• Journal of Forest and Environmental Science
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• v.38 no.3
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• pp.141-151
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• 2022

Soil seed banks as natural storage of plant seeds play an important role in the maintenance and regeneration of watershed. Natural regeneration potential of the soil seed bank of Land use types (LUTs) in Ogun River watershed (ORW) was investigated. ORW was stratified using proportionate sampling technique into Guinea Savannah (GS), Rainforest (RF) and Swamp Forest (SF) Ecological Zones (EZs). Three LUTs: Natural Forest (NF), Disturbed Forest (DF) and Farmland (FL) were purposively selected in GS: GSNF, GSDF, GSFL; RF: RFNF, RFDF, RFFL and SF: SFNF, SFDF, SFFL, respectively. Systematic line transects was used in the laying of the sample plots. Sample plots of 25 m×25 m were established in alternate positions. Ten 1 m×1 m quadrats were randomly laid for soil core sampling from previously randomly selected ten plots. The core samples (10) were pooled per plot in each LUT and placed in individual trays. Ten trays with sterilized soil were used as control. The trays were watered regularly and checked for seedlings emergence fortnightly for 18 months. The experimental design used was 3×3 factorial experiments. ANOVA, Diversity index (H') and Similarity index (SI) were used to analyze the data. There was significant difference in seedling emergence among ecological zones and land use types (p<0.05). A total of 4,400 seedlings emerged from the soil samples. All species were distributed among 32 families. FL in the RF had the highest number of germinated seeds (705±37.33 seedlings) followed by DF in the RF (701±49.6 seedlings). The lowest emergence was in NF of the SF (199±28.41 seedlings). DF in the RF had highest number of species (34) distributed among 22 families. Emergence from soil seed bank of NF in ORW was generally with more of tree species than herbs that were predominant in FL and DF.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.96-96
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• 2020

Soybean seeds are important sources of non-nutritive health promoting metabolites. The contents of these metabolites are affected by both genetic and environmental factors. In the present study, the contents of five common isoflavones including daidzin, genistin, glycitin, malonyldaidzin, and malonylgenistin were analyzed in 72 soybeans of different seed coat colors, diversity, and of different origins including China (22), Japan (9), USA (12), India (4), and Korea (25). The average total isoflavone content (TIC) was maximum in Indian soybeans (3302.36 ㎍/g) and minimum in Chinese landraces (1214.95 ㎍/g). The Korean landraces had higher average TIC (2148.05 ㎍/g) than the USA genetic materials (1580.23 ㎍/g) and Japanese landraces (1485.99 ㎍/g). The content of malonylgenistin was in the range of 54.31 - 2385.68 ㎍/g in the entire population, and was the most abundant isoflavone irrespective of origin although there was content variation among individual soybeans. Besides, glycitin was the least concentrated isoflavone, and its content ranged from 0.00 to 79.79 ㎍/g. With respect to seed coat color, green soybeans from all countries displayed the maximum malonylgenistin and TIC contents. Exceptions were those of Korean and Indian origins where black and yellowish-green soybeans presented the highest malonylgenistin and TIC contents, respectively. In multivariate analysis, 92.72% of the variance was explained by the first two principal components, and the soybeans were grouped in to three clusters based on isoflavone contents. Overall, our findings signify the importance of seed coat color and origin as discriminant parameters, and provide wide spectrum of routes for breeding soybean cultivars.

• Journal of Plant Biology
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• v.13 no.1
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• pp.56-64
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• 1970

The present experiments differning from that conducted by agriculturists with the first consideration of food or seed production were performed in order to investigate the density effect on dry matter production in the artified population of maize. Three experimental plots were established and one seed was sown in the first plot, 2 seeds in the second plot and 3 seeds in the third plot, respectively. The space of each ridge was designed with 30cm in width and 50cm in length. The space of each seed was 4cm and the growth amount by density was measured through three times. The results obtained are as follows: 1) The total growth amount per unit area was in the order of the third, second and first plots until the second sampling. At the third sampling the whole plots showed almost the same growth amount except the first plot which showed a slightly smaller amount. 2) The growth amount per individual plant was in the order of the third, second and first plots until the second sampling, while the first plot showed drastic growth compared with the other two plots at the third sampling. 3) The growth amount by each organ(leaf, stem and root) of the plant body was markedly abundant in the stem of the non-productive part compared with that in the leaf of the productive part. Especially the root was affected remarkably by density and the first plot showed an abundant growth in comparison with the other plots which were too poor to resist the gound surface.

• Korean Journal of Agricultural and Forest Meteorology
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• v.21 no.4
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• pp.269-276
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• 2019

A semi-greenhouse experiment with field grown soybean (Glycine max L. cv Deawon, Uram, Jinpong, Soyon, Pungsangnamul, and Haewon) was performed in 2018. The experiment was aimed to investigate individual impacts of drought stress on soybean characteristic and seed yield. The three treatments were used in non-water stress (Control) during the soybean growth season, vegetative stage stress (VS), and flowering period stress (FS). Leaf number, LAI, leaf nitrogen concentration, and leaf biomass were decreased by drought at R4. In our study, the number of pods was 33.6% and 40.5% lower, respectively, in control than in VS and FS. In 100 seed weight, was 16.1% and 10.1% lower, respectively, in control than in VS and FS at R8. As a result, seed yield was 39.8% and 45.1% lower, respectively, in control than in VS and FS. Depending on the drought period, Daewon and Haewon showed a large decrease in yield, while Soyon did not change. The results of this study showed that flower and beginning pod setting stage responded more sensitively to the drought period than vegetative stage. Overall, these results demonstrate soybean seed yield formation more sensitive the during the flowing and beginning pod setting stage. We conclude that adequate water supply for pod setting stage, guaranteeing a high seed yield.

• Korean Journal of Agricultural and Forest Meteorology
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• v.12 no.3
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• pp.207-216
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• 2010

Individual differences of ozone ($O_3$) resistance for seed production, seed germination and seedling development were examined in this study. Five in each healthy and damaged trees of Pinus thunbergii growing in air polluted area for 12 years were chosen based on visible foliar injury and growth. The cones of P. thunbergii, which were collected from healthy and damaged trees, were analyzed for physical characteristics and seeds from the cones were used to test germination percentage under $O_3$ treatment. The germinated seeds were continuously exposed to $O_3$ treatment and the lipid peroxidation and activities of antioxidative enzymes were determined for both seeds and seedlings. The $O_3$ treatment for seed germination and seedling development were conducted at three conditions: control, 150 ppb and 300 ppb of $O_3$. The non-treated seeds from the damaged trees showed 21.6% lower germination than those from the healthy ones. On the $O_3$ treatment of 300 ppb, seed germination decreased approximately 10% for the healthy trees and 19% for the damaged trees compared to that on the control. The seeds from the healthy trees showed significantly higher activities of superoxide dismutase (SOD), glutathione reductase (GR), and catalase (CAT) than those from the damaged trees. The activities of GR, ascorbate peroxidase (APX), and CAT decreased along with the increasing $O_3$ concentration in two tree grades. Malondialdehyde (MDA) content of seeds was not influenced by $O_3$ treatment for two tree grades. In seedling development, there were no significant differences for length and biomass of needle and root of two tree grades at both the control and 150 ppb of $O_3$. At 300 ppb of $O_3$ treatment, however, the length and biomass of needle and stem decreased for two tree grades but no significant differences was detected in root. The seedlings from the damaged trees were more sensitive to the $O_3$ treatment, showing higher activities of SOD, APX, and CAT and content of MDA compared to those from the healthy tree seedlings. Our results indicate that seed germination and seedling development are vulnerable to increasing $O_3$ concentrations and that attention must be paid to the individual selection of tree species for reforestation.

• Korean Journal of Food Science and Technology
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• v.31 no.6
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• pp.1679-1682
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• 1999

This study was carried out to investigate the effect of hot taste of red pepper seed (RPS) extracts in terms of mixing ratio with red pepper powder (RPP) extracts. As the mixing ratio of RPP extracts to RPS extracts increased, the solid yield, viscosity, turbidity and Hunter a and b values were increased but the L values was decreased. The time-intensity curve from sensory evaluation showed that the hot taste of RPP extracts reached maximum point in short time and decreased fastly while that of RPS slowly developed and maintained the intensity with a little decrease in the tested time. The mixing ratio of RPP and RPS responded the properties of their individual hot taste.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.821-832
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• 1997

The aim of this study was to separate or purify some bioactive compounds from flowers of alfalfa(Medicago sativa L.) and to test of the isolated compounds on alfalfa for their autotoxicity and on Echinochloa crus-galli for their allelopathy for seed germination and seedling weight. Using thin layer chromatography(TLC) of $CHCl_3$ extracts, the most inhibitory band to alfalfa seed germination was determined. Germination inhibition of this extract suggested a complex chemical interaction. Separation and purification of compounds with CHCl$_3$ extract of fresh alfalfa flowers were conducted by a silica gel TLC, and microcrystalline cellulose TLC(MCTLC), followed by droplet countercurrent chromatography(DCCC) bioassay. Preliminary identification was done by high perfomance liquid chromatography(HPLC) on the most inhibitory fractions in DCCC. Ferulic acid, caffeic acid, vanillic acid, rutin, narringin were identified in fraction 5 and ferulic acid, caffeic acid, vanillic acid, rutin, coumarin in fraction 6. The phytotoxicity of their individual compound was tested on alfalfa and Echinochloa crus-galli seed germination and seedling weight. Coumarin and ferulic acid showed the most inhibitory effect on alfalfa seed germination and Echinochloa crus-galli seedling fresh and dry weight. These compounds may be, at least in part, involved in autotoxicity and allelopathy.

• Journal of Aquaculture
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• v.12 no.3
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• pp.193-196
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• 1999

In order to obtain the basic data for the effective seed production of Ureshis unicinctus sediment preference, burrow shape and burrowing depth in sediment were investigated in the laboratory. The highest valus in both sediment preference and burrowing rate of U. unicinctus were shown at fine and (0.11~0.50mm in the mean diameter). U. unicinctus made various types of burrows, such as J, L, S and U shapes. Generally only one individual inhabits in burrow with head-up.

• Korean Journal of Environment and Ecology
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• v.16 no.1
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• pp.87-93
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• 2002

The tree of heaven naturalized plant grows in the various habitats of the city. The principal reason is that it is reproduced not only through sexual reproduction but through asexual reproduction. By the spreading of sucker and a bud appearance of the seed it forms a group easily inside the city habitat. The distribution of seed and spreading of the seedlings of the tree of heaven were investigated like the followings. As the result of the sexual reproduction by the seed and hangs in cluster farm, 350 individuals are germinated at the Namsan the best environment, and 50 individuals at Toegyewon Rotary These seeds are dispersed by the effect of wind and geographical factor. Because the northwest wind is blowing mainly from late autumn to early spring, it is dispersed in the south. In Toegyewon Rotary, a slant site, is dispersed mainly by down the site. Compared with width of crown, the seedling of a seed germination are distributed outside more than inside the crown and the seed is spreaded until the maximum 7.5 times of crown width at the investigated area. 1 year seedlings appeared as a group at the place of 2-4 times of the crown (in 20m from the crown, 75% distribution). Considering the distribution by the seed scattering of the tree of heaven, there is a possibility of sudden individual increase even in the deteriorated city environment. So this possibility shows that we need to consider the protection of the city soil, and the species variety decrease resulted from the successful competition to the other species.

• Journal of Korean Society of Forest Science
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• v.90 no.1
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• pp.155-160
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• 2001

Changes in genetic diversity after seed-tree practice were simulated in test plantation of tulip tree at Sokdal-ri, Banwol-myon, Hwasung-gun, Kyungki Provence. I-SSR markers were analyzed in a total of 305 tulip trees. A total of 89 amplicon variants were observed by PCR with 9 I-SSR primers. Genetic diversity for 305 trees was relatively high (S. I. =0.4532). Individuals originated from the seed orchards in U.S.A. showed the highest level of genetic diversity (0.4530), those from Anyang showed the medium level (0.4152), and those from Cheonbuk showed the lowest (0.3929). Simulation of seed-tree practice accompanied by 2 consecutive thinnings was performed on the basis of morphological characteristics and planted distances of the individual trees, which left 37 trees as candidates for seed-trees. Decreasing rate of genetic diversity within seed sources was greatest for individuals from Cheonbuk (28.3%), moderate for those from Anyang (16.3%), and smallest for those from U.S.A. (8.0%). In spite of little difference in decreasing rate of individuals for the 3 seed sources (87.5~88.2%), large difference in decreasing rate of genetic diversity within seed sources might be due to difference in number of mother trees for the 3 seed sources. For example, whereas individuals originated from the seed orchard in U.S.A., which showed the smallest decreasing rate of genetic diversity, might be originated from relatively large number of mother trees, those from Anyang and Cheonbuk might be originated from relatively small number of mother trees. Although mean of 17.5% of the genetic diversity within seed sources was decreased through 2 consecutive thinnings, a decrease in genetic diversity for the whole individuals (37 trees) was only 6.1%. This observation suggests that the seed-tree practice on the basis of the criteria established in the present study may not result in great reduction in overall genetic diversity of the progenies.