• Korean Journal of Food Science and Technology
• /
• v.52 no.1
• /
• pp.40-45
• /
• 2020

Over 500 genetically modified organisms (GMOs) have been developed since 1996, of which nearly 44% have glufosinate herbicide-tolerant traits. Identification of specific markers that can be used to identify herbicide-tolerant traits is challenging as the DNA sequences of the gene(s) of a trait are highly variable depending on the origin of the gene(s), plant species, and developers. To develop specific PCR marker(s) for the detection of the glufosinate-tolerance trait, DNA sequences of several pat or bar genes were compared and a diverse combination of PCR primer sets were examined using certified reference materials or transgenic plants. Based on both the qualitative and quantitative PCR tests, a primer set specific for pat and non-specific for bar was developed. Additionally, a set of markers that can detect both pat and bar was developed, and the quantitative PCR data indicated that the primer pairs were sensitive enough to detect 0.1% of the mixed seed content rate.

• Korean Journal of Soil Science and Fertilizer
• /
• v.4 no.2
• /
• pp.143-147
• /
• 1971

Field experiments were carried out to determine the effect of B on rape with application of lime and compost in sandy upland soil and in clayey paddy soil. The plant samples from each treatment at flowering stage were analyzed to interpret the yield response. The effect of B was very significant in both soils, but more marked in sandy upland soil. The application of 20kg/ha borax appears to increase upto the possible level that can be achieved by B application. Application of 40kg/ha borax did not give any further additional response. In acidic paddy soil (pH 5.4), the effect of B was fully exhibited only with liming. Liming neither intensified the B deficiency nor reduced the B content in the plant tissue, but rather promoted the growth of rape plant. There was highly significant negative correlation between Ca/B ratio and seed yield ($r=-0.74^{**}$), but it was very difficult to draw a line between normal and deficient Ca/B ratio. It is very interesting that boron application significantly increased the Ca uptake in no lime treatment. Application of compost was generally inffective and did not affect the B supply to the plant. However, it appears to increase K uptake slightly and give a little favorable effect on growth of plant.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.5
• /
• pp.671-676
• /
• 2005

A total of 50 individual catfish, the Tra (Pangasius hypophthalmus) cultivated in either floating cages (Tra-c) or in ponds (Tra-p) and the Basa (Pangasius bocourti) raised in three floating cages, were collected in two of the Mekong Delta provinces. The caudal fin of each individual fish was used for protein electrophoresis employing the SDS-PAGE method. The one fillet sides were used as a representative sample to determine the dry matter (DM), crude protein (CP), ether extract (EE) and amino acids (AAs). The catfish oil was extracted from the belly fats, and the fatty acid (FA) composition was analyzed. There were 21 bands of the Tra and the Basa. Protein bands of the two varieties were 28.6-33.3% polymorphic, while polymorphic individuals of the Tra ranged from 80.0 to 100.0%, and the Basa was 90.0% polymorphic. The phenotypic diversity (Ho) of the Tra ranged from 1.71 to 1.80, while the Basa ranged as high as 2.14%. Diversity values (H$_{EP}$) for genetic diversity markers were equal in the Tra and the Basa. The sum of the effective number of alleles (SENA) of both varieties ranged from 3.40 to 3.83 for the Basa and the Tra, respectively. The lower values of Ho and SENA, as compared with those of the fresh water prawn (Macrobrachium equidens) in the area, would suggest that the species with the low values will become extinct due to inbreeding; the gene pools of each observed population were below a suitable threshold. Many of the differences in the nutritional values of the Tra-c, the Tra-p and the Basa were measured; their nutrient values were comparable to fishmeal or fish oil. Most of the DM, CP, and EE were higher in the Tra, especially in the Tra-c. The essential AA content, especially that of lysine, was highest in the Tra-c, next highest in the Tra-p, and lowest in the Basa. Therefore, the amino acid patterns were closer to the ideal patterns in the same sequences. In contrast, the essential FAs were concentrated in the Basa fish oil. It was found that suitable selection of parents for seed production is required to avoid inbreeding. Catfish may be valuable sources of nutrition for both humans and animals, and the differences in their nutritional values by variety and/or management must be taken into account.

• Journal of Mushroom
• /
• v.18 no.3
• /
• pp.268-273
• /
• 2020

This study aimed to assess the feasibility of composting spent mushroom substrate (SMS) materials of Lentinula edodes (Le), Hericium erinaceus (He), and Pleurotus ostreatus (Po). The different SMSs were composted for 7 to 10 days at high temperatures over 50℃; the composting procedure was completed in 30 days. A maturity test was conducted using the radish seed germination index and CoMMe-100. The composted SMS (CSMS) from Le and He showed gemination indices of 130% and 81%, respectively, that satisfied the criteria of maturity standard (gemination index over 70%) and the CoMMe-100 analysis. The physicochemical changes of CSMSs included an increase in the pH range from 4-5 to 6-7, slight reduction in the EC to 1-1.4 dS/m, and an organic content of 36.9% in LeCSMS. In LeCSMS, the contents of N, P, and K were 1.2%, 2.3%, and 0.77%, respectively, and heavy metals were detected below the standard value in all CSMSs; the Ca and Mg contents in the CSMSs were increased from 30% to 60% when compared to those in the SMSs. The C/N ratio (from 26-33) in LeSMS and HeSMS decreased to 15.3-15.9 in CSMSs. The growth effect of LeCSMS treatment on pepper seedlings was 60% higher than that in the control groups, one of which was treated with commercial organic compost; the former showed a superior growth effect on the leaf width, leaf length, and leaf number compared to other control groups. In conclusion, LeCSMS and HeCSMS could be utilized as compost resources capable of efficient soil amendment and plant growth promotion.

• Proceedings of the Korea Society of Poultry Science Conference
• /
• 2001.11a
• /
• pp.24-39
• /
• 2001

These experiments were conducted to evaluate the nutritional and feeding value of red pepper seed oil meal (RPSOM) as local vegetable protein ingredients for poultry feeding. In the first experiment, nutritional values of RPSOM were evaluated by analyzing chemical compositions and determining true metabolizable energy (TME), nitrogen corrected TME (TMEn) and true available amino acid (TAAA) contents. According to the chemical analysis, RPSOM contained 22.50％ of crude protein, 4.75％ of ether extract, 27.70％ of crude fiber, 0.34 mg／g of capsaicin and 49.97 ppm of xanthophylls. The values of TME and TMEn determined by force-feeding 16 roosters (ISA-Brown) were 1.73 kcal／g and 1.63 kcal／g on dry matter basis, respectively. The average TAAA value of 16 amino acids measured by the force－feeding technique was 81.70％. These values were used for formulating experimental diets containing various levels of RPSOM for broiler chicks and laying hens. Two feeding trials were made to investigate the effects of dietary incorporation of RPSOM into chicken feed performances of broiler chicks and laying hens. In the broiler feeding (Exp. 2), a total of two hundred twenty-five, 4 wk old male broiler chicks (Ross) were randomly divided into 9 groups of 25 birds each and assigned to three experimental diets containing 0, 5 and 10% RPSOM. The birds were fed ad libitum the diets for 3 wk and feed intake, weight gain and feed conversion rate were determined. At the end of the feeding, the blood levels of glutamate oxaloacetate transaminase (GOT), gamma glutamyl transpeptidase (GGT), blood urea nitrogen (BUN) and cholesterol, and the body and fatty acid compositions of leg muscle were measured. No significant differences were observed in weight gain, feed intake, feed conversion rate, body composition, serum levels of GOT, GGT and BUN among the treatments. However, blood cholesterol level was lower (P＜0.05) in 10% RPSOM diet group than those in the other. The dietary RPSOM at 5 and 10% levels increased the content of linoleic acid (P＜0.05) in leg muscle compared to that of control group. The results indicate that RPSOM can be used for broiler feed up to 10% without any significant negative effects on broiler performance. In the layer feeding (Exp. 3), the effects of dietary RPSOM on the performances of laying hen were investigated by feeding ninety 45 wk old laying hens (ISA-Brown) with experimental diets containing 0, 5 and 10% RPSOM for 4 wk (30 birds per treatment). Measurements were made on egg production rate, egg weight, feed intake, Haugh unit, egg shell strength which was higher (P＜0.05) in layers fed 10% RPSOM diet compared to those fed 0 and 5% RPSOM diets. Thus, it can be concluded that RPSOM can be included into laying hen feed up to 10% without any harmful effects.

• Korean Journal of Weed Science
• /
• v.8 no.3
• /
• pp.283-290
• /
• 1988

This study was conducted to clarify the identification of phytotoxic compounds from 5 upland weeds which had been collected at their maturity age in the field of Kyungpook province, Korea. 5 weed species including Stellaria aquatica strongly inhibited the lettuce seed germination. These 5 species were Amaranthus lividus, Stellaria aquatica, Achyranthes japonica, Polygonum perfoliata and Galinsoga ciliata. the degree of inhibitory effects according to various solvent extracts such as ethanol, aceton, chloroform and ether was different as species. In total phenol and diphenol content G. ciliata had largest amount of total phenol with 2.75mg/g, P. perfoliata 1.8mg/g, S. aquatica 1.7mg/g, A. lividus 1.28mg/g, A. japonica 1.27mg/g respectively. A. lividus had much amount of p-cresol, hydroquinone, ferulic, caffeic, p-cl-benzoic and p-coumaric in order. S. aquatica, A. japonica, P. perfoliata and G. ciliata had a large quantity of ferulic, caffeic, 7-OH-coumarin and protocatechuic acids in common. The amount of fatty acids was existed 8.49mg/g in S. aquatica, 7.14mg/g in A. japonica, 7.10mg/g in G. ciliata, 5.49mg/g in P. perfoliata and 4.2mg/g in A. lividus respectively. Most of 4 weeds except G. ciliata had much oxalic acid to the extent of 14-22mg/g. however it appeared that G. ciliata had much quantity of feruic and citric acids.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.39 no.5
• /
• pp.465-472
• /
• 1994

To improve the germination performance of rice seeds under suboptimal temperature, osmopriming with PEG-6000 was examined. Optimal PEG-6000 concentration to improve germination was 20% PEG-6000 solution, and rice cultivars used in this experiment were Sinunbongbyeo, Gancheokbyeo, Dongjinbyeo. The water content of seeds after soaking for 60 hours in the PEG solution is similar to that of seeds after soaking for 24 hours in the distilled water. Germination performance of the soaked seeds in the PEG solution was higher than that of the soaked seeds in the distilled water or the control, especially under suboptimal temperatures. Electrical conductivity of the soaked seeds in the PEG solution was lower than that of the soaked seeds in the distilled water or the control, and total dehydrogenase activity of the soaked seeds in the PEG solution was higher than that of the soaked seeds in the distilled water or the control. SDS-PAGE results of soluble protein from the embryos of seeds primed differently showed darker band in the seeds soaked in the PEG solution than the seeds soaked in the distilled water or the control at the 68 KD region. Also, band patterns of peroxidase and esterase of embryos soaked in the PEG solution were darked than that of embryos soaked in the distilled water or the control at the Rf 0.94 and Rf 0.87, respectively.

• Korean Journal of Plant Tissue Culture
• /
• v.26 no.3
• /
• pp.197-203
• /
• 1999

The albino plants of tobacco (Nicotiana tobacum L. cv. BY-4) were isolated from seed populations that were induced by heavy-ion ($^{14}N$) beam irradiation to proembryo and the in vitro growth and differentiation have been characterized. The in vitro cultured albino plants showed significant reduction of chlorophyll content and possessed larger number of stomata on both upper and lower epidermis than that of wild-type plants. Stem growth of the mutants remained dwarfed, however, the internode recovered its normal length after GA$_3$ treatment (10.0mg/L) on the MS medium containing sucrose under continuous light. When explants of leaf blades of albino plants were cultured, multiple shoots formed directly on MS medium containing 1.0mg/L of BAP or kinetin and a large number of calli were induced on the MS medium containing 1.0mg/L NAA or 1.0 mg/L 2,4-D. The albino calli regenerated multiple albino plantlets in the MS medium containing 0.1mg/L NAA + 1.0 mg/L BAP. No significant differences between the wild-type and albino plants were detected in the multiple shoot induction, callus formation from the explants and the plantlets regeneration from calli. In addition, albino plants have a similar organogenesis Pattern to that of the wild-type in the media with different combinations of NAA (0 to 5.0mg/L) and BAP (0 to 5.0mg/L) treatment. These results indicate that the albino mutant has the same normal regeneration ability as that of wild-type, although the mutant has lost functions in photosynthesis, such as pigmentation.

• Applied Biological Chemistry
• /
• v.24 no.1
• /
• pp.7-14
• /
• 1981

Among the Basidiomycetes, Pleurotus ostreatus 301 and Lentinus edodes 3-1 were chosen because of their good enzyme productivity and rapid mycelial growth in rice straw medium. Their cultural conditions adequate for the enzymes production and effects of various materials and inorganic salts added to thd rice straw media were investigated. L. edodes 3-1 was excellent in productivity of cellulase and xylanase, and P. ostreatus 301 in protease. The optimum conditions for enzyme production were at $30^{\circ}C$ in cellulase production and at $25^{\circ}C$ in xylanase and protease production, with 75% moisture content and 5.0-6.0 initial pH. The appropriate cultural periods, for enzyme production were 30 days and 35 days for P. ostreatus 301 and L. edodes 3-1, respectively. Among the various materials added, defatted soybean, defatted rape seed, or defatted sesame were all effective to enzyme production but reduced mycelial growth. Rice bran was also effective, particularly at 30% concentration. The addition of inorganic salts showed effective to enzyme production. Among inorganic salts, optimum concentration of $CaCO_3$ was 5%, and that of $CaSO_4$ was 2%.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.33 no.2
• /
• pp.169-173
• /
• 1988

In order to find out the optimum seeding date of flax. this study was investigated ecological characters, yield components and yield of five varieties(Wiera, Taijungsun ＃$^1$, Storment Goss, CI 1763, 99403-Fiber). As seeding date was delayed, the maturing date was delayed also and stem length was longest in March 10 seeding but it grew short in the late seeding. Oil content was increased most in March 10 seeding, that is, increased to 34% in Taijungsun ＃$^1$, 32% in storment Goss, 30% in Wiera and 26% in 99403-Fiber, and it was decreased in the late seeding. Stem yield and seed yield were heaviest in March 10 seeding, and it was light in the late seeding, Judging from the results reported above. in optimum seeding date of flax seemed to be before the March 10.