• 한국작물학회지
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• 제54권4호
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• pp.346-350
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• 2009

The cultivated radish (Raphanus sativus L.) is a major vegetable crop in the world wide and fast-growing species that grows inhabitats of six continents. It is very important to determine hybrid seed purity in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in $F_2$-hybrid radish cultivars demonstrated. One hundred eighty seeds from the F1 male and female harvest were subsequently screened for seed purity using 13 primers. The 13 primers result in 17 cultivar-specific bands and 23 variable RAPD bands scored for cultivar. RAPD analysis of hybrid seeds from the harvest revealed 128 seeds tested except underdevelopment and decayed seeds were sibs. Especially, $F_2$ hybrids of radish, OPC13, OPD20 were presented clear hybrid bands. It maintains higher than average level of genetic diversity compared with their correspondent parents. RAPD amplification of DNA extracted from germinated individuals from the female harvest reveal that 10 of 208 seeds tested were self-inbred (4.8%). RAPD analysis of hybrid seeds from the male harvest revealed 7 of the 208 seeds tested were sibs (3.4%). The RAPD may lead to a better insight in to the hybrid seed purity.

• 한국약용작물학회지
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• 제17권3호
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• pp.198-203
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• 2009

Isatis tinctoria var. yezoensis (Ohwi) Ohwi (Cruciferae) is one of major natural dyeing crops in the world and also have used as a medicinal plant in Korea. We evaluated seed purity in $F_1$-hybrid accessions using amplified fragment length polymorphism (AFLP) markers. One hundred sixty seeds from the male and female harvests were subsequently screened for seed purity with ten primers. The 13 accession-specific bands and many variable AFLP bands scored for accessions. Especially, E-AAC/M-CAA and E-AAG/M-CAT were presented clear hybrid bands for $F_1$ hybrids. $F_1$ hybrids maintained higher average level of genetic diversity compared with their correspondent parents. Self-inbred seeds from the female and male harvests were revealed 8.0% and 5.0%, respectively. The AFLP may lead to a better insight in to the hybrid seed purity test in I. tinctoria var. yezoensis.

• 한국세라믹학회지
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• 제35권8호
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• pp.878-884
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• 1998

Synthesis of high purity mullite from korean resourceful kaolin and water-soluble aluminium salts was in-vestigated by addition of mullite seed. Single phase of mullite was formed at 1350$^{\circ}C$ however its mullite con-tent was 31-33% Maximum content of mullite synthesized from kaoin aluminium nitrate and 8wt% mul-lite seed was 98% at 1600$^{\circ}C$ for 4 hours.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.46.2-46.2
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• 2007

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• International Journal of Computer Science & Network Security
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• 제22권10호
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• pp.271-281
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• 2022

Manual seed classification challenges can be overcome using a reliable and autonomous seed purity identification and classification technique. It is a highly practical and commercially important requirement of the agricultural industry. Researchers can create a new data mining method with improved accuracy using current machine learning and artificial intelligence approaches. Seed classification can help with quality making, seed quality controller, and impurity identification. Seeds have traditionally been classified based on characteristics such as colour, shape, and texture. Generally, this is done by experts by visually examining each model, which is a very time-consuming and tedious task. This approach is simple to automate, making seed sorting far more efficient than manually inspecting them. Computer vision technologies based on machine learning (ML), symmetry, and, more specifically, convolutional neural networks (CNNs) have been widely used in related fields, resulting in greater labour efficiency in many cases. To sort a sample of 3000 seeds, KNN, SVM, CNN and CNN-SVM hybrid classification algorithms were used. A model that uses advanced deep learning techniques to categorise some well-known seeds is included in the proposed hybrid system. In most cases, the CNN-SVM model outperformed the comparable SVM and CNN models, demonstrating the effectiveness of utilising CNN-SVM to evaluate data. The findings of this research revealed that CNN-SVM could be used to analyse data with promising results. Future study should look into more seed kinds to expand the use of CNN-SVMs in data processing.

• 생명과학회지
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• 제18권7호
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• pp.907-911
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• 2008

무(Raphanus sativus L.)는 세계적으로 중요한 작물 중의 하나이다. 십자화과 식물 종자 생산에서 원하지 않은 내교잡에 의한 종자 결실로 오염이 발생하므로 씨의 순수성 검증은 매우 중요하다. 재배종 진주 대평 무(R. sativus cv. Daepeng)와 백자 무(R. sativus cv. Backza)의 교잡 분석을 실시하였다. 알로자임으로 상업적으로 이용되는 잡종 제1세대($F_1$) 무에 있어서 씨의 순수성을 평가하였다. 웅성과 자성 양친 360개체에 27개 대립유전자좌위를 조사하였다. 특히 Par-1 ($aa{\times}bb$), Lap-1($aa{\times}bb$), Est-1 ($aa{\times}bb$)에서 명확한 잡종 밴드를 나타내었다. Est-1 대립유전자좌위에서 자성 배우체로부터 기원된 것이 15개체(8.3%)가 발견되었고, 웅성 배우체로부터 기원된 것이 26개체(14.4%)가 발견되었다. 또한 다양도 측면에서 양친 계통에 비해 잡종 계통에서 높은 유전적 다양도를 유지하고 있었다. 샤논의 정보지수(Shannon's index)를 이용한 표현형 다양도는 교잡 계통이 가장 높았다. 알로자임에 의한 무 계통의 교잡에 의한 종자 생성에서 씨에 대한 순수성 검증이 효과적으로 탐지되어 육종 연구에 기여할 수 있을 것으로 판단된다.

• 한국산림과학회지
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• 제95권5호
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• pp.621-627
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• 2006

This study was conducted to understand the pattern and characteristics of seed rain in the broadleaved-Korean pine mixed forest. We established 287 $0.5m^2$ circular seed traps and collected and identified fallen seeds in the traps every two weeks in $150m{\times}150m$ sample plot of the permanent nine hectare of experimental plot in 2005. The overall average density of seed rain was $864.2/m^2$. The seeds of Betula costata Betula costata had the highest number of fallen seeds as $676.0/m^2$ (78.2%), followed by Abies nephrolepis as $57.5/m^2$, B. platyphylla as $37.9/m^2$, Tilia amurensis as $32.2/m^2$, Acer ukurunduense as $17.0/m^2$, A. tegmentosum $14.8/m^2$, and so on. Pinus koraiensis was recorded only $2.5/m^2$ of fallen seeds mainly owing of Korean pine had low rate of purity due to the animal and microbiological predation. Most of seed dispersal have started from the middle to late August and come to an end on the middle of November. The peak time of seed dispersal varied depending on the species. The rate of pure seed by dispersal time varied according to the species, thereupon the aspect of predation and the rate of blasted seed which had influence on the rate of purity also varied according to the species. The density of Korean pine seed rain in the forest gap was significantly different at $P{\leq}0.05$ from in the closed canopy. But the other species had no difference among canopy coverage.

• 대한본초학회지
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• 제25권1호
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• pp.45-54
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• 2010

Objectives : The purpose of this study is to determine the impurity tolerance of Zanthoxylum Peel. Methods : Compare with medicinal Herb Books and the Pharmacopoeia of 6 nations. Results : Current Pharmacopoeia show different Zanthoxylum Peel's Purity, such as 2.0% of seeds and fruit stalk, etc. in North Korea, 2% of seeds in Vietnam, totally 3% in China. On the other hand, Korea and Japan set the total number 26.0% including the specific numbers such as 20.0% of seeds, 5.0% of fruit stalk, 1.0% of the other foreign matter. This Zanthoxylum Peel's Purity, 26.0%, is too high compared to that of other medical matters specified by The Korean Pharmacopoeia Ninth Edition. When The Japanese Pharmacopoeia Sixth Edition firstly set the Zanthoxylum Peel's Purity, the herbal name was Fructus. However, since the part for medical usage in origin is well-ripen pericarp, not seed, the permissible level, 30.0%, is supposed to be simple error range, 3.0%. Conclusions : As a result, I think bills concerning the Zanthoxylum Peel's Purity should be revised to the total number 3.0% or specifically set the level 2.0% of seeds, 1.0% of fruit stalk, twig and so on.

• 원예과학기술지
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• 제32권4호
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• pp.525-534
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• 2014

Microsatellite 표지를 이용하여 국내에서 시판되고 있는 멜론 58품종의 식별과 멜론 육종 계통 '10H08'을 이용하여 $F_1$ 종자 순도를 평가하였다. 412개의 microsatellite 표지 중 다형성 정도가 높은 29개는 품종 그룹 내에서도 다양한 유전 변이를 나타내었으며 분자표지의 유전자형에 의해 모든 품종을 식별할 수 있었다. Microsatellite 표지의 대립유전자를 이용하여 멜론 58품종에 대한 계통도를 작성하였을 때 멜론의 형태적 특성과 일치하면서 2개의 대그룹으로 구분되었다. $F_1$ 종자의 순도 검정에 microsatellite 표지를 활용하기 위하여 29개의 표지를 '10H08' 계통의 양친에 대하여 검정하였을 때 5개의 프라이머가 다형성을 보였으며, 이 중 한 개의 프라이머 'CMGAN12'는 양친간에 뚜렷한 다형성 밴드를 나타내었다. 이 프라이머를 192개의 $F_1$ 종자에 대하여 검정하였을 때 자식주로 보이는 개체가 분석된 종자 내에서 명확하게 구분되었다. 본 연구 결과에서 선정된 멜론 품종 식별용 microsatellite 표지는 멜론 품종의 지문화뿐만 아니라 $F_1$ 종자의 순도 검정이 가능하여 종자회사에서 매우 유용하게 활용될 수 있는 것으로 나타났다.

• 멤브레인
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• 제26권5호
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• pp.381-390
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• 2016

본 연구에서는 고순도의 모데나이트(Mordenite) 입자를 합성하기 위하여 천연 제올라이트를 시드로 사용하여 시드의 농도 및 수열합성 시간에 따른 천연 제올라이트 시드가 합성에 미치는 영향을 고찰하였다. 그 결과 시드가 입자의 형성에 큰 영향을 끼치는 것을 확인할 수 있었고 시드를 3 g/100 g batch 주입하여 $140^{\circ}C$에서 72시간 동안 수열합성을 진행하였을 때 $1-2{\mu}m$ 사이즈의 고순도 모데나이트 입자를 합성할 수 있었다. 이를 통해 모데나이트 입자의 성장 기구를 규명할 수 있었으며, 모데나이트 입자 형성에 있어 시드는 첫째, 구형 모데나이트 전구체 형성 자리 공급의 역할과, 둘째 모데나이트 원료 물질 소스 역할을 한다는 것을 알 수 있었다. 합성된 모데나이트 입자의 가스 흡착량 분석 결과 $CO_2$ 기체의 흡착량이 97.19 mg/g로 다른 가스들에 비해 비교적 높은 흡착성능을 보였으며, $CO_2/H_2$의 선택도가 가장 우수한 것으로 나타났다. 따라서 이러한 결과들을 바탕으로 용도에 맞는 고순도 상의 모데나이트 입자를 합성할 수 있음을 확인하였고 보다 낮은 가격으로 우수한 분리성능을 갖는 분리막 소재개발에 활용할 수 있을 것이라 판단된다.