• Fisheries and Aquatic Sciences
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• v.19 no.7
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• pp.31.1-31.6
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• 2016

Background: The objective of this study was to develop an efficient selectable marker for transgenic Dunaliella salina. Results: Tests of the sensitivity of D. salina to the antibiotic chloramphenicol and the herbicide Basta$^{(R)}$ showed that cells ($1.0{\times}10^6cells/ml$) treated with 1000 or $1500{\mu}g/ml$ chloramphenicol died in 8 or 6 days, respectively, whereas D. salina cells ($1.0{\times}10^6cells/ml$) treated with 5, 10, 20, or $40{\mu}g/ml$ Basta$^{(R)}$ died in 2 days. Therefore, D. salina is more sensitive to Basta$^{(R)}$ than to chloramphenicol. To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells and that the cells survived in $5{\mu}g/ml$ Basta$^{(R)}$, the medium used to select the transformed cells. Conclusions: The findings of this study suggest that the pat gene can be used as an efficient selectable marker when producing transgenic D. salina.

• Journal of Crop Science and Biotechnology
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• v.11 no.4
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• pp.233-236
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• 2008

A positive selectable marker system was adapted for transformation of mature embryo-derived calli of Indica rice (Oryza sativa L.) utilizing the PMI gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. The transformed cells grew on medium supplemented with 3% mannose as carbon source and calli were selected on media containing various concentrations of mannose. Molecular analyses showed that the transformed plants contained the PMI gene. The results indicate that the mannose selection system can be used for Agrobacterium-mediated transformation of mature embryo in rice to substitute the use of conventional selectable markers in genetic transformation.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.109-109
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• 2004

• Korean Journal of Plant Tissue Culture
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• v.22 no.4
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• pp.235-240
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• 1995

The development of selectable markers for transformation has been a major factor in the successful genetic manipulation of plant. We established a new selectable marker system for tobacco transformation using chimeric adenosine deaminase (ADA) gene, which confers resistance to cytotoxic adenosine analogues, 9-$\beta$-D-arabinofuranosyl adenine(Ara-A) and cordycepin. The transformants with the chimeric ADA gene in tobacco grew in the presence of normally lethal level of cytotoxic adenosine analogues, 100 $\mu$M Ara-A and 50 $\mu$M cordycepin. We successfully distinguished transformed shoot from non-transformed shoot on the same selectable media with cytotoxic adenosine analogues. In this selectable media, we were able to select seeds with/ without ADA gene from transgenic tobacco seeds. Theses results show that the mammalian ADA gene may serve as a new selectable marker for tobacco transformation.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.45 no.4
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• pp.92-99
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• 2008

We propose an effective voice-pishing detection algorithm based on the 3GPP2 selectable mode vocoder (SMV). The detection of voice pishing is performed based on a Gaussian mixture model (GMM) using decoding parameters of the SMV directly extracted from the decoding process of the transmitted speech information in the mobile phone. The experimental results indicate that SMV decoding parameters are effective in discriminating between general voice and phisher's voice and the performance is significantly acceptable when the proposed technique is applied.

• Current Optics and Photonics
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• v.3 no.3
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• pp.243-247
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• 2019

We have developed a compact, wavelength-selectable, Q-switched Nd:YAG laser at mid ultraviolet for chemical warfare agent detection. The fundamental wave at 1064 nm is delivered by a pulsed solid state laser incorporating with a square-type Nd:YAG rod in a resonator closed by two crossed Porro prisms for environmental reliability. The output energy at 213 nm ($5{\omega}$) and 266 nm ($4{\omega}$) by ${\chi}^{(2)}$ process in the sequentially disposed BBO crystals are measured to be 6.8 mJ and 15.1 mJ, respectively. The output wavelength is selected for $5{\omega}$ and $4{\omega}$ by a motorized wavelength switch. The energy ratio of the $5{\omega}$ to the $4{\omega}$ is varied from 0.05 to 0.85 by controlling the phase matching temperature of the nonlinear crystal for sum-frequency generation without change of the output pulse parameters.

• Proceedings of the IEEK Conference
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• 1999.06a
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• pp.733-736
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• 1999

In this Paper, Bit selectable and Bi-directional Interface Port is described, which can communicate data with the peripheral devices. Specially A description of the asynchronous design method is given to remove the clock skew phenomenon and the output asynchronous control method which finds the optimal clock and controls all the enable signal of the output pins at the same time is presented. Using this technique interface ports have delay time of less-than 0.5㎱.

• Proceedings of the Optical Society of Korea Conference
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• 2003.07a
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• pp.96-97
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• 2003

We developed a wavelength selectable 8-channel WDM otpical transmitter. The module consisted of two 4-channel DFB-LD arrays monolithically integrated with a 1x4 Power combiner and an SOA. It operated at 1550 nm and 200 GHz spacing.

• Plant Biotechnology Reports
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• v.4 no.4
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• pp.293-301
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• 2010

The feasibility of two strategies for transgene pyramiding using Agrobacterium-mediated transformation was investigated to develop a transgenic potato (Solanum tuberosum L. cv. Iwa) with resistance to potato tuber moth (PTM) (Phthorimaea operculella (Zeller)). In the first approach, cry1Ac9 and cry9Aa2 genes were introduced simultaneously using a kanamycin (nptII) selectable marker gene. The second approach involved the sequential introduction (re-transformation) of a cry1Ac9 gene, using a hygromycin resistance (hpt) selectable marker gene, into an existing line transgenic for a cry9Aa2 gene and a kanamycin resistance (nptII) selectable marker gene. Multiplex polymerase chain reaction (PCR) confirmed the presence of the specific selectable marker gene and both cry genes in all regenerated lines. The relative steady-state level of the cry gene transcripts in leaves was quantified in all regenerated lines by real-time PCR analysis. Re-transformation proved to be a flexible approach to effectively pyramid genes for PTM resistance in potato, since it allowed the second gene to be added to a line that was previously identified as having a high level of resistance. Larval growth of PTM was significantly inhibited on excised greenhouse-grown leaves in all transgenic lines, although no lines expressing both cry genes exhibited any greater resistance to PTM larvae over that previously observed for the individual genes. It is anticipated that these lines will permit more durable resistance by delaying the opportunities for PTM adaptation to the individual cry genes.

• Journal of the Korea Society of Computer and Information
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• v.13 no.4
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• pp.87-95
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• 2008

In this paper, we developed a selectable observation Point test architecture and test procedure for clocked 4-bit synchronous counter circuit based on boundary scan architecture. To develope, we analyze the operation of Sample/Preload instruction on boundary scan architecture. The Sample/Preload instruction make Possible to snapshot of outputs of CUT(circuit under test) at the specific time. But the changes of output of CUT during normal operation are not possible to observe using Sample/Preload in typical scan architecture. We suggested a selectable observation point test architecture that allows to select output of CUT and to observe of the changes of selected output of CUT during normal operation. The suggested a selectable observation point test architecture and test procedure is simulated by Altera Max 10.0. The simulation results of 4-bit counter shows the accurate operation and effectiveness of the proposed test architecture and procedure.