• Title/Summary/Keyword: Seaweeds addition

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Protective Activity of Fucoidan and Alginic Acid against Free Radical-Induced Oxidative Stress under in Vitro and Cellular System

  • So, Mi-Jung;Kim, Boh-Kyung;Choi, Mi-Jin;Park, Kun-Young;Rhee, Sook-Hee;Cho, Eun-Ju
    • Preventive Nutrition and Food Science
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    • v.12 no.4
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    • pp.191-196
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    • 2007
  • We investigated radical scavenging effects and protective activities of fucoidan and alginic acid, active polysaccharide components from brown seaweeds, against peroxyl radical-induced oxidative stress under in vitro and cellular system. Fucoidan exerted strong radical scavenging effects against nitric oxide (NO) and superoxide anion $(O_2)$. On the other hand, alginic acid did not show inhibitory activity against NO and relatively weak $O_2{^-}$ scavenging effect. Additionally, alginic acid exhibited higher hydroxyl scavenging activity than fucoidan. Both fucoidan and alginic acid significantly enhanced cell viability against oxidative stress induced by 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH). At $1000{\mu}g/mL$ concentration of fucoidan and alginic acid, the viability was increased from 16.4% to 85.9% and 67.7%, respectively. In addition, fucoidan and alginic acid ameliorated the lipid peroxidation in LLC-PK1 cell induced by AAPH in a dose-dependent manner. In particular, fucoidan showed stronger inhibitory effect than alginic acid in the cellular system. The present study suggests that fucoidan and alginic acid may be promising antioxidants against oxidative stress induced by free radicals.

The Consolidation Medium for the Conservation of Mud Wall Painting (토벽화 보존에 따른 고착제에 관한 연구)

  • Han, Kyeong Soon
    • Journal of Conservation Science
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    • v.11 no.1 s.14
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    • pp.38-51
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    • 2002
  • The first priority we face in the conservation of wall paintings, is the conservation of painting layer. The 'painting layer' is sometimes called the pigment layer, and refers to the painted surface. For the consolidation of this painting layer we use 'fixative'. Fixative is a common adhesive which has been used in various field and conservators have made up for the weak points in the consolidation of wall paintings. In my thesis, I will summarise the range of use, standards of selection, and results of research on the use of fixative in the conservation of European paintings. In addition, I will describe various kinds of our traditional fixatives. As synthetic resins have proved that it is not ideal as a fixative for wall painting, conservators have studied to find an alternative. Same as European conservators, Asian scientists has researched to find alternative fixative which is more suitable to the wall paintings in Asia based on their traditional techniques and materials. Therefore, 1 have studied to find an adequate fixative for our own wall paintings and I could conclude that we can consider traditional fixative which is made of 'seaweeds' as an alternative and it is very important to proceed research and experiment on this material.

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Polyphenol-rich fraction from Ecklonia cava (a brown alga) processing by-product reduces LPS-induced inflammation in vitro and in vivo in a zebrafish model

  • Kim, Seo-Young;Kim, Eun-A;Kang, Min-Cheol;Lee, Ji-Hyeok;Yang, Hye-Won;Lee, Jung-Suck;Lim, Tae Il;Jeon, You-Jin
    • ALGAE
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    • v.29 no.2
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    • pp.165-174
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    • 2014
  • Ecklonia cava is a common edible brown algae that is plentiful in Jeju Island of Republic of Korea. Polyphenols from E. cava have strong anti-inflammatory activity. However, a large number of the by-products from E. cava processing are discarded. In the present study, to utilize these by-products, we assessed the anti-inflammatory activity of the polyphenol-rich fraction (PRF) from E. cava processing by-product (EPB) in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells. Four compounds, namely eckol, eckstolonol, dieckol, and phlorofucofuroeckol-A, were isolated and identified from PRF. We found that PRF suppressed the production of nitric oxide (NO), inducible nitric oxide synthase, and cyclooxygenase-2 in the LPS-induced cells. Furthermore, the protective effect of PRF was investigated in vivo in LPS-stimulated inflammation zebrafish model. PRF had a protective effect against LPS-stimulated toxicity in zebrafish embryos. In addition, PRF inhibited LPS-stimulated reactive oxygen species and NO generation. According to the results, PRF isolated from EPB could be used as a beneficial anti-inflammatory agent, instead of discard.

Anti-inflammatory Effects of Enzymatic Extract from Ecklonia cava on TPA-induced Ear Skin Edema

  • Ahn, Ginnae;Park, Eun-Jin;Kim, Dae-Seung;Jeon, You-Jin;Shin, Tae-Kyun;Park, Jae-Woo;Woo, Ho-Chun;Lee, Ki-Wan;Jee, Young-Heun
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.745-750
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    • 2008
  • Anti-inflammatory potential of the enzymatic extract prepared by Kojizyme (ECK), a component of brown seaweeds Ecklonia cava (Alariaceae, Phaeophyta) in vivo was investigated. For the application of mouse ear edema model, 12-O-tetradecanoylphorbol acetate (TPA) was used, a topical inducer of a long-lasting inflammatory response. Our results demonstrated that ECK inhibited ear edema when topically applied to mouse ear skin. In histological evaluation, the inhibition activity of ECK on TPA-induced inflammation is similar to that of dexamethasone, although less strong. In addition, the mRNA expression levels of IL-$1{\beta}$, IFN-$\gamma$, TNF-$\alpha$, and cyclooxygenase-2 (COX2) and the immunoreactivity to inducible nitric oxide synthase (iNOS) and COX2 expressed mainly in inflammatory cells were down-regulated by ECK. These results indicate that ECK has anti-inflammatory effects through the inhibition of Th1 cytokines and 2 inducers of inflammation in TPA-induced ear skin edema.

A Study on the Dyeability of Natural Dyes of Sustainable Seaweed-Containing Fiber (지속 가능한 해초 함유 섬유의 천연염료 염색성에 관한 연구)

  • Kim, Sojin;Choi, Kyoungmi
    • Journal of Fashion Business
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    • v.26 no.3
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    • pp.87-97
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    • 2022
  • This study investigated the dyeability and color change of the natural dyes of SeaCell, a biodegradable functional fiber that is permanently added to cellulose fibers with natural additives extracted from seaweeds. The natural dyes used in the study are five dyes. Gardenia and turmeric, which are yellow-based natural dyes, Sappan wood and Lac, which are red-based natural dyes, and Indigo, a blue-based natural dye, were selected. The dyeability and color change according to the change of the mordant conditions and the number of times of dyeing were investigated. In addition, the dyeing properties and colors of cotton and silk fibers were compared under the same dyeing conditions as SeaCell. The study results are as follows. It was found that SeaCell had lower dyeing properties than silk, a protein fiber, in gardenia, sappan wood, and lac dyes, but had higher dyeing properties than cotton with the same cellulose component as SeaCell fibers. In the case of turmeric, it showed higher dyeing properties than cotton except for the no mordant condition. In the case of Indigo dye, SeaCell shows the best dyeability, indicating that it is a very suitable fiber for Indigo dyeing. As sustainable functional fibers are continuously developed in the future, natural dyes that are environmentally friendly and human-friendly are actively introduced and commercialized, and it is expected that they can be used as reference materials.

Behavioral analysis of Pacific abalone, Haliotis discus hannai, reveals its feeding preference and attraction potential for brown alga, Sargassum horneri

  • Chae-Eun Yu;Yeo-Reum Kim;Gyeong-Eon Noh;Jong-Myoung Kim
    • Fisheries and Aquatic Sciences
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    • v.26 no.5
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    • pp.355-365
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    • 2023
  • The Pacific abalone, Haliotis discus hannai, is a highly valued and industrially important aquaculture species with growing demands of the expanding abalone aquaculture industry. To explore the feasibility of using the brown alga, Sargassum horneri, as a potential substitute for abalone feed, it is important to identify the feed preference and attractant effect of S. horneri on Pacific abalone. Our experiments indicated that the feeding-associated movement of abalone could be detected using a video tracking system under indirect illumination with dim red light. To further analyze the attraction potentials of various test materials, preference analysis was performed using Avicel-coated glass plates with ground powders of various seaweeds (e.g., S. horneri, Saccharina japonica, and Undaria pinnatifida) and commercial abalone feed, together with coffee waste. Heat map analysis indicated greater attraction by the kelp S. japonica than by S. horneri and commercial feed, which showed similar preference levels. Feeding preference based on the area of Avicel eaten by abalone showed a significant preference for U. pinnatifida over S. horneri (feeding area: 68.6 ± 20.1% vs. 37.5 ± 22.4%, p < 0.05). Additionally, the feeding area was significantly greater for plates with S. japonica than for plates with S. horneri (44.0 ± 16.6% vs. 22.6 ± 15.4%, p < 0.05). There was no significant difference in feeding area between commercial feed and S. horneri (31.7 ± 11.6% vs. 31.6 ± 20.2%, p > 0.05). The methanol extracts attracted abalone in the following order: U. pinnatifida > S. horneri > S. japonica > commercial feed > coffee waste. To determine the attractive effects of the components of methanol extracts, mixtures of methanol extracts of commercial feed with increasing amounts of S. horneri were examined. The results showed a significant increase in feeding preference upon addition of S. horneri up to 50% and 75%, suggesting its potential for use as an appetite-enhancing feed additive. This study identified conditions that can be successfully used to monitor the movement of Pacific abalone; the results of preference analysis confirmed that abalone exhibited similar attraction and feeding preference for S. horneri, compared with commercial feed.

Comparison of Food Intakes and Serum Lipid Levels in Overweight and Obese Women by Body Mass Index (과체중 및 비만여성의 식습관, 식이섭취실태 및 혈청지질 양상 비교)

  • Kim, Ok-Hyun;Jung, Ha-Na;Kim, Jung-Hee
    • Korean Journal of Community Nutrition
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    • v.12 no.1
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    • pp.40-49
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    • 2007
  • This study was done to investigate the health-related habits, dietary intakes and serum lipid levels in overweight and obese women by body mass index(BMI). Subjects were 137 pre-menopausal women aged 19 to 49 years. They were divided by 3 groups, overweight($23{\leqq}BMI<25$), obese($25{\leqq}BMI<30$) and morbidly obese($BMI{\geq}30$) according to their BMI. Body composition was determined by bioelectrical impedance analysis(BIA) method. Dietary intakes were examined by 3-day record method and nutrient intakes were analyzed by the Computer Aided Nutritional analysis program for professionals(CAN-pro 3.1). Serum lipid levels were measured using automatic biochemical analyzer(Selectra E). The average age, height weight and BMI of subjects were 31.7 years old, 159.3cm, 69.4kg, and $27.4kg/m^2$, respectively. Their average consumption of energy were 1712kca1 and 86.4% of estimated energy requirement(EER). Their mom ratio of carbohydrate:protein:fat was 55.2:14.6:30.2. Particularly, the fat consumption of subjects tended to be elevated with increasing BMI. There was no significant difference in nutrient intakes among 3 groups. But the mom intakes of Ca, Fe, vitamin C and Na of all the subjects were 70.1%, 81.2%, 75.7% of recommended intake(RI) and 258.9% of adequate intake(AI), respectively, The intakes of vegetables and seaweeds were significantly higher in morbidly obese group while the intake of fruits was higher in overweight group compared to the other groups. Serum lipid analysis showed that there were no significant differences in the levels of total cholesterol, triglyceride and LDL-cholesterol among the groups. But the serum HDL-cholesterol level of the overweight group was significantly higher than that of the other groups. LDL-/HDL-cholesterol ratio and AI index were significantly higher in the morbidly obese group compared to the other groups. In addition, The systolic blood pressure of morbidly obese group was significantly higher compared to the other groups. Overall data suggest that morbidly obese women have to more concern about reducing systolic blood pressure and nm lipid levels by decreasing their fat consumption and salt intakes as well as loosing body fat. In addition, all the subjects participated in this study have to be careful about their meals and health-related behaviors in order to prevent obesity-related chronic diseases.

Comparative Studies on the Fatty Acid Compositions of the Korean Salt Marsh Plants in the West Sea (한국서해에 서식하는 염생식물의 지방산 조성 비교)

  • Kim, You-Ah;Um, Young-Ran;Lee, Jung-Im;Kim, Hae-Jin;Lim, Sun-Young;Nam, Taek-Jeong;Seo, Young-Wan
    • KSBB Journal
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    • v.24 no.6
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    • pp.521-526
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    • 2009
  • Fatty acid is an important component of many biological processes. However, an imbalance in diet-especially, a n-3 polyunsaturated fatty acids (PUFAs) deficiency-causes several diseases such as diabetes, cardiovascular disease and cancer. In this study, we analyzed the fatty acid contents and compositions of 26 species of Korean salt marsh plants and found high fatty acid contents from S. herbacea ($148.75\;{\mu}g$/mg-dry wt.), S. komarvii ($119.05\;{\mu}g$/mg-dry wt.), C. heterocarpa ($79.23\;{\mu}g$/mg-dry wt.), A. capillaris ($71.65\;{\mu}g$/mg-dry wt.), and L. tetragonum ($67.02\;{\mu}g$/mg-dry wt.). In the case of saturated fatty acids (SFAs) composition, palmitic acid is richest in most salt marsh plants. On the other hand, oleic acid and linoleic acid are major components of monounsaturated fatty acid and n-6 PUFA, respectively. In addition, n-3 PUFAs such as LNA (linolenic acid), EPA (eicosapentaenoic acid), and DHA (docosahexaenoic acid) known as the main fatty acid components of fish oils and seaweeds, were also found in S. herbacea, S. komarvii, T. tetragonoides, A. capillaris and G. littoralis.

The Trend of Food and Nutrient Intakes of Korean(1969-1989) -The Second Report, Food Intake from the Annual Report of the National Nutrition Survey- (한국인의 식품 및 영양섭취상태 추이(1969-1989)-제 2보, 국민영양보고서에 의한 식품섭취상태을 중심으로-)

  • Park, Mi-A;Kim, Eul-Sang;Lee, Kyu-Han;Moon, Hyun-Kyung;Song, In-Jung;Tchai, Bum-Suk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.5
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    • pp.509-512
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    • 1992
  • With the annual report of National Nutrition survey, the trend of food intake of Korean is analyzed from 1969 to 1989. Total food intake is about 1,000g. The amount of plant foods intake is decreased but the amount of animal food intake is increased. Among plant foods, the consumption of cereals and grain product and starch and starch roots is decreased gradually. The degree of decreasing are evident in the amount of cereal and grain product intake, The amount of vegetable intake is kept fluctuating, but the amount of processed vegetable products intake shows an upward trend. The amount of legumes and their products, vegetables, fruits and seaweeds intake is increased each year. In addition, among animal foods, the amount of meat and products, eggs, fish and shellfish and milk and milk products intake is increased greatly.

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Studies on the Processing and Utilization of Seaweeds 1. Preparation of Powdered Sea Mustard, Undaria pinnatifida, Mixtures for Juice Type Beverage (해조류(海藻類)의 가공(加工) 및 이용(利用)에 관한 연구(硏究) 1. 미역분말쥬스제조(製造))

  • Lee, Eung Ho;Cha, Yong Jun;Kim, Jeong Gyun;Kwon, ChiI Sung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.4
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    • pp.382-386
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    • 1983
  • In order to utilize effectively sea mustard(Undaria pinnatifida) which is excellent in nutrition and medical action, powdered sea mustard juice was prepared and then its chemical composition and the stability of pigments were examined. Powdered sea mustard was made by washing fresh sea mustard with tap water to remove clay and sand, blanching at $85^{\circ}C$ for 20 sec in mixing solution(3% salt+1% $MgCO_3$), hot air drying($50-55^{\circ}C$, 3 hrs) after draining and pulverizing dried sea mustard to 200mesh. And then powdered sea mustard mixtures for juice type beverage was made by adding 0.75% of salt, 1.25% of sugar, 0.2% of ascorbic acid, 0.25% of flour of roasted barley and 0.8% of pulverized sea mustard to 100ml of water. Chemical composition of product was not scarcely changed during processing while amino-nitrogen content was increased and alginic acid and ash contents were decreased. The retention of chlorophyll and total carotenoid pigments of product against fresh sea mustard were 91.6% and 89.5% respectively. Judging from sensory evaluation, color, flavor, taste and dispersibility of powdered sea mustard juice were excellent and undesirable flavor of product was masked by addition of flour of roasted barley.

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