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http://dx.doi.org/10.7783/KJMCS.2020.28.2.85

Protective Effects of Combination of Carthamus tinctorius L. Seed and Taraxacum coreanum on Scopolamine-induced Memory Impairment in Mice  

Kim, Ji Hyun (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University)
He, Mei Tong (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University)
Kim, Min Jo (Institute of Natural Medicine, Toyama Medical and Pharmaceutical University)
Park, Chan Hum (Farmdew Inc.)
Lee, Jae Yang (Insan Life Science Research Institute, Insan Inc.)
Shin, Yu Su (Department of Herbal Crop Research, NIHHS, RDA)
Cho, Eun Ju (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.28, no.2, 2020 , pp. 85-94 More about this Journal
Abstract
Background: Alzheimer's disease (AD) is caused by various factors, such as cholinergic dysfunction, regulation of neurotrophic factor expression, and accumulation of amyloid-beta. We investigated whether or not a combination of Carthamus tinctorius L. seed and Taraxacum coreanum (CT) has a protective effect on scopolamine-induced memory impairment in a mouse model. Methods and Results: Mice were orally pretreated with CT (50, 100 and 200 mg/kg/day) for 14 days, and scopolamine (1 mg/kg/day) was injected intraperitoneally before subjecting them to behavior tests. CT-administered mice showed better novel object recognition and working memory ability than scopolamine-treated control mice. In T-maze and Morris water maze tests, CT (100 and 200 mg/kg/day) significantly increased space perceptive ability and occupancy to the target quadrant, respectively. In addition, 100 and 200 mg/kg/day of CT attenuated cholinergic dysfunction through inhibition of butyryl cholinesterase in brain tissue. Furthermore, CT-administered mice showed higher cyclic adenosine monophosphate-response element-binding protein (CREB) levels and lower amyloid precursor protein (APP) levels compared to scopolamine-treated control mice. Conclusions: CT improved scopolamine-induced memory impairment through inhibition of cholinergic dysfunction, up-regulation of CREB, and down-regulation of APP. Therefore, CT could be a useful therapeutic agent for AD with protective effects on cognitive impairment.
Keywords
Carthamus tinctorius L.; Taraxacum coreanum; Cognition; Memory; Scopolamine;
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