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http://dx.doi.org/10.5333/KGFS.2014.34.2.114

Effects of Salt and Drought Stresses on Seed Germination and Gene Expression Pattern in Tall Fescue  

Lee, Sang-Hoon (National Institute of Animal Science, Rural Development Administration)
Lee, Ki-Won (National Institute of Animal Science, Rural Development Administration)
Choi, Gi Jun (National Institute of Animal Science, Rural Development Administration)
Kim, Ki-Yong (National Institute of Animal Science, Rural Development Administration)
Ji, Hee Jung (National Institute of Animal Science, Rural Development Administration)
Hwang, Tae Young (National Institute of Animal Science, Rural Development Administration)
Lee, Dong-Gi (Division of Life Sciences, Korea Basic Science Institute)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.34, no.2, 2014 , pp. 114-119 More about this Journal
Abstract
Salinity and drought stresses are probably the most significant abiotic factor limiting plant's growth, also negatively affect seed germination and early seedling development. To study on effect of NaCl and PEG stress on seed germination and gene expression pattern of tall fescue, the levels of NaCl and PEG-induced water stresses were determined in first experiment. Different concentration of NaCl (0 to 350 mM) and PEG (0 to 30%) were used for seed treatment. Seed Germination percentage reduced with increasing osmotic potential of growth medium either due to NaCl or PEG. Seeds were not germinate at 350 mM NaCl or 30% PEG treatment. On the basis of the results, Kentucky31(E-) had more resistant than Fawn in both stress conditions. Furthermore, we have used an annealing control primer-based differential display reverse transcription-polymerase chain reaction method to identify salt- and drought stress-induced differentially expressed genes (DEGs) in tall fescue leaves. Using 120 annealing control primers, a total of 4 genes were identified and sequenced. The possible roles of the identified DEGs are discussed in the context of their putative role during salinity and drought stresses.
Keywords
Tall fescue; Water stress; Seed germination; Gene expression; Grassland;
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