Polyphenol-rich fraction from Ecklonia cava (a brown alga) processing by-product reduces LPS-induced inflammation in vitro and in vivo in a zebrafish model

  • Kim, Seo-Young (Department of Marine Life Science, Jeju National University) ;
  • Kim, Eun-A (Department of Marine Life Science, Jeju National University) ;
  • Kang, Min-Cheol (Department of Marine Life Science, Jeju National University) ;
  • Lee, Ji-Hyeok (Department of Marine Life Science, Jeju National University) ;
  • Yang, Hye-Won (Department of Marine Life Science, Jeju National University) ;
  • Lee, Jung-Suck (Industry-Academic Cooperation Foundation, Jeju National University) ;
  • Lim, Tae Il (Taerim Trading Co., Ltd.) ;
  • Jeon, You-Jin (Department of Marine Life Science, Jeju National University)
  • Received : 2014.04.25
  • Accepted : 2014.06.03
  • Published : 2014.06.15


Ecklonia cava is a common edible brown algae that is plentiful in Jeju Island of Republic of Korea. Polyphenols from E. cava have strong anti-inflammatory activity. However, a large number of the by-products from E. cava processing are discarded. In the present study, to utilize these by-products, we assessed the anti-inflammatory activity of the polyphenol-rich fraction (PRF) from E. cava processing by-product (EPB) in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells. Four compounds, namely eckol, eckstolonol, dieckol, and phlorofucofuroeckol-A, were isolated and identified from PRF. We found that PRF suppressed the production of nitric oxide (NO), inducible nitric oxide synthase, and cyclooxygenase-2 in the LPS-induced cells. Furthermore, the protective effect of PRF was investigated in vivo in LPS-stimulated inflammation zebrafish model. PRF had a protective effect against LPS-stimulated toxicity in zebrafish embryos. In addition, PRF inhibited LPS-stimulated reactive oxygen species and NO generation. According to the results, PRF isolated from EPB could be used as a beneficial anti-inflammatory agent, instead of discard.



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