• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.245-246
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• 2000

Agrobacterium sp. ATCC 31750( formerly Alcaligenes faecalis subsp myxogenes) was used to produce curdlan. Since the curdlan is secondary metabolite, it is important for curdlan production to increase cell concentration. The fedbatch operation was used to increase cell concentration with addition of carbon and nitrogen sources. When the initial sucrose concentration was 20g/L, it was consumed in 24 hrs and the cell concentration was 6g/L in a batch culture. The sucrose solution(200g/L) was fed to control the sucrose concentration above 10g/L.

• International Journal of Oral Biology
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• v.45 no.2
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• pp.64-69
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• 2020

Short-chain fatty acids (SCFAs) such as acetate, propionate, and butyrate are secondary metabolites produced by anaerobic fermentation of dietary fibers in the intestine. Intestinal SCFAs exert various beneficial effects on intestinal homeostasis, including energy metabolism, autophagy, cell proliferation, immune reaction, and inflammation, whereas contradictory roles of SCFAs in the oral cavity have been reported. Herein, we found that low and high concentrations of SCFAs induce differential regulation of intracellular Ca²⁺ mobilization and expression of pro-inflammatory cytokines, such as interleukin (IL)-6 and IL-8, respectively, in gingival fibroblast cells. Additionally, cell viability was found to be differentially regulated in response to low and high concentrations of SCFAs. These findings demonstrate that the physiological functions of SCFAs in various cellular responses are more likely dependent on their local concentration.

• Plant Biotechnology Reports
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• v.2 no.2
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• pp.133-136
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• 2008

Cell cultures of Commiphora wightii (Arnott.) Bhandari were grown in shake flasks and a bioreactor and an increase in guggulsterone accumulation up to $18{\mu}g\;l^{-1}$ was recorded in cells grown in the production medium containing a combination of sucrose:glucose (4% total), precursors (phenylalanine, pyruvic acid, xylose, and sodium acetate), morphactin, and 2iP. A yield of $10g\;l^{-1}$ biomass and ${\sim}200{\mu}g\;l^{-1}$ guggulsterone was recorded in a 3-l flask and in a 2-l stirred tank bioreactor compared with 6.6 g biomass and $67{\mu}g\;l^{-1}$ guggulsterone in 250-ml flasks. Increased vessel size was correlated with increased biomass and guggulsterone accumulation. 2iP alone was not effective for biomass and guggulsterone accumulation in cell cultures of C. wightii.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.367-367
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• 2011

CIGS solar cell with copper, indium, gallium and selenium is a second generation solar cells for the lowering of the manufacturing cost. The relative ratio of the four elements is one of the most important measurement issues because the photovoltaic property of CIGS solar cell depends on the crystalline structure of the CIGS layer. However, there is no useful analysis method for the composition of the CIGS layer. Recently, AES depth profiling analysis of CIGS films has been studied with a reference material certified by inductively coupled plasma optical emission spectroscopy. However, there are some problems in AES depth profiling analysis of CIGS films. In this study, the in-depth profiling analysis was investigated by secondary ion mass spectrometry (SIMS) depth profiling analysis. We will present the compositional depth profiling of CIGS films by SIMS and its applications for the development of CIGS solar cells with high efficiency.

• Proceedings of the KIPE Conference
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• 2004.11a
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• pp.124-126
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• 2004

This paper describes a boost converter cascaded high frequency link direct do-ac converter suitable for fuel cell power sources. A new multi-loop control for a boost converter to reduce the low frequency input current harmonics drawn from fuel cell is proposed. A new PWM technique for the cycloconverter at the secondary to reject the low order harmonics in the output voltages is presented in detail.

• Archives of Pharmacal Research
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• v.29 no.1
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• pp.59-63
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• 2006

Secalonic acid D(SAD) was isolated as the major secondary metabolite of the marine lichen-derived fungus Gliocladium sp. T31. Its structure was established on the basic of physicochemical and spectroscopic data. This is the first report on the isolation of SAD from this fungus, as well as its inhibitory effect on K562 cell cycle and its cytotoxicity against several tumor cell lines in vitro.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2005.11a
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• pp.245-249
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• 2005

This paper describes a boost converter cascaded high frequency link direct dc-ac converter suitable for fuel cell power sources. A new multi-loop control for a boost converter to reduce the low frequency input current harmonics drawn from fuel cell is proposed. A new PWM technique for the cycloconverter at the secondary to reject the low order harmonics in the output voltages is presented in detail.

• Proceedings of the KIPE Conference
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• 2007.07a
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• pp.350-352
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• 2007

Modularized charge equalization converter for HEV lithium-ion battery cells is proposed in this paper, in which intra-module and inter-module charge equalization can be achieved at the same time. For intra-module charge equalization, the conventional flyback DC/DC converters of low power and small size are employed, in which all of the primary sides are coupled in parallel for selective charge of the specific under charged cell within the module. For inter-module charge equalization, the flyback DC/DC converters are also added, in which all the secondary windings are electrically linked in parallel for automatic charge balancing among the modules. An engineering sample of forty cells hiring the proposed cell balancing scheme is implemented and its experimental result shows that the proposed modularized charge equalization circuit has good cell balancing performance.

• Nuclear Engineering and Technology
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• v.51 no.4
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• pp.996-1007
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• 2019

We investigated the influence of the aspect ratio (H/R) of the oxide layer on the reactor vessel heating in three-layer configuration. Based on the analogy between heat and mass transfers, we performed mass transfer experiments to achieve high Rayleigh numbers ranging from $6.70{\times}10^{10}$ to $7.84{\times}10^{12}$. Two-dimensional (2-D) semi-circular apparatuses having the internal heat source were used whose surfaces of top, bottom and side simulate the interfaces of the oxide layer with the light metal layer, the heavy metal layer, and the reactor vessel, respectively. Multi-cell flow pattern was identified when the H/R was reduced to 0.47 or less, which promoted the downward heat transfer from the oxide layer and possibly mitigated the focusing effect at the upper metallic layer. The top boundary condition greatly affected the natural convection of the oxide layer due to the presence of secondary flows underneath the cold light metal layer.

• Korean Journal of Transplantation
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• v.28 no.3
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• pp.113-120
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• 2014

Two-signal models are useful in explaining various types of immune responses. In particular, secondary, so-called costimulatory, signals are critically required for the process of T-cell activation, survival, differentiation, and memory formation. Early studies in rodent models showed that targeting T-cell costimulatory pathways elicits immunological tolerance, providing a basis for development of costimulatory therapeutics in allograft rejection. However, as the classic definition of T-cell costimulation continues to evolve, simple blockade of costimulatory pathways has limitations in prevention of allograft rejection. Furthermore, functions of costimulatory molecules are much more diverse than initially anticipated and beyond T cells. In this mini-review, we will discuss CD137-CD137L bidirectional signals as examples showing that two-signals can be applicable to multiple phases of immune responses.