• Journal of Life Science
• /
• v.13 no.1
• /
• pp.15-20
• /
• 2003

Seeds treated before sowing should be dried for their quality control on their sale or storage. The experiments were done to clarify the effect of drying, temperature and light quality treated during drying and germination on germination of gourd (Lagenaria siceraria Standl.) seeds. Drying of treated seeds was done at $35^{\circ}C$. After treated seeds were dried. their germination test was done at 10, 20 and $30^{\circ}C$ on the condition of darkness, but with blue, red, far-red light and darkness as light quality treatments. FR-yongjadaemok and FR-kunghap were used as test cultivars and their daily germination rates were measured at treatment levels. Its presowing hydrated seeds were dried 10 hours at $35^{\circ}C$ until reached below their original moisture content, and germination rate between the above dried seeds and the conventional dry-heated ones was not significantly different. At $30^{\circ}C$ germination temperature, the treated seeds showed higher initial germination rate when dried under red light illumination than under darkness although their germination rates were nearly same at each temperature of 10 and $20^{\circ}C$ being relatively low. Effect of the red light treatment during their drying continued to more than 3 months. Germination rate of the red light treated seeds during drying was reduced by far-red light treated during their germination. The initial rate to the second day after sowing were higher under red light and darkness treated during germination than under the two other lights.

• Journal of Bio-Environment Control
• /
• v.21 no.1
• /
• pp.45-49
• /
• 2012

Chilean wild flower seeds showed low germination rate and lacked germination uniformity. This experiment was conducted to find out the effects of light conditions, $GA_3$ (50 mg/L, 100 mg/L, 200 mg/L) and $KNO_3$ (0.1%, 0.2%, 0.4%) treatments on germination of five species of Chilean wild flower seeds. The seeds of $Schizanthus$ $candidus$, $Schizanthus$ $hookerii$, and $Schizanthus$ $litoralis$ did not germinated in light condition. The others did not show a significant different germination rate under light and dark conditions. The germination rate of $Mimulus$ $luteus$, $Schizanthus$ $candidus$, $Schizanthus$ $hookerii$ increased 2.3, 5.6, and 3.2 times compared with control, respectively in $GA_3$-200 mg/L treatment, but that of $Rhodophiala$ $ananuca$ enhanced slightly in $GA_3$-50 mg/L treatment. Those results suggested that the germination rate of Chilean wild flower seeds enhanced by $GA_3$ treatment that is very efficient in breaking seed dormancy.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.49 no.6
• /
• pp.472-476
• /
• 2004

Lateral roots formed on mungbean sprouts should lower their quality. The study was carried out to clarify the effects of aeration periods (AP; 1, 2, 3, and 4 hours) and temperature (AT; 20, 30, and $40^{\circ}C$) after 5 hour seed imbibition into 50 ppm benzyladenopurine (BA) solution on growth and morphological characters of mungbean (cv. Keumseongnogdu, Owoolnogdu and Zhong Lu 1) sprouts. On the 6th day, the mungbean sprouts were sorted by 4 categories on the base of hypocotyl lengths; > 7cm, 4 to 7cm, < 4cm, and non-germination to calculate their composition rates, number of lateral roots, lengths of hypocotyl and root, diameters at middle and upper parts of hypocotyl, fraction fresh and dry weights were measured. AP more affected growth of the cultivars than AT showing little effect on them. In the composition rate of the above 4 categories, cv. Zhong Lu 1 had the highest rate in longer than 7cm but nearly the same rate in AP treatments. Rates of longer than 4cm hypocotyls in cv. Keumseongnogdu, Owoolnogdu were increased with longer AP but their rates of shorter than 4cm showed reverse response to the former. Formation rate and number of lateral roots per sprout were decreased with longer AP, showing more severe decrement when delayed 3 to 4 hour AP. Upper part of hypocotyls and roots were more thickened and shortened in longer AP, respectively. Total fresh weights had no significant difference between AP treatments while hypocotyl fresh weights were increased with longer AP. It was concluded that in mungbean sprout culture aeration from BA treatment to the first watering permitted at least 4 hours.

• Korean Journal of Weed Science
• /
• v.7 no.3
• /
• pp.257-264
• /
• 1987

Effect of various physical and chemical treatments on dark germination of Oenothera lamarckiana seeds were primarily investigated to find out factors affecting germination. Germination of seeds which did not germinate in the constant temperature and darkness was induced by several physical treatments such as sonification, wetting and drying, freezing and thawing, and removal of seed coat. Pretreatment of chilling ($4^{\circ}C$), freezing ($-10^{\circ}C$) and incubation at high temperature ($80^{\circ}C$) induced dark germination of seeds which did not germinate in the constant temperature ranges of 15 to $40^{\circ}C$ under darkness. Alternating temperature also had a stimulatory effect on dark germination of Oenothera lamarckiana seeds. Sensing of seeds to alternating temperature appeared to be completed during the first two days after imbibition. The minimum difference of temperature required for dark germination was $5^{\circ}C$ in the range of $15-25^{\circ}C$. A thiourea (1.0%) treatment induced dark germination, but GA, IAA, BA and Ethrel failed to do so.

• Korean Journal of Soil Science and Fertilizer
• /
• v.37 no.1
• /
• pp.41-45
• /
• 2004

A phosphate solubilizig bacterium, Pantoea agglomerans, was isolated from rhizosphere soils collected from Chungbuk area. A greenhouse experiment was conducted to study the effect of combined application of rock phosphate and P. agglomerans inoculation on plant growth and phosphate accumulation of rice (Oryza sativa L.). Apart from control that received no inputs, six treatments were planned as follows; 1) seed bacterization, 2) free cell inoculation and 3) bacteria immobilized beads inoculation, individually and in combination with 1 and 2.5 g of rock phosphate per pot. The results showed that plant growth and phosphate uptake were significantly enhanced as a result of bacterial inoculation. Bacterial inoculation in the form of immobilized beads and 1 g of rock phosphate was found to affect positively the rice plant growth and phosphorus accumulation than other treatments. The available phosphate concentration of the pot mixture also found improved as a result of P. agglomerans inoculation. A positive correlation was observed between the phosphate concentration in the pot mixture and phosphate accumulation in plant.

• Korean Journal of Soil Science and Fertilizer
• /
• v.30 no.4
• /
• pp.370-376
• /
• 1997

Experiments were conducted to investigate the effects of chromium application level, soil pH change, soil topping, application of some metals and application of organic matter on the absorption of chromium by radish and its growth. The results of experiments are summarized as following. Application of chromium as potassium dichromate up to 50 mg/kg did not affect the germination of radish seed. Application of chromium above this level affected the germination abversely. The dry matter yield of and absorption of chromium by radish was not affected by the application of chromium up to 100 mg/kg. Increased in soil pH decreased the uptake of chromium by radish under all range of chromium application rates. Under acidic condition (pH 5-6), the increase in the application of chromium resulted in the increase in the uptake of chromium by radish and lowering of dry matter production of radish. However, under alkaline condition (pH 7-8), increased application of chromium did not affect the uptake of chromium and the dry matter yield of radish. The application of Zn, Fe and Cu, up to 100 mg/kg did not affect not affect the uptake of chromium and dry matter yield of radish. The topping of soil with untreated soil after application of chromium up to five cm, did not affect the chromium uptake of radish, the same treatments tended to increase the dry matter yield of radish. The application of organic fertilizer(obtained from local market) up to the amount equivalent to 3000 kg/ha, although increased the dry matter yield of radish, did not affect the uptake of chromium by radish significantly. All the treatments tested in this study did not affect the translocation of chromium between root and shoot of radish.

• Korean Journal of Environment and Ecology
• /
• v.7 no.1
• /
• pp.10-21
• /
• 1993

Artificial acid rain (pH 3.0, 4.0 and 5.0) and ground was treated on the seeded pots of 4 species to examine its effects on germination and survival rate, seedling growth and contact angles on needle surface. Artificial acid rain was prepared by diluting sulfuric acid with ground water and ground water (pH 6.5) was used as control. Artificial acid rain was sprayed to seeded pots two or three times per week for growing season, one times per week for winter seasons. About 5mm of artificial acid rain was treated each time from early April, 1991 to early October, 1993. Germination and survival rate, soil acidity, seedling growth and contact angles on the needle surface were measured and compared among the treatments. The results were summarized as follows ; Artificial acid rain might have positive effects on growth-related characteristics of the seedlings in the first and second year of acid rain treatment, and the effects were differed among four species. All growth-related characteristics of the seedlings in third year, however, decreased with decrease of pH values of artificial acid rain. This was considered to the results of acidic accumulation over soil buffer capacity. Needle injury and biomass (defoliation) was correlated with the pH values of artificial acid rain, and this character might be a good criteria for early diagnosis of acid rain injury. The differences of soil acidity were significant among the treatments for all species. Contact angles between needle surface and water droplet decreased with decrease of pH values of artificial acid rain. Measuring and comparing contact angles might be very good criteria for early diagnosis of acid rain injury.

• Asian-Australasian Journal of Animal Sciences
• /
• v.15 no.10
• /
• pp.1433-1438
• /
• 2002

The study was carried out to evaluate leucaena seeds as a protein replacement of mustard seed cake (MSC) in the concentrate mixture of growing lambs. Fifteen owing male lambs (Local${\times}$Corridale) with an average body weight of 16.3 kg were allocated into three dietary treatments (T1, T2, and T3) with five animals in each group. Animals were offered dry mixed grass, berseem hay and concentrate mixture to meet their nutrient requirements. In concentrate mixture of T1, (Control) MSC was used as protein source, while in T2 and T3 groups, 25 and 50% of MSC was replaced by leucaena leucocephala seeds. On completion of three months (90 days) of feeding, a digestion cum-metabolism trial was conducted to determine DMI, nutrient utilization, and nitrogen balance. Changes in body weight were recorded at 15 day internals and eating patterns were recorded for 3 consecutive days at the end of the feeding trial. MSC had higher CP contents than leucaena seeds (27.0%). Mimosine contents in leucaena seeds were 1.1 compared to 0.2 and 0.4% in concentrate mixture of T2 and T3 group, respectively. Dry matter intake varied non-significantly ($79.3{\pm}1.2$ to $83.4{\pm}1.3g/kg$ $w^{0.75}$) across the dietary treatments. Digestibility of DM and cell wall polysaccharides (NDF, ADF. Cellulose and hemicellulose) were comparable, however CP digestibility was relatively lower in leucaena luecocephala seeds based groups (T2 $45.5{\pm}1.7$ and T3 $46.7{\pm}3.5$) compared to MSC supplemented group (T1 $47.7{\pm}0.9%$). The growth rate of lambs was non-significantly higher in T1 ($79.2{\pm}5.4$) compared to T2 ($73.8{\pm}8.8$) and T3 ($73.9{\pm}7.0$), respectively. The animals were in positive nitrogen balance and N-balance varied from 1.8 to 2.9 g/d across treatment groups. The eating rate (% of total offered) of concentrate up-to 15 min was relatively higher in T1 (82.4) than T2 (74.2) and T3 (77.8%). However no effect of leucaena seeds was recorded on total DMI of animals. The results of the study revealed that the inclusion of up to 50% leucaena seeds, as protein source in concentrate mixture of lambs had no adverse effect on DMI, nutrient utilization, eating patterns, nitrogen balance and growth performance of lambs.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2004.09a
• /
• pp.37-47
• /
• 2004

In Korea, over three hundreds of the coal mines were closed or abandoned due to the depression of the mining industry since the late 1980s. Many of them locate in the steep mountain valleys and the coal mine wastes had been disposed without a proper treatment From these mines, enormous amounts of coal mine overburdens have been abandoned in the slopes and the ample amounts of acid mine drainage (AMD) from either portal or overburdens have been discharging directly to the streams, causing the detrimental effects on soil and water qualities. Objectives of this research were to reclaim the coal mine overburdens using the lime waste cake from the soda ash production by stabilizing the overburden slopes, introducing the vegetation alleviate the environmental problems caused by the closed coal mines. The percentages of the grass distribution ratio (%) and the surface coverage ($\textrm{cm}^2$) in each treatment plot were determined during June to August after seed spraying grasses such as orchard grass (Dactylis glomerata L), Kentucky Bluegrass (Poa pratensis L.) and Eulalia (Miscanthus sinensis Anderss) at the end of May. The grasses covered only 15.5 % of the coal overburden plot at the early stage but the coverage was increased with time to 33% in August. Growth of such grasses was enhanced with the combined treatments of lime waste and topsoil resulting in the increased surface coverage by the grasses. The Increment of the surface coverage from June to August was higher with lime waste treatments. The distribution percentages and surface coverage were highest when the lime wastes were treated at 25 % of the lime requirement. This might be related with the high salt contents in the hire wastes. Results demonstrated that the amounts of lime wastes at 25% of the lime requirement were sufficient for neutralizing the acidic coal overburden and introducing the re-vegetation. Either layering between the coal waste and topsoil or mixing with coal overburdens could be adopted as the lime waste treatment method. The combined treatment of lime wastes and topsoil was recommended for re-vegetation in the coal overburden slopes. The lime wastes from the soda ash production might have a potential to be recycled for the reclamation of the abandoned coal mines to alleviate the environmental problems associated with coal mine waste.

• Korean Journal of Soil Science and Fertilizer
• /
• v.25 no.1
• /
• pp.77-88
• /
• 1992

Effective strains of cowpea bradyrhizobia JB7 $nal^rspe^r$ and CB756 $str^rrif^r$, antibiotic-resistant variants of JB7 and CB756, respectively, were used to examine changes of rhizosphere populations and nodule occupancy. Populations of each strain increased gradually in the rhizosphere, reaching a maximum of about $10^8$ cells per root system. Nodule number increased as the density of inoculum increased from $10^2$ cells to $10^8$ cells per seed. Inoculation with liquid suspension resulted in the formation of more nodules than the peat slurry or granule inoculation. When JB7 $nal^rspe^r$ and CB756 $str^rrif^r$ were introduced in equal numbers in inoculum mixtures the former consistantly occupied the majority of nodules with all three groundnut cultivars used. There was no difference in yield between nitrogen treatments and inocultation treatments.