• Journal of Microbiology and Biotechnology
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• 제34권6호
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• pp.1322-1327
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• 2024

The accurate and rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) holds significant clinical importance. This work presents a new method for detecting methicillin-resistant Staphylococcus aureus (S. aureus) in clinical samples. The method uses an aptamer-based colorimetric assay that combines a recognizing probe to identify the target and split DNAzyme to amplify the signal, resulting in a highly sensitive and direct analysis of methicillin-resistance. The identification of the PBP2a protein on the membrane of S. aureus in clinical samples leads to the allosterism of the recognizing probe, and thus provides a template for the proximity ligation of split DNAzyme. The proximity ligation of split DNAzyme forms an intact DNAzyme to identify the loop section in the L probe and generates a nicking site to release the loop sequence ("3" and "4" fragments). The "3" and "4" fragments forms an intact sequence to induce the catalytic hairpin assembly, exposing the G-rich section. The released the G-rich sequence of LR probe induces the formation of G-quadruplex-hemin DNAzyme as a colorimetric signal readout. The absorption intensity demonstrated a strong linear association with the logarithm of the S. aureus concentration across a wide range of 5 orders of magnitude dynamic range under the optimized experimental parameters. The limit of detection was calculated to be 23 CFU/ml and the method showed high selectivity for MRSA.

• Journal of Microbiology and Biotechnology
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• 제34권3호
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• pp.681-688
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• 2024

The accurate and rapid detection of methicillin-resistance of Staphylococcus aureus (SA) holds significant clinical importance. However, the methicillin-resistance detection strategies commonly require complicated cell lysis and gene extraction. Herein, we devised a novel colorimetric approach for the sensitive and accurate identification of methicillin-resistance of SA by combining allosteric probe-based target recognition with self-primer elongation-based target recycling. The PBP2a aptamer in the allosteric probe successfully identified the target MRSA, leading to the initiation of self-primer elongation based-cascade signal amplification. The peroxidase-like hemin/G-quadruplex undergo an isothermal autonomous process that effectively catalyzes the oxidation of ABTS2- and produces a distinct blue color, enabling the visual identification of MRSA at low concentrations. The method offers a shorter duration for bacteria cultivation compared to traditional susceptibility testing methods, as well as simplified manual procedures for gene analysis. The overall amplification time for this test is 60 min, and it has a detection limit of 3 CFU/ml. In addition, the approach has exceptional selectivity and reproducibility, demonstrating commendable performance when tested with real samples. Due to its advantages, this colorimetric assay exhibits considerable potential for integration into a sensor kit, thereby offering a viable and convenient alternative for the prompt and on-site detection of MRSA in patients with skin and soft tissue infections.

• 대한인간공학회지
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• 제19권1호
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• pp.63-76
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• 2000

In this study, the factors affecting reality aspect of virtual reality system were identified through Quality Function Deployment. The influence of some selected factors was evaluated in simulated performance test. It was shown that the factor of whether user can break through the objects in virtual space is the most sensitive to performance, followed by the factors of rotation angle and movement step. Also, the results implied that as more referents were provided to user, feeling of reality was enhanced.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 추계학술대회 논문집 전기기기 및 에너지변환시스템부문
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• pp.18-20
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• 2003

In this paper, design and performance analysis of switched reluctance motor(SRM) according to design parameters are foaled. The parameters which are sensitive to the performance are examined and selected to have a good performances. Prototype machines are constructed to compare with the simulated results.

• 대한기계학회논문집B
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• 제27권11호
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• pp.1572-1578
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• 2003

Particle Image Thermometry(PIT) with liquid crystal tracers is used for visualizing and analysis of the bubbly flow in a vertical temperature gradient. Quantitative data of the temperature were obtained by applying the color-image processing to a visualized image, and neural-network was applied to the color-to-temperature calibration. This paper describes the method, and presents the transient mixing temperature patterns of the bubbly flow.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1986년도 한국자동제어학술회의논문집; 한국과학기술대학, 충남; 17-18 Oct. 1986
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• pp.637-640
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• 1986

The design and use of a micro-computer-based system for quantitative study of ocular kinetics are described. An algorithm for microcomputer analysis of electro-oculographically recorded saccadic eye movement is presented. From a brief, 4-min recording session detailed statistical information about saccade amplitude, duration, and velocity can be obtained. Since this data is not significantly altered by practice of motivational factors, it provides a sensitive functional test of the oculomotor system and its brain control system.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2004년도 춘계학술대회 논문집 전기기기 및 에너지변환시스템부문
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• pp.58-61
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• 2004

In this paper, design and performance analysis of switched reluctance motor(SRM) according to design parameters are researched. The parameters which are sensitive to the performance are examined and selected to have good performances. Some effective guide lines to have a good performance motor are suggested. Prototype machines are constructed to compare with the simulated and tested results.

• Journal of Pharmaceutical Investigation
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• 제15권3호
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• pp.151-159
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• 1985

For the determination of aliphatic carbamates, the quantitative analysis of carisoprodol was investigated by gab chromatography (GC) and spectrophotometry. All the methods studied were found to be very quantitative. The minimum experimental amounts of GC method, spectrophotometric method I and II were approximately $10^{-9},\;10^{-5}$ and $10^{-8}$ mole, respectively. The obtained results showed that GC method I was much more sensitive and rapid than spectrophotometric method II.

• 한국환경농학회지
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• 제12권3호
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• pp.298-308
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• 1993

Immunochemical assay, ELISA for small molecules such as pesticides are rapid, sensitive, cost effective and can easily analyze with large samples. ELISA is one of several powerful biotechnologies immediately applicable to pesticide analysis. This review lists the advantages and disadvantages of the ELISA and elucidate the steps in assay development using examples from this laboratory. The focus is primarily on hapten synthesis strategies, protein conjugation, Immunization, assay format, and assay validation.

• Asian Pacific Journal of Cancer Prevention
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• 제15권11호
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• pp.4377-4384
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• 2014

According to the World Health Organization (WHO), 1.37 million people died of lung cancer all around the world in 2008, occupying the first place in all cancer-related deaths. However, this number might be decreased if patients were detected earlier and treated appropriately. Unfortunately, traditional imaging techniques are not sufficiently satisfactory for early detection of lung cancer because of limitations. As one alternative, breath volatile organic compounds (VOCs) may reflect the biochemical status of the body and provide clues to some diseases including lung cancer at early stage. Early detection of lung cancer based on breath analysis is becoming more and more valued because it is non-invasive, sensitive, inexpensive and simple. In this review article, we analyze the limitations of traditional imaging techniques in the early detection of lung cancer, illustrate possible mechanisms of the production of VOCs in cancerous cells, present evidence that supports the detection of such disease using breath analysis, and summarize the advances in the study of E-noses based on gas sensitive sensors. In conclusion, the analysis of breath VOCs is a better choice for the early detection of lung cancer compared to imaging techniques. We recommend a more comprehensive technique that integrates the analysis of VOCs and non-VOCs in breath. In addition, VOCs in urine may also be a trend in research on the early detection of lung cancer.