DOI QR코드

DOI QR Code

Effect of Gelatinase and Chitinase Producing Microorganism on the Growth of Soybean and Control of Stink Bug in Field

  • Lee, Yong-Seong (Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Jeon, Hyeon-Deok (Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Kim, Yun-Tae (Future Agricultural Strategy Institute) ;
  • Monkhung, Sararat (Crop Production Technology Program, Faculty of Animal Science and Agricultural Technology, Silpakorn University, Phetchaburi Information Technology Campus) ;
  • Kim, Kil-Yong (Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University)
  • Received : 2016.06.07
  • Accepted : 2017.04.27
  • Published : 2017.04.30

Abstract

This study was conducted to investigate the application effect of Lysobacter antibioticus HS124 (gelatinase and chitinase producing microorganism; GCM) for the improvement of soybean yield and control of stink bug. Our results showed that the yield of GCM-treated soybean increased by 17.0, 20.3, 19.0, and 25.6% in the experimental field sites of Muan-gun, Sunchang-gun, Gwangju 1, and Gwangju 2, respectively, compared to the yield of the soybean obtained by conventional practice treatment (CPT); however in the Gimjae-si, the yield decreased by 10.6%. Results in both pods and seeds $plant^{-1}$ were significantly increased in Gwangju 2 by the GCM cultured broth treatment (GCMT). Ratio of 3 seeds $pod^{-1}$ in Sunchang-gun was statistically significant between GCMT and CPT, however, the result in the other field showed no significance. Germination rate was only statistically improved by GCMT in Gwangju 2 field site. GCMT reduced the appearance of stink bug in all experimental field sites except in Gimjae-si. The soybean seed damage by stink bug was no significance in all of treatments. Therefore, GCMT could improve the productivity of soybean and also control the infestation of stink bug.

Keywords

References

  1. De Weger, L.A., A.J. van der Bij, L.C. Dekkers, M. Simons, C.A. Wijffelman, and B.J.J. Lugtenberg. 1995. Colonization of the rhizosphere of crop plants by plant-beneficial pseudomonads. FEMS Microbiol. Ecol. 17:221-228. https://doi.org/10.1111/j.1574-6976.1995.tb00205.x
  2. Farag, M.A., C.M. Ryu, L.W. Sumner, and P.W. Pare. 2006. GC-MS SPME profiling of rhizobacterial volatiles reveals prospective inducers of growth promotion and induced systemic resistance in plants. Phytochemistry. 67:2262-2268. https://doi.org/10.1016/j.phytochem.2006.07.021
  3. Frankenberger, W.T.Jr. and M. Arshad. 1995. Phytohormones in Soils. Microbial production and function, USA.
  4. Gerhardson, B. 2002. Biological substitutes for pesticides. Trends Biotechnol. 20:338-343. https://doi.org/10.1016/S0167-7799(02)02021-8
  5. Glick, B. 1995. The enhancement of plant growth by free-living bacteria. Can. J. Microbiol. 41:109-117. https://doi.org/10.1139/m95-015
  6. Ha, K.S., N.K. Heo, J.R. Kim, and S.H. Song. 1998. Effect of different seeding times and soybean varieties on damages and occurrence of hemiptera insects. RDA. J. Crop Prot. 40:32-36.
  7. Han, S.C. and K.M. Choi. 1988. Control and major insect pests on soybean in Korea. RDA Symposium. 3:153-165.
  8. Hong, S.J., Y.K. Kim, H.J. Jee, C.K. Shim, M.J. Kim, J.H. Park, E.J. Han, and B.C. Lee. 2011. Influence of disease severity of bacterial pustule caused by Xanthomonas axonopodis pv. glycines on soybean yield. Res. Plant Dis. 17(3):317-325. https://doi.org/10.5423/RPD.2011.17.3.317
  9. Jung, H.K., J.R. Kim, S.M. Woo, and S.D. Kim. 2006. An auxin producing plant growth promoting rhizobacterium Bacillus subtilis AH18 which has siderophore-producing biocontrol activity. Korean. J. Microbiol. Biotechnol. 34:94-100.
  10. Jung, H.K., J.R. Kim, S.M. Woo, and S.D. Kim. 2007. Selection of the auxin, siderophore, and cellulase-producing PGPR, Bacillus licheniformis K11 and its plant growth promoting mechanisms. J. Korean Soc. Appl. Biol. Chem. 50:23-28.
  11. Kang, C.H., H.S. Huh, and C.G. Park. 2003. Review on true bugs infesting tree fruits upland crops and weeds in Korea. Korean J. Appl. Entomol. 42:269-277.
  12. Kang, S.J., Y.S. Lee, S.Y. Lee, G.Y. Yun, S.H. Hong, Y.S. Park, I.S. Kim, R.D. Park, and K.Y. Kim. 2010. Biological control of diamondback moth (Plutella xylostella L.) by Lysobacter antibioticus HS124. Korean J. Soil Sci. Fert. 43:537-544.
  13. Khare, D. 2011. Expression of four-seeded pod in soybean. Curr. Sci. 101(12): 1535-1537.
  14. Kim, H.J., J.Y. Oh, D.K. Kim, H.T. Yun, W.S. Jung, J.K. Hong, and K.D. Kim. 2010. Evaluation of disease occurrence by cultivar, sowing date and locational difference in korean soybean fields. Res. Plant Dis. 16(2):176-182. https://doi.org/10.5423/RPD.2010.16.2.176
  15. Kim, J.T., S.Y. Park, W.B. Choi, Y.H. Lee, and H.T. Kim. 2008. Characterization of Colletotrichum isolates causing anthracnose of pepper in Korea. Plant Pathol. J. 24:17-23. https://doi.org/10.5423/PPJ.2008.24.1.017
  16. Kim, Y.S., M.S. Lee, J.H. Yeom, J.G. Song, I.K. Lee, and B.S. Yun. 2011. Screening of multifunctional bacteria with biocontrol and biofertilizing effects. Korean. J. Mycol. 39(2):126-130. https://doi.org/10.4489/KJM.2010.39.2.126
  17. Kloepper, J.W., J. Leong, M. Teintze, and M.N. Schroth. 1980. Pseudomonas siderophores: a mechanism explaining disease-suppressive soils. Curr. Microbiol. 4:317-320. https://doi.org/10.1007/BF02602840
  18. Ko, H.S., H. Tindwa, R.D. Jin, Y.S. Lee, S.H. Hong, H.N. Hyun, Y. Nam, and K.Y. Kim. 2011. Investigation of siderophore production and antifungal activity against Phytophthora capsici as related to Iron (III) nutrition by Lysobacter antibioticus HS124. Korean J. Soil Sci. Fert. 44(4):650-656. https://doi.org/10.7745/KJSSF.2011.44.4.650
  19. Ko, H.S., R.D. Jin, H.B. Krishnan, S.B. Lee, and K.Y. Kim. 2009. Biocontrol ability of Lysobacter antibioticus HS124 against Phytophthora Blight is mediated by the production of 4-hydroxyphenylacetic acid and several lytic enzymes. Curr. Microbiol. 59:608-615. https://doi.org/10.1007/s00284-009-9481-0
  20. Lee S.Y., S.B. Lee, Y.K. Kim, and H.G. Kim. 2004. Effect of agrochemicals on mycelial growth and spore germination of a hyperparasite, Ampelomyces quisqualis 94013 for controlling cucumber powdery mildew. Korean. J. Pestic. Sci. 8:71-78.
  21. Lee, M.W. 1997. Root colonization by beneficial Pseudomonas spp. and bioassay of suppression of Fusarium wilt of radish. Korean. J. Mycol. 25:10-19.
  22. Lee, S.G., J.U. Yoo, C.Y. Hwang, B.R. Choi, and J.O. Lee. 1997. Effect of temperature on the development of the bean bug, Riptortus clavatus Thunberg (Hemiptera: Alydidae). RDA. J. Crop Protec. 39(1):25-27.
  23. Lee, Y.S., M. Anees, H.N. Hyun, and K.Y. Kim. 2013. Biocontrol potential of Lysobacter antibioticus HS124 against the root-knot nematode, Meloidogyne incognita, causing disease in tomato. Nematology. 15:545-555. https://doi.org/10.1163/15685411-00002700
  24. Lenssen, A.W. 2013. Biofield and fungicide seed treatment influences on soybean productivity, seed quality and weed community. Agricu. J. 8(3):138-143.
  25. Lim, T.H. 2005. Antifungal activity of Streptomyces griseofuscus 200401 against pathogens causing late blight and anthracnose on pepper. Korean J. Pestic. Sci. 9:102-107.
  26. Malik, K.A., R. Bilal, S. Mehnaz, G. Rasul, M.S. Mirza, and S. Ali. 1997. Association of nitrogen-fixing, plant-growth-promoting rhizobacteria (PGPR) with kallar grass and rice. Plant Soil. 194:37-44. https://doi.org/10.1023/A:1004295714181
  27. Milosevic, M., J. Rainpreht, and P. Dokic, 1992. Effect of different seed size fractions on germination in sugar beet (Beta vulgaris L). Seed Sci. Technol. 20:703-710.
  28. Paik, C.H., G.H. Lee, Y.J. Oh, C.G. Park, C.Y. Hwang, and S.S. Kim. 2009. Pheromone trap type and height for attracting of Riptortus clavatus (Thunberg) (Hemiptera: Alydidae) in soybean field. Korean J. Appl. Entomol. 48(1):59-65. https://doi.org/10.5656/KSAE.2009.48.1.059
  29. Rodriguez, H. and R. Fraga. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv. 17:319-39. https://doi.org/10.1016/S0734-9750(99)00014-2
  30. Rural Development Administration (RDA). 2003. Manual for Agriculture Investigation. Suwon, Korea. pp.420-423.
  31. Ryu, C.M., M.A. Farag, C.H. Hu, M.S. Reddy, H.X. Wei, P.W. Pare, and J.W. Kloepper. 2003. Bacterial volatiles promote growth in Arabidopsis. Proc. Natl. Acad. Sci. USA. 100:4927-4932. https://doi.org/10.1073/pnas.0730845100
  32. Weller, D. M. 1988. Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annu. Rev. Phytopathol. 26:379-407. https://doi.org/10.1146/annurev.py.26.090188.002115
  33. Woo, S.M., J.U. Woo, and S.D. Kim. 2007. Purification and characterization of the siderophore from Bacillus licheniformis K11, a multi-functional plant growth promoting rhizobacterium. Korean J. Microbiol. Biotechnol. 35:128-134.