• Journal of Microbiology
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• 제45권4호
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• pp.339-343
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• 2007

Methylglyoxal (MG) is a reactive metabolite known to accumulate in certain physiological conditions. We attempted to isolate genes associated with this metabolite by genome-wide mutagenesis with TnphoA derivative. After screening on methylglyoxal-containing plate, we obtained insertions in three different genes, ydbD, yjjQ, and yqiI, which gave rise to reproducible MG-sensitive phenotypes in glyoxalase-deficient strain. In addition to its MG sensitivity, the insertion in yqiI exhibited an impaired motility resulting from a reduced flagellar expression.

• 한국조명전기설비학회:학술대회논문집
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• 한국조명전기설비학회 2004년도 춘계학술대회 논문집
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• pp.559-564
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• 2004

This paper deals with the device for measuring the time-varying magnetic fields and induced voltages caused by lightning discharges. The two magnetic field measuring systems were designed and made. One consists of the loop-type magnetic field sensor with the active integrator operated by a differential amplifier. The other consists of the loop-type magnetic field sensor and Labview software. The loop-type magnetic field sensor detects the time derivative of the magnetic field being measured, and the signal detected is integrated by the Labview software. As a consequence, from the calibration experiments, the frequency bandwidth of the full measuring system ranges from 400 [Hz] to 1 (MHz) and the response sensitivity are 0.98 (mV/nT) and 22 (mV/nT) for the magnetic field sensor of 2 turns and 6 turns, respectively. Also, the results obtained by the two measuring devices well agreed with each other.

• 대한화학회지
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• 제51권4호
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• pp.331-338
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• 2007

분석 시약으로 2-hydroxy-3-methoxy benzaldehyde thiosemicarbazone (HMBATSC)를 사용하여 코발트 (II) 정량을 위하여 빠르고 간단하고 민감한 분광광도법을 개발하였다. 액상에서 금속이온이 pH 6.0에서 HMBATSC와 갈색의 착물을 형성하였다. 이 착물은 375 nm와 390 nm에서 두 개의 최대 흡수 피크를 보였다. 375 nm에서 많은 흡 수를 보였고, 반면 390 nm에서는 분명한 흡수를 보이지 않았다. Beer's 법칙에서 Co(II)는 0.059-2.357 μg ml-1 범위를 보였다. 이 방법의 몰 흡수와 Sandall's 민감도는 각각 2.74×104 l mol-1 cm-1와 0.0024 μg cm-2 였다. 여러 가지 다른 이온들에 간섭에 대해서도 연구하였다. 화학양론적으로 착체는 1:2 [Co(II)- HMBATSC] 이 였다. 이차 유도 분광광도 법에 의한 Co(II)의 정량방법에 대해서도 제안하였다. 제안된 방법을 alloy steels, 비타민 B12와 생물학 시료에 있는 Co(II)의 정량에 적용하였다.

• 한국식품위생안전성학회지
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• 제9권3호
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• pp.163-168
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• 1994

In the present study we investigated the peripheral function of capsaicin and KR-25018, a newly synthesized capsaicin derivative, which was demonstrated to have a potent analgesic activity through different mechanism from morphine and nonsteroidal antiinflammatory drugs. Capsaicin (10-8~10-5 M) and KR-25018 (10-8~10-5 M) produced concentration-dependent contractions of the isolated guinea pig bronchi. There were no significant differences in the maximum response and the EC50 values (EC50: 0.137$\pm$0.025 $\mu$M and 0.097$\pm$0.031 $\mu$M for capsaicin and KR-25018, respectively, P>0.05). Phosphoramidon (10 $\mu$M) and indomethacin (10 $\mu$M) had no significant effect on contractile response to the submaximal concentration range of capsaicin and KR-25018 (3$\times$10-9~3$\times$10-7 M). The response to KR-25018, like that to capsaicin, was significantly inhibited by ruthenium red with reduction in the maximum response, which is indicative of non-competitive antagonism. A further common feature of the responses to capsaicin and KR-25018 in the guinea pig bronchi was their sensitivity to capsazepine. Capsazepine caused a rightward parallel shift in concentration-response curves obtained by capsaicin and KR-25018. the pA2 values of capsazepine were 5.90 and 5.99 against capsaicin and KR-25018 response, respectively. In conclusion, KR-25018 and capsaicin exert their contractile effects in the isolated guinea pig bronchial muscle by common mechanisms, probably via the activation of a specific receptor.

• 대한유전성대사질환학회지
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• 제15권3호
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• pp.138-146
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• 2015

Purpose: If early diagnosis is not made, patients with metabolic disorders as homocystinemia rapidly progress to physical defect or mental retardation resulted in storage of the toxic material into the brain. Therefore, it is necessary to develop an analytical method for a rapid screening and/or correct confirmation diagnosis. Methods: The standard solution of sulfur amino acids spiked plasma was subjected to protein precipitation with methanol, and then consecutively derivatized with trimethylsilyl (TMS) and trifluoroacyl (TFA) and determined by GC-MS. The formation of TMS derivative of the hydroxyl and TFA derivative of amino functional group was performed by BSTFA and MBTFA, respectively. Selective ion monitoring (SIM) mode was used for quantification with selected specific ions. Results: A calibration curve on standard spiked pooled plasma showed a linear relationship with correlation coefficient of 0.9936-0.9992 for all compounds over the range of 0.1-300 ng. The precision and accuracy were within S.D. of 1-15% and RSD of 1-15% for intra-day assay at 2 ng/mL, 15 ng/mL and 30 ng/mL. LOD and LOQ was 0.4 ng/mL and 4 ng/mL respectively. Conclusion: A rapid analytical method was developed to quantify sulfur amino acids and methyl malonic acid, after two-step derivatization procedure with good sensitivity and specificity on human plasma. Advantages of a new method are simplicity and rapidity. The method could be useful for routine analysis, diagnosis of homocysteinemia.

• Toxicological Research
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• 제20권2호
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• pp.89-100
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• 2004

The comet assay (also called the single-cell gel electrophoresis assay) has been widely used for detecting DNA damage and repair in individual cells. Since the conventional methods of evaluating comet assay data using frequency statistics are unsatisfactory we developed a new quantitative measure of DNA damage/repair that is based on all information residing in the dose/time-response curves of a comet experiment. Blood samples were taken from 25 breast cancer patients before undergoing radiotherapy. The comet assay was performed under alkaline conditions using isolated lymphocytes. Tail DNA, tail length, tail moment and tail inertia of the comet were measured for each patient at four doses of $\gamma$-rays (0, 2, 4 and 8 Gy) and at four time points after irradiation (0, 10, 20 and 30 min) using 100 cells each. The resulting three-dimensional dose-time response surface was modeled by multiple regression, and the second derivative, termed 2D, on dose and time was determined. A software module was programmed in SAS/AF to compute 2D values. We applied the new method successfully to data obtained from cancer patients to be assessed for their radiation sensitivity. We computed the 2D values for the four damage measures, i.e., tail moment, tail length, tail DNA and tail inertia, and examined the pairwise correlation coefficients of 2D both on the log scale and the unlogged scale. 2D values based on tail moment and tail DNA showed a high correlation and, therefore, these two damage measures can be used interchangeably as far as DNA repair is concerned. 2D values based on tail inertia have a correlation profile different from the other 2D values which may reflect different facets of DNA damage/repair. Using the dose-time response surface, other statistical models, e.g., the proportional hazards model, become applicable for data analysis. The 2D approach can be applied to all DNA repair measures, Le., tail moment, tail length, tail DNA and tail inertia, and appears to be superior to conventional evaluation methods as it integrates all data of the dose/time-response curves of a comet assay.

• 한국전산구조공학회논문집
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• 제27권1호
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• pp.9-16
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• 2014

레벨셋 기법과 위상민감도를 이용하여 선형 탄성 구조물에 대하여, 초기 설계형상에 의존성이 없는 위상 및 형상 최적설계 기법을 개발하였다. 레벨셋 기법에서는 복잡한 위상 형상변화를 쉽게 다루기 위해 초기 영역은 고정한 채 레벨셋 함수로 표현되는 암시적 이동경계로 경계를 표현한다. 해밀턴-자코비(H-J) 방정식과 수치적으로 강건한 기법인 'up-wind scheme'은 컴플라이언스 목적함수를 최소화시키고 허용체적 제약조건을 만족시키면서, 초기 암시적 경계를 법선 속도장에 따라 최적의 형상으로 이끌어 낸다. 점근적인 정규화 개념에 근거하여, 구멍의 반지름을 0으로 접근시켜 형상 미분의 극한을 취한 위상민감도를 고려하였다. 최적조건으로부터 유도된 라그란지안의 감소 방향을 이용하여 H-J 방정식을 갱신하기 위한 속도장을 결정하였다. 개발한 방법에서는 위상민감도로부터 얻어지는 지표를 이용하여 구멍을 언제든지 어디에서나 생성가능하기 때문에 초기 구멍이 최적 형상을 얻기 위해 요구되지 않는다는 사실을 확인하였다. 또한 효율적인 최적화 과정을 위해서는 구멍 생성을 위한 조정변수의 적절한 선택이 중요함을 확인하였다.

• 한국전산구조공학회논문집
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• 제27권5호
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• pp.337-343
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• 2014

본 논문에서는 재생 커널 기법을 사용하여 혼합모드 균열진전 문제에 대한 연속체 기반의 형상 설계민감도 해석을 수행하였다. 재생 커널 기법은 기존의 유한요소법과 달리 요소망을 재구성할 필요가 없어, 커널 함수의 연속성을 증가시켰을 때 높은 정밀도의 형상함수를 얻을 수 있다는 장점을 가지고 있다. 균열선단 주변에서 J-적분을 수행하기 위해 선형탄성 조건이 고려되었다. 변위장과 응력 확대 계수의 설계변수에 대한 감도해석을 위하여 물질도함수를 도입하였으며 직접 미분법보다 효율적인 애조인 방법을 사용하여 설계민감도를 유도하였다. 수치 예제들을 통해서 재생 커널 기법을 이용한 균열진전 해석결과의 타당성을 확인하였으며 애조인 방법을 이용한 형상 설계민감도 해석 결과를 유한차분법과 비교하여 매우 정확하고 효율적인 결과를 얻을 수 있음을 알 수 있었다. 이를 바탕으로 간단한 모델에 대하여 형상 최적설계를 수행하여 균열이 발생될 수 있는 구조물에 대해서 균열에 의한 피해를 최소화할 수 있도록 균열을 제어할 수 있는 최적의 형상을 도출하였다.

• 대한의용생체공학회:의공학회지
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• 제43권4호
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• pp.280-289
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• 2022

In this paper, we present a QRS-complex detection algorithm to calculate an accurate heartbeat and clearly recognize irregular rhythm from ECG signals. The conventional Pan-Tompkins algorithm brings false QRS detection in the derivative when QRS and noise signals have similar instant variation. The proposed algorithm uses amplitude differences in 7 adjacent samples to detect QRS-complex which has the highest amplitude variation. The calculated amplitude is cubed to dominate QRS-complex and the moving average method is applied to diminish the noise signal's amplitude. Finally, a decision rule with a threshold value is applied to detect accurate QRS-complex. The calculated signals with Pan-Tompkins and proposed algorithms were compared by signal-to-noise ratio to evaluate the noise reduction degree. QRS-complex detection performance was confirmed by sensitivity and the positive predictive value(PPV). Normal ECG, muscle noise ECG, PVC, and atrial fibrillation signals were achieved which were measured from an ECG simulator. The signal-to-noise ratio difference between Pan-Tompkins and the proposed algorithm were 8.1, 8.5, 9.6, and 4.7, respectively. All ratio of the proposed algorithm is higher than the Pan-Tompkins values. It indicates that the proposed algorithm is more robust to noise than the Pan-Tompkins algorithm. The Pan-Tompkins algorithm and the proposed algorithm showed similar sensitivity and PPV at most waveforms. However, with a noisy atrial fibrillation signal, the PPV for QRS-complex has different values, 42% for the Pan-Tompkins algorithm and 100% for the proposed algorithm. It means that the proposed algorithm has superiority for QRS-complex detection in a noisy environment.

• Structural Engineering and Mechanics
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• 제32권5호
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• pp.667-683
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• 2009

FE model-based dynamic analysis has been widely used to predict the dynamic characteristics of civil structures. In a physical point of view, an FE model is unavoidably different from the actual structure as being formulated based on extremely idealized engineering drawings and design data. The conventional model updating methods such as direct method and sensitivity-based parameter estimation are not flexible for model updating of complex and large structures. Thus, it is needed to develop a model updating method applicable to complex structures without restriction. The main objective of this paper is to present the model updating method based on the hybrid genetic algorithm (HGA) by combining the genetic algorithm as global optimization method and modified Nelder-Mead's Simplex method as local optimization method. This FE model updating method using HGA does not need the derivation of derivative function related to parameters and without application of complicated inverse analysis methods. In order to allow its application on diversified and complex structures, a commercial FEA tool is adopted to exploit previously developed element library and analysis algorithms. Moreover, an output-level objective function making use of measurement and analytical results is also presented to update simultaneously the stiffness and mass of the analysis model. The numerical examples demonstrated that the proposed method based on HGA is effective for the updating of the FE model of bridge structures.