Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of Efficient Screening Methods for Resistant Cucumber Plants to Meloidogyne incognita

오이 뿌리혹선충병에 대한 효율적인 저항성 검정법 확립

  • Hwang, Sung Min (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Jang, Kyoung Soo (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Choi, Yong Ho (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Cheol (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Choi, Gyung Ja (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
  • 황성민 (한국화학연구원 바이오화학연구센터) ;
  • 장경수 (한국화학연구원 바이오화학연구센터) ;
  • 최용호 (한국화학연구원 바이오화학연구센터) ;
  • 김진철 (한국화학연구원 바이오화학연구센터) ;
  • 최경자 (한국화학연구원 바이오화학연구센터)
  • Received : 2014.02.04
  • Accepted : 2014.05.14
  • Published : 2014.06.30
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Abstract

Root-knot nematodes represent a significant problem in cucumber, causing reduction in yield and quality. To develop screening methods for resistance of cucumber to root-knot nematode Meloidogyne incognita, development of root-knot nematode of four cucumber cultivars ('Dragonsamchuk', 'Asiastrike', 'Nebakja' and 'Hanelbakdadaki') according to several conditions such as inoculum concentration, plant growth stage and transplanting period was investigated by the number of galls and egg masses produced in each seedling 45 days after inoculation. There was no difference in galls and egg masses according to the tested condition except for inoculum concentration. Reproduction of the nematode on all the tested cultivars according to inoculum concentration increased in a dose-dependent manner. On the basis of the result, the optimum conditions for root-knot development on the cultivars is to transplant period of 1 week, inoculum concentration of 5,000 eggs/plant and plant growth stage of 3-week-old in a greenhouse ($25{\pm}5^{\circ}C$). In addition, under optimum conditions, resistance of 45 commercial cucumber cultivars was evaluated. One rootstock cultivar, Union was moderately resistant to the root-knot nematode. However, no significant difference was in the resistance of the others cultivar. According to the result, we suggest an efficient screening method for new resistant cucumber to the root-knot nematode, M. incognita.

연작 재배로 인하여 뿌리혹선충(Meloidogyne incognita)과 같은 식물 기생 선충에 의한 피해는 오이 재배 면적이 늘어나면서 크게 늘어나고 있다. 이러한 생산량의 감소 피해를 해결하기 위한 친환경적인 방제 방법으로 경종적 방제의 저항성 품종을 이용한 재배가 점차 증가되고 있다. 본 연구에서는 새로운 저항성 육종 소재를 찾기 위한 효율적인 저항성 검정 방법을 확립하기 위하여, 뿌리혹선충 M. incognita를 이용한 오이의 선충병 발생 정도를 접종원 농도, 오이 생육 시기 및 이식 시기 등의 다양한 발병 조건에 따라 오이 4개 품종('드레곤삼척', 아시아스트라이크', '네박자', '하늘백다다기')을 대상으로 조사하였다. 접종원의 접종 농도가 증가 할수록 오이의 뿌리혹선충병 발생은 농도 의존적으로 증가되었지만, 오이의 생육 시기 및 이식 시기에 따른 오이의 뿌리혹선충병 발생은 유의한 차이가 없었다. 따라서, 오이 품종의 뿌리혹선충병 저항성 검정 조건으로 온실($25{\pm}5^{\circ}C$)에서 파종 후 21일간 재배하고, 이식한지 7일 후에 5,000개의 뿌리혹선충 알 접종으로 45일 이후에 병 조사를 하는 것이 적합함을 알 수 있었다. 적정한 발병 조건에 따라 시판 중인 오이 45개 품종의 뿌리혹선충에 대한 저항성 정도를 조사한 결과, 대목 6개 품종에서 난낭수 100개 이하의 뿌리혹선충병 발생 정도를 보였고, 특히 '유니온' 대목 품종은 중간 저항성으로 조사되었다. 그 외 실험한 모든 품종들은 유사한 정도의 높은 감수성을 나타냈다. 이들 결과들을 바탕으로 M. incognita에 대한 오이 품종의 저항성 정도를 검정하기 위한 효율적인 방법을 제안하고자 한다.

Keywords

References

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