• The Korean Journal of Ecology
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• 제18권3호
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• pp.353-366
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• 1995

To study the relationship between the germination response and geographical distribution, the seeds of Rumex acetocella were collected from nine sites (Taegwallyong, Alps lodge, Kans'ong, Sokch'o, Chumunjin, Suwon, Taechon, Sukgok, Mokpo) of different latitudes and altitudes. The populations of Rumex acetocella were subdivided into four groups based on the characteristics of the seed germination. The first group of Rumex acetocella (Taegwallyong, Alps lodge) germinated rapidly in early spring, the second group (Suwon, Teachon) in late summer through early autumn, and the third group(Sukgok, Mokpo) slowly in late autuman with various conditions. The thermal time values needed for the germination rate of 10% to 60% for these three groups indicated that the germination rate was low at the lower latitude, while it was increased as the latitude elevated. The population of the fouth group (Kansong, Sokcho, Chumunjin) showed the narrow optimum temperature range of germination response and the highest thermal time value to germinate 10% to 30% of seeds. The seeds in the high latitude region with relatively mild winter germinated synchronously in early autumn. These different germination responses of Rumex acetocella to geographic variations were thought to be an ecological strategy to survive in different environmental conditions.

• Journal of Ecology and Environment
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• 제35권2호
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• pp.73-78
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• 2012

$Calotropis$ $procera$ L. is a perennial shrub distributed in saline areas of deserts of South Asia. Salt stress is a very challenging subject in arid and semi-arid areas. Germination stage is very sensitive and many plants do not germinate in saline soil. The objective of this study was identifying the salinity effect on seed germination of $Calotropis$ $procera$ L. The experimental design was a complete randomized block design with NaCl and $CaCl_2$ at five levels of isobar concentrations: 0.0, -0.01, -0.05, -0.1, and -0.15 MPa. Osmotic potential had significant effects ($P$ < 0.01) on germination percentage, germination rate, shoot length, root length, and seedling dry weight. All seedling characteristics decreased with decrease in osmotic potential. Shoot length and root length decreased more than the seedling characteristics. Germination was completely inhibited in -0.1 Mpa. Priming with NaCl and $CaCl_2$ (-0.1 MPa) for four days had significant effects ($P$ < 0.01) on the germination percentages. Priming improved the seedling characteristics in all samples, especially in -0.05 Mpa, but a decrease with decrease in osmotic potential.

• Mycobiology
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• 제34권2호
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• pp.79-82
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• 2006

The germination rate and longevity of seeds of Gastrodia elata Blume have been observed for 48 weeks using Mycena osmundicola strain H-21, one of fungi stimulating seed germination. Storage condition of post-harvest seeds was observed in the different temperature ranges of $-30^{\circ}C,\;-5^{\circ}C,\;5^{\circ}C\;and\;30^{\circ}C$ for 48 weeks. After storage period of 48 weeks, the germination rate of G elata was 65.7% at $5^{\circ}C$ and 71.6% at $-5^{\circ}C$, respectively. Although the germination rate of G. elata was 77.3% for 11 weeks at $25^{\circ}C$, the germination rate had been decreased gradually to 49.3% at 13 weeks, 0.3% at 23 weeks and then 0% at 25 weeks. The germination rate was reached to the level of 10% for 2 weeks at $-30^{\circ}C$ and then decreased to 0%.

• 한국작물학회지
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• 제58권1호
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• pp.67-70
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• 2013

This research study was to develop methods for inhibiting the germination of cotton seeds. Germination rates after heating treatment at $80^{\circ}C$ with 10, 20, 30, 40 min. were 9.3, 9.3, 5.3 and 1.3 percentage, respectively. Heat treatment over $85^{\circ}C$ with 10 min. caused no germination of cotton seeds. Germination was significantly reduced with gamma rays treatment more than 10,000 gy, while the treatment within 2,000-8,000 gy induced less effects. Microwave processing for 30 seconds and 60 seconds inhibit the germination of cotton seed under 8 and 0%, respectively. As a physical treatment, rollmill milling with 1.5, 2, 2.5 mm gap inhibited any germination of cotton seeds. The optimum gap of roll-mill for processing physical cracking was below 51% of the thickness of seed.

• 한국작물학회지
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• 제44권3호
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• pp.263-266
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• 1999

Tobacco (Nicotiana tabacum L. ‘KF109’) seeds were primed in polyethylene glycol 6000 (PEG 6000) solutions to determine a) what osmotic potential of the solution would be optimal for priming, i.e., critical potential level for preventing germination, and b) what temperature and duration would be the most effective in priming. The germination was completely prevented below -0.8 MPa of PEG 6000, that indicates a optimum water potential for seed priming. Seeds were primed for 0, 1, 2, 3, 5, 10 and 15 days at 15, 20 and $25^{\circ}C$, respectively, under the-0.8 MPa PEG 6000 solution to find out the most effective temperature and duration for priming. The effectiveness of priming, particularly in germination speed, was observed more distinctly when the primed seeds were germinated at 15$^{\circ}C$ than 2 5$^{\circ}C$. The greatest reduction of the time to 50% germination (T/sob 50/) was when the seeds were primed at $25^{\circ}C$. The reduction rate of the $T_{50}$ was rapid when primed from 1 day to 8 days and then slowed down in the seeds primed for longer than 8 days. The time from 10 to 90% germination ( $T_{10-90}$ increased in the primed seeds for longer than 8 days which showed the reversed effect of synchronous germination. However, $T_{50}$ was reduced continuously in the seeds even primed over 8 days. Thus, the optimum condition for tobacco seeds priming with PEG 6000 solution was -0.8 MPa in osmotic potential of the solution at $25^{\circ}C$ for 8 days.ays.

• 원예과학기술지
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• 제19권1호
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• pp.78-80
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• 2001

금낭화의 실생번식방법을 확립하기 위하여 $GA_3(100mg{\cdot}L^{-1})$와 냉습저장($3^{\circ}C$) 그리고 배병의 제거가 금낭화의 발아에 미치는 영향을 조사하였다. $GA_3$를 처리하지 않은 종자는 90일간의 냉습저장에도 발아를 하지 않았다. 그러나 $GA_3$처리는 냉습저장에 관계없이 금낭화의 발아를 촉진하였다. 배병을 제거한 경우 $GA_3$처리에 의한 발아촉진효과가 감소하는 경향을 나타내었다. $GA_3$처리 후 냉습저장에 의해서 또한 냉습저장 기간이 길어질수록 발아일수는 단축되었으며 발아속도는 촉진되었다. 이상의 결과로부터 $GA_3$처리는 금낭화의 발아촉진에 효과적이며 냉습저장처리는 $GA_3$의 효과에 상승적인 작용을 하는 것을 알 수 있었다.

• 한국자원식물학회지
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• 제13권3호
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• pp.202-207
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• 2000

자생식물 16종의 종자형질 특성 및 저온습윤 및 변온 처리에 의한 발아양상을 조사하기 위하여 본실험은 수행되었다. 종에 따라 종자의 길이, 넓이, 폭은 각각 1.1∼8.9, 0.7∼7.5, 0.4∼l.7mm의 범위였으며 천립중은 0.1∼8.8g의 범위였다. 무처리에서 발아율이 가장 높은 종에 있어 냉이와 익모초는 암조건에서 각각 16, 36%이었으며 장구채와 각시취는 명조건에서 각각 69, 81%이었다. 참취, 고려엉겅퀴, 곰취, 질경이, 청옥취는 저온습윤처리 명조건에서 각각 84, 29, 57, 78, 95%로 물엉겅퀴는 암조건에서 30%로 발아율이 가장 높았다. 변온처리에서 발아율이 높은 종에 있어 두릅나무와 벌개미취는 명조건에서 각각 2,57%로, 수리취는 암조건에서 52%로 가장 높았다.

• 한국작물학회지
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• 제48권4호
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• pp.334-338
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• 2003

The objective of this study was to demonstrate whether or not the deleterious effects of accelerated aging on seed vigour and viability are alleviated by interaction with gamma irradiation. Seeds of soybean (Glycine max L.) were artificially aged and subsequently irradiated with 4 and 8 Gy of gamma irradiation. Germination rate was negatively affected by accelerated aging and positively by gamma irradiation, with a positive interaction of a 3day-seed aging treatment occurring with 4 Gy, possibly suggesting that 4 Gy of gamma irradiation partially offset the adverse effects of seed aging on germination. However, 5-day aged seeds did not gain any benefits from the gamma irradiation. Electrolyte leakage from the seeds increased with the duration in days aged. Irradiation, however, did not impose any effects on the leakage. Respiration rate of the seed with hypocotyl and primary root was significantly low for the aged seeds, but not for the seeds with both irradiation and aging treatments. Accelerated aging decreased the dry weight of the hypocotyl and primary root of the seeds without any measurable effects of irradiation. $\alpha$-Amylase activity decreased with seed aging and positively responded to gamma irradiation. The data is discussed with regard to the possible roles of gamma irradiation for improving the seed vigour and viability of aged seeds.

• Journal of Ginseng Research
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• 제25권4호
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• pp.167-170
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• 2001

개갑 인삼종자의 생리적 휴면타파를 위한 적정 저온감응기간을 조사하였다. 채종하여 3개월간 모래로 층적저장하여 개갑이 완료된 종자의 배는 배유의 1/2정도의 크기를 보였다. 인삼종자는 채종 당시의 미숙한 배가 형태적으로 거의 완전한 발달을 보이기까지는 6개월 정도가 소요되었으며, 배의 신장의 실외보다는 실내에 저장하였던 종자가 더 빨리 자랐다. 배 신장이 완료된 종자라 할지라도 60일 이하의 저온처리된 종자는 발아가 되지 않았으며, 발아에 필요한 저온감응기간은 실외의 자연상태에서는 75일 정도이며, 냉장온도(4$^{\circ}C$)에서는 90일 정도가 적정하였다. 그러나 적정의 저온감응을 받은 종자라 할지라도 상온데 지속적으로 방치하게 되면 그 효과는 현저히 감소되었다.

• 한국자원식물학회지
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• 제27권5호
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• pp.534-539
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• 2014

본 연구는 식물구계학적 특정종 III 등급이며, 국외반출 시 승인이 필요한 보호종인 등수국의 종자 번식법을 개발하기 위해 수행되었다. 종자의 크기는 $1.36{\times}0.84mm$이며, 1,000립 중은 $2.3{\pm}0.02mg$이었다. 종자는 타원형으로 표면에 주름이 있었으며, 황색의 미립종자였다. 침지처리에 의해 종자의 함수율은 48시간 만에 3.9%에서 31.9%로 급속히 증가하여 최대 함수율을 보였다. 온도 및 광조건에 따른 발아율은 $25^{\circ}C$, 명조건에서 가장 높았으며(78.0%), $GA_3$ 용액에 48시간 처리함으로써 발아율, 발아세 및 $T_{50}$이 매우 향상되었다. 특히 $GA_3$ 처리구는 $100{\sim}500mg{\cdot}L^{-1}$의 농도에서 발아율이 90.0% 이상으로 매우 우수였으며 발아세와 $T_{50}$을 무처리에 비해 향상시킬 수 있었으므로, $GA_3$는 등수국 종자의 발아력을 효과적으로 향상시킬 수 있는 적절한 화학적 처리방법으로 판단되었다.