• Title/Summary/Keyword: Seed point

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Development of genetic transformation method of Korean soybean (국내콩 형질전환 기술개발)

  • Jeon, Eun-Hee;Chung, Young-Soo
    • Journal of Plant Biotechnology
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    • v.36 no.4
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    • pp.344-351
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    • 2009
  • Current status of soybean transformation method in Korera was reviewed with recent publications. Most frequently used method for genetic transformation was Agrobacterium-mediated transformation on cotyledonary node which is most popular method used in foreign country. In addition to this, various methods such as sonicationmediated transformation, in planta transformation, and transformation on meristem tissue of germinating seed, have been tried in Korea, even though their efficiencies on repeatability and stability were relatively low. Based on the promising results developed recently by reviewer, several important considerations for successful soybean transformations were suggested. They are 1) proper genotype screening, 2) targeting transformation on exact point, 3) multiple shoot formation, 4) efficient selection pressure, 5) successful shoot elongation, 6) efficient root formation. These are the basic requirements for stable and highly efficient soybean transformation of Korean soybean.

Fertility and Some Agronomic Characteristics of ′Sawonppong26′ Autotetraploid Artificially derived from Keomseolppong (Morus spp) (검설뽕의 동질4배체 ′4원뽕26호′의 임성 및 재배학적 특성)

  • 박광준;이용기
    • Journal of Sericultural and Entomological Science
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    • v.42 no.2
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    • pp.67-72
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    • 2000
  • Autotetraploid 'Sawonppong 26'was induced by dropping clochicine solution in growing point of Keomseolppong which is of high rootability and some agronomic charactelistics are as follows. Sawonppong 26 is a tetraploid of Keomseolppong with 56 of the chromosome number. Its fertlization percent was 59.4% and the germination percent of sank seed was normal. Sugar degree of mulberry fruit was 13.3% and total acidity was 0.69%. Single fruit weight of Sawonppong 26 was increased by 79%, as compared to that of Keomseolppong. Even though percent of survival of hard wood cutting was 94%, number of rootings was less and diameter of root was slender, it is expectedly a promising variety to be used for a breeding parent from the aspect of rootability.

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The matching algorithm with the satellite images using a dynamic triangular image warping method (동적 삼각형 영상 왜곡 보상 방법을 이용한 위성 영상 정합 알고리듬)

  • Jeon, Byung-Min;Lee, Heung-Jae;Lee, Kwae-Hi
    • Proceedings of the KIEE Conference
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    • 1998.07g
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    • pp.2209-2211
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    • 1998
  • This paper presents the matching algorithm with the satellite images using the image warping method. Two stereo images, which are used for the DEM(Digital Elevation Model) extraction, are generally distorted because the images are acquired at different locations and angles. Therefore, the matching Process can't be executed with the original images. To solve this problem, a dynamic triangular image warping method is proposed. At first, the initial matching is executed with seed point, and then, using the matched points from the initial matching, the distorted images is compensated. We experimented this algorithm with the parts of the $6000{\times}6000$ SPOT satellite images. The experiment results show this algorithm is superior to other warping algorithm.

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Regulation Mechanism of Soybean Storage Protein Gene Expression (대두 저장단백질 유전자의 발현 조절 메카니즘)

  • 최양도;김정호
    • Proceedings of the Botanical Society of Korea Conference
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    • 1987.07a
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    • pp.283-307
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    • 1987
  • Glycinin and $\beta$-conglycinin are the most abundant storage protein in soybean. These proteins are known to be synthesized predominantly during germination and cell expansion phase of seed development for short period, and synthesized not in other tissues. Genes encoding these storage proteins are useful system to study the mechanism of development stage and tissue specific gene expression in eukaryotes, especially plants, at the molecular level. The cDNA and genomic clones coding for glycinin have been isolated and regulation mechanism of the gene expression has been studied. Initially, development and tissue-specific expression of the glycinin gene is regulated at the level of transcription. Post-transcriptional processing is also responsible for delayed accumulation of the mRNA. Translational control of the storage protein gene has not been reported. Post-translational modification is another strategic point to regulate the expression of the gene. It is possible to identify positive and/or negative reguratory clements in vivo by producing transgenic plants agter gene manipulation. Elucidation of activation and repression mechanism of soybean storage protein genes will contribute to the understanding of the other plant and eukaryotic genes at molecular level.

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Feature Points Detection based on R2-map for Efficiency Liver Recognition (효율적인 간 인식을 위한 R2-map 기반의 특징점 검출 방법)

  • Eun, Sung-Jong;WhangBo, Taeg-Keun
    • Proceedings of the Korean Information Science Society Conference
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    • 2012.06c
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    • pp.385-387
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    • 2012
  • MR 영상에서 간의 인식은 간에 존재하는 질병을 파악하는 것뿐만 아니라 간에 대한 치료 방법이나 수술방법을 결정하는 중요한 정보를 제공한다. 이러한 일반적인 간의 인식 방법은 영역 분할 알고리즘을 기반으로 처리되어진다. IT분야에서의 영역 분할 알고리즘은 대부분 밝기 정보, 형태 정보, 패턴 분석 등 다양한 입력 정보의 컴퓨팅 처리를 통해 처리되어 진다. 그러나 이러한 컴퓨팅 방법으로는 앞서 언급된 입력정보들이 의미가 없을 경우, 영역 분할에 많은 제약이 따르게 된다. 따라서 본 논문은 이러한 컴퓨팅 처리의 근본적인 제약사항을 해결하고자, MR 이론의 R2-map정보 기반의 효과적인 영역 분할 방법은 제안하였다. 본 방법은 간 영역이 포함된 영상에서 실험하였으며, R2-map맵의 일부 특징점을 Region growing의 Seed point로 설정하여 경계가 모호하더라도 영역 분할이 가능하게끔 하였다. 해당 영상의 실험 결과 8.5%의 평균 오차로 일반적인 영역 분할 알고리즘에 비해 높은 정확도가 산출되었다.

Quantification of Fibers through Automatic Fiber Reconstruction from 3D Fluorescence Confocal Images

  • Park, Doyoung
    • Journal of Advanced Information Technology and Convergence
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    • v.10 no.1
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    • pp.25-36
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    • 2020
  • Motivation: Fibers as the extracellular filamentous structures determine the shape of the cytoskeletal structures. Their characterization and reconstruction from a 3D cellular image represent very useful quantitative information at the cellular level. In this paper, we presented a novel automatic method to extract fiber diameter distribution through a pipeline to reconstruct fibers from 3D fluorescence confocal images. The pipeline is composed of four steps: segmentation, skeletonization, template fitting and fiber tracking. Segmentation of fiber is achieved by defining an energy based on tensor voting framework. After skeletonizing segmented fibers, we fit a template for each seed point. Then, the fiber tracking step reconstructs fibers by finding the best match of the next fiber segment from the previous template. Thus, we define a fiber as a set of templates, based on which we calculate a diameter distribution of fibers.

Ultrastructure of Vascular Meristems in the Rhizome of Botrychium ternatum (고사리삼 지하경의 유관속 분열조직 미세구조)

  • 소웅영
    • Journal of Plant Biology
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    • v.36 no.4
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    • pp.357-362
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    • 1993
  • To elucidate the origin of secondary growth in the rhizome of B. ternaturn, the developmental changes of vascular cambium was observed in ultrastructural features. The vascular cambium was gradually differentiated from procambium as in seed plants, but the cambial activity did not persist very long so that the cambial cells became a dormant state like fossil cryptogams. Dense cytoplasm of procambial cells became progressively sparse during the growth, and the tiny vesicles were fused to form numerous small vacuoles and then a few large vacuoles. These gradual changes and the occurrence of storage materials which was associated with the developmental stages might support the progressive differentiation of the cambial cells. In addition, the cessation of cambial activity could be indicated by the facts that late vascular cambial cells accumulate large lipid bodies and show very small peripheral cytoplasm and unlikely thickened cell wall, compared to other meristematic cells. Therefore. the vascular cambium showed the characteristics of both seed plants and fossil cryptogams from the view point of cambial ontogeny and activity.tivity.

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Microstructures and Mechanical Properties of SiC Containing $SiC_{platelet}$ Seeds of Various Size (여러 가지 크기의 $SiC_{platelet}$ Seed를 함유한 SiC 세라믹스의 미세구조 및 기계적 특성)

  • Choi, Myoung-Je;Park, Chan;Park, Dong-Soo;Kim, Hai-Doo
    • Journal of the Korean Ceramic Society
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    • v.36 no.10
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    • pp.1094-1101
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    • 1999
  • Liquid phase sintered silicon carbides were obtained by sintering of $\alpha$-SiC and $\beta$-SiC powders as starting materials at 2173K and 2273K respectively. The SiCplatelet seeds of different sizes were obtained by a repeated ball milling and sedimentation. Their mean size (d50) were 2.217 ${\mu}{\textrm}{m}$ 13.67 ${\mu}{\textrm}{m}$, 22.17${\mu}{\textrm}{m}$ respectively 6wt%Al2O3-4 wt% Y2O3 was used as the sintering additives for the liquid phase sintering. The two silicon carbides had a bimodal microstructure consisting of small matrix grains and large platelike grains when the SiCplatelet seeds were added. In the case of the $\beta$-SiC the appreciable phase transformation occurred as sintering temperature increased from 2173K to 2273K and resulted in matrix shape change from equiaxed into platelike grains. In contrast there was no shape change for the $\alpha$-SiC. The size of large grains in the $\alpha$-SiC of large grains in the $\alpha$-SiC was larger than that of the large grains in the $\beta$-SiC These results suggested that the growth of the $\alpha$-SiCplatelet in the $\alpha$-SiC matrix was more favored than that of the $\alpha$-SiCplatelet in the $\beta$-SiC matix. The three point flexural strength decreased as the added seed size increased. Fracture toughness values of samples sintered at 2273K were higher than those of samples sintered at 2173K.

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Image Segmentation based on Statistics of Sequential Frame Imagery of a Static Scene (정지장면의 연속 프레임 영상 간 통계에 기반한 영상분할)

  • Seo, Su-Young;Ko, In-Chul
    • Spatial Information Research
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    • v.18 no.3
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    • pp.73-83
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    • 2010
  • This study presents a method to segment an image, employing the statistics observed at each pixel location across sequential frame images. In the acquisition and analysis of spatial information, utilization of digital image processing technique has very important implications. Various image segmentation techniques have been presented to distinguish the area of digital images. In this study, based on the analysis of the spectroscopic characteristics of sequential frame images that had been previously researched, an image segmentation method was proposed by using the randomness occurring among a sequence of frame images for a same scene. First of all, we computed the mean and standard deviation values at each pixel and found reliable pixels to determine seed points using their standard deviation value. For segmenting an image into individual regions, we conducted region growing based on a T-test between reference and candidate sample sets. A comparative analysis was conducted to assure the performance of the proposed method with reference to a previous method. From a set of experimental results, it is confirmed that the proposed method using a sequence of frame images segments a scene better than a method using a single frame image.

Effects of Inoculation of Rhizobium and Arbuscular Mycorrhiza, Poultry litter, Nitrogen, and Phosphorus on Growth and Yield in Chickpea

  • Solaiman A. R. M.;Rabbani M. G.;Molla M. N.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.4
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    • pp.256-261
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    • 2005
  • The experiment was conducted at the Ban­gabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur to study the response of chickpea (Cicer arietinum L) to dual inoculation of Rhizobium and arbuscular mycorrhiza, poultry litter, nitrogen, and phosphorus on spore population and colonization, nodulation, growth, yield attributes, and yield. The performance of Rhizobium inoculant alone was superior to control in all the parameters of the crop studied. Among the treatments dual inoculation of Rhizobium and arbuscular mycorrhiza in presence of poultry litter performed best in recording number and dry weight of nodules, dry weight of shoots and roots, number of pods/plant, number of seeds/pod, and seed yields of chickpea. The highest seed yield of 3.96g/plant was obtained by inoculating chickpea plants with dual inoculation of Rhizobium and arbuscular mycorrhiza in association with poultry litter. Treatments receiving dual inoculation of Rhizobium and arbuscular mycorrhiza in presence of nitrogen and phosphorus, Rhizobium inoculant in presence of nitrogen and phosphorus, and that of arbuscular mycorrhiza in presence of nitrogen and phosphorus were similar as that of treatment receiving dual inoculation of Rhizobium and arbuscular mycorrhiza in presence of poultry litter. From the view point of nodulation, growth, yield attributes, and yields of chickpea, dual inoculation of Rhizobium inoculant and arbuscular mycorrhiza along with poultry litter was considered to be the balanced combination of nutrients for achieving the maximum output from cultivation of chickpea in Shallow Red Brown Terrace Soil of Bangladesh.