• Title/Summary/Keyword: Selection Effects

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Development of Disease-resistant Japonica Rice Varieties and Effects of Pyramiding Resistance Genes (내병성 자포니카 벼 계통 육성과 저항성 유전자 집적효과)

  • Kim, Woo-Jae;Baek, Man-Kee;Park, Hyeon-Su;Lee, Geon-Mi;Lee, Chang-Min;Kim, Seok-Man;Cho, Young-Chan;Seo, Jeong-Phil;Jeong, O-Young
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.314-326
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    • 2020
  • This study was carried out to develop a resistant variety against the K3a race of bacterial blight, Xanthomonas oryzae pv. oryzae, through expansion and pyramiding of resistance genes. To develop an elite bacterial blight-resistant cultivar, the breeding process and bacterial blight resistance reactions in advanced backcross lines (ABLs) were analyzed. ABLs21 which contain Xa3 and Xa21, were developed by double backcrossing japonica cultivar Hwanggeumnuri, which has bacterial blight resistant Xa3 gene, and indica variety IRBB21, which havs Xa21 gene, followed by disease resistance bioassay and marker-assisted selection. The resistance genes of ABLs21 were amplified by PCR with the molecular markers 9643.T4 (Xa3) and U1/I1 (Xa21). Hwanggeumnuri and IRBB3 showed resistance reactions against K1, K2, and K3 races, and a susceptible reaction against K3a, K4, and K5 races. IRBB21 showed resistance reactions against K2, K3, K3a, K4 and K5 races, and a susceptible reaction against K1 race. Hwanggeumnuri showed susceptible reactions at the seedling, tillering and adult stages (all stages), whereas ABL21-1 showed moderate resistance at the tillering stage. ABL21-1 showed stable resistance against 18 isolates of K3a race, and the lesion length was shorter than that of the donor parents. In cluster analysis, the HB4032 isolate showed the highest pathogenicity among the 18 isolates. The molecular marker polymorphisms and average substituted chromosome segment lengths of ABLs21 were 63.2 % and 86.1 cM, respectively. Insertion of the donor chromosomal segments occurred in the predicted region of the Xa21 gene of ABLs21.

Influence of Various Root Media in Pot Growth of 'Seolhyang' Strawberry on the Growth of Daughter Plants and Early Yield after Transplant ('설향' 딸기 포트육묘를 위한 혼합상토 종류가 자묘의 생육과 정식 후 초기수량에 미치는 영향)

  • Park, Gab Soon;Kim, Yeoung Chil;Ann, Seoung Won;Kang, Hee Kyoung;Choi, Jong Myung
    • Horticultural Science & Technology
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    • v.33 no.2
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    • pp.219-226
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    • 2015
  • The objective of this research was to investigate the influence of various root media on the growth of mother and daughter plants during propagation and early yield after transplanting of 'Seolhyang' strawberry. To achieve this, daughter plants were fixed to connected small pots that contained expanded rice-hull (ERH), a strawberry-specialized commercial medium (SSCM), soil mother materials (SMM), or loamy sand (LS). Then, growth of daughter plants in above- and below-ground tissue as well as early yield after transplanting to plastic house soil were investigated. The growth of daughter plants in terms of plant height, leaf area and fresh weight were the highest in the SSCM treatment. Root growth in terms of the amount of primary roots and root dry weight were the highest in the treatments of ERH and SMM and the lowest in that of SSCM, among treatments tested. The ERH treatment also showed the highest values among treatments in root length, surface area and volume when roots with 0 to 0.4 mm in diameter were investigated. The flower bud differentiation of daughter plants began on Sept. 3 in the ERH treatments, earlier than the SMM (Sept. 5) and in SSCM (Sept. 7) treatments. The tissue N contents of daughter plants were in the range of 1.41 to 1.55% in all treatments, and no significant differences were observed among treatments. This indicates that the low moisture retention capacity of ERH and water stress, rather than tissue N contents, promote the flower differentiation of daughter plants. In the evaluation of early yield after transplant, the ERH treatment of showed the highest yield in the period from November to December, reaching 667 g fruit weight per 10 plants. The yields per 10 plants in the other treatments were 581 g in SMM, 475 g in SSCM and 295 g in LS. Above results imply that the various root media have different effects on the growth of daughter plants as well as flower bud differentiation. Therefore, improvement in early yield after transplant can be achieved through selection of proper root medium for daughter plant propagation.

A Study on Enrollment Satisfaction, Career Directions, and Image of Dental Hygienists for Dental Hygiene Students in Some Regions (일부지역 치위생(학)과 학생들의 진학만족도와 진로방향, 치과위생사 이미지에 관한 연구)

  • Moon, Seon-Jeong;Ku, In-Young;Choi, Hwa-Young;Ka, Kyung-Hwan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.1
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    • pp.564-575
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    • 2015
  • A self-administered survey was conducted in dental hygiene students at colleges or universities in Daegu and North Gyeongsang Province to provide basic data necessary to suggest standards for the direction of career for dental hygiene students and construct desirable image of dental hygienists. The data were collected from February to May 2013; with the exception of 46 questionnaires containing insincere responses, 1,679 were finally analyzed using SPSS/Win 18.0, drawing the following conclusion: The freshmen showed higher levels of regret for selection and enrollment recommendation than the sophomores, juniors, or seniors and the sophomores, juniors, and seniors showed higher levels of willingness to change courses than the freshmen; thus, those in lower grades showed higher environment satisfaction. As for desired career, regardless of grades, they showed higher preference for being employed as a dental hygienist; as for desired paths to get a job, the freshmen were more likely to get professors' recommendations and the sophomores, juniors, and seniors showed higher preference for open recruitment. They showed higher preference for getting a job in a large city because of greater possibility of improvement. As for the desired period of employment, regardless of grades, they showed higher preference for employment before getting married and for reemployment after their children grew up; as for desired workplace, the freshmen showed higher preference for a dental clinic in a hospital, whereas the sophomores, juniors, and seniors showed higher preference for a dental hospital. As for the differences in image of dental hygienists by grades, the freshmen and sophomores showed higher levels of attitudes than the juniors; the freshmen and sophomores showed higher levels of beliefs than the juniors or seniors; and the seniors showed higher levels of values than the freshmen, sophomores, or juniors, and the sophomores and juniors showed higher levels of values than the freshmen; thus, students in lower grades showed higher levels of attitudes and beliefs and those in higher grades showed higher levels of values. Such factors of enrollment satisfaction as willingness to change courses and persuasion to make enrollment had significant effects on image of dental hygienists. Dental hygiene students need to make efforts to establish a job identity and get desirable image of dental hygienists and to make constant efforts to promote more accurate and positive image of dental hygienists through systematic and positive public relations.

Airborne Hyperspectral Imagery availability to estimate inland water quality parameter (수질 매개변수 추정에 있어서 항공 초분광영상의 가용성 고찰)

  • Kim, Tae-Woo;Shin, Han-Sup;Suh, Yong-Cheol
    • Korean Journal of Remote Sensing
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    • v.30 no.1
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    • pp.61-73
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    • 2014
  • This study reviewed an application of water quality estimation using an Airborne Hyperspectral Imagery (A-HSI) and tested a part of Han River water quality (especially suspended solid) estimation with available in-situ data. The estimation of water quality was processed two methods. One is using observation data as downwelling radiance to water surface and as scattering and reflectance into water body. Other is linear regression analysis with water quality in-situ measurement and upwelling data as at-sensor radiance (or reflectance). Both methods drive meaningful results of RS estimation. However it has more effects on the auxiliary dataset as water quality in-situ measurement and water body scattering measurement. The test processed a part of Han River located Paldang-dam downstream. We applied linear regression analysis with AISA eagle hyperspectral sensor data and water quality measurement in-situ data. The result of linear regression for a meaningful band combination shows $-24.847+0.013L_{560}$ as 560 nm in radiance (L) with 0.985 R-square. To comparison with Multispectral Imagery (MSI) case, we make simulated Landsat TM by spectral resampling. The regression using MSI shows -55.932 + 33.881 (TM1/TM3) as radiance with 0.968 R-square. Suspended Solid (SS) concentration was about 3.75 mg/l at in-situ data and estimated SS concentration by A-HIS was about 3.65 mg/l, and about 5.85mg/l with MSI with same location. It shows overestimation trends case of estimating using MSI. In order to upgrade value for practical use and to estimate more precisely, it needs that minimizing sun glint effect into whole image, constructing elaborate flight plan considering solar altitude angle, and making good pre-processing and calibration system. We found some limitations and restrictions such as precise atmospheric correction, sample count of water quality measurement, retrieve spectral bands into A-HSI, adequate linear regression model selection, and quantitative calibration/validation method through the literature review and test adopted general methods.

Evaluation of Characteristics of Peanut Sprout Using Korean Cultivars (땅콩 품종을 이용한 싹나물 특성 평가)

  • Pae, Suk-Bok;Ha, Tae-Joung;Lee, Myoung-Hee;Hwang, Chung-Dong;Shim, Kang-Bo;Park, Chang-Hwan;Park, Keum-Yong;Baek, In-Yeol
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.4
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    • pp.394-399
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    • 2011
  • This experiment was conducted to select suitable cultivars and evaluate growth characteristics to get basic information for sprouting peanut. On sprouting peanut, it showed a rapid increase in trans-resveratrol content that has effects on anti-cancer, anti-inflammatory, blood-sugar-lowering and other beneficial cardiovascular in mouse. For this experiment, characteristics of peanut sprouts were tested in 37 cultivars grown for 7 days at $26^{\circ}C$ temperature. There were a lots of variations in the growth characteristics among cultivars as followers : The range of 100 grain weight was 56 to 142 g, hypocotyl length was 4.3 cm to 5.8 cm, diameter of hypocotyl was 5.0 to 8.0 mm, epicotyl length was 0.8 cm to 4.6 cm, seedling ratio per seed number was 84% to 100%, weight per seedling was 4.9 g to 8.4 g, the rate of hypocotyl cleavage was 0% to 46%, the content of trans-resveratrol was $22.5\;{\mu}g/g$ to $88.2\;{\mu}g/g$ and sprout yield was 360% to 820%. The selection points considered were high sprout yield, high seedling rate, high resveratrol content, low brownish cotyledon, no hypocotyl cleavage, and fat hypocotyl etc. The best cultivar selected was 'Jokwang' that showed 7.8 mm diameter, clean cotyledon color, 100% seedling rate, 0% hypocotyl cleavage, $63.3\;{\mu}g/g$ resveratrol, and 820% sprouting yield. This cultivar was expected to be of use as a new food and nutraceutical material. Relationship between growth characteristics showed that root length had significant positive correlations with epicotyl length, resveratrol content and sprouting yield but negative correlations with hypocotyl diameter and cleavage. Hundred grain weight showed negative correlations with resveratrol content, seedling rate and sprouting yield but positively correlated with curved hypocotyl rate and hypocotyl cleavage positively. This result showed small grain seed will be more appropriate for sprouting peanut.

Estimation of Genetic Parameters for Milk Production Traits in Holstein Dairy Cattle (홀스타인의 유생산형질에 대한 유전모수 추정)

  • Cho, Chungil;Cho, Kwanghyeon;Choy, Yunho;Choi, Jaekwan;Choi, Taejeong;Park, Byoungho;Lee, Seungsu
    • Journal of Animal Science and Technology
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    • v.55 no.1
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    • pp.7-11
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    • 2013
  • The purpose of this study was to estimate (co) variance components of three milk production traits for genetic evaluation using a multiple lactation model. Each of the first five lactations was treated as different traits. For the parameter estimation study, a data set was set up including lactations from cows calved from 2001 to 2009. The total number of raw lactation records in first to fifth parities reached 1,416,589. At least 10 cows were required for each contemporary group, herd-year-season effect. Sires with fewer than 10 daughters were discarded. Lactations with 305d milk yield exceeding 15,000 kg were removed. In total, 1,456 sires of cows were remained after all the selection steps. A complete pedigree consisting of 292,382 records was used for the study. A sire model containing herd-year-season, caving age, and sire additive genetic effects was applied to the selected lactation data and pedigree for estimating (co) variance components via VCE. Heritabilities and genetic or residual correlations were then derived from the (co) variance estimates using R package. Genetic correlations between lactations ranged from 0.76 to 0.98 for milk yield, 0.79~1.00 for fat yield, 0.75~1.00 for protein yield. On individual lactation basis, relatively low heritability values were obtained 0.14~0.23, 0.13~0.20 and 0.14~0.19 for milk, fat, and protein yields, respectively. For the combined lactation heritability values were 0.29, 0.28, and 0.26 for milk, fat, and protein yields. The estimated parameters will be used in national genetic evaluations for production traits.

Case Report of Radiotherapy to a Breast Cancer Patient with a Pacemaker (인공심장박동기가 이식된 유방암환자의 방사선 치료에 대한 사례 보고)

  • Chae, Seung-Hoon;Park, Jang-Pil;Lee, Yang-Hoon;Yoo, Suk-Hyun;Seong, Won-Mo;Kim, Kyu-Bo
    • The Journal of Korean Society for Radiation Therapy
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    • v.24 no.2
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    • pp.197-203
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    • 2012
  • Purpose: In this study, we considerate our radiation therapy process for the breast cancer patient implanted a pacemaker applying the machine movement surgery, shielding, beam selection. Materials and Methods: We perform radiation therapy to a 54 years old, breast cancer patient implanted a pacemaker. The patient underwent a surgery to move the position of a pacemaker to right side breast after consultation with cardiology department. Prescribed dose was 5,040 cGy and daily dose 180 cGy for 28 fractions. The 10 MV photon energy, field size 0/$9.5{\times}20$ cm, half beam and opposing portal irradiation are used. To find out appropriate thickness of shielding board, we carried out an experiment using a solid water phantom ($30{\times}30{\times}7$ cm), a Farmer-type chamber (TN30013, PTW, Germany) and a shielding board (Pb $28{\times}27{\times}0.1$ cm). We calculated expected absorbed dose to te pacemaker with absorb ratio and shielding ratio. In the PTP system (Eclipse, Varian, USA), we figured out how much radiation would be absorbed to the machine with and without shielding. First day of the radiation therapy, we measured head scatter to the pacemaker with MOSFET Dose Verification System (TN-RD-70-W, Medical Canada Ltd., Canada). Results: In the phantom measurement, we found out appropriate thickness was 2 mm of shielding board. In the RTP, when using 2 mm shielding the pacemaker will be absorbed 11.5~38.2 cGy and DVH is 77.3 cGy. In the first day of the therapy, 4.3 cGy was measured so 120.4 cGy was calculated during total therapy. The patient was free from any side effects, and the machine also normally functioned. Conclusion: As the report of association which have public confidence became superannuated, there is lack of data about new machine. We believe that radiation therapy to thiese kind of patients could be done successfully with co-operation, patient-suitable planning, accurate QA, frequent in-vivo dosimetry and monitoring.

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The Effect of Photoneutron Dose in High Energy Radiotherapy (10 MV 이상 고에너지 치료 시 발생되는 광중성자의 영향)

  • Park, Byoung Suk;Ahn, Jong Ho;Kwon, Dong Yeol;Seo, Jeong Min;Song, Ki Weon
    • The Journal of Korean Society for Radiation Therapy
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    • v.25 no.1
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    • pp.9-14
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    • 2013
  • Purpose: High-energy radiotherapy with 10 MV or higher develops photoneutron through photonuclear reaction. Photoneutron has higher radiation weighting factor than X-ray, thus low dose can greatly affect the human body. An accurate dosimetric calculation and consultation are needed. This study compared and analyzed the dose change of photoneutron in terms of space according to the size of photon beam energy and treatment methods. Materials and Methods: To measure the dose change of photoneutron by the size of photon beam energy, patients with the same therapy area were recruited and conventional plans with 10 MV and 15 MV were each made. To measure the difference between the two treatment methods, 10 MV conventional plan and 10 MV IMRT plan was made. A detector was placed at the point which was 100 cm away from the photon beam isocenter, which was placed in the center of $^3He$ proportional counter, and the photoneutron dose was measured. $^3He$ proportional counter was placed 50 cm longitudinally superior to and inferior to the couch with the central point as the standard to measure the dose change by position changes. A commercial program was used for dose change analysis. Results: The average integral dose by energy size was $220.27{\mu}Sv$ and $526.61{\mu}Sv$ in 10 MV and 15 MV conventional RT, respectively. The average dose increased 2.39 times in 15 MV conventional RT. The average photoneutron integral dose in conventional RT and IMRT with the same energy was $220.27{\mu}Sv$ and $308.27{\mu}Sv$ each; the dose in IMRT increased 1.40 times. The average photoneutron integral dose by measurement location resulted significantly higher in point 2 than 3 in conventional RT, 7.1% higher in 10 MV, and 3.0% higher in 15 MV. Conclusion: When high energy radiotherapy, it should consider energy selection, treatment method and patient position to reduce unnecessary dose by photoneutron. Also, the dose data of photoneutron needs to be systematized to find methods to apply computerization programs. This is considered to decrease secondary cancer probabilities and side effects due to radiation therapy and to minimize unnecessary dose for the patients.

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Separation of Nanomaterials Using Flow Field-Flow Fractionation (흐름 장-흐름 분획기를 이용한 나노물질의 분리)

  • Kim, Sung-Hee;Lee, Woo-Chun;Kim, Soon-Oh;Na, So-Young;Kim, Hyun-A;Lee, Byung-Tae;Lee, Byoung-Cheun;Eom, Ig-Chun
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.11
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    • pp.835-860
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    • 2013
  • Recently, the consumption of nanomaterials has been significantly increased in both industrial and commercial sectors, as a result of steady advancement in the nano-technologies. This ubiquitous use of nanomaterials has brought up the concern that their exposure to environments may cause detrimental effects on human health as well as natural ecosystems, and it is required to characterize their behavior in various environmental media and to evaluate their ecotoxicity. For the sake of accomplishing those assessments, the development of methods to effectively separate them from diverse media and to quantify their properties should be requisitely accompanied. Among a number of separation techniques developed so far, this study focuses on Field-Flow Fractionation (FFF) because of its strengths, such as relatively less disturbance of samples and simple pretreatment, and we review overseas and domestic literatures on the separation of nanomaterials using the FFF technique. In particular, researches with Flow Field-Flow Fractionation (FlFFF) are highlighted due to its most frequent application among FFF techniques. The basic principle of the FlFFF is briefly introduced and the studies conducted so far are classified and scrutinized based on the sort of target nanomaterials for the purpose of furnishing practical data and information for the researchers struggling in this field. The literature review suggests that the operational conditions, such as pretreatment, selection of membrane and carrier solution, and rate (velocity) of each flow, should be optimized in order to effectively separate them from various matrices using the FFF technique. Moreover, it seems to be a prerequisite to couple or hyphenate with several detectors and analyzers for quantification of their properties after their separation using the FFF. However, its application has been restricted regarding the types of target nanomaterials and environmental media. Furthermore, domestic literature data on both separation and characterization of nanomaterials are extremely limited. Taking into account the overwhelmingly increasing consumption of nanomaterials, the efforts for the area seem to be greatly urgent.

Selection of Trunk Injection Pesticides for Preventive of Pine Wilt Disease, Bursaphelenchus xylophilus on Japanese Black Pine (Pinus thunbergii) (곰솔(Pinus thunbergii)에서 소나무재선충병 예방을 위한 나무주사용 약제선발)

  • Lee, Sang-Myeong;Kim, Dong-Soo;Lee, Sang-Gil;Park, Nam-Chang;Lee, Dong-Woon
    • The Korean Journal of Pesticide Science
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    • v.13 no.4
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    • pp.267-274
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    • 2009
  • This study was carried out to select effective preventive pesticides against pine wilt disease caused by pinewood nematode (PWN), Bursaphelenchus xylophilus on trunk injection. 1,000 fold aquatic solution of abamectin 1.8% EC and emamectin benzoate 2.15% EC were lower mortality (7.3% and 8.3% respectively) against PWN on 1 day after treatment. However effects of abamectin 1.8% EC, emamectin benzoate 2.15% EC, fosthiazate 30% SL and fenitrothion 30% SL were inhibited the reproduction of PWN over 99.6% in Botrytis cineria media. Effect of trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC at the rate of $10\;m{\ell}$ per 10 cm in diameter of breast height (DBH) on mortality of Japanese black pine, Pinus thungergii by inoculated PWN was 0% and 3.3%, respectively at the applied year however when injection of fosthiazate 30% SL were treated with the rate of $5\;m{\ell}$ per 10 cm tree DBH, mortality of tree was 63.3%. Abamectin 1.8% EC and emamectin benzoate 2.15% EC was showed high preventive efficacy representing >90% against PWN at the following year. PWN preventing efficacy of trunk injection was lower in naturally occurred area (mortality of pine tree in control was 11.7% at the first year) of PWN than artificially infected site (mortality of pine tree in control was >76.7% at the first year), PWN preventing efficacy of trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC at the rate of $10\;m{\ell}$ per 10 cm in DBH was 91.5% and 82.9%, respectively, at the applied year and 89.5% and 82.6% respectively at the following year in PWN naturally occurred site. Control efficacy by trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC was more higher in 10 fold dilution with 10 fold high amount of aquatic solution than no dilution with 10 fold less amount of aquatic solution. The preventive effect of trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC at the rate of $5\;m{\ell}$ per 10 cm in DBH was showed 100% at the applied year in PWN inoculated tree.