697-705
• /
• 2003

The alkylphenols, chlorophenols, and bisphenol A were determined by gas chromatography/mass spectrometry-selected ion monitoring (GC/MS-SIM) followed by two work-up methods for comparison: isobutoxycarbonyl (isoBOC) derivatization and tert-butyldimethylsilyl (TBDMS) derivatization. Eleven endocrine disrupting chemicals (EDCs) of phenols in biological samples were extracted with acetonitrile and then the acetonitrile layer underwent freezing filtration 6$0^{\circ}C$ for 2 hours. Solid-phase extraction (SPE) was used with XAD-4 and subsequent conversion to isoBOC or TBDMS derivatives for sensitivity analysis with the GC/MS-SIM mode. For isoBOC derivatization and TBDMS derivatization the recoveries were 92.3∼150.6% and 93.8∼108.3%, the method detection limits (MDLs) of bisphenol A for SIM were 0.062 $\mu$ g/kg and 0.010 $\mu$ g/kg, and the SIM responses were linear with the correlation coefficient varying by 0.9755∼0.9981 and 0.9908∼0.9996, respectively. When these methods were applied to mackerel samples, the concentrations of the 11 phenol EDCs were below the MDL.

• Advances in Computational Design
• /
• v.7 no.1
• /
• pp.81-98
• /
• 2022

This study presents the application of two indices for the locating of cracks in Reinforced Concrete (RC) structures, as well as the development of their modified forms to overcome limitations. The first index is based on mode shape curvature and the second index is based on the fourth derivative of the mode shape. In order to confirm the indices' effectiveness, both eigenvalues coupled with nonlinear static analyses were carried out and the eigenvectors for two different damage locations and intensities of load were obtained from the finite element model of RC beams. The values of the damage-locating indices derived using both indices were then compared. Generally, the mode shape curvature-based index suffered from insensitivity when attempting to detect the damage location; this also applied to the mode shape fourth derivative-based index at lower modes. However, at higher modes, the mode shape fourth derivative-based index gave an acceptable indication of the damage location. Both the indices showed inconsistencies and anomalies at the supports. This study proposed modification to both indices to overcome identified flaws. The results proved that modified forms exhibited better sensitivity for identifying the damage location. In addition, anomalies at the supports were eliminated.

• Journal of Electrical Engineering and information Science
• /
• v.1 no.2
• /
• pp.77-86
• /
• 1996

In this paper we deal with the problem of recovering 3-D motion and structure from a time-varying 2-D velocity vector field. A great deal has been done on this topic, most of which has concentrated on finding necessary and sufficient conditions for there to be a unique 3-D solution corresponding to a given 2-D motion. While previous work provides useful theoretical insight, in most situations the known algorithms have turned out to be too sensitive to be of much practical use. It appears that any robust algorithm must improve the 3-D solutions over time. As a step toward such algorithm, we present a method for recovering 3-D motion and structure from a given time-varying 2-D velocity vector field. The surface of the object in the scene is assumed to be locally planar. It is also assumed that 3-D velocity vectors are piecewise constant over three consecutive frames (or two snapshots of flow field). Our formulation relates 3-D motion and object geometry with the optical flow vector as well as its spatial and temporal derivatives. The linearization parameters, or equivalently, the first-order flow approximation (in space and time) is sufficient to recover rigid body motion and local surface structure from the local instantaneous flow field. We also demonstrate, through a sensitivity analysis carried out for synthetic and natural motions in space, that 3-D motion can be recovered reliably.