• Title/Summary/Keyword: Seed factor

Search Result 298, Processing Time 0.03 seconds

QTL mapping of low-temperature germinability and identification of qLTG1 candidate genes in rice

  • Kim, Sun Ha;Shim, Kyu-Chan;Lee, Hyun-Sook;Le, Anh Quynh;Ahn, Sang-Nag
    • Proceedings of the Korean Society of Crop Science Conference
    • /
    • 2017.06a
    • /
    • pp.116-116
    • /
    • 2017
  • Low-temperature is one of the environmental stress factors that affect plant growth and development and consequently limit crop productivity. The control of seed germination under low-temperature is organized by many genes which are called quantitative trait loci (QTLs). High germination rate for low-temperature is an important factor of growing rice. Previously, we identified a major QTL controlling low-temperature germinability in rice using 96 introgression lines (ILs) derived from a cross between Oryza rufipogon (Rufi) and the Korean japonica cultivar, 'Hwaseongbyeo (HS)'. A $BC_3F_7$ line (TR5) showed better low-temperature germinability than its recurrent parent. TR5 was crossed with HS to develop a segregating F2:3 populations for the target QTL. Six SSR markers polymorphic between HS and Rufi were used to screen and fine map the qLTG1. The qLTG1 on chromosome 1, which accounted for 55.5% of the total phenotypic variation, confirmed that Rufi allele enhanced the low-temperature germinability. Intervals between markers CRM16 and CRM15, four candidate genes were identified. The identified candidate genes, which are encoded by a protein of unknown function, showed their direct involvement on seed germination at low-temperature. To identify genes targeted by qLTG1, we investigated the expression profiles of these candidate genes and germination behavior of qLTG1 under different stress conditions and compared to HS, Rufi, and TR5 at $13{\pm}2^{\circ}C$ for 3 days after incubation. Furthermore, transgenic rice plants will also be developed to conduct a detailed investigation on low-temperature germinability. Hence, the QTL for low-temperature germinability would be useful in rice breeding programs especially in the development of lines possessing low-temperature germinability.

  • PDF

Comparison of Morphological Characters and Stable Isotopes of Seeds between Wild Simulated Ginseng and Cultivated Field Ginseng (국내 산양삼과 인삼 종자의 형태적 형질과 안정성 동위원소 비교)

  • Choi, Myoung Sub;Kim, Sunhee;Park, Chan Ryul;Kim, Namyoung;Shin, Jinsub
    • Journal of Korean Society of Forest Science
    • /
    • v.97 no.4
    • /
    • pp.357-362
    • /
    • 2008
  • We compared the morphological characters and stable isotopes of seeds of wild simulated ginseng with those of cultivated field ginseng. Seeds were collected from ten areas for wild simulated ginseng and two areas for cultivated field ginseng. The length, width, thickness and number of seeds per gram were measured and the ratio of stable isotopes of carbon and nitrogen was analysed as well. There was a overlapping variation of morphological characters and the ratio of stable isotope of the wild simulated ginseng among study areas. In Geumsan area, compared with the seed of cultivated field ginseng, the seed of wild simulated ginseng was significantly small and light, and the ${\delta}^{15}N$(‰) of wild simulated ginseng was lower than that of cultivated field ginseng. However, it is somewhat impossible to compare the regional variation of the unique value of ${\delta}^{15}N$(‰) among study areas of wild simulated ginseng. We can suggest the value of ${\delta}^{15}N$(‰) can be used as detection factor for cultivation regime like chemical fertilization and organic farming.

Studies on Wood Quality and Growth of Alnus glutinosa (L.) Gaertn. in Korea - Physical Properties - (글루티노사오리나무의 생장과 재질에 관한 연구 - 물리적 성질 -)

  • Han, Ju-Hwan;Jeong, Jae-Hun;Lee, Gooi-Yong;Kim, Byung-Ro
    • Journal of the Korean Wood Science and Technology
    • /
    • v.43 no.1
    • /
    • pp.1-8
    • /
    • 2015
  • Relationship between annual ring width and physical properties of 24-year old alder woods (Alnus glutinosa L.) that had different seed origins from four provenances such as Bulgary, Italy, United Kindom, and Yugoslavia was investigated. No difference among different origins of seeds was found for the moisture content, density, and shrinkage or tangential radial (T/R) ratio. These properties had also no relationship with the growth rate. As important parameters among wood quality indices, no difference in the density or shrinkage on wood that has different growth rates was detected among alder woods from four different origins of seeds. Therefore, it is considered that higher growth rate might be a key factor for selecting the seed origin of the alder tree.

Protective Effects of Combination of Carthamus tinctorius L. Seed and Taraxacum coreanum on Scopolamine-induced Memory Impairment in Mice (홍화씨와 흰민들레 복합물의 Scopolamine 유도 기억력 손상에 대한 보호 효과)

  • Kim, Ji Hyun;He, Mei Tong;Kim, Min Jo;Park, Chan Hum;Lee, Jae Yang;Shin, Yu Su;Cho, Eun Ju
    • Korean Journal of Medicinal Crop Science
    • /
    • v.28 no.2
    • /
    • pp.85-94
    • /
    • 2020
  • Background: Alzheimer's disease (AD) is caused by various factors, such as cholinergic dysfunction, regulation of neurotrophic factor expression, and accumulation of amyloid-beta. We investigated whether or not a combination of Carthamus tinctorius L. seed and Taraxacum coreanum (CT) has a protective effect on scopolamine-induced memory impairment in a mouse model. Methods and Results: Mice were orally pretreated with CT (50, 100 and 200 mg/kg/day) for 14 days, and scopolamine (1 mg/kg/day) was injected intraperitoneally before subjecting them to behavior tests. CT-administered mice showed better novel object recognition and working memory ability than scopolamine-treated control mice. In T-maze and Morris water maze tests, CT (100 and 200 mg/kg/day) significantly increased space perceptive ability and occupancy to the target quadrant, respectively. In addition, 100 and 200 mg/kg/day of CT attenuated cholinergic dysfunction through inhibition of butyryl cholinesterase in brain tissue. Furthermore, CT-administered mice showed higher cyclic adenosine monophosphate-response element-binding protein (CREB) levels and lower amyloid precursor protein (APP) levels compared to scopolamine-treated control mice. Conclusions: CT improved scopolamine-induced memory impairment through inhibition of cholinergic dysfunction, up-regulation of CREB, and down-regulation of APP. Therefore, CT could be a useful therapeutic agent for AD with protective effects on cognitive impairment.

Management of Earthworm Casts using Pesticides and Tea Tree (Camellia oliefera) Extracts in Turfgrass of Golf Courses (골프장에서 차나무류 추출물과 화학 방제제를 이용한 지렁이 분변토 발생 관리)

  • Shin, Chong Chang;Kim, Jong Kyung;Choi, Sung Hwan;Jeon, Byungduk;Lee, Dong Woon
    • Weed & Turfgrass Science
    • /
    • v.5 no.1
    • /
    • pp.35-41
    • /
    • 2016
  • Earthworm cast is a harmful factor which reduces the uniformity of management of turfgrass and play quality in golf courses and playground. This research was conducted to carry out the efficacy of some pesticide (bifenthrin GR + clothianidin GR, ethoprophos GR, imidacloprid SC, picoxystrobin WG, thiophanate-methyl WP) and tee tree (Camellia oliefera) extracts against reducing earthworm cast in golf courses. All tested materials were acted reducing earthworm cast, thiophanate-methyl among them showed the highest effect reducing earthworm casts in golf courses. Tea tree extracts was occurred similar efficacy among tested chemical pesticides. However, pellet type byproduct of tea seed extract and liquid formulation of tea tree extract were better than granule formulation of them. Byproduct of tea seed and tea tree extract can be used for alternative biological management material for reducing earthworm cast in heavily casting occurrence area.

Analysis of Early Revegetation Effect in Rock Slopes using Vegetation-Plant (식생플랜트를 이용한 암반비탈면의 조기녹화 효과분석)

  • Ma, Ho-Seop;Kang, Won-Seok;Park, Jin-Won
    • Journal of the Korean Society of Environmental Restoration Technology
    • /
    • v.13 no.5
    • /
    • pp.81-89
    • /
    • 2010
  • This study was conducted to evaluate the effects of early revegetation by analyzing the characteristics of germination and growth of Chrysanthemum zawadskii using vegetation-plant in rock slopes. After making up a growing basis of approximately 20-cm depth and 10-cm diameter by using a boring machine, the surface of rock slopes was planted with vegetation-plant. The number of germinating populations by soil media was 41 in H.s, 4 in T.s, 3 in M.s, and 0 in M.g.s. The germination rate (%) by soil media was 20.0% in H.s, 3.3% in T.s, 2.5% in M.s and 0% in M.g.s. In monthly changes of growth rate, the aspect was northwest direction, the soil media was H.s, and the treatment was microorganism plot. The main factors affecting survivorship and growth of population were soil media and treatment plot. The interaction between each factor had a good effects in bearing x treatment plot, soil media x treatment plot. but, it is recommended that the mulching of vegetation plant is highly needed to help the germination of seed and growth of vegetation because of loss of seed and soil media occurred due to rainfall. Therefore, The result suggests that the revegetation technique using boring in rock slope was very efficient in respect of the early revegetation and the landscape.

Application of a Selective Emitter Structure for Ni/Cu Plating Metallization Crystalline Silicon Solar Cells (Selective Emitter 구조를 적용한 Ni/Cu Plating 전극 결정질 실리콘 태양전지)

  • Kim, Min-Jeong;Lee, Jae-Doo;Lee, Soo-Hong
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.23 no.7
    • /
    • pp.575-579
    • /
    • 2010
  • The technologies of Ni/Cu plating contact is attributed to the reduced series resistance caused by a better contact conductivity of Ni with Si and the subsequent electroplating of Cu on Ni. The ability to pattern narrower grid lines for reduced light shading was combined with the lower resistance of a metal silicide contact and an improved conductivity of the plated deposit. This improves the FF (fill factor) as the series resistance is reduced. This is very much requried in the case of low concentrator solar cells in which the series resistance is one of the important and dominant parameter that affect the cell performance. A Selective emitter structure with highly dopeds regions underneath the metal contacts, is widely known to be one of the most promising high-efficiency solution in solar cell processing In this paper the formation of a selective emitter, and the nickel silicide seed layer at the front side metallization of silicon cells is considered. After generating the nickel seed layer the contacts were thickened by Cu LIP (light induced plating) and by the formation of a plated Ni/Cu two step metallization on front contacts. In fabricating a Ni/Cu plating metallization cell with a selective emitter structure it has been shown that the cell efficiency can be increased by at least 0.2%.

Inheritance of Cgy1 gene and Ti gene in Mature Soybean Seed

  • Sung, Mi-Kyung;Han, Eun-Hui;Kim, Kyung-Roc;Park, Jung-Soo;Hwang, Kyo-Jin;Nam, Jin-Woo;Chung, Jong-Il
    • Korean Journal of Breeding Science
    • /
    • v.42 no.1
    • /
    • pp.35-39
    • /
    • 2010
  • Soybean proteins are widely used for human and animal feed in the world. ${\beta}$-conglycinin protein exhibits poor nutritional and food processing properties and Kunitz trypsin inhibitor (KTI) protein is a main anti-nutritional factor in soybean seed. The objective of this research was to identify the inheritance of $cgy_1$ gene and ti gene for the improvement of soybean cultivar with no KTI proteins and low amount of ${\beta}$-conglycinin. $F_2$ population was made by crossing between "Gaechuck2ho" (${\alpha}^{\prime}$-subunit present $Cgy_1Cgy_1$, KTI protein absent titi) and PI506876 (${\alpha}^{\prime}$-subunit absent $cgy_1cgy_1$, KTI protein present TiTi) parent. A total of 434 $F_2$ seeds were obtained and analyzed for the segregation of ${\alpha}^{\prime}$-subunit protein and KTI protein using SDS-PAGE. The segregation ratio of 3 : 1 for $Cgy_1$ locus (310 $Cgy_1$_ : 124 $cgy_1cgy_1$) and Ti locus (339 Ti_ : 95 titi) were observed. Segregation ratios of 9 : 3 : 3 : 1 (241 $Cgy_1$_Ti_: 69 $Cgy_1$_titi: 98 $cgy_1cgy_1$Ti_: 26 $cgy_1cgy_1titi$) between $Cgy_1$ gene and Ti gene in $F_2$ seeds were also observed (${\chi}^2= 5.367$, P = 0.10 - 0.20). This data showed that $Cgy_1$ gene was inherited independently with the Ti gene in soybean. These results will be useful in breeding program for selecting the line that does not exhibit or lacks both ${\alpha}^{\prime}$-subunit protein and KTI protein in soybean.

Evaluation of Anti-thrombosis Activities of Different Parts of Cannabis sativa L. (대마 부위별 추출물의 항혈전 활성)

  • Kang, Deok-Gyeong;Kim, Young-Min;Sohn, Ho-Yong
    • Journal of Life Science
    • /
    • v.31 no.6
    • /
    • pp.581-586
    • /
    • 2021
  • The high fatality of COVID-19 (Corona Virus Disease 2019) is closely related to acute pneumonia and severe blood clot formation in brain, heart, kidney and lung. The use of low-molecular weight heparins has been shown to reduce the risk of thrombosis and reduce fatality rates among COVID-19 patients. In this study, we investigated the antithrombotic activity of different parts of C. sativa extracts to determine its potential in preventing blood clots in patients with thromboprophylaxis. The extracts of leaf, stem, root, immature flower and seed of C. sativa showed strong inhibitory activities on blood clot formation. In particular, the flower extract showed the strongest inhibitions against blood coagulation factors and thrombin. Strong hemolysis activities were observed in flower extract and seed extract, suggested that removal of the hemolysis active compounds in th extracts is necessary. This is a first report of anti-thrombosis activities of different parts of C. sativa extracts, and our results suggest that C. sativa extract has potential has a valuable bioresource for high-value products.

Study of the Variation of Optical Amplification Characteristics with Incident Beam Size and Temperature of a Cesium-vapor-based Optical Amplifier (세슘 원자 증기 기반 광 증폭기의 온도와 빔 크기에 따른 광 증폭 특성 연구)

  • Ryu, Siheon;Jeong, Yujae;Yeom, Dong-Il
    • Korean Journal of Optics and Photonics
    • /
    • v.32 no.6
    • /
    • pp.306-313
    • /
    • 2021
  • We study the amplification properties of an optical amplifier based on a cesium-vapor cell. An optical amplification system including cesium vapor mixed with a buffer gas is built, and its amplification feature is investigated as a function of the size of the incident beam and the temperature of the cesium-vapor cell. We observe that the optical amplification properties, such as amplification factor and extraction efficiency, change significantly depending on the temperature and beam diameter of the pump and seed light. A maximum extraction efficiency of 56% is obtained when the temperature of the cesium cell is 90 ℃, with a 200-㎛ diameter of the pump (500 mW) and seed light (10 mW). The numerical simulation of the amplification properties agrees reasonably with the results obtained from the experiment.