• Molecules and Cells
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• 제26권2호
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• pp.146-151
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• 2008

The brown planthopper (BPH) is a major insect pest in rice, and damages these plants by sucking phloem-sap and transmitting viral diseases. Many BPH resistance genes have been identified in indica varieties and wild rice accessions, but none has yet been cloned. In the present study we report fine mapping of the region containing the Bph1 locus, which enabled us to perform marker-aided selection (MAS). We used 273 F8 recombinant inbred lines (RILs) derived from a cross between Cheongcheongbyeo, an indica type variety harboring Bph1 from Mudgo, and Hwayeongbyeo, a BPH susceptible japonica variety. By random amplification of polymorphic DNA (RAPD) analysis using 656 random 10-mer primers, three RAPD markers (OPH09, OPA10 and OPA15) linked to Bph1 were identified and converted to SCAR (sequence characterized amplified region) markers. These markers were found to be contained in two BAC clones derived from chromosome 12: OPH09 on OSJNBa0011B18, and both OPA10 and OPA15 on OSJNBa0040E10. By sequence analysis of ten additional BAC clones evenly distributed between OSJNBa0011B18 and OSJNBa0040E10, we developed 15 STS markers. Of these, pBPH4 and pBPH14 flanked Bph1 at distances of 0.2 cM and 0.8 cM, respectively. The STS markers pBPH9, pBPH19, pBPH20, and pBPH21 co-segregated with Bph1. These markers were shown to be very useful for marker-assisted selection (MAS) in breeding populations of 32 F6 RILs from a cross between Andabyeo and IR71190, and 32 F5 RILs from a cross between Andabyeo and Suwon452.

• 농촌의학ㆍ지역보건
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• 제48권4호
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• pp.239-250
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• 2023

이 연구는 농업인의 업무상질환에 대한 예방사업을 위한 사전 연구로서 업무상질환 등 농업인들이 인지하고 있는 우선순위를 선정하고, 또한 예방사업 역시 농업인들이 인지하고 있는 주요질환들의 사업 중 효과와 실천가능성을 파악하여 이후 농업인 안전보건사업의 근거자료로 활용하고자 한다. 연구 대상자는 지역에 거주하고 있는 농업인들을 눈덩이 모집 방식으로 선정하였으며 전체 671명을 대상으로 하였다. 우선순위 선정 방식은 BPRS 방법으로 하였으며, 각 업무상질환 중근골격질환, 심혈관 및 호흡기질환 그리고 농약 중독 예방을 위한 세부 사업에 대한 농업인들인 인지하는 효과와 실천가능성을 설문 조사하였다. 업무상질환 중 가장 높은 우선순위는 근골격질환이었으며, 호흡기질환, 농약 중독 등의 순이었으며, 근골격계질환의 세부 사업 중 '농작업편이장비, 보조도구 활용'이 인지하고 있는 효과정도와 실천가능성이 가장 높았다. 농약 중독 5가지 세부 사업 중 효과는 '농약 방제복/장갑 등 보호장비 구비'가 67.4%로 가장 높았으며, 실천가능성 정도는 '농약 사용법 준수'가 64.3%로 가장 높았다. 호흡기질환 예방을 위한 4가지 세부사업 중 효과와 실천 가능성 모두 '분진마스크 또는 방독마스크 착용'이 65.5%로 가장 높았다. 농업인들을 위한 안전보건사업을 수행할 때 근골격질환을 위한 사업을 우선적으로 하여야 할 것이며, 농약중독, 호흡기질환 역시 우선순위를 고려하여야 하며, 농업인들이 인지하고 있는 효과 크기와 실천 가능성을 함께 고려하여 사업 내용을 구성하여야 할 것이다.

• 한국어병학회지
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• 제5권1호
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• pp.37-48
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• 1992

항생제란 어떤 미생물의 생성물질로서 다른 미생물의 증식을 억제하거나 살균작용을 나타내는 것을 의미한다. 현재까지 알려진 4000여종의 항생제중, 수산용으로는 약 20여종이 사용되고 있다. 이러한 항생제의 분류는 화학적 구조와 부수되는 기능에 의하는 것이 일반적이며 그 효과는 적용되는 병원성균의 종류와 어류가 처해있는 환경에 따라 대단히 영향을 받는다. 그러므로 항생제의 종류와 적용방법은 정확한 질병에 대한 원인 규명이 있은 후 결정되어져야 할 것이다. 무절제하게 사용된 항생제는 자연적으로 발생하여 존재하고 있는 내성균을 선택적으로 증식 가능케 한다. 이미 일본의 수많은 양어장에서 내성균 출현의 보고가 있었고, 많은 종류의 내성균은 R plasmid를 가지고 있다는 것도 밝혀졌다. 우리나라의 양어장에서도 내성균 출현과 R plasmid에 의한 내성균 확산은 곧 문제가 될 수 있는 가능성이 크므로 전반적인 역학조사가 있어야 할 것이다. 상용항생제의 어류에 대한 약동력학, 잔존량, 임상적용 실험등에 대한 연구는 상당히 되어 있어 적절한 이들 항생제의 사용이 가능하지만, 현재까지 대부분의 수산용 항생제에 대해서는 연구해야 될 부분이 아직 많이 남아 있다. 그러므로 어류질병 치료와 항생제의 정확한 사용을 위해서는 양식사업을 하고 있는 현장과 진단을 할 수 있는 실험실의 긴밀한 협조관계가 무엇보다 중요하다 할 것이다.

• 농약과학회지
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• 제2권2호
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• pp.83-89
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• 1998

사과 주산단지로부터 1995년부터 1997년까지 3개년에 걸쳐 저장시 부패를 일으키는 병 피해를 조사하고 분리된 병원균의 병원성을 검정한 결과, Alternaria spp., Botryosphaeria dothidea, Botrytis cinerea, Fusarium spp., and Penicillium spp. 등 10종의 진균이 분리되었으며, 그 중 Botrytis cinerea, Penicillium spp. 및 Fusarium은 분리빈도도 높았고 병원성도 높았다. 균사생장에 있어서 Penicillium spp.은 $10{\sim}30^{\circ}C$에서, Botrytis cinerea 및 Alternaria spp.는 $5{\sim}30^{\circ}C$의 범위에서 잘 되었다. Penicillium spp.와 Alternaria spp.는 pH와 관련없이 생장하는 것으로 나타났고 Botrytis cinerea는 pH가 증가함에 따라 생장이 감소되었다. 포자 형성에 있어서는 Penicillium spp.는 $15{\sim}25^{\circ}C$, Botrytis cinerea는 $10{\sim}20^{\circ}C$에서 양호하였다. 사과선과가 저장병 발생에 미치는 영향을 조사한 결과 선과를 소홀히 할 경우 피해가 큰 것으로 나타났다. 생육기중 건전과일을 채취하여 과피서식미생물을 조사한 결과 저장병에 관여하는 대부분의 진균이 분리된 점으로 보아 저장병균은 포장에서 오염되어 저장기간 중에 병을 일으키는 것으로 판명되었다. 사과저장병해를 줄이기 위하여 프로라츠 유제등 사과병해방제용 약제를 수확전 30일에 살포한 결과 저장병 피해를 현저히 줄일 수 있었다.

• 한국육종학회지
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• 제43권5호
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• pp.413-421
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• 2011

본 연구에서는 관행의 교배육종과 DNA 마커를 이용한 MAS의 접목을 통하여 벼멸구 저항성과 관련된 단점을 보완하고, 효율적으로 도열병, 줄무늬잎마름병, 흰잎마름병, 벼멸구, 끝동매미충 저항성이 집적된 복합내병충성 우량계통을 육성하고자 수행하였다. 교배모본으로는 완전미율이 높고, 끝동매미충에 저항성인 '남평'과 단간이면서 흰잎마름병에 저항성인 '주남'을 반복친으로 사용하였고, 벼멸구저항성 유전자 Bph1을 가지고 있지만 간장이 크고 재배안전성이 미흡한 '밀양220호'를 공여친으로 사용하였다. 벼멸구 저항성 연관마커 RM28493을 이용하여 $BC_1F_1$ 및 $BC_2F_1$ 세대에서 MAS로 저항성 개체 선발과 여교잡을 수행하였으며, 복교잡 $F_1$ 세대에서 MAS를 통한 벼멸구 저항성이 고정된 계통을 선발하였다. 고세대 계통에서 생물검정을 통하여 흰잎마름병, 끝동매미충에 저항성인 계통을 선발하였다. MAS와 생물검정을 통해 도열병, 흰잎마름병, 줄무늬잎마름병, 벼멸구 및 끝동매미충에 저항성인 85개의 복합내병충성계통을 선발하였다. 복합내병충성 계통의 간장, 완전미율 및 수량은 각각 71~88 cm, 51~93%, 449~629 kg/10a 사이에 분포하였다. 복합내충성계통중 간장, 완전미율 및 수량이 남평벼와 비슷한 계통을 선발하여 '밀양265호'로 명명하였다. 최종적으로 본연구에서는 기존의 교배육종과 분자육종의 접목을 통하여 복합내병충성 우량계통을 육성하였으며, 이는 금후 유용교배모본으로 활용될 것으로 기대된다.

• 대한한의학원전학회지
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• 제20권2호
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• pp.175-193
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• 2007

A theory of the school which attach great importance to moxibustion therapy was more developed from 'Ge Hong(葛洪)', 'Wang Tao(王燾)' up to "BianQueXinShu(扁鵲心書)" of 'Dou Cai(竇材)' in Song Dynasty. The first volume of "BianQueXinShu" was described about the principles of health preserving method, diagnosis, treatment related with meridian system, support Yang theory, moxibustion therapy over the 10 chapters and in the continued 3 chapters, explained the symptoms and related moxibustion therapies. The summary is as follows. The Yang energy is the essence of the human body and it is minutely explained in "Hwangdineijing(黃帝內經)". However, the younger scholars after 'Zhang Zhongjing(張仲景)' held different views with "Hwangdineijing" so they didn't control serious diseases. Supporting the Yang energy, it will be possible to human body in good health and long life and perennial youth and longevity. To do like this, the first important thing is a moxibustion, the second is a Taoist hermit medication(丹藥) and the third is well usage of Radix Aconiti Lateralis Preparata. According to the sequence of Yang energy deficiency, the stages of diseases are classified as Ordinary Gi(平氣), Latter deficient state(微虛), The more deficient state(甚虛), Exhausting state(將脫), Exhausted state(已脫) and in the consideration of each stage, it is used gradually with warm-natured berbs, warm-acrid herbs, warm-heated herbs and moxibustion therapy. If it comes to the stage of Exhausted state, the Yang energy is too weak to treat a disease. Therefore it is easy to harm human body with usage the treatment of the Purgationist school theory or the Cold and cool medical school theory, so it is needed a great attention to use these therapies. To summarized the keynote of 'Du Jae''s moxibustion therapy, the one is a minority of selection of points(1$^{\sim}$2 acupuncture points), the second is a majority of moxibustion units(50$^{\sim}$500 units), and the other is a focused selection of points on spleen and kidney(especially Gwanwon, CC4). And in this book, it was explained concretely about the size of moxibustion, according to the experiment with mentioned size, the burning time of moxibustion was almost 4 min 40 sec, so the big size moxibustion was one of the characteristic of moxibustion therapy revealed in this book. Also it was used 'Suseongsan(睡聖散)' - a kind of analgesic herb complexes - to reduce a pain during the usage of moxibustion therapy in this book. To develop the moxibustion theory, it is more investigated in the future that there wasn't significant relation between Gwanwon(CC4) and spleen and kidney meridian in theory, compared to many used Gwanwon(CC4) in the prescription, where as mentioned the importance of spleen and kidney in treatment, that considering the burning time(1 unit - 4 min 40 sec, 12 units an hour, maximum 288 units a day) there were no guidances about meals, sleeping, stool and urine, and that there was no concrete study about the toxicity of 'Suseongsan' as analgesic moxibustion therapy.

• Plant Biotechnology Reports
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• 제3권4호
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• pp.277-283
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• 2009

One of the limitations to conducting maize Agrobacterium-mediated transformation using explants of immature zygotic embryos routinely is the availability of the explants. To produce immature embryos routinely and continuously requires a well-equipped greenhouse and laborious artificial pollination. To overcome this limitation, an Agrobacterium-mediated transformation system using explants of type II embryogenic calli was developed. Once the type II embryogenic calli are produced, they can be subcultured and/or proliferated conveniently. The objectives of this study were to demonstrate a stable Agrobacterium-mediated transformation of maize using explants of type II embryonic calli and to evaluate the efficiency of the protocol in order to develop herbicide-resistant maize. The type II embryogenic calli were inoculated with Agrobacterium tumefaciens strain C58C1 carrying binary vector pTF102, and then were subsequently cultured on the following media: co-cultivation medium for 1 day, delay medium for 7 days, selection medium for $4{\times}14$ days, regeneration medium, and finally on germination medium. The T-DNA of the vector carried two cassettes (Ubi promoter-EPSPs ORF-nos and 35S promoter-bar ORF-nos). The EPSPs conferred resistance to glyphosate and bar conferred resistance to phosphinothricin. The confirmation of stable transformation and the efficiency of transformation was based on the resistance to phosphinothricin indicated by the growth of putative transgenic calli on selection medium amended with $4mg\;1^{-1}$ phosphinothricin, northern blot analysis of bar gene, and leaf painting assay for detection of bar gene-based herbicide resistance. Northern blot analysis and leaf painting assay confirmed the expression of bar transgenes in the $R_1$ generation. The average transformation efficiency was 0.60%. Based on northern blot analysis and leaf painting assay, line 31 was selected as an elite line of maize resistant to herbicide.

• 한국응급구조학회지
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• 제25권1호
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• pp.147-158
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• 2021

Purpose: This study was conducted as a direct investigation of the data in the dispatch logbooks and status of patient transportation provided by private emergency transport companies in Busan. Methods: This study was conducted using SPSS 23.0 version for a total of 1,000 processed records of private emergency ambulance services in Busan from September 23, 2017 to November 5, 2019. Results: First, 100% of the emergency patient transfers by private emergency ambulances were carried out between medical institutions; 76.4% of all transfer patients had emergency conditions, and 86.0% had serious diseases. Second, 59.3% of the emergency patients were located at distances less than 10 km and 43.2%, at more than 10 km from the medical institutions. Third, 63.5% of the passengers were accompanied by first-class emergency rescuers according to the severity of the condition. Fourth, 92.7% of the reasons for the selection of medical institutions were transferred to places where professional care was available, accounting for most of the reasons for the selection. Finally, the medical institutions were selected according to the severity of the patient's condition; 76.5% patients were transported to institutions with a large number of doctors, and 42.9% of those were transported to specialized care institutions. Conclusion: This study collected data from 1,000 dispatch records of private emergency transport companies in Busan; these records reflect the government's policies to improve the emergency patient transfer system. The current status of emergency patient transfer offered by private transport companies was analyzed. All of the emergency patient transfers were carried out between medical institutions, and 76% of the transferred patients had emergency conditions.

• 한국환경보건학회지
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• 제45권2호
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• pp.134-141
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• 2019

Objectives: The study aimed to investigate the characteristics of health masks and to suggest the proper selection and use of protecting the respiratory system from particulate matters. Methods: The National Standard of masks promulgated by the Ministry of Food and Drug Safety (MFDS) and the Ministry of Employment and Labor (MOEL) were reviewed. The raw data of certified health masks were obtained from the MFDS database. Descriptive statistical analysis was performed. Results: Overall, 543 masks were certified by MFDS as of March 2019. Numbers of certified masks as KF80 (Korea Filter), KF94 and KF99 were 257 (47.3%), 281 (51.8%), and 5 (0.9%), respectively. Names of health masks, which were yellow sand, communicable diseases, health or its combination, were diverse that made customers be confused in selection. Health masks were also classified by particulate size, however, detailed information was not available. Conclusions: We investigated the status of health mask certification in Korea. Appropriate information on KF grade of health masks is required for customers to use them properly. MFDS should update the certification system of health masks periodically.

• Korean Journal of Acupuncture
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• 제19권1호
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• pp.107-130
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• 2002

In the clinics of traditional medicine, various acupuncture methods, that is Sa-Am' acupuncture, scalp acupuncture, auricular acupuncture and Dong-Shi' acupuncture, have been introduced and increased gradually to treat the diseases. In this bibliographic study, we examined the interrelation between the Dong Shi' acupuncture and 12 regular channels. The theoretical origination of Dong Shi' acupuncture and 12 regular channels was applied from Yin and Yang, Five Element, State of Viscera and so on. As the treatment using by 12 regular channels have used to 'regulating Qi and Treating Shin (調氣治神)', Dong Shi' acupuncture have used to the itself defense mechanism and relative balance of human beings. Also, these methods have the same rules that was selected to the opposing needling (巨刺) of remote point selection, however, Ah-Shi' points (阿是穴) were not used for the selection. Of Dong-Shi' acu-points, there were many ones which have the same location as the acupoints of regular channels. However, these one was renamed according to the new characteristics and therapeutics found by Dong Shi. Dong Shi' acupuncture has divided human body to the 12 areas but not considered to the concept of a mutual connection of human being body. The needling manipulation methods were vary at the regular channels, but Dong Shi' acupuncture did not use the traditional manipulation method except for supplementary Dong-Qi acupuncture, To-Ma acupuncture and To-In acupuncture. From these study, we found that the Dong-Shi' acupuncture have something in common with 12 regular channels, although the Dong-Shi' acupuncture was not based on the theory of 12 regular channels.