• Title/Summary/Keyword: Secondary Cell

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Effects of Quercetin on the Immune Responses in Mice (Quercetin이 마우스의 면역반응에 미치는 영향)

  • 안영근;박영길;김정훈
    • YAKHAK HOEJI
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    • v.35 no.5
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    • pp.401-415
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    • 1991
  • Effects of quercetin on the specific and non-specific immune responses were studied in vivo. Quercetin at a dose of 2.5, 5, 10, 20 and 40 mg/kg were orally administered to ICR male mice once daily for 28 consecutive days. Cyclophosphamide was injected intraperitoneally to ICR mice with a single dose of 5 mg/kg 2 days before secondary immunization. Mice were sensitized and challenged with sheep red blood cells (S-RBC). Immune responses were evaluated by humoral and cellular immune reponses and non-specific immune response. The results of this study were summarized as followings; 1. Quercetin significantly decreased the body weight, and introduced the atrophy of liver, spleen and thymus gland dose-dependently, but increased the numbers of white blood cell. 2. Querectin significantly depressed the hemagglutination titer, Arthus reaction and hemolytic plaque forming cell. 3. Quercetin significantly depressed the delayed type hypersensitivity and rosette forming cell. 4. Quercetin at a dose of 2.5, 5 and 40 mg/kg significantly depressed phagocytic activity. 5. Quercetin at a dose of 10 and 20 mg/kg significantly increased natural killer cell activity.

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Similarities of Tobacco Mosaic Virus-Induced Hypersensitive Cell Death and Copper-Induced Abiotic Cell Death in Tobacco

  • Oh, Sang-Keun;Cheong, Jong-Joo;Ingyu Hwang;Park, Doil
    • The Plant Pathology Journal
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    • v.15 no.1
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    • pp.8-13
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    • 1999
  • Hypersensitive cell death of plants during incompatible plant-pathogen interactions is one of the efficient defense mechanisms of plants against pathogen infections. For better understanding of the molecular mechanisms involved in the plant hypersensitive response (HR), TMV-induced biotic plant cell death and CuSO4-induced abiotic plant cell death were compared in terms of expression patterns of ten different defense-related genes as molecular markers. The genes include five pathogenesis-related protein genes, two plant secondary metabolite-associated genes, two oxidative stress-related genes and one wound-inducible gene isolated from tobacco. Northern blot analyses revealed that a same set of defense-related genes was induced during both biotic and abiotic cell death but with different time and magnitude. The expression of defense-related genes in tobacco plants was temporarily coincided with the time of cell death. However, when suspension cell cultures was used to monitor the expression of defense-related genes, different patterns of the gene expression were detected. This result implies that three are common and, in addition, also different branches of signaling pathways leading to the induced expression of defense-related genes in tobacco during the pathogen- and heavy metal-induced cell death.

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Effects of Reynolds Number on Flow and Heat/Mass Characteristics Inside the Wavy Duct (Reynolds 수에 따른 꺾어진 덕트에서 열/물질전달 특성 고찰)

  • 장인혁;황상동;조형희
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.15 no.10
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    • pp.809-820
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    • 2003
  • The present study investigates effects of flow velocity on the convective heat/mass transfer characteristics in wavy ducts of a primary surface heat exchanger application. Local heat/mass transfer coefficients on the wavy duct sidewall are determined by using a naphthalene sublimation technique. The flow visualization technique is used to understand the overall flow structures inside the duct. The aspect ratio and corrugation angle of the wavy duct is fixed at 7.3 and 145$^{\circ}$ respectively, and the Reynolds numbers, based on the duct hydraulic diameter, vary from 100 to 5,000. The results show that there exist complex secondary flows and transfer processes resulting in non-uniform distributions of the heat/mass transfer coefficients on the duct side walls. At low Re (Re<1000), relatively high heat/mass transfer regions like cell shape appear on both pressure and suction side wall due to the secondary vortex flows called Taylor-Gortler vortices perpendicular to the main flow direction. However, at high Re (Re>1000), these secondary flow cells disappear and boundary layer type flow characteristics are observed on pressure side wall and high heat/mass transfer region by the flow reattachment appears on the suction side wall. The average heat/mass transfer coefficients are higher than those of the smooth circular duct due to the secondary flows inside wavy duct. And also friction factors are about two times greater than those of the smooth circular duct.

Somatic Embryogenesis and Plant Regeneration in Embryogenic Cell Suspension Cultures of Hovenia dulcis Thunb (헛개나무의 현탁배양세포로부터 체세포배발생과 식물체 재생)

  • Li, Cheng-Hao;Zhao, Bo;Kim, Na-Young;Kim, Myong-Jo;Cho, Dong-Ha;Lee, Dong-Wook;Lee, Jae-Geun;Lim, Jung-Dae;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.4
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    • pp.255-260
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    • 2006
  • Culture conditions for high frequency plant regeneration via somatic embryogenesis from embryogenic cell suspension cultures of Hovenia dulcis are described. Germinated somatic embryos were selected for induction of secondary embryogenesis. Friable embryogenic cells were induced directly from somatic embryos when transfer to 1/3 MS solid or liquid medium lacking plant growth regulators. The temperature strongly effected on induction of secondary embryognesis than other conditions in culture. All somatic embryos produced friable embryogenic cell clumps within 10 days when germinated somatic embryos cultured in 1/3 MS medium at $30^{\circ}C$ in suspension culture. No somatic embryos formed from embryogenic cell suspension cultures at $18^{\circ}C$. Numerous somatic embryos were induced and subsequently developed uniformly into germination stage from suspended cell clumps after 4 weeks of culture on $18^{\circ}C$. Plantlets conversion were observed on $18^{\circ}C$ when germinated somatic embryos were transferred to 1/3 MS solid medium without plant growth regulators or supplemented with 0.1-0.5 mg/l benzyladenine.

Berberine Production by Cell Suspension Cultures of Cork Tree (Phellodendron amurense Rupr)

  • Choi, Myung-Suk;Shin, Dong-Ill;Park, Young-Goo
    • Korean Journal of Pharmacognosy
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    • v.27 no.1
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    • pp.32-36
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    • 1996
  • Various culture conditions for cell growth and berberine production in cork tree (Phellodendron amurense Rupr.) were investigated. Callus was induced from cambium tissue of cork tree, and cultured on LS liquid medium supplemented with 0.5 mg/1 2,4-D, 0.1mg/1 BA, and 3% sucrose. Several factors enhancing berberine production and cell growth in cork tree cell cultures were found. Some of them enhanced both cell growth and berberine production, but others resulted in a decoupling of cell growth and berberine production with significant in the specific levels. High level of nitrate (80mM), high level of phosphate (8.98mM), and sucrose (7%), 1.0mg/l IAA were effective in berberine production, whereas low level of nitrate (40mM), and phosphate (2.25mM), and high level of sucrose (7%) in the medium were effective in cell growth. Two stage culture(first stage for cell growth, and second stage for berberine production) increased berberine production almost twice (5.06mg/g dry weight) as much as single stage cultures in berberine production.

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Primary Bronchial Granular Cell Tumor in an Adult Male

  • Kim, Ho Jin;An, Soyeon;Kim, Hyeong Ryul
    • Journal of Chest Surgery
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    • v.47 no.2
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    • pp.193-196
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    • 2014
  • We report a rare case of granular cell tumor arising in the left lower lobe (LLL) bronchus with secondary obstructive change in a 60-year-old male. The patient was found to have a nodule in the LLL on a computed tomography scan, three months prior to his presentation to the Asan Medical Center. Bronchoscopic biopsies revealed a granular cell tumor. After undergoing LLL lobectomy with bronchoplasty, the patient has not experienced any tumor recurrence.

Observation of Water Consumption in Zn-air Secondary Batteries

  • Yang, Soyoung;Kim, Ketack
    • Journal of Electrochemical Science and Technology
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    • v.10 no.4
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    • pp.381-386
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    • 2019
  • Zn-air battery uses oxygen from the air, and hence, air holes in it are kept open for cell operation. Therefore, loss of water by evaporation through the holes is inevitable. When the water is depleted, the battery ceases to operate. There are two water consumption routes in Zn-air batteries, namely, active path (electrolysis) and passive path (evaporation and corrosion). Water loss by the active path (electrolysis) is much faster than that by the passive path during the early stage of the cycles. The mass change by the active path slows after 10 h. In contrast, the passive path is largely constant, becoming the main mass loss path after 10 h. The active path contributes to two-thirds of the electrolyte consumption in 24 h of cell operation in 4.0 M KOH. Although water is an important component for the cell, water vapor does not influence the cell operation unless the water is nearly depleted. However, high oxygen concentration favors the discharge reaction at the cathode.

A case of nodal marginal zone B-cell lymphoma of the lower eyelid

  • Lee, Cho Long;Lee, Il Seok;Jung, Sung Gyun
    • Archives of Craniofacial Surgery
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    • v.21 no.4
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    • pp.249-252
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    • 2020
  • A marginal zone B-cell lymphoma is a type of B-cell lymphoma which is normally located at the margins of the secondary lymph node follicles. According to 2008 World Health Organization (WHO) classification, there are three main types classified according to the location of invasion: nodal lymphoma in the lymph nodes, splenic lymphoma in the spleen, and extranodal lymphoma in other locations. Recently, we have experienced a rare case of primary nodal marginal zone lymphoma that arose in the lower eyelid. Therefore, we report this case with a review of literature.

Effectcs of Plant Growth Regulators on Growth and Berberine Production in Cell Suspension Cultures of Thalictrum rugosum (Thalictrum rugosum 세포배양에서 식물생장 조절물질이 세포증식 및 Berberine 생산에 미치는 영향)

  • 김동일
    • Microbiology and Biotechnology Letters
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    • v.18 no.4
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    • pp.327-330
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    • 1990
  • The effects of various plant growth regulators, both auxins and cytokinins, on cell growth and berberine production were investigated in cell suspension cultures of Thafictrum rugosum. Indole-%-acetic acid (IAA) was found to be the best for berberine production among five examined plant growth regulators and the optimum concentration of IAA was 1 $\mu \textrm M$. The enhancement compared to control 2, 4-dichlorophenoxyacetic acid (2, 4-D) was more than 60%. Simultaneous addition of cytokinins such as kinetin and 6-benzylamiroyurine (BA) was inhibitory.

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Development and Validation Study of a Cell Phone Addiction Scale for Korean Children (아동용 휴대전화 중독 도구 개발 및 타당화 연구)

  • Koo, Hyun-Young;Yoo, Myung-Sook
    • Child Health Nursing Research
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    • v.18 no.2
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    • pp.76-84
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    • 2012
  • Purpose: This study was done to develop a cell phone addiction scale for Korean children. Methods: The process included construction of a conceptual framework, generation of initial items, verification of content validity, selection of secondary items, preliminary study, and extraction of final items. The participants were 268 children from grades 4 to 6 in two urban elementary schools. Construct validity, internal consistence reliability, and split-half reliability were used to analyze the data. Results: Nine items were categorized into 2 factors explaining 61.29% of total variance. The factors were labeled as obsessive compulsion (6 items) and dependency (3 items). Cronbach's alpha coefficient for the 9 items was .87, and Spearman-Brown coefficient was .83. Scale scores identified children as high risk users, at risk users, or average users by standard scores. Conclusion: The above findings indicate that the cell phone addiction scale for children has good validity and reliability when used with Korean children.