• Title/Summary/Keyword: Seedling method

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Development of Efficient Screening Methods for Resistant Cucumber Plants to Meloidogyne incognita (오이 뿌리혹선충병에 대한 효율적인 저항성 검정법 확립)

  • Hwang, Sung Min;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Jin-Cheol;Choi, Gyung Ja
    • Research in Plant Disease
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    • v.20 no.2
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    • pp.119-125
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    • 2014
  • Root-knot nematodes represent a significant problem in cucumber, causing reduction in yield and quality. To develop screening methods for resistance of cucumber to root-knot nematode Meloidogyne incognita, development of root-knot nematode of four cucumber cultivars ('Dragonsamchuk', 'Asiastrike', 'Nebakja' and 'Hanelbakdadaki') according to several conditions such as inoculum concentration, plant growth stage and transplanting period was investigated by the number of galls and egg masses produced in each seedling 45 days after inoculation. There was no difference in galls and egg masses according to the tested condition except for inoculum concentration. Reproduction of the nematode on all the tested cultivars according to inoculum concentration increased in a dose-dependent manner. On the basis of the result, the optimum conditions for root-knot development on the cultivars is to transplant period of 1 week, inoculum concentration of 5,000 eggs/plant and plant growth stage of 3-week-old in a greenhouse ($25{\pm}5^{\circ}C$). In addition, under optimum conditions, resistance of 45 commercial cucumber cultivars was evaluated. One rootstock cultivar, Union was moderately resistant to the root-knot nematode. However, no significant difference was in the resistance of the others cultivar. According to the result, we suggest an efficient screening method for new resistant cucumber to the root-knot nematode, M. incognita.

Propagation of Cutting Method of a Rare Endemic Wikstroemia ganpi (Sieb. et Zucc.) Maxim. in Korea (희귀수종 거문도닥나무(Wikstroemia ganpi (Sieb. et Zucc.) Maxim.)의 번식에 관한 연구)

  • Yoon, Jung Won;Yi, Myung Hoon;Sung, Jung Won
    • Korean Journal of Plant Resources
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    • v.33 no.4
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    • pp.303-310
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    • 2020
  • This study was conducted on propagation use for conservation scheme of a threatened plant; Wikstroemia ganpi (Sieb. et Zucc.) Maxim(Geomundo false ohelo). The seed propagation was showed higher (95%) in storage and lower values in ground and cutting. Softwood cutting was higher than hardwood cutting and it was possessing higher ratio of rooting that increase concentration of IBA and NAA. It was determined that for Geomundo false ohelo seedling was effective than cutting. In-situ and ex-situ conservation and restoration of substitute habitats of Geomundo false ohelo is therefore necessary due to human trampling in the habitats, damage, natural selection, loss and suppression.

Invasion of Korean Pine Seedlings Originated from Neighbour Plantations into the Natural Mature Deciduous Broad-leaved Forest in Gwangneung, Korea (광릉 천연활엽수 성숙림에서 주변 인공림으로부터 잣나무 치수의 침입 정착)

  • Kang, Ho Sang;Lim, Jong-Hwan;Chun, Jung Hwa;Lee, Im Kyun;Kim, Young Kul;Lee, Jae Ho
    • Journal of Korean Society of Forest Science
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    • v.96 no.1
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    • pp.107-114
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    • 2007
  • Establishments of the seedlings inside the natural forest from adjacent artificial forests would be an important factor in forest stand dynamics. This study was conducted to see the invasion of Korean pine (Pinus koraiensis) seedlings which is not native in this region, into the natural deciduous broad-leaved forest in Gwangneung, Korea. There is no mother tree at the I ha study site while the number of naturally regenerated P. koraiensis seedlings was 345 trees and 56% of them were clumped with more than two seedlings at each point. Applying the image segmentation method to IKONOS satellite image of January, 2003, the distance from the center of 1 ha study site to the nearest mother tree and plantation of Korean pine were 200 m and 270 m, respectively. The average height and root-collar diameter of the seedlings were 34 em and 7 mm, respectively and the age of 207 seedlings (60%) were below 5 years old. Most abundant range of soil moisture gradient and LAl (leaf area index) were from 16 to 20% and those of LAI were from 3.1 to 3.5. To understand the dynamics and seed dispersal pattern of Korean pine in the Gwangneung natural deciduous broad-leaved forests, additional studies not only long-term monitoring of growth and mortality of naturally regenerated Korean pine seedlings but also application of stable isotope analysis and molecular genetic techniques was recommended.

Effect of Seeding Methods to the Growing Alopecurus aequalis var. amurensis in Wheat Field of Rice-wheat Cropping System (이모작 논 밀 재배시 파종방법이 뚝새풀의 생장에 미치는 영향)

  • Kim, Sun;Ahn, Seung-Hyeon;Im, Il-Bin;Cheong, Young-Keun;Kim, Si-Ju
    • Korean Journal of Weed Science
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    • v.30 no.3
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    • pp.252-257
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    • 2010
  • The experiments were carried out to develop ecological weed control system in wheat field of rice-wheat cropping system. The results were, depression effect on water foxtail by wheat seeding method decreased the seeding after doing soil rotary by 85% compared with the no practices. The occurrence of Water foxtail was 40 piece $m^{-2}$ on October 16, 29 piece $m^{-2}$ on October 26, and 4 piece $m^{-2}$ when surveying based on the standard of seeding the wheat at 15kg $ha^{-1}$ which decreased as the seeding time got later. According to the wheat seeding quantity 29 piece $m^{-2}$ occurred at 150kg $ha^{-1}$, and 8-11 piece $m^{-2}$ decreasingly at more than 200 kg $ha^{-1}$ of wheat seedlings based on seeding on October 26. As a result of summarizing the above results, crushing the soil by use of rotary before seeding wheat against the end of October, and seeding by increasing the seedling quantity (200 kg $ha^{-1}$) it is judged that the competition damage by weeds including water foxtail can be reduced without any use of herbicide.

Weed Control System, Yield and Quality Characteristic by Cropping System in Wet-Hill-Seeding Use Black Colored Rice (흑미 무논점파 재배방법에 따른 수량 및 품질특성과 잡초방제체계)

  • Cho, Seung-Hyun;Lee, Deok-Ryeol;Lee, Song-I;Lee, Ki-Kwon
    • Weed & Turfgrass Science
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    • v.6 no.3
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    • pp.196-202
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    • 2017
  • This study was conducted to investigate the change of yield and quality according to seeding time and planting density and to determine effective weed control method in wet-hill-seeding use black colored rice. The most effective weed control system was application of bromobutide thiobencarb five days before seeding followed by bensulfuron mefenacet thiobencarb 12 days after seeding (barnyardgrass at 2.5-3.0 leaf stage). The seedling stand by seeding time and planting density increased with the delay seeding time and high planting density. The heading dates were delayed as the seeding time became late but no difference was found between planting density. The anthocyanin content of black colored rice was higher at late seeding time and lower planting density. The yield of full colored rice was high 80 plants per $3.3m^2$ in seeding on May 30 and June 10. This information could be useful for spreading rice direct seeding by inducing stabilization of wet-hill-seeding use black colored rice.

Research Status for Drought Tolerance in Maize (옥수수 한발 내성에 관한 연구 현황)

  • Kim, Kyung-Hee;Moon, Jun-Cheol;Kim, Jae-Yoon;Kim, Hyo-Chul;Shin, Seung-Ho;Song, Ki-Tae;Lee, Byung-Moo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.4
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    • pp.401-411
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    • 2015
  • Drought stress has detrimental effects on the seedling development, vegetative/ reproductive growth, photosynthesis, root proliferation, anthesis, anthesis-silking interval (ASI), pollination and grain yield in maize. Typically, two weeks before silking through pollination are an important time in maize life. Here we reviewed the effects of drought stress on growth, physiological/ molecular researches for drought tolerance, and breeding to genomics in maize. Drought stress during kernel development increases leaf dying and lodging, decreases grain filling period and grain yield. Physiological factors of drought stress/ effects are water content, water deficits, and water potential. Nowdays molecular marker assisted breeding method is becoming increasingly useful in the improvement of new germplasm with drought stress tolerance.

Growth and Yield Variation of Clay-coated Rice Seeds in Direct Seeding Culture on Dry Paddy

  • Choi, Weon-Young;Park, Hong-Kyu;Ku, Bon-IL;Mo, Young-Jun;Choi, Min-Gyu;Kim, Sang-Su;Kim, Chung-Kon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.3
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    • pp.292-296
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    • 2008
  • Clay-coated rice seeds (clay-coated seeds A and B) were directly sown on dry paddy and their growth and yield were compared with the normal drill-sown seeds on dry flat paddy. In clay-coated seeds, germination was 1 day earlier and the emergence rate was higher up to 5% than that of normal drill-sown seeds. But the apparent number of seedling stand per $m^2$ was lower than that of normal drill-sown seeds, which is due to the smaller amount of seeding in clay-coated seeds. At the early growth stage, the plant height of clay-coated seeds A was taller than that of drill-sown seeds, while the plant height of clay-coated seeds B was 0.7 cm shorter than that of drill-sown seeds. At the late growth stage, however, the difference was insignificant in both cases. The maximum tillering stage was 10 days earlier in drill-sown seeds. Lodging index was the lowest in clay-coated seeds B and there was no difference between clay-coated seeds A and drill-sown seeds. The ratio of stem base weight, culm diameter and culm wall thickness were higher in clay-coated seeds, while the lower internodes (4th, 5th and 6th) length was shorter in claycoated seeds than in drill-sown seeds. In clay-coated seeds, the number of panicle per $m^2$ was smaller, while the number of spikelet per panicle was a little larger than in drill-sown seeds. The rate of ripened grain and brown rice 1,000 grain weight were lower in the clay-coated seeds, thus the yield was $98{\sim}99%$ level of drill-sown seeds. Considering that the amount of seeding in clay-coated seeds was two-thirds of that in drill-sown seeds, it is expected that clay coating method could become an additional technique for direct seeding cultivation.

Effects of Salt and Drought Stresses on Seed Germination and Gene Expression Pattern in Tall Fescue (염과 건조 스트레스 조건에서 톨 페스큐의 종자 발아율과 유전자 발현 변화분석)

  • Lee, Sang-Hoon;Lee, Ki-Won;Choi, Gi Jun;Kim, Ki-Yong;Ji, Hee Jung;Hwang, Tae Young;Lee, Dong-Gi
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.34 no.2
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    • pp.114-119
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    • 2014
  • Salinity and drought stresses are probably the most significant abiotic factor limiting plant's growth, also negatively affect seed germination and early seedling development. To study on effect of NaCl and PEG stress on seed germination and gene expression pattern of tall fescue, the levels of NaCl and PEG-induced water stresses were determined in first experiment. Different concentration of NaCl (0 to 350 mM) and PEG (0 to 30%) were used for seed treatment. Seed Germination percentage reduced with increasing osmotic potential of growth medium either due to NaCl or PEG. Seeds were not germinate at 350 mM NaCl or 30% PEG treatment. On the basis of the results, Kentucky31(E-) had more resistant than Fawn in both stress conditions. Furthermore, we have used an annealing control primer-based differential display reverse transcription-polymerase chain reaction method to identify salt- and drought stress-induced differentially expressed genes (DEGs) in tall fescue leaves. Using 120 annealing control primers, a total of 4 genes were identified and sequenced. The possible roles of the identified DEGs are discussed in the context of their putative role during salinity and drought stresses.

The Growth of the Cultured Scallop, Patinopecten yessoensis (양식 가리비의 성장)

  • Yoo Sung Kyoo;Ryu Ho-Young;PARK Kyung-Yang
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.14 no.4
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    • pp.221-226
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    • 1981
  • The seedlings of the scallop, Patinopecten yessoensis, which were collected in April 1980, April 1981 and May 1981, and the grown-up scallops collected in the sea bottom in November 1979, were reared by hanging method up to the July 1981. The growth of the seedlings collected in April 1980 was greatly affected by the time of the seedling collection, manipulation of the net cage, density in the net cage and the annual water temperature fluctuation. The scallops reared from the spat collected in April 1980 reached the sizes of 0.33 mm, 1.23 mm, 29.34 mm and 59.59 mm in shell length in 40,75, 285 and 450 days respectively. Since then, growth rate was determined as follows based on the age estimated by the year rings on the shell:84.96mm in 19 months, 99.3mm in 31, 112.3mm in 37 and 113.64mm in 43 months. The meat and the adductor muscle weight increased with the shell length. The meat weight roached about 15g when the shell length was 60-70mm and about 94.13 g when 130-140mm, and the adductor muscle weight reached about 4.89 g when the shell length was 60-70 mm and about 39.59g when 120-130 mm. But the growths of the meat and the adductor muscle weight were in stagnancy after scallops reached 125 mm in shell length.

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Methodological Consideration for Estimating Growing Stock of Young Forests based on Early Growth Characteristics of Standing Trees in Korea (우리나라 입목의 초기 생장 특성에 따른 유령림의 임목축적 산출방안 고찰)

  • Moon, Ga Hyun;Moon, Na Hyun;Yim, Jong Su;Kang, Jin Taek
    • Journal of Korean Society of Forest Science
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    • v.109 no.3
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    • pp.300-312
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    • 2020
  • The growing stocks of young forests that are less than10 years of age have been excluded from the Korean forest resource statistics, despite the existence of standing trees; however, sustainable forest management and carbon removals in the forestry section require complete information regarding forest resources. This study developed a method to estimate the growing stocks for young forests from National Forest Inventory (NFI) data. After reviewing previous research on growth characteristics for young forests, we conducted stem analysis of major species, and examined stand characteristics by site index, based on real yield tables. Our statistical analysis results showed that there were few standing trees with diameters at breast height (DBH) above 6 cm in young stands, and that it would have taken 12 years, on average, to reach 6 cm DBH. This suggests that mean tree height by diameter should be assessed at the root, in order to assess growing stocks for young stands through the NFI. Moreover, the database system should be improved to differentiate tree species, since diverse shrubs, including trees, have been surveyed.