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Screening of Genes Related to Methylglyoxal Susceptibility  

Kim, In-Sook (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
Kim, Joon-Ho (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
Min, Bum-Chan (Analytical Science Center, Samyang Central R&D Institute)
Lee, Chang-Han (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
Park, Chan-Kyu (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
Publication Information
Journal of Microbiology / v.45, no.4, 2007 , pp. 339-343 More about this Journal
Abstract
Methylglyoxal (MG) is a reactive metabolite known to accumulate in certain physiological conditions. We attempted to isolate genes associated with this metabolite by genome-wide mutagenesis with TnphoA derivative. After screening on methylglyoxal-containing plate, we obtained insertions in three different genes, ydbD, yjjQ, and yqiI, which gave rise to reproducible MG-sensitive phenotypes in glyoxalase-deficient strain. In addition to its MG sensitivity, the insertion in yqiI exhibited an impaired motility resulting from a reduced flagellar expression.
Keywords
genome screening; methylglyoxal; dihydroxyacetone; glyoxalase;
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