• 한국토양비료학회지
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• 제50권2호
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• pp.71-80
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• 2017

This study was conducted to investigate the application effect of Lysobacter antibioticus HS124 (gelatinase and chitinase producing microorganism; GCM) for the improvement of soybean yield and control of stink bug. Our results showed that the yield of GCM-treated soybean increased by 17.0, 20.3, 19.0, and 25.6% in the experimental field sites of Muan-gun, Sunchang-gun, Gwangju 1, and Gwangju 2, respectively, compared to the yield of the soybean obtained by conventional practice treatment (CPT); however in the Gimjae-si, the yield decreased by 10.6%. Results in both pods and seeds $plant^{-1}$ were significantly increased in Gwangju 2 by the GCM cultured broth treatment (GCMT). Ratio of 3 seeds $pod^{-1}$ in Sunchang-gun was statistically significant between GCMT and CPT, however, the result in the other field showed no significance. Germination rate was only statistically improved by GCMT in Gwangju 2 field site. GCMT reduced the appearance of stink bug in all experimental field sites except in Gimjae-si. The soybean seed damage by stink bug was no significance in all of treatments. Therefore, GCMT could improve the productivity of soybean and also control the infestation of stink bug.

• BMB Reports
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• 제38권6호
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• pp.650-660
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• 2005

Proteins accumulated in dry, stratified Arabidopsis seeds or young seedlings, totaled 1100 to 1300 depending on the time of sampling, were analyzed by using immobilized pH gradient 2-DE gel electrophoresis. The molecular identities of 437 polypeptides, encoded by 355 independent genes, were determined by MALDI-TOF or TOF-TOF mass spectrometry. In the sum, 293 were present at all stages and 95 were accumulated during the time of radicle protrusion while another 18 appeared in later stages. Further analysis showed that 226 of the identified polypeptides could be located in different metabolic pathways. Proteins involved in carbohydrate, energy and amino acid metabolism constituted to about 1/4, and those involved in metabolism of vitamins and cofactors constituted for about 3% of the total signal intensity in gels prepared from 72 h seedlings. Enzymes related to genetic information processing increased very quickly during early imbibition and reached highest level around 30 h of germination.

• 한국작물학회지
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• 제20권
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• pp.115-121
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• 1975

맥후작 면화재배에서 숙기를 단축하고 적채면수량을 증가시키고자 본실험을 실시하였든바 그 결과를 요약하면 다음과 같다. 1) 맥후작 면화재배에서 10월상순 Ethrel를 산포하면 적채면화율이 무처리구의 38%에 비하여 2,000ppm처리구의 그것은 93%였으며 개서는 20일이나 앞당겨 졌다. 2) 수량에서는 무처리구에 비하여 Ethrel처리구가 15%∼38%까지 증수되었다. 3) 한편 섬유장과 섬유장력에 있어서는 처리구는 무처리구와 차이 없었으나 적채면과 목채면간에는 무처리구 및 Ethrel처리구에 있어서 각각 평균 1.3mm 정도 목채면이 더 짧았다. 4) 1삭중과 종자 100립중에서도 Ethrel처리로 인한 차이는 전혀 없었으나 적채면과 목채면간에서는 목채면이 훨씬 가벼웠다. 5) Ethrel로 처리된 적채면종자는 무처리의 그것에 비하여 발아가 촉진되었다. 그러나 목채면종자의 발아율은 철된것이나 안된것이나 바슷했다. 6) 이상의 결과로서 맥후작면화재배에 있어서 만숙장섬유품종을 도입하여도 Ethrel를 적용하면 적채면수량이 더욱 증가된다고 본다.

• Journal of Microbiology and Biotechnology
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• 제17권1호
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• pp.52-57
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• 2007

Growth promotion of wild plants by some plant growth-promoting rhizobacteria (PGPR) was examined in the microcosms composed of soils collected separately from a grass-covered site and a nongrass-covered site in a lakeside barren area at Lake Paro, Korea. After sowing the seeds of eight kinds of wild plants and inoculation of several strains of PGPR, the total bacterial number and microbial activity were measured during 5 months of study period, and the plant biomasses grown were compared at the end of the study. Acridine orange direct counts in the inoculated microcosms, $1.3-9.8{\times}10^9\;cells{\cdot}g\;soil^{-1}$ in the soil from the grass-covered area and $0.9-7.2{\times}10^9\;cells{\cdot}g\;soil^{-1}$ in the soil from the nongrass-covered site, were almost twice higher than those in the uninoculated microcosms. The number of Pseudomonas sp., well-known bacteria as PGPR, and the soil dehydrogenase activity were also higher in the inoculated soils than the uninoculated soils. The first germination of sowed seeds in the inoculated microcosm was 5 days earlier than the uninoculated microcosm. Average lengths of all plants grown during the study period were 26% and 29% longer in the inoculated microcosms starting with the grass-covered soil and the nongrass-covered soil, respectively, compared with those in the uninoculated microcosms. Dry weights of whole plants grown were 67-82% higher in the inoculated microcosms than the uninoculated microcosms. Microbial population and activity and growth promoting effect by PGPR were all higher in the soils collected from the grass-covered area than in the nongrass-covered area. The growth enhancement of wild plants seemed to occur by the activities of inoculated microorganisms, and this capability of PGPR may be utilized for rapid revegetation of some barren lands.

• 한국산림과학회지
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• 제28권1호
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• pp.36-42
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• 1975

밤나무 종자(種子)의 발아초기(發芽初期)에 유근(幼根)과 유경(幼莖)을 할절(割切), 단일종자(單一種子)로부터 여러쌍(雙)의 동일유전자형(同一遺傳子型)을 얻는 방법(方法)으로 본연구(本硏究) 결과(結果), 밤나무종자(種子)의 유근(幼根)과 유경(幼莖)을 일주(一週) 간격(間隔)으로 1-3회(回)로 나누어 할절(割切)하여 2-8개체(個體)의 동일유전자형(同一遺傳子型)을 만들었다. 그 생장율(生長率)은 90-100%이며, 일회(一回)의 할절묘(割切苗)는 수회(數回)의 할절묘(割切苗)보다 생존율 생장량 및 T/R율(率)이 불량(不良)하고, 단일종자(單一種子)에서 얻은 4개체(個體)까지는 정상묘(正常苗와) 별 차이가 없고, 외부형태(外部形態)도 완전(完全)한 원형(原形)을 회복하였다.

• Journal of Biosystems Engineering
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• 제42권3호
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• pp.227-233
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• 2017

Purpose: Morphological properties of soybean roots are important indicators of the vigor of the seed, which determines the survival rate of the seedlings grown. The current vigor test for soybean seeds is manual measurement with the human eye. This study describes an application of a machine vision technique for rapid measurement of soybean seed vigor to replace the time-consuming and labor-intensive conventional method. Methods: A CCD camera was used to obtain color images of seeds during germination. Image processing techniques were used to obtain root segmentation. The various morphological parameters, such as primary root length, total root length, total surface area, average diameter, and branching points of roots were calculated from a root skeleton image using a customized pixel-based image processing algorithm. Results: The measurement accuracy of the machine vision system ranged from 92.6% to 98.8%, with accuracies of 96.2% for primary root length and 96.4% for total root length, compared to manual measurement. The correlation coefficient for each measurement was 0.999 with a standard error of prediction of 1.16 mm for primary root length and 0.97 mm for total root length. Conclusions: The developed machine vision system showed good performance for the morphological measurement of soybean roots. This image analysis algorithm, combined with a simple color camera, can be used as an alternative to the conventional seed vigor test method.

• Journal of Crop Science and Biotechnology
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• 제10권4호
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• pp.249-256
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• 2007

A study was conducted to observe the variation and inheritance of agronomic traits and their interrelationship in mungbean. The objective of the study was to compare agronomic traits and hardseed percentage of 268 recombinant inbred lines(RILs) developed from the cross between wild Vigna subspecies sublobata "ACC 41" with the mungbean cultivar "Berken". The RIL population and their parents were evaluated under controlled conditions in a glass house at the University of Queensland, Brisbane, Australia. The results showed significant differences among the RILs and among the parents in all traits under study. Berken had a longer flowering date and a higher seed weight per plant, but less total leaf number and pod number per plant than ACC 41. A germination test between papers revealed that ACC 41 was 100% hard-seeded and did not germinate at all, while Berken germinated up to 100%. Their RILs distributed well between 0 to 100% hardseed. Upon scarification, all hardseed germinated within seven days. Narrowsense heritability estimates of total leave number, hardseedness, pod length, and pod width were highly heritable at 89.9, 98.9, 93.7, and 93.2%, respectively. The heritability of seed weight per plant and number of seeds per plant were lower at 63.1 and 58.4%, respectively. Seed weight per plant showed positive transgressive segregation when compared with ACC 41 and a positive correlation with 100 seed weight. While the number of seeds per pod showed a negative transgressive segregation when compared with Berken and a negative correlation with pod length and pod width. The RILs gave a 1:1 segregation ratio in leaflet shape, growth habit, and growth pattern, indicating that these traits were controlled by a single dominant gene.

• Applied Biological Chemistry
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• 제41권7호
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• pp.522-526
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• 1998

콩 유용성분 탐색의 일환으로 그리고 향후 콩 ferritin 항체 및 유전자 확보를 목표로 발아된 콩으로부터 ferritin을 분리 정제하여 그 몇가지 특성을 조사하였다. 72시간 발아된 콩으로부터 ammonium sulfate 침전(0.55 saturation), DEAE-cellulose, Sephacryl S-300, Bio-Scale Q2 column chromatographies를 통하여 ferritin을 분리하였다. 정제된 콩 ferritin은 SDS-PAGE 분석에서 21 kDa의 크기를 나타냈으며, Sephacry S-300을 통한 겔거르기 chromatography와 non-denaturing 폴리아크릴아마이드 전기영동 분석에서 $510{\sim}560\;kDa$의 크기로 측정 되었다. 또한, immunodiffusion test에서 anti-soybean ferritin antiserum과 상호 반응하였다. 원자흡광광도계와 표준 철 용액을 이용한 정제된 콩 ferritin 중의 철 함유량은 833 mol Fe/mol protein 이었으며, 이는 호박씨로부터 분리한 ferritin보다 31배 더 많은 양의 철 함유량 이었다. 정제된 콩 ferritin중의 철은 horse spleen ferritin 중의 철과 유사하게 iron staining 되었다.

• 한국식물병리학회지
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• 제3권1호
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• pp.8-12
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• 1987

경기도 농촌진흥원에서 분양받은 녹두종자와 녹두 시험포의 뿌리 및 재배토양에서 Fusarium oxysporum, F. monitiforme, F. solani, F. equiseti, F. semitectum 및 F. sporotrichioides가 검출되었다. 종자를 대형 Petridish에서 발아시킨 유묘검정에서는 $7\%$, 자엽 및 배에서도 $2\%$의 감염율을 나타냈다. 녹두 종자에서 분리한 F. moniliforme, F. semitectum, F. equiseti 및 F. sporotrichioides를 Water-agar에서 병원성을 검정한 결과 모든 균주에서 병원성이 인정되었다. 이병근에서 F. oxysporum과 F. solani가 분리되었고, 이들의 병원성을 검정한 결과 Water-agar에서는 병원성이 인정되었으나 병원균접종토양에서는 약하게 나타났다. 재배토양에서는 F. oxysporum, F. solani 및 F. equiseti가 분리되었는데 이들도 Water-agar에서 병원성이 인정되었다. 병원균접종토양에서는 F. oxysporum과 F. equiseti는 병원성이 인정되었으나 F. solani는 병원성이 약하게 나타났다.

• 식물조직배양학회지
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• 제25권3호
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• pp.183-187
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• 1998

한란(Cymbidium kanran)의 근경에 돌연변이 물질인 EMS를 처리하여 엽록소변이 식물체를 유도하였다. 한란종자를 발아시켜서 근경을 유도할 때 종자에 40분간 초음파 처리한 것이 무처리구에 비하여 발아율이 5.5배 정도 높았다. EMS 0.2%를 포함한 근경증식용 액체배지에서 3주간 배양하였을 경우에 50-60%정도의 근경이 갈변되었다. 갈변된 근경을 근경증식용 고체배지에서 배양하였을 경우에 근경조직의 일부분으로부터 새로운 근경의 증식의 관찰되었고, 이러한 근경조직을 절단하여 1년간 계단배양하던중에 분열조직의 일부분에서 색소변이 근경이 관찰되었다. 색소변이 근경은 나선형의 줄무늬가 가장 많았고, 그 외 엷은 노란색과 흰색의 근경이 분리되었다. 나선형의 근경에서 유도된 식물체는 잎에 무늬가 전혀 없는 녹색의 식물체 또는 줄무늬가 들어 있는 식물체가 유도되었다. 그러나 엷은 노란색 근경에서 유도된 식물체는 노란색 줄무늬가 있는 식물체로, 흰색 근경으로부터 유도된 식물체는 백화된 식물체로만 분화하였다. 이상의 결과 한란근경을 이용한 색소변이식물체의 유도에 있어서 배양중인 근경에 EMS처리가 효과적인 것을 보여주었다. 이러한 방법을 다른 식물에 적용하면 색소변이 식물체를 개발할 수 있으리라 생각된다.