• Title/Summary/Keyword: Seed grain

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Proteomic Analysis of Drought Stress-Responsive Proteins in Rice Endosperm Affecting Grain Quality

  • Mushtaq, Roohi;Katiyar, Sanjay;Bennett, John
    • Journal of Crop Science and Biotechnology
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    • v.11 no.4
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    • pp.227-232
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    • 2008
  • Drought stress is one of the major abiotic stresses in agriculture worldwide. We report here a proteomic approach to investigate the impact of post-fertilization drought on grain quality in rice seed endosperm (Oryza sativa cv. IR-64). Plants were stressed for 4 days at 3 days before heading. Total proteins of endosperm were extracted and separated by two-dimensional gel electrophoresis. Not many protein spots showed differential accumulation in drought-stressed samples. More than 400 protein spots were reproducibly detected, including three that were up-regulated and five down-regulated. Mass spectrometry analysis and database searching helped us to identify six spots representing different proteins. Functionally, the identified proteins were related to protein synthesis and carbohydrate metabolism, such as Granule-Bound Starch Synthase (GBSS, Wx protein), which is thought to play a very important role in starch biosynthesis and quality, a very crucial factor in determining rice grain quality.

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Patterns between wall pressures and stresses with grain moisture on cylindrical silo

  • Kibar, Hakan
    • Structural Engineering and Mechanics
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    • v.62 no.4
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    • pp.487-496
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    • 2017
  • The focus of this study were to investigate patterns between wall pressures and stresses with grain moisture of soybean and rice varieties widespread cultivated in Turkey in order to determine needed designing parameters for structure analysis in silos at filling and discharge. In this study, the wall pressures and stresses were evaluated as a function of moisture contents in the range of 8-14% and 10-14% d.b. The pressures and von Mises stresses affected as significant by the change of grain moisture content. The main cause of pressure and stress drops is changed in bulk density. Therefore is extremely important bulk density and moisture content of the product at the structural design of the silos. 4 mm wall thickness, were determined to be safe for von Mises stresses in both soybean and rice silos is smaller than 188000 kPa.

Effect of $\alpha-SiC $seed on microstructure and fracture toughness of pressureless-sintered $\beta-SiC$ ($\alpha-SiC $seed의 첨가가 상압소결된 $\beta-SiC$의 미세구조와 파괴인성에 미치는 영향)

  • Young-Wook Kim;Won-Joong Kim;Kyeong-Sik Cho;Heon -Jin Choi
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.7 no.1
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    • pp.18-26
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    • 1997
  • $\beta-SiC $powder with or without the addition of 1 wt% of $\alpha-SiC$ particles (seeds) was pressureless-sintered at $1950^{\circ}C$ for 0.5, 2 and 4 h using $Y_3Al_5O_{12}$ (yttrium aluminum garnet, YAG) as a sintering aid. The introduction of $\alpha-SiC$ seeds into $\beta-SiC$ accelerated :he grain growth of elongated large grains during sintering, resulting in the coarser microstructure. The fracture toughnesses of materials with $\alpha$-SiC seeds and without $\alpha-SiC$ seeds sintered for 4 h were 7.5 and 6.1 $MPa\cdot \textrm m^{1/2}$, respectively. Higher fracture toughness of the material with seeds was due to the enhanced bridging by elongated grains, resulting from coarser microstructure.

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Deep Learning-based Rice Seed Segmentation for Phynotyping (표현체 연구를 위한 심화학습 기반 벼 종자 분할)

  • Jeong, Yu Seok;Lee, Hong Ro;Baek, Jeong Ho;Kim, Kyung Hwan;Chung, Young Suk;Lee, Chang Woo
    • Journal of Korea Society of Industrial Information Systems
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    • v.25 no.5
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    • pp.23-29
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    • 2020
  • The National Institute of Agricultural Sciences of the Rural Developement Administration (NAS, RDA) is conducting various studies on various crops, such as monitoring the cultivation environment and analyzing harvested seeds for high-throughput phenotyping. In this paper, we propose a deep learning-based rice seed segmentation method to analyze the seeds of various crops owned by the NAS. Using Mask-RCNN deep learning model, we perform the rice seed segmentation from manually taken images under specific environment (constant lighting, white background) for analyzing the seed characteristics. For this purpose, we perform the parameter tuning process of the Mask-RCNN model. By the proposed method, the results of the test on seed object detection showed that the accuracy was 82% for rice stem image and 97% for rice grain image, respectively. As a future study, we are planning to researches of more reliable seeds extraction from cluttered seed images by a deep learning-based approach and selection of high-throughput phenotype through precise data analysis such as length, width, and thickness from the detected seed objects.

Phenotypic Characterization of Amaranth Resources for the Selection of Promising Materials

  • Hwang Bae Sohn;Su Jeong Kim;Jung Hwan Nam;Do Yeon Kim;Jong Nam Lee;Su Young Hong;Yul Ho Kim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.211-211
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    • 2022
  • Amaranth is a nutritious and broadly adapted seed crop in high demand around the world. A preliminary approach for understanding the genetics of amaranth resources entails a morphologic characterization, which can provide the basis for breeding the first variety in Korea, leading to satisfying the needs of farmers and consumers. Therefore, this study aimed to evaluate the phenotypic characteristics of ten genetic amaranth accessions for the selection of outstanding accessions in terms of yield and grain quality. A randomized complete block design was used, with fifteen replications for each accession under field conditions. Five quantitative and three qualitative descriptors were evaluated with descriptive analysis. The results showed that the accessions with plant heights smaller than the average (>112.7 cm) presented lower yields and smaller seed sizes, thus decreasing the grain quality. The cluster analyses established groups of accessions with good yields (>30.1 g of seeds per plant) and stable morphological characteristics. Based on yield and morphological descriptors, the proposed selection index indicated four accessions as potential parents for amaranth breeding programs in Kora.

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Effects of Seed Size Variation on Germination and Seeding Vigour of Sorghum (Sorghum bicolor L.) (수수의 종자크기가 종자활력과 출아에 미치는 영향)

  • Jung, Ki-Yuol;Yun, Eul-Soo;Park, Chang-Young;Choi, Young-Dae;Hwang, Jae-Bok;Jeon, Seung-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.3
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    • pp.219-225
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    • 2012
  • Seed size has been considered as an effective criteria for selection of the most vigorous seeds in sorghum [(Sorghum bicolor (L) Moench]. The smaller seeds were inferior to the larger sizes in emergence and grain yield. This study was conducted to determine germination rate, field emergence and vigor of sorghum for selection of high quality seed by different seed size. Sorghum cultivar of two (Hwanggeumchal and Tojong) were separated into five seed size proportion (<2.36, 2.80, 3.15, 3.35 and >3.55 mm diam.) according to seed size. The larger seed was more higher 1,000 seeds weight, seed density, carbohydrates and protein content. Total seed germination performing varied 92% at the largest size (>3.55 mm diam.) frequently inferior to slightly 67% at smaller seed (2.36 to 2.80 mm diam.) in the standard germination test. Seed size did have a significant effect on mean emergence time (MET) and maximum emergence rate index (ERI) and percentage of emergence. It should be noted that the results refer to MET of sorghum seeds ranging from 4.26 to 4.74 days. The relationship of seed size was not only to stand establishment but to grain yield. Yield was most affected by seed size and large seeds were superior to the smaller seed in 25~37% of the cases. Especially, yield was significant under 3.15 mm the beginning.

Single Cross Maize Hybrid 'Hwangdaok' for High Grain Yield (다수성 종실용 옥수수 단교잡 신품종 '황다옥')

  • Son, Beom-Young;Baek, Seong-Bum;Kim, Jung-Tae;Lee, Jin-Seok;Bae, Hwan-Hee;Go, Young-Sam;Kim, Sun-Lim
    • Korean Journal of Breeding Science
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    • v.51 no.2
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    • pp.105-109
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    • 2019
  • 'Hwangdaok', a new maize F1 hybrid (Zea mays L.), was developed by the maize breeding team at the National Institute of Crop Science (NICS), RDA in 2016. The high-yielding yellow dent hybrid named 'Hwangdaok' was obtained by crossing between two inbred lines, 'KS203' and 'KS190'. After advanced yield trial in Suwon in 2012, regional yield trial was subsequently carried out to evaluate the growth and yield of 'Hwangdaok' at three different locations from 2014 to 2016. The number of days to silking of 'Hwangdaok' is 76. The plant height is 262 cm and ear height ratio is 51%, which are similar to those of 'Jangdaok'. It has resistance to lodging. The number of ears per 100 plants is 95. The ear length is 21.3 cm and weight of 100 seeds is 34.7 g, similar to those of 'Jangdaok'. It has moderate resistance to southern leaf blight (Bipolaris maydis) and European corn borer (Ostrinia nubilalis). The grain yield (10 ton/ha) of 'Hwangdaok' is 6% higher than that of 'Jangdaok'. The seed production rate of 'Hwangdaok' is acceptable due to good synchronization of flowering period during crossing between the seed parent, KS203, and the pollen parent, KS190, in Yeongwol. F1 seed yield was 1.68 ton/ha. 'Hwangdaok' can be a suitable cultivar to plain areas in Korea. (Variety registration No. 7502).

Intra- and Inter-Variation of Protein Content in Soybean Cultivar Seonnogkong (선녹콩 개체간 및 개체내 단백질 함량 변이)

  • Im, Moo-Hyeog;Choung, Myoung-Gun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.spc
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    • pp.78-83
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    • 2008
  • Soybean [Glycine max (L.)] is a major source of protein for human and animal feed. Inter- and intra-genotype variation of soybean protein has been investigated by soybean researchers. However, limited sample amount of soybean single seed there is no report that investigated intra-plant variation of soybean protein within soybean plant. Recently a non-destructive NIR (near-infrared reflectance) spectroscopy using single seed grain to analyze seed protein was developed. The objectives of this study were to understand variation of seed protein content within plant and to determine the amount of minimum sample size which can represent protein content for a soybean plant. Frequency distribution of protein content within plant showed normal distribution. There was an intra-cultivar variation for protein content in soybean cultivar Seonnogkong. Difference of protein content among single plants of Seonnokong was recognized at 5% level. Seeds in lower position on plant stem tended to accumulate more protein than in higher position. There was significant difference for protein content between sample size 5 seeds and sample size of more than 5 seeds (10, 20, 30, 40, and 50 seeds) at a soybean plant with 57 seeds however no difference was recognized among sample size (5, 10, 20, and 30 seeds) at a soybean plant with 33 seeds. Around 20% seeds of soybean from single plant needed to determine the protein content to represent protein content of single soybean plant. This study is the first one to report evidence of intra-plant variation for proteincontent which detected by non-destructive NIR spectroscopy using single seed grain in soybean.

Quality Characteristics of Instant Gochujang added with Red Pepper Seeds Powder (고추씨를 첨가한 즉석 고추장의 품질특성)

  • Lee, Keum-Ok;Kim, Ki-Bbeum
    • Culinary science and hospitality research
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    • v.24 no.3
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    • pp.15-24
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    • 2018
  • This study produced instant Gochujang(red pepper paste) with enhanced convenience by increasing nutritional value and palatability, and by reducing production period, adding in the red pepper seed in the red pepper paste production in order to increase the use of the red pepper seed that is not used often as a food ingredient even though its nutrition value when producing red pepper paste was proven. Moisture content, pH, L-value, a-value, b-value, sugar contents, and salinity of instant Gochujang tended to increase as the amount of red pepper seed increased, while viscosity manifested the opposite trend. Sensory evaluations were conducted on instant Gochujang to assess differences in characteristics, which demonstrated that as the amount of red pepper seeds powder increased, the intensity of redness for the red pepper paste's external appearance, luster, spicy flavor and taste, and savory taste were evaluated strong. Meanwhile, fermentation value, which is red pepper paste's foremost weakness was evaluated as weak. Moreover, there was no difference in the size of the grain when compared to the contrast group consisting of regular red pepper paste. The results of preference test demonstrated that the RG3 with 30% of red pepper seed is optimal in terms of the external appearance, taste, texture and overall preference.

Pulsed MOCVD of Cu Seed Layer Using a (hfac)Cu(3,3-dimethyl-1-butene) Source and H2 Reactant (수소 환원기체와 (hfac)Cu(3,3-dimethyl-1-butene) 증착원을 이용한 Pulsed MOCVD로 Cu seed layer 증착 특성에 미치는 영향에 관한 연구)

  • Park Jaebum;Lee Jinhyung;Lee Jaegab
    • Korean Journal of Materials Research
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    • v.14 no.9
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    • pp.619-626
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    • 2004
  • Pulsed metalorganic chemical vapor deposition (MOCVD) of conformal copper seed layers, for the electrodeposition Cu films, has been achieved by an alternating supply of a Cu(I) source and $H_2$ reactant at the deposition temperatures from 50 to $100^{\circ}C$. The Cu thickness increased proportionally to the number of cycles, and the growth rate was in the range from 3.5 to $8.2{\AA}/cycle$, showing the ability to control the nano-scale thickness. As-deposited films show highly smooth surfaces even for films thicker than 100 nm. In addition about a $90\%$ step coverage was obtained inside trenches, with an aspect ratio greater than 30:1. $H_2$, introduced as a reactant gas, can play an active role in achieving highly conformal coating, with increased grain sizes.