• BMB Reports
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• v.51 no.9
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• pp.437-443
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• 2018

Non-homologous end joining (NHEJ), and to a lesser extent, the error-free pathway known as homology-directed repair (HDR) are cellular mechanisms for recovery from double-strand DNA breaks (DSB) induced by RNA-guided programmable nuclease CRISPR/Cas. Since NHEJ is equivalent to using a duck tape to stick two pieces of metals together, the outcome of this repair mechanism is prone to error. Any out-of-frame mutations or premature stop codons resulting from NHEJ repair mechanism are extremely handy for loss-of-function studies. Substitution of a mutation on the genome with the correct exogenous repair DNA requires coordination via an error-free HDR, for targeted transgenesis. However, several practical limitations exist in harnessing the potential of HDR to replace a faulty mutation for therapeutic purposes in all cell types and more so in somatic cells. In germ cells after the DSB, copying occurs from the homologous chromosome, which increases the chances of incorporation of exogenous DNA with some degree of homology into the genome compared with somatic cells where copying from the identical sister chromatid is always preferred. This review summarizes several strategies that have been implemented to increase the frequency of HDR with a focus on somatic cells. It also highlights the limitations of this technology in gene therapy and suggests specific solutions to circumvent those barriers.

• Molecules and Cells
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• v.26 no.1
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• pp.5-11
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• 2008

Stalled DNA replication forks activate specific DNA repair mechanism called post-replication repair (PRR) pathways that simply bypass DNA damage. The bypassing of DNA damage by PRR prevents prolonged stalling of DNA replication that could result in double strand breaks (DSBs). Proliferating cell nuclear antigen (PCNA) functions to initiate and choose different bypassing pathways of PRR. In yeast, DNA replication forks stalled by DNA damage induces monoubiquitination of PCNA at K164, which is catalyzed by Rad6/Rad18 complex. PCNA monoubiquitination triggers the replacement of replicative polymerase with special translesion synthesis (TLS) polymerases that are able to replicate past DNA lesions. The PCNA interaction motif and/or the ubiquitin binding motif in most TLS polymerases seem to be important for the regulation of TLS. The TLS pathway is usually error-prone because TLS polymerases have low fidelity and no proofreading activity. PCNA can also be further polyubiquitinated by Ubc13/ Mms2/Rad5 complex, which adds an ubiquitin chain onto monoubiquitinated K164 of PCNA. PCNA polyubiquitination directs a different PRR pathway known as error-free damage avoidance, which uses the newly synthesized sister chromatid as a template to bypass DNA damage presumably through template switching mechanism. Mammalian homologues of all of the yeast PRR proteins have been identified, thus PRR is well conserved throughout evolution. Mutations of some PRR genes are associated with a higher risk for cancers in mice and human patients, strongly supporting the importance of PRR as a tumor suppressor pathway.

• Journal of the Korean Institute of Navigation
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• v.17 no.1
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• pp.91-98
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• 1993

This paper presents a class of Bayes estimation of component steady-state availability . Throughout this paper, we will denote the mean time between failure and the mean time between repair by MTBF and MTBR respectively. In section 2 , we investigated Bayes estimation of the steady-state availability for noninformative prior density function and in section 3, we compute Bayes estimation for conjugate prior density function.

• Journal of Chest Surgery
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• v.56 no.6
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• pp.456-459
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• 2023

Penetrating chest trauma may result in significant intracardiac injury. A traumatic ventricular septal defect is a rare complication that requires surgical management, particularly if heart failure ensues. We report a case of delayed repair of an outlet-type ventricular septal defect and perforation of the aortic and pulmonary valve leaflets following a stab wound. This report highlights diagnostic and surgical considerations and also presents an opportunity to review the conotruncal anatomy, which may be relatively unfamiliar to many adult cardiac surgeons.

• Proceedings of the Korea Concrete Institute Conference
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• 1996.04a
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• pp.323-327
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• 1996

Reinforced concrete(R/C) structures need repair and rehabilitation due to the deterioration such as a crack, spalling and disintergration. Numerous repair materials which are currently used in construction fields without any regulation are examined in terms of their serviceabilities and effectiveness. In this paper section of existing R/C beams are enlarged with repair materials, that is, epoxy, latex or premix. And then they are strengthened with rebar, steel poate of CFRP on the tension face. Structural behaviors of strengthened beams are investigated both statically and dynamically and they are compared with each. This paper summarizes the overall research plan.

• Proceedings of the Korea Concrete Institute Conference
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• 2000.04a
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• pp.733-738
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• 2000

This paper was aimed to evaluate the structural performance of flexural members repaired by polymer cement and epoxy mortar at soffit. Main test variables were repair materials, ratio of reinforcement and additional reinforcing bars. Test results shows that the repaired beams could change flexural capacity by materials and additional reinforcing bars. In polymer cement, the section repaired can carry same load, cracking moment and the flexural stiffness of the monolithic beams with same size. In epoxy mortar, all data were greater than the shotcrete. However, note that epoxy mortar may conduct member into brittle failure mode.

• Proceedings of the KWS Conference
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• 2007.11a
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• pp.196-198
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• 2007

Laser cladding technology was studied as a method for upgrading the present repair procedures of damaged tubes in a nuclear steam generator and Doosan subsequently developed and designed a new Laser Cladding Repair System. One of the important features of this newly developed Laser Cladding Repair System is that molten metal can be deposited on damaged tube surfaces using a laser beam and filler wire without the need to install sleeves inside the tube. Laser cladding qualification tests on the steam generator tube material, Alloy 600, were performed according to ASME Section IX.

• Computers and Concrete
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• v.15 no.1
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• pp.73-88
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• 2015

Following previous studies, the current paper describes the results of an experimental program concerning the repair of reinforced concrete columns by thixotropic pseudo plastic mortar, preformed to analyze and quantify the influence of initial construction damage to the behavior of the repaired element. Five columns (section scale 1:2) were designed according to the minimum requirements of reinforcement of ductility orientated codes' design with variables the percentages of initial construction damages. All were tested in axial compression with repeated cycles up to failure. For comparison reasons, another one of the same characteristics, yet healthy, was constructed and tested as a reference specimen. A numerical study (Finite Element Analysis) was conducted for further investigation of the behavior of the thixotropic mortar as repair material. The results indicate that: a) surpassing a specific amount of damage, columns even suitably repaired present lower strain capacity, b) finite element analysis present the same way of deboning of the repaired material taking into consideration the buckling of the reinforcement bars.

• International Journal of Safety
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• v.10 no.1
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• pp.16-21
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• 2011

In this thesis, appearance inspection, compressive strength of concrete test, arrangement of bar inspection, survey, and bearing stress analysis were performed on a damaged coping of reinforced concrete pier to investigate the damage cause. According to the performed a series of inspections, it was found that the coping of pier was damaged during PSC (Pre-stressed Concrete) beam construction. In this thesis, the repair method for damaged pier was studied. The repair procedure used in this thesis was follows : chipping for damaged part, clean by high-pressure, installation of wire mesh, coating of surface hardening, construction of section restoration material, copula grinding, and prevent coating for far-infrared radiation.

• Journal of Korean Association for Spatial Structures
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• v.9 no.3
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• pp.93-100
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• 2009

As interest in latest cultural heritance is increased, an importance about conservation of dilapidated cultural building asset is risen. Most of cultural building asset used wood by main material and conservation repair about corrosion and damage by deterioration of these cultural building asset considers cultural value of member and the basis principle is the archetype maintenance. Accordingly, conservation processing method that use synthetic is embossed. This paper experimented with reinforcement sectional area ratio, direction of section, length, strength of synthetic resins as variable and manufactures total 14 specimens as experimental study about compression reinforcement performance of circular section wood that strengthen by synthetic resins. The result of this paper has shown that adequate strengthen is more efficient than new member and the most important authenticity in strengthen and repair of cultural heritance can be ensured.