• Title/Summary/Keyword: Seedling elongation

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Effect of Crude Extracts from Allium spp. on Growth of Several Crop Seedling (파속 식물의 조추출물이 몇가지 작물의 유묘생육에 미치는 영향)

  • 최상태;안형근;장영득
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.5
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    • pp.526-534
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    • 1996
  • This study was conducted to find out the effects of crude extracts from welsh onion, onion, chinese chives and garlic on the seedling growth of chrysanthemum, lettuce, rice, radish, chinese cabbage, cucumber and oriental melon. The crude extracts from Allium spp. inhibited the growth of chrysanthemum and lettuceseedlings at low concentration, the effect increased as the concentration grew higher, and especially in lettuce, the radicle growth was inhibited greater than the hypocotyl growth.The root extracts from welsh onion, chinese chives, and stem-leaf extracts from welsh onion, chinese chives and garlic significantly promoted the root growth of rice seedlings at 300∼700ppm and at 500∼1,000ppm, respectively, and the leaf sheath was elongated at low concentration of all extracts. The stem-leaf extracts from garlic and onion promoted the root and hypocotyl growth of chinese cabbage up to 2,000ppm or 3,000ppm. Root extracts from all Allium spp. promoted the elongation of chinese cabbage root up to 300∼700ppm, but they didn't influence its hypocotyl elongation. At low concentration of onion, garlic and chinese chives extracts, the growth of radish seedlings was highly promoted. The promotive effect, however, declined as the concentration increased. Only, extracts of chinese chives promoted the growth of radish seedling up to 5,000ppm. The stem-leaf extracts from onion and garlic promoted hypocotyl growth in pepper, up to 5,000ppm, but had little effect on other treatments. All of the extracts markedly promoted both root(main and lateral root) and hypocotyl growth in cucumber seedlings. A higher degree of promotion was made in the cucumber lateral root by onion and garlic extracts. The seedling growth of oriental melon was slightly increased by low concentration, but high concentration inhibited the root and hypocotyl growth.

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Allelopathic Effects of Fir Tree (Abies holophylla)

  • Kim, Young-Sik;Kil, Bong-Seop
    • The Korean Journal of Ecology
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    • v.27 no.5
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    • pp.307-310
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    • 2004
  • It was found that seed germination and seedling growth of selected species were inhibited by phytotoxic substance released from fir trees. The aqueous extracts of leaves caused significant inhibition in the seed germination of the receptor plants, Whereas seed germination of some species was not inhibited in extracts of leaves, but seedling elongation of the receptor plants was also decreased by the aqueous extract. Dry weight growth was slightly increased in lower concentration of the extract, while that was proportionally inhibited by higher concentration of the extracts. Chemical substances of fir trees were shown the biological toxicity. The GC method was employed for analysis of phytotoxic chemicals and sixteen chemical substances were identified such as benzoic, phenylacetic, etc. Also 33 volatile substances were identified from the fir tree. These chemical compounds are assumed the substances related to allelopathic potential of Abies holophylla plant species.

A Bioassay on Susceptivity of Selected Species to Phytotoxic Substances from Tomato Plants (토마토식물의 독성물질에 대한 타 식물의 민감도 검정)

  • 김영식
    • Journal of Plant Biology
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    • v.30 no.1
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    • pp.59-67
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    • 1987
  • To verify allelopathic effect of tomato plants a number of labovatory experiments, seed germination and seedling growth with aqueous extracts and leachates form the tomato plants, have performed. Germination percentage of the tested species was decreased by treatment with leaf, stem and root extracts as extracting time elapses. The leaf extract more inhibits the germination of the seeds than both stem and root extracts. Leachate collected from the tomato plants decreases as much as 50% of the growths in elongation as well as dry weight of seedlings of lettuce and egg plant. It is observed that with a paired-plants grown in U tube pot the tomato roots have excreted allelochemicals to inhibit the growth of the tested species and volatile substances from the tomato plants have suppressed to dry weights of lettuce, to elongation and dry weights of grapevine planted near the tomato plants.

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High Frequency Regeneration of Plantlets from Seedling Explants of Asteracantha longifolia (L.) NEES

  • Mishra Ramya Ranjan;Behera Motilal;Kumar Deep Ratan;Panigrahi Jogeswar
    • Journal of Plant Biotechnology
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    • v.8 no.1
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    • pp.27-35
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    • 2006
  • Plantlet regeneration in Asteracantha longifolia(L.) Nees (Acanthaceae), a medicinal herb has been achieved from seedling explants on basal MS medium. Three different seedling explants including node, internode and leaf segments on used. Of these three explant, leaf explants gave better response for both callus mediated organogenesis and direct multiple shoot induction. Number of explants showing differentiation of shout buds was higher on MS media supplemented with BA compared to kinetin. MS medium fortified with BA ($2.0mgl^{-1}$) and NAA ($0.5mgl^{-1}$) was found to be most suitable for both callus mediated organogenesis and elongation of shouts. The elongated shoots were successfully routed on MS medium fortified with NAA or IBA. Among them $0.1mgl^{-1}$ NAA or $0.2mgl^{-1}$ IBA provides better response for rhizogenesis. Regenerated plantlets were successfully established in soil where 85.4% or them developed into morphologically normal and fertile plants. RAPD profiling using four decamer primers confirmed the genetic uniformity of the regenerated plantlets and substantiated the efficacy and suitability of this protocol for in vitro propagation of A. longifolia.

β-Amino-n-butyric Acid Regulates Seedling Growth and Disease Resistance of Kimchi Cabbage

  • Kim, Yeong Chae;Kim, Yeon Hwa;Lee, Young Hee;Lee, Sang Woo;Chae, Yun-Soek;Kang, Hyun-Kyung;Yun, Byung-Wook;Hong, Jeum Kyu
    • The Plant Pathology Journal
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    • v.29 no.3
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    • pp.305-316
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    • 2013
  • Non-protein amino acid, ${\beta}$-amino-n-butyric acid (BABA), has been involved in diverse physiological processes including seedling growth, stress tolerance and disease resistance of many plant species. In the current study, treatment of kimchi cabbage seedlings with BABA significantly reduced primary root elongation and cotyledon development in a dose-dependent manner, which adverse effects were similar to the plant response to exogenous abscisic acid (ABA) application. BABA was synergistically contributing ABA-induced growth arrest during the early seedling development. Kimchi cabbage leaves were highly damaged and seedling growth was delayed by foliar spraying with high concentrations of BABA (10 to 20 mM). BABA played roles differentially in in vitro fungal conidial germination, mycelial growth and conidation of necrotroph Alternaria brassicicola causing black spot disease and hemibiotroph Colletotrichum higginsianum causing anthracnose. Pretreatment with BABA conferred induced resistance of the kimchi cabbage against challenges by the two different classes of fungal pathogens in a dose-dependent manner. These results suggest that BABA is involved in plant development, fungal development as well as induced fungal disease resistance of kimchi cabbage plant.

The Brassica rapa Rubber Elongation Factor Promoter Regulates Gene Expression During Seedling Growth in Arabidopsis thaliana and Brassica napus

  • Hong, Joon Ki;Lim, Myung-Ho;Kim, Jin A;Kim, Jung Sun;Lee, Seung Bum;Suh, Eun Jung;Lee, Soo In;Lee, Yeon-Hee
    • Plant Breeding and Biotechnology
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    • v.2 no.3
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    • pp.289-300
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    • 2014
  • A tissue-specific and developmentally expressed gene was isolated from Chinese cabbage (Brassica rapa L. ssp. pekinensis), designated BrREF (B. rapa Rubber elongation factor). BrREF transcripts were expressed at high levels in seedlings and at low levels in flower buds and roots. To study the activity of this promoter, the 2.2 kb upstream sequence of BrREF gene was fused to a β-glucuronidase (GUS) reporter gene and was introduced into Arabidopsis thaliana and B. napus by Agrobacterium-mediated transformation. Strong expression of GUS driven by the BrREF promoter was detected in the cotyledons and hypocotyls of transgenic plant seedlings, but GUS expression was weak in roots, excluding the root tips. GUS expression in the cotyledons and hypocotyls decreased dramatically as the seedlings matured and was not detected in the tissues of mature plants. During floral development, GUS expression was observed in immature anthers. These findings suggest that the BrREF promoter can modulate the tissue-specific and developmental expression of gene at the early stages of growth and development.

Influences of DIF on Growth and Development of Plug Seedlings of Lycopersicon esculentum before and after Transplanting (주야간 온도차(DIF)에 따른 토마토 플러그묘의 생장과 정직후 생육)

  • 임기병;손기철;정재동
    • Journal of Bio-Environment Control
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    • v.6 no.1
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    • pp.34-42
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    • 1997
  • Effects of DIF on stem elongation in plug seedlings of mini tomato ‘Mini Carol’ and on the growth and flowering after transplanting were investigated. Stem elongation of seedlings was mainly influenced by day temperature rather than night temperature. When the effect of +DIF was compared to that of -DIF under the same average daily temperature(ADT), day temperature had greater impact on internode elongation than night temperature. On the other hand, leaf unfolding rate increased and compactness decreased as ADT in creased. Differences in internode length affected by DIF during seedlings stage DIF progressively diminished during growth period after transplanting. Node position at which first flower was initiated was lowered as ADT decreased. It was concluded that DIF treatment was an applicable tool for control of stem elongation, particularly to reduce stem length without a noticeably adverse effect on the growth and development of plant after transplanting.

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Studies on scattering of Rice before Barley Harvest 4. Variation of dissolved chemical components in extracted solution under barley straw mulching and their effects on varietal germination rate and seedling elongation (보리 입모중 벼 파종에 관한 연구 4. 보리짚 피복량에 따른 담수중의 함유성분 변이와 발아율 및 유묘 신장의 품종간 차이)

  • Cheong J. I.;Kim B. K.;Lee J. K.;Shin H. T.
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1995.10a
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    • pp.70-71
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    • 1995
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Inhibitory Effect of Alantolactone on the Growth of Plant and Interaction with L-Cysteine (Alantolactone이 식물생장에 미치는 영향과 L-Cysteine과의 상호작용)

  • 권영명
    • Journal of Plant Biology
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    • v.17 no.1
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    • pp.9-14
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    • 1974
  • Inhibitory effect of alantolactone and isoalantolactone was shwon in Avena straight growth test and in the formation of adventitious root in Phaseolus seedling. However, di-, and tetrahydroalantolactones given no effect on the elongation and the rooting. Inhibitory effect of alantolactone could partly be removed by cysteine, cystine, and reduced glutthione. The plant materials were made less sensitive to alantolactone by the pretreatment of cysteine, but cysteine supplied after the treatment of alantolactone brought about no effect on the action of alantolactone. A new spot was shown on TLC plate from the mixture of alantolactone and cysteine, indicating that alantolactone can be inactivated by cysteine, not cystine, without any biological processes.

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