• Title/Summary/Keyword: Sealing surface

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Solanum Nigrum Polysaccharide (SNL) Extract Effects in Transplanted Tumor-bearing Mice - Erythrocyte Membrane Fluidity and Blocking of Functions

  • Yuan, Hong-Liang;Liu, Xiao-Lei;Liu, Ying-Jie
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.23
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    • pp.10469-10473
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    • 2015
  • Background: Solanum nigrum L. has been used in traditional Chinese medicine because of its diuretic and antipyretic effects. The present research concerned effects of crude polysaccharides isolated from Solanum nigrum L. on erythrocyte membranes of tumor-bearing $S_{180}$ and $H_{22}$ in mice. Materials and Methods: Fluorescence-labeled red blood cell membranes were used with DPH fluorescence spectrophotometry to examine erythrocyte membrane fluidity, and colorimetry to determine degree of erythrocyte surface membrane blocking. Extent of reaction by tumor-bearing mice with the enzyme erythrocyte membrane bubble shadow detection of red cell membrane variation in the degree of closure before and after administration. Results: Solanum nigrum polysaccharide could significantly improve the $S_{180}$ and $H_{22}$ tumor-bearing mice erythrocyte membrane fluidity, compared with the control group, the difference was significant (p<0.01), SNL can significantly improve the red blood cell membrane and then $S_{180}$ tumor-bearing mice sealing ability, compared with the negative control group, the difference was significant(p<0.05, p<0.01). $H_{22}$ tumor-bearing mice can increase red cell membrane and then sealing ability, the difference was significant (p<0.05). Solanum nigrum polysaccharide degree of fluidity and blocking two transplanted tumors in mice restored the ability to raise the red cell membrane has a significant effect. Conclusions: Solanum nigrum L.-type mice transplanted tumor can affect the red blood cell membrane fluidity and re-closed, through the red cell membrane of red blood cells to enhance the immune function of the possibility of erythrocyte immunity against tumor formation garland provide experimental basis.

Characterization of Three Antifungal Calcite-Forming Bacteria, Arthrobacter nicotianae KNUC2100, Bacillus thuringiensis KNUC2103, and Stenotrophomonas maltophilia KNUC2106, Derived from the Korean Islands, Dokdo and Their Application on Mortar

  • Park, Jong-Myong;Park, Sung-Jin;Ghim, Sa-Youl
    • Journal of Microbiology and Biotechnology
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    • v.23 no.9
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    • pp.1269-1278
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    • 2013
  • Crack remediation on the surface of cement mortar using microbiological calcium carbonate ($CaCO_3$) precipitation (MICP) has been investigated as a microbial sealing agent on construction materials. However, MICP research has never acknowledged the antifungal properties of calcite-forming bacteria (CFB). Since fungal colonization on concrete surfaces can trigger biodeterioration processes, fungi on concrete buildings have to be prevented. Therefore, to develop a microbial sealing agent that has antifungal properties to remediate cement cracks without deteriorative fungal colonization, we introduced an antifungal CFB isolated from oceanic islands (Dokdo islands, territory of South Korea, located at the edge of the East Sea in Korea.). The isolation of CFB was done using B4 or urea-$CaCl_2$ media. Furthermore, antifungal assays were done using the pairing culture and disk diffusion methods. Five isolated CFB showed $CaCO_3$ precipitation and antifungal activities against deteriorative fungal strains. Subsequently, five candidate bacteria were identified using 16S rDNA sequence analysis. Crack remediation, fungi growth inhibition, and water permeability reduction of antifungal CFB-treated cement surfaces were tested. All antifungal CFB showed crack remediation abilities, but only three strains (KNUC2100, 2103, and 2106) reduced the water permeability. Furthermore, these three strains showed fungi growth inhibition. This paper is the first application research of CFB that have antifungal activity, for an eco-friendly improvement of construction materials.

THE PHYSICAL PROPERTIES AND HEALING EFFECT OF CALCIUM SULFATE-HYDROXYAPATITE COMPOUND ON ROOT PERFORATION (Calcium sulfate-Hydroxyapatite 혼합재의 물성 및 치근천공 치유효과에 관한 연구)

  • Lee, Seung-Jong;Kim, Kyoung-Nam
    • Restorative Dentistry and Endodontics
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    • v.22 no.2
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    • pp.739-750
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    • 1997
  • Treatment of root perforation elicits special considerations due to its blood-contaminated circumstances. It is known that conventional dental restorative materials are all leaking. Calcium sulfate is the material which react with water to become chemically set. This study, therefore, was performed to develop a new compound containing calcium sulfate and to evaluate its physical and biological characteristics. Three materials were used, IRM, calcium sulfate, calcium sulfate-hydroxyapatite compound. The composition of the calcium sulfate-hydroxyapatite compound was basically 50 % of calcium sulfate and 50 % of hydroxyapatite mixed with guajacol. The materials were mixed in conventional way and underwent four physical test procedures, setting time, solubility test, compressive strength, and marginal leakage test. All materials were evaluated under the scanning electron microscope to examine the marginal sealing ability. Animal experiment was also performed to test the materials' tissue response. Twenty-four dog's premolars were tested with either furcation perforations or apical retro-fillings. From the results, we found that calcium sulfate possess the good marginal sealing ability. However, calcium sulfate creates many voids which is caused by crystal thrusting action when it reacts with water. It seemed that the voids caused disintegration of the material which eventually lead to tissue reaction. By compounding calcium sulfate and hydroxyapatite, we were able to obtain the better physical properties but it showed larger marginal gap between the material and the root surface. Within the six weeks observation period, both IRM and calcium sulfate-hydroxyapatite compound showed good tissue responses in animal experiment. It is concluded that calcium sulfate would be the material of choice in root perforation repair, but the physical property needs to be further improved.

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THE MARGINAL SEALING EFFECT OF COMPOSITE RESIN INLAYS ACCORDING TO THE LUTING TECHNIQUES (합착방법(合着方法)에 따른 복합(複合)레진 인레이의 변연폐쇄효과(邊緣閉鎖效果))

  • Moon, Young-Deok;Cho, Kyeu-Zeung
    • Restorative Dentistry and Endodontics
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    • v.16 no.1
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    • pp.121-132
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    • 1991
  • The purpose of the study was to evaluate the marginal sealing effect of composite resin inlays according to the luting techniques and compare them to the conventional direct resin filling technique. 90 cavities of class V were prepared on the buccal surface of 90 extracted molar teeth, which were divided into four groups. Cavities of control group were directly filled with Scotchbond 2 and P - 50, and those of composite resin inlay groups were luted with one of the followings: Adhesive bond followed by Adhesive cement, All bond followed by Adhesive cement, Fuji - ionomer type L All the specimens were immersed in India ink dye solution for 7 days at $37^{\circ}C$ incubator after thermocycling between $5^{\circ}C$ and $60^{\circ}C$ and longitudinally sectioned with diamond disk inot two parts All the specimens were observed at the occlusal and gingival margins and statistical analysis was performed. The results were as follows: 1. Groups filled with composite resin inlay showed less marginal leakage than the group directly filled(p<0.01). 2. There was no significant difference in marginal leakage between composite resin inlay groups luted with Adhesive bond followed by Adhesive cement and the group luted with All bond followed by Adhesive cement(p>0.05). 3. At occlusal margins, Composite resin inlay group luted with Adhesive bond followed by Adhesive cement showed less marginal leakage than the group luted with Fuii ionomer type I(p<0.01). At gingival margins, composite resin inlay group luted with All bond followed by Adhesive cement showed less marignal leakage than the group luted with Fuji ionomer type I(P<0.01).

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Anti-washout Grouts for Underwater Sealing of Karst Cavities and Construction Research Tendencies (수중 불분리성 그라우트 개발 기술 동향)

  • Baluch, Khaqan;Kim, Jung-Gyu;Kim, Jong-Gwan;Yu, Ji-Yun;Yang, Hyung-Sik
    • Explosives and Blasting
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    • v.38 no.4
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    • pp.46-52
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    • 2020
  • Although anti-washout grouts are used extensively in underwater targets, major constraints continue to be associated with their use. These include poor bonding strength, poor pumpability, and loss of high strength in everyday engineering applications. In this study, based on the literature pertaining to self-compacted, non-dispersive, anti-washout grouts, a review of research trends in anti-washout grouts for underwater construction and sealing of karst cavities was carried out in order to determine the problems faced in this field. Grouts used under water suffer a loss of strength and bonding strength in comparison to grouts cast in air. Researchers are designing high-viscosity grouts to overcome the inrush of water and seal karst cavities; however, in doing so, they have inadvertently caused serious problems pertaining to the pumpability of these grouts and concretes in deep target locations. Thus, the majority of the anti-washout grouts and concretes that have been developed are not applicable to deep target environments, instead being suitable for only near-surface targets.

Geophysical and mechanical investigation of different environmental effects on a red-bed soft rock dam foundation

  • Liming Zhou;Yujie Li;Fagang Wang;Yang Liu
    • Geomechanics and Engineering
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    • v.34 no.2
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    • pp.139-154
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    • 2023
  • Red-bed soft rock is a common stratum and it is necessary to evaluate the mechanical properties and bearing capacity of red-bed soft rock mass affected by different environmental effects. This paper presents a complete procedure for evaluating the bearing capacity of red-bed soft rock by means of geophysical exploration and in-situ rock mechanics tests. Firstly, the thickness of surface loosened rock mass of red-bed soft rock was determined using geophysical prospecting method. Then, three environmental effects, including natural weathering effect, dry-wet cycling effect and concrete sealing effect, were considered. After each effect lasted for three months, in-situ rock mass mechanical tests were conducted. The test results show that the mechanical properties of rock mass considering the sealing effect of concrete were maintained. After considering the natural weathering effect, the mechanical parameters decrease to a certain extent. After considering the effect of dry-wet cycling, the decreases of mechanical parameters are the most significant. The test results confirm that the red-bed soft rock dam foundation rock mass will be significantly affected by various environmental effects. Therefore, combined with the mechanical test results, some useful implementations are proposed for the construction of a red-bed soft rock dam foundation.

Evaluation of Adhesion Characteristics of Crack Sealants Used in Asphalt Concrete Pavement (아스팔트 콘크리트 포장용 균열실링재의 부착특성 평가)

  • Lee, Jae-Jun;Kim, Seung-Hoon;Baek, Jong-Eun;Lim, Jae-Kyu;Kim, Yong-Joo
    • International Journal of Highway Engineering
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    • v.17 no.2
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    • pp.55-62
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    • 2015
  • Cracking is an inevitable fact of asphalt concrete pavements and plays a major role in pavement deterioration. Pavement cracking is one of the main factors determining the frequency and method of repair. Cracks can be treated with a number of preventative maintenance actions, including overlay surface treatments such as slurry sealing, crack sealing, or crack filling. Pavement cracks can show up as one or all of the following types: transverse, longitudinal, fatigue, block, reflective, edge, and slippage. Crack sealing is a frequently used pavement maintenance treatment because it significantly extends the pavement service life. However, crack sealant often fails prematurely due to a loss of adhesion. Because current test methods are mostly empirical and only provide a qualitative measure of the bond strength, they cannot accurately predict the adhesive failure of the sealant. This study introduces a laboratory test aimed at assessing the bonding of hot-poured crack sealant to the walls of pavement cracks. A pneumatic adhesion tensile testing instrument (PATTI) was adopted to measure the bonding strength of the hot-poured crack sealant as a function of the curing time and temperature. Based on a limited number of test results, the hot-poured crack sealants have very different bonding performances. Therefore, this test method can be proposed as part of a newly developed performance-based standard specification for hot-poured crack sealants for use in the future. PURPOSES : The purpose of this study was to evaluate both the adhesion and failure performance of a crack sealant as a function of its curing time and curing temperature. METHODS: A pneumatic adhesion tensile testing instrument (PATTI) was adopted to measure the adhesion performance of a crack sealant as a function of the curing time and curing temperature. RESULTS: With changes in the curing time, curing temperature, and sealant type, the bond strengths were found to be significantly different. Also, higher bond strengths were measured at lower temperatures. Different sealant types produced completely different bond strengths and failure behaviors. CONCLUSIONS: The bonding strength of an evaluated crack sealant was shown to differ depending on various factors. Two sealant types, which were composed of different raw materials, were shown to perform differently. The newly proposed test offers the possibility of evaluating and differentiating between different crack sealants. Based on alimited number of test results, this test method can be proposed as part of a newly developed performance-based standard specification for crack sealants or as part of a guideline for the selection of hot-poured crack sealant in the future.

COMPARISON OF APICAL SEALING EFFICACIES USING DIFFERENT PLUGGING DEPTH IN CONTINUOUS WAVE OF OBTURATION TECHNIQUE (Continuous wave of obturation technique에서 플러거의 다양한 적용 깊이에 따른 근단부 폐쇄효율 비교)

  • Lee, Sang-Jin;Park, Jeong-Kil;Hur, Bock;Kim, Hyeon-Cheol
    • Restorative Dentistry and Endodontics
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    • v.32 no.6
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    • pp.491-497
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    • 2007
  • The purpose of this study was to compare apical sealing ability of continuous wave canal filling technique according to various heat source plugging depths. Eighty one extracted human premolars with straight root were cleaned and shaped to size 35 using .06 taper rotary NiTi file. After cleansing and shaping, the teeth were divided into 5 groups following the heat source probing depths from the apex; 3, 4, 5, 6 and 7 mm. All specimens were filled using E&Q plus with #35/.06 tapered gutta-percha cone. The positive control teeth were not filled. All teeth were coated with nail varnish except the apical 1 mm around the apical foramen. Negative control teeth were completely sealed include the apical foramen. All specimens were immersed in 1% methylene blue solution for 72 hours. Then the specimens were sectioned horizontally at 1, 2 and 3 mm from the root apex. Each sectioned surface was photographed using a digital camera attached to the stereomicroscope at $12.5{\times}2.5$ fold magnification. All points at 1, 2 and 3 mm were summed as final score of one specimen. Statistical analysis of the collected data was performed. Under the condition of this study. there was no significant difference between the heat source plugging depths of 3, 4, 5, 6 and 7 mm in apical sealing ability. All of apical heat source plugging depth from 3 to 7 mm including Buchanan's protocol -from 5 to 7 mm- seems to be acceptable in clinical application.

A Study on the Lubrication Characteristics of the Hydro-Static Journal Bearing for a Crank Shaft Pin Turner by Applying Dimple (딤플이 크랭크 샤프트 핀 터너 유정압 베어링의 윤활특성에 미치는 영향에 관한 연구)

  • Seo, Mi Na;Lee, Deug Woo;Ha, Yang Hyup;Lee, Sang Min;Kim, Yong Woo
    • Journal of the Korean Society for Precision Engineering
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    • v.31 no.8
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    • pp.715-720
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    • 2014
  • Recently, surface texturing technologies have been widely used in lots of industries to increase the machinery efficiency. In this research, the lubrication characteristics of a crank shaft pin turner bearing with dimples were studied. When increasing the dimples, the load carrying capacity due to the increased pressure was increased because those have sealing effects. Also, the run-out error of the bearing was decreased. Therefore, it is important to consider the depth, the number and the distribution of dimples when designing the hydrostatic journal bearing.

Sealing of leakage on the raw surface of pulmonary resection with histoacryl in cats (고양이의 절제된 폐단면에 histoacryl 의 폐쇄효과에 대한 실험적 연구)

  • Lee, Du-Yeon;O, Jung-Hwan;Bang, Jeong-Hyeon
    • Journal of Chest Surgery
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    • v.23 no.2
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    • pp.231-235
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    • 1990
  • Segmental resection or wedge resection of the lung and direct cutting across of bronchi frequently results in postoperative airleaks or bronchopleural fistula in some cases. Many methods have been devised to handle air leak problems by oversuturing the raw edges, the application of pleural onlay flaps, the use of cautery or tissue adhesives, but these still has not solved the problem of air leaking from raw surfaces of the lung with only partial successful we have tried the use of histoacryl to closure the raw surface of the resected lung and compared it’s results with of the closure with chromic catgut sutures in cats from May 1989 to Jan. 1990 at the department of the thoracic and cardiovascular surgery, Yonsei University, College of Medicine. Ninety lobe of the lungs were used in this study and forty nine of which have been divided segmentally and closed with histoacryl on the raw surfaces. Forty one of which have been divided segmentally and closed with sutures using chromic catgut for control. There were air leakages in twelve cases among 49 cases with applying histoacryl and airleak were in two cases among 12 cases with reapply histoacryl, But there were air leak in two cases among 41 cases with sutures using chromic catgut and airleak were in one case among with resutures using chromic catgut.

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