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Anti-inflammatory Effects of Enzymatic Extract from Ecklonia cava on TPA-induced Ear Skin Edema  

Ahn, Ginnae (Department of Marine Life Science, Cheju National University)
Park, Eun-Jin (College of Veterinary Medicine and Institute of Medical Science, Cheju National University)
Kim, Dae-Seung (Department of Marine Life Science, Cheju National University)
Jeon, You-Jin (Faculty of Marine Medicine Science, Cheju National University)
Shin, Tae-Kyun (College of Veterinary Medicine and Institute of Medical Science, Cheju National University)
Park, Jae-Woo (Department of Nuclear and Energy Engineering, Cheju National University)
Woo, Ho-Chun (College of Veterinary Medicine and Institute of Medical Science, Cheju National University)
Lee, Ki-Wan (Department of Marine Life Science, Cheju National University)
Jee, Young-Heun (College of Veterinary Medicine and Institute of Medical Science, Cheju National University)
Publication Information
Food Science and Biotechnology / v.17, no.4, 2008 , pp. 745-750 More about this Journal
Abstract
Anti-inflammatory potential of the enzymatic extract prepared by Kojizyme (ECK), a component of brown seaweeds Ecklonia cava (Alariaceae, Phaeophyta) in vivo was investigated. For the application of mouse ear edema model, 12-O-tetradecanoylphorbol acetate (TPA) was used, a topical inducer of a long-lasting inflammatory response. Our results demonstrated that ECK inhibited ear edema when topically applied to mouse ear skin. In histological evaluation, the inhibition activity of ECK on TPA-induced inflammation is similar to that of dexamethasone, although less strong. In addition, the mRNA expression levels of IL-$1{\beta}$, IFN-$\gamma$, TNF-$\alpha$, and cyclooxygenase-2 (COX2) and the immunoreactivity to inducible nitric oxide synthase (iNOS) and COX2 expressed mainly in inflammatory cells were down-regulated by ECK. These results indicate that ECK has anti-inflammatory effects through the inhibition of Th1 cytokines and 2 inducers of inflammation in TPA-induced ear skin edema.
Keywords
anti-inflammatory effect; Ecklonia cava; 12-O-tetradecanoylphorbol acetate (TPA); ear skin edema;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
Times Cited By SCOPUS : 3
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