• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.253-264
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• 2009

The purpose of seed certification is to preserve the genetic purity and identity of seed varieties. This study is to provide information concerning seed certification procedures and certification standards of Kentucky bluegrass especially used in golf courses. We analyzed data from the seed certification standards of three states (Washington, Idaho and Oregon) in U.S.A. The certification processes both field inspection and laboratory requirement satisfying the minimum seed quality standards. The seed harvesting field must be propagated with the specified class of seeds and requires an adequate isolated distance from other crops. Moreover, the field should be clean and free from the objectionable weeds. The seed analysis tests include a germination rate, a percentage of pure seed, contents of other crop seed, weed seed, and inert matter. The certification standards of the certified seed and the sod quality seed showed general similarity in all three states. The certification standards of the sod quality seed should have less than 0.02% of maximum weed seed. The certified seed should have less than 0.3% of maximum weed seeds. Those certification standards of seed quality should guaranty the quality of turfgrass establishment of golf course.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.372-377
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• 2018

This study was investigated the effect of immature rhizome production according to harvest times-based treatment method for seed production of Gastrodia elata. The results revealed that when the tuber weight of G. elata harvested in spring (GEHS) was ${\geq}100g$, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was 90.9%-94.8%, 3.1%-5.4%, and 10.1%-15.3%, respectively. When G. elata harvested in fall (GEHF) was treated at a low temperature for 4 weeks or more, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was 70.4%-87.6%, 2.2%-2.6%, 8.7%-9.5%, respectively. Therefore, to produce seeds and immature rhizomes, GEHS must have tubers of more than 100 g, whereas GEHF requires breaking dormancy by low-temperature treatment for 4 weeks or more. Compared with those of GEHS, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was lower in GEHF; however, it was higher than those in the natural germination state. Thus, it can be expected that G. elata can be produced throughout the year by ensuring that the seeds and immature rhizomes of G. elata are produced using a constant tuber weight and by breaking dormancy with low temperature treatment.

• Journal of Korean Society of Forest Science
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• v.109 no.4
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• pp.400-412
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• 2020

We analyzed the growth characteristics of naturally regenerated seedings of Larix kaempferi in order to propose a field-applicable regeneration method. Experimental sites were established at Gapyeong in Gyeonggi-do and at Bonghwa in Gyeongsangbuk-do. We analyzed seed inflow features, occurrence, and growth characteristics of natural seedlings for three years. The reserved seed-tree site producedthe greatest number of inflow seeds and followed by seed-tree site and clear-cut site. In addition, the reserved seed-tree method was found to be more efficient than other methods in evenly scattering seeds. The rate of seedling occurrence at treatment sites (e.g., harvesting and scratching) was 2.4%, which is 8.0 times higher than the seedling occurrence (0.3%) at the non-treatment sites. There were approximately 470,000 seedlings per hectare at the treatment sites in June of the first year after regeneration and approximately 78,000 seedlings per hectare in October of the third year. The average diameter of the root collar of the seedlings in the third year was 6.5 mm, and the average height of the seedlings was 50.4 cm. These results indicate that it is possible to create a secondary forest of L. kaempferi by natural regeneration if harvesting and scraping are implemented during seed fructification. Considering the rapid growth of L. kaempferi shown in this study, the proposed method would be an efficient reforestation technique.

• Tunnel and Underground Space
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• v.19 no.6
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• pp.526-533
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• 2009

This study presents Mine Green Framework(M.G.F) which can protect the slope of rock and tailings. M.G.F method provides the fiber frame which helps the growth of the plant in the barren site like mined rock slope. M.G.F system consists of the polyester fiber mat, soil, seeds and anchors for the attachment. The optimum rate of filling materials was figured out by many germination tests in order to improve the effect of filling materials and the optimum rate of filling materials was applied in four test sites individually. High rooting rate over 70% was confirmed in all field tests. Especially the moisturizer was the most important component of filling materials and it could make the better condition for the plants.

• Korean Journal of Environmental Biology
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• v.22 no.2
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• pp.265-272
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• 2004

Biological characteristics of anodic electrolyzed water were investigated in this study. Linear DNAs which were incubated at $4^\circ{C}$ and $25^\circ{C}$ for 10 mins in the anodic electrolyzed water were degraded about 40% and 50%, respectively. But the DNA was amplified pretty well without any degradation through polymerase chain reaction in the presence of anodic electrolyzed water. Protein degradation hardly occurred in the distilled water during entire incubation time of 7 days, while protein began to be degraded from 4 days in the anodic electrolyzed water. Rice seeds could germinate in the distilled water and anodic electrolyzed water with the same germination ratio, however, the anodic electrolyzed water inhibited the growth of roots and total length of rice seedlings in the soil. Anodic electrolyzed water did not affect the growth curve and cell number of marine alga significantly. The anodic electrolyzed water inhibited the browning of potato by inactivating 50% of polyphenol oxidase activity.

• Journal of Sericultural and Entomological Science
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• v.37 no.1
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• pp.1-5
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• 1995

The fertilities of indigenous and cultivated mulberry species(varieties) which were polli nated with Morus nigra L. brought from Turkey were investigated. The percentage of ripening sorosis was very high in indigenous species, Morus tiliaefolia Makino and cultivated variety, Keomseolppong and it was around 50% in both Daeryukppong and Cheongilppong However, the flowers of Morus bombycis Koidz. (BOM5-8) and Yongcheonppong fell after pollination. Looking at the sizes of seeds, they are also various. Generally, it was extremely small in Morus tiliaefolia, but it was medium in Keomseolppong, Cheongilppong and Daeryukppong. The fertilization percentage was generally low with 32-42% in the varieties, but it was comparatively high in Keomseolppong and Morus tiliaefolia. The seed germination percentages of Daeryukppong and Morus tiliaefolia are 88.0% and 23.6%, respectively, but it was below 2% in Cheongilppong and Keomseolppong. The cross success percentages are 14.2% and 8.0% in Daeryukppong and Morus tiliaefolia, respectively, but it was below 0.3% in Cheongilppong and Keomseolppong.

• The Korean Journal of Mycology
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• v.19 no.3
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• pp.231-237
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• 1991

This study was conducted in order to find out biological control of sesame wilt and blight caused by Fusarium of oxysporum f. sp. vasinfectum and Phytophthora nicotianae var. parasitica by using Streptomyces spp. Two sesame pathogens, Fusarium oxysporum f. sp. vasinfectum and Phytophthora nicotianae var. parasitica were purely isolated from diseased sesame plants of the field. Streptomyces species were isolated from 72 soil samples collected from red pepper and sesame uplands in Chungbuk and selected as antagonists according to the results of dual culture. The selected Streptomyces isolates such as St-11 and St-20 were confirmed their antagonistic effect through mycelial inhibition zone and inhibitory effects on the mycelial growth of the pathogens by culture filterate of the antagonists. Inhibitory effects on the conidial germination of Fusarium oxysporum vasinfectum and Phytophthora nicotianae parasitica by the antagonists were also tested in addition to mycelial Iysis. The antagonists St-11 and St-20 showed inhibitory effect on growth of sesame seedlings after seeds soaked in the suspension. Effect of soil inoculation with antagonist St-11 showed 40 to 78 percent of control effect for two diseases in comparison with control under greenhouse.

• Korean Journal of Environmental Biology
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• v.18 no.1
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• pp.47-51
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• 2000

Phytophthora blight of pepper, which is caused by Phytophthora capsici Leonian, is not only the most destructive disease worldwide, but also difficult to control effectively. It has been needed to have new trials for effective control to the disease. We employed radiation hormesis of gamma ray as the new trial in the control strategy. Two cultivars, Kwangbok and Dabok, were used to analyse whether gamma ray radiation can induce disease resistance. The germination rate of pepper seeds was significantly enhanced by the radiation at all dose levels. Stimulatory effect for resistance induction was found to differ between cultivars. It was confirmed that the remarkable effect was induced in Dabok and depended on radiation dosage. Disease resistance at 4 Gy was much higher than that of control. On the other hand, no detectable induction effect for resistance was observed in Kwangbok which was moderate resistant cultivar to gamma ray radiation. [Hormesis, Gamma ray, Pepper, Phytophthora blight, Resistance induction].

• Journal of Bio-Environment Control
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• v.6 no.3
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• pp.225-234
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• 1997

This study was carried out to investigate the native distribution of wax myrtle (Myrica rubra) in Cheju Province and its propagation method. Wax myrtle is heavily distributed at 100 to 400m above sea level of Donhong-chun and Hyodon-chun which is bordered by Youngchon-dong of Seogwipo City and Haryeri of Namcheju county. BA, IBA, Kinetin, IAA, and GA3 were applied to promote rooting of cutting but were ineffective in promoting rooting. Treating cuttings with both AgNO,1 which removes rooting inhibitor, tannin and growth regulators such as BA, IBA and Kinetin were also ineffective in promoting rooting. Incubating seeds at 4$0^{\circ}C$ for 30 days resulted in 39％ germination. There was distinct difference in leaf shape between seedlings and mature trees.

• Korean Journal of Food Science and Technology
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• v.20 no.5
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• pp.650-658
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• 1988

The mobilization of proteins in the cotyledons of germinating soybean seeds (Glycine mar [L.] Merr.) and seedlings was studied by using light microscopy and transmission electron microscopy. The cotyledon tissues of soybean. were packed with protein bodies(diameter $0.1-15{\mu}m$) where storage protein of soybean is deposited. Degradation of protein bodies started in the epidermis and vascular tissues. After swelling of the protein bodies, autolysis of storage proteins began while the external membrane remained unbroken. Hydrolysis of proteins could be internal or peripheral and fusion might begin before complete protein degradation. Possible instances of vacuolar fusion were encountered in some cells. In all cases, the result of degradation was the same; the central vacuole of the cell. At the late stages of seedling growth, breakdown of tonoplast was observed in some cells.