• The Korean Journal of Mycology
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• v.31 no.1
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• pp.44-49
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• 2003

Several microbial biocontrol products (Greenbiotech Co., Paju, Korea), Green-all T (Trichoderma harzianum), Green-all S(Bacillus sp.) and Green-all G (Streptomyces sp.) were supplemented with organic amendments such as sawdusts and rice hulls to study on efficacy of biological control of seeding damping-off of cucumber caused by Pythium ultimum. Sawdusts amended into potato dextrose agar alone could inhibit in vitro mycelial growth of P. ultimum. All there microbial products of Green-all T, Green-all G and Green-all S significantly reduced seeding damping-off (LSD, P=0.05). However, several amendments such as sawdusts and rice hulls into Green-all T and Green-all S products did not increase efficacy of biological control compared to non-amended treatment. In contrast, supplements of aminodoctor containing several amino acids (Greenbiotech Co., Korea) into Green-all G product significantly increased efficacy of biological control of seeding damping-off, resulting in from 42% to 2% disease incidence in relation to seedling emergence (LSD, P=0.05). Also, amendment of sawdusts into Tricoderma product significantly increased efficacy of biological control as disease index of 5.0 compared to non-amended control of 56.0 in Green-all T product alone. This indicates that organic amendments could increase efficacy of biological control of cucumber seedling damping-off.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.2
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• pp.117-121
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• 2001

This study was carried out to investigate the effects of rice cultivation of no-tillage seeding on the physicochemical properties and rice yield in the paddy soil of Milk vetch (Astragalus sinicus L.) established. The number of Milk vetch establishment was 256 per $m^2$ in the before experiment but 440 per $m^2$ in the after experiment. The seed numbers of Milk vetch were sufficient that Milk vetch seed were not sown again in the next year. Soil physicochemical properties of the plot of Milk vetch cultivation were similar to the conventional plot. The amount of weed occurrence was 87g dry weight per $m^2$ in the conventional but not occurrence in the sowing of Milk vetch establishment. Rice yield was 4.9ton per ha in the conventional and 4.5 ton per ha in the soybean seeder sowing of Milk vetch establishment.

• Korean Journal of Weed Science
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• v.8 no.1
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• pp.28-32
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• 1988

The effects of standing water and cultivation frequency on emergence of Echinochloa glabrescens Munro ex. Hook were determined at varied seeding methods under simulated field conditions for 4 months. In soil-incorporated seeding method total emergence for 4 months was highest in saturated condition followed by 4, 2, and 8cm standing water, whereas total emergence of seeds sown at the soil surface was highest at 2cm standing water followed by 4cm, saturated soil, and 8cm standing water. Within 2 weeks the emergence was highest at 4cm and lowest at 2cm standing water with the soil-incorporated seeding, but the seeds sown at the soil surface with 2cm standing water resulted in the highest emergence. Ratio of emergence within 2 weeks over total emergence during 4 months was lowest at 2cm standing water in the soil incorporation, indication that 2cm standing water would be the critical water level for E. glabrescens. Since most of the seedling was emerged within 2 weeks just before cultivation, the emergence pattern was little affected by cultivation at each standing water level.

• Weed & Turfgrass Science
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• v.1 no.4
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• pp.24-30
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• 2012

This study was conducted to select the cover crops in organic apple orchard by estimating the coverage of each cover crops and time-periodic weed occurrence. Seeding of Festuca myuros and Trifolium repens showed high contents of soil organic matter and phosphorus as compared with control. Seeding of Trifolium sp., Hordeum vulgare and Secale cereal reduced relatively occurrence of weed in May and S. cereal among others showed the greatest effect in June. In July, seeding F. arundinaceae and Poa pratensis showed low coverage and a lot of weeds occurred in their field. Fifty one species were occurred as weeds and it needs to removal of high and broadleaf weeds such as Chenopodium ficifolium, C. album var. centrorubru and Humulus japonicus in spring to increase the rate of ground cover. P. pratensis having the lowest cover rate showed the similar dry weight of weeds as compared with control. Trifolium sp. and Persicaria hydropiper had a high correlation with contents of soil organic matter and C. album var. centrorubru had a correlation with contents of soil phosphorus by CCA analysis.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.6
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• pp.334-338
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• 2006

This study was conducted to evaluate the effect of improvement of soil physical properties such as deep plowing, subsoil breaking and diagnostic fertilization on the yield of rice and nitrogen-use efficiency in direct seeding on flooded paddy surface of rice. The effects of deep plowing, subsoil breaking and diagnostic application of N, P, K fertilizers, Latex coated urea(LCU), compost, silicate were investigated. The soil physical properties, such as bulk density, hardness and porosity were improved and the content of organic matter and available $SiO_2$ were also increased by deep plowing and subsoil breaking. The amount of $NH_4-N$ in soil was highly increased by diagnostic fertilization and deep plowing at 5th leaf stage. The nitrogen-use efficiency was the highest at the diagnostic application of LCU 70% applied as basal dressing with subsoil breaking. The yield of rice increased by 8% under the diagnostic application of LCU 70% applied as basal dressing with subsoil breaking compared with the conventional application.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.5
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• pp.110-120
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• 2009

The hipped roof on the research building, which was constructed 51.9m above sea level on a hillock by Korea National University of Education in June 1999, is composed of four inclined planes which are 12m in breadth, 8m in length and have a 30 degree gradient. For the roof vegetation, the yellow earth collected from around the building was laid on top. It was designed to supply the soil on the slope with water for a considerable period by making rainfall pool at the edges. In order to prevent the soil on the slope from being swept away, 31 sorts of grass seeds were imported from Germany and sown in the soil. At the present day, 10 years after the seeds began to sprout and inhabit the settled slope, 30 individual plant species were identified in the period between April 2008 and March 2009. Out of 31 species were seeded on the slanted, soiled roof, only 8 were still alive. It was confirmed that the Artemisia Princeps var, Chrysantheum, Prunella Vulgaris and Lespedeza Cuneata have been the major species inhabiting the east, west, south and north inclined planes respectively. The Phragmites Communis was inhabiting the edge of the roof where the water supply was adequate, while the Dianthus Barbatus was primarily inhabiting the south-east side of the roof. As a whole, 26 identifiable plants and 4 unidentified plants were observed on the inclined planes of the hipped roof. In consideration of the plant distribution on the slope, it was confirmed that the selection of seeds may have had an effect on the slope vegetation. As for the yellow earth laid on the roof, it was discovered that about 2~3cm thickness around the ridge was swept away, but the rest of the slope was in relatively good condition. Accordingly, it has been proven that vegetations can be applied to hipped roofs by using ordinary plants without any special structural measures.

• Journal of Periodontal and Implant Science
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• v.41 no.2
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• pp.67-72
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• 2011

Purpose: The aim of this study is to determine whether certain biomaterials have the potential to support cell attachment. After seeding bone marrow stromal cells onto the biomaterials, we investigated their responses to each material in vitro. Methods: Rat bone marrow derived stromal cells were used. The biomaterials were deproteinized bovine bone mineral (DBBM), DBBM coated with fibronectin (FN), synthetic hydroxyapatite (HA), HA coated with FN, HA coated with $\beta$-tricalcium phosphate (TCP), and pure $\beta$-TCP. With confocal laser scanning microscopy, actin filaments and vinculin were observed after 6, 12, and 24 hours of cell seeding. The morphological features of cells on each biomaterial were observed using scanning electron microscopy at day 1 and 7. Results: The cells on HA/FN and HA spread widely and showed better defined actin cytoskeletons than those on the other biomaterials. At the initial phase, FN seemed to have a favorable effect on cell adhesion. In DBBM, very few cells adhered to the surface. Conclusions: Within the limitations of this study, we can conclude that in contrast with DBBM not supporting cell attachment, HA provided a more favorable environment with respect to cell attachment.

• Journal of Nutrition and Health
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• v.33 no.6
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• pp.660-667
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• 2000

Previous studies on the effect of age on intestinal taurine transport in animals have invariably shown a decline in the activity of the transport system with increasing age. In the present study changes in taurine transporter activity were observed during cell differentiation in the human colon carcinoma cell line HT-29 This cell line exhibits various enterocytic characteristics when differentiated and therefore has frequently been used to study the characteristcs and regulation of nutrient and drug absorption in the small intestine at the cellular level. Pre-treatment of the cells with $\beta$-alanine(10mM) reduced the taurine transport activity to 33% of the value for the control cells(p<0.05) which implies that taurine and $\beta$-alanine share a common $\beta$-amino acid transport system for their celluar uptake in the HI-29 was continued until 21 days post seeding. Kinetic studies of the taurine transporter were conducted in the HT-29 cell line with varying taurine concentration(5-60$\mu$M) in the uptake medium Both Vmax and the Michaelis-Menten constant(Km) of taurine transporter were decreased as differentiation of the HT-29 cell line was progressed ; Vmax of the taurine transporter in cells incubated for 4, 14 and 21 days post seeding was 2.79$\pm$3.4m 16.89$\pm$1.74, and 0.85$\pm$0.08 and 0.32$\pm$0.01nmol.mg protein-1 .30min-1 respectively(p<0.001) and Km was 42.3$\pm$3.4, 16.89$\pm$1.74, and 11.2$\pm$3.0$\mu$M respectively (p<0.01) These results indicate that the activity of sodium dependent active taurine transport system in the HT-29 cell line is decreased as confluent cells are differentiated. This phenomenon in cell culture system corresponds well with the earlier observation of lower intestinal taurine transport activity in suckling rats compared to that in adult animals although direct relationship of cell differentiation with in vivo aging process needs further verification.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.348-348
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• 2017

This experiment was conducted to investigate the aspect of tiller occurrence, growth and yield of sorghum according to planting density and sowing date. The subject of this experiment is to supply basic data to inhibit non-productive tillers uneconomical and cumbersome for mechanical harvesting. Also another subject was to evaluate optimum planting density and sowing date in central district area. Total number of tillers was more in 80cm ridge than 60cm ridge and it was increased as the planting distance was wider from 15cm to 30cm on the each ridge. Ratio of effective tillers was higher in 60cm ridge than 80cm ridge and it was decreased as planting distance was wider from 15cm to 30cm. The lower the planting density, the more increased total number of tillers, whereas effective tillers were decreased as planting density was high. Average of total number of tillers of three varieties was higher in sowing date of 2 May (1st sowing date), whereas ratio of effective tillers was the highest in sowing date of 23 May (2nd sowing date). Hwanggeumchal showed the highest total number of tillers (1.2 tillers), while Moktaksusu had the lowest total number of tillers (0.8 tillers) among three varieties. There were no significant difference between planting density and days to heading and ripening date from seeding. Culm length increased as planting density was high, but ear length, grains per ear and 1000 grain weight were decreased on the other hand. The highest yield of sorghum per 10a was obtained from $60{\times}20cm$ planting density among 6 planting densities.

• Korean Journal of Environmental Agriculture
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• v.17 no.2
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• pp.156-159
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• 1998

Germination rate and early growth in crop such as rice,soybean,and perilla were observed after irradiation of $^{\gamma}$-ray (Co-60) in order to determine the effects of low does radiation. The low dose radiation was able to improve the early growth in crops and their agricultural charaters. Germination rate of 2Gy-irradiatied rice seeds was high and also were seeding height and fresh weight of the 0.5 Gy-irradiated. Germination rate and early growth of soybean were high in 4Gy-irradiated group. Perilla gew ot so promisingly after after low dose irradiation,however there slightly increasing effects on germination rate, seeding height and fresh weightat 2Gt-, 1Gy-, and 1Gy irradiated group, respectively.