• Korean Journal of Ichthyology
• /
• v.33 no.2
• /
• pp.142-147
• /
• 2021

A single specimen (52.5 mm SL) of Neoclinus chihiroe, belonging to the family Chaenopsidae, was firstly collected from Dokdo, East Sea of Korea on 9 December 2020. This species was characterized by a black spot between the first and the second dorsal spines, and a black spot on upper part of opercular membrane. The species was morphologically similar to N. okazakii, but distinctly distinguished by pectoral fin base without a black dot. This study documents the first record of N. chihiroe in Korean waters and suggests the new Korean name 'Dong-hae-bi-neul-be-do-la-chi' for the species.

• ALGAE
• /
• v.34 no.2
• /
• pp.127-140
• /
• 2019

The species in the dinoflagellate order Suessiales have 5-24 latitudinal paraplate series and include many fossil and extant species. There have been a few studies on the ecophysiology of the phototrophic species Biecheleriopsis adriatica, and no study on its predators. Thus, we explored the feeding occurrence by common heterotrophic protists on B. adriatica and the growth and ingestion rates of the heterotrophic dinoflagellate Oxyrrhis marina on B. adriatica BATY06 as a function of prey concentration. The common heterotrophic dinoflagellates Aduncodinium glandula, O. marina, Gyrodinium dominans, Gyrodinium moestrupii, Luciella masanensis, Pfiesteria piscicida, and Oblea rotunda and two naked ciliates Strombidinopsis sp. and Pelagostrobilidium sp. were able to feed on B. adriatica, but the heterotrophic dinoflagellate Polykrikos kofoidii was not. However, B. adriatica supported the positive growth of O. marina, but did not support that of G. dominans and O. rotunda. With increasing prey concentrations, the growth and ingestion rates of O. marina on B. adriatica increased and became saturated. The maximum growth rate of O. marina on B. adriatica was $0.162d^{-1}$. Furthermore, the maximum ingestion rate of O. marina on B. adriatica was $0.2ng\;C\;predator^{-1}\;d^{-1}$ ($2.0cells\;predator^{-1}\;d^{-1}$). In the order Suessiales, the feeding occurrence by common heterotrophic protists on B. adriatica is similar to that on Effrenium voratum and Biecheleria cincta, but different from that on Yihiella yeosuensis. However, the growth and ingestion rates of O. marina on B. adriatica are considerably lower than those on E. voratum and B. cincta, but higher than those on Y. yeosuensis. Therefore, B. adriatica may be less preferred prey for O. marina than E. voratum and B. cincta, but more preferred prey than Y. yeosuensis.

• Journal of Ginseng Research
• /
• v.43 no.2
• /
• pp.218-225
• /
• 2019

Background: Streptococcus pneumoniae, more than 90 serotypes of which exist, is recognized as an etiologic agent of pneumonia, meningitis, and sepsis associated with significant morbidity and mortality worldwide. Immunization with a pneumococcal pep27 mutant (${{\Delta}}pep27$) has been shown to confer comprehensive, long-term protection against even nontypeable strains. However, ${{\Delta}}pep27$ is effective as a vaccine only after at least three rounds of immunization. Therefore, treatments capable of enhancing the efficiency of ${{\Delta}}pep27$ immunization should be identified without delay. Panax ginseng Mayer has already been shown to have pharmacological and antioxidant effects. Here, the ability of Korean Red Ginseng (KRG) to enhance the efficacy of ${{\Delta}}pep27$ immunization was investigated. Methods: Mice were treated with KRG and immunized with ${{\Delta}}pep27$ before infection with the pathogenic S. pneumoniae strain D39. Total reactive oxygen species production was measured using lung homogenates, and inducible nitric oxide (NO) synthase and antiapoptotic protein expression was determined by immunoblotting. The phagocytic activity of peritoneal macrophages was also tested after KRG treatment. Results: Compared with the other treatments, KRG significantly increased survival rate after lethal challenge and resulted in faster bacterial clearance via increased phagocytosis. Moreover, KRG enhanced ${{\Delta}}pep27$ vaccine efficacy by inhibiting reactive oxygen species production, reducing extracellular signal-regulated kinase apoptosis signaling and inflammation. Conclusion: Taken together, our results suggest that KRG reduces the time required for immunization with the ${{\Delta}}pep27$ vaccine by enhancing its efficacy.

• ALGAE
• /
• v.36 no.4
• /
• pp.263-283
• /
• 2021

To explore the ecophysiological characteristics of the kleptoplastidic dinoflagellate Shimiella gracilenta, we determined its spatiotemporal distribution in Korean coastal waters and growth and ingestion rates as a function of prey concentration. The abundance of S. gracilenta at 28 stations from 2015 to 2018 was measured using quantitative real-time polymerase chain reaction. Cells of S. gracilenta were detected at least once at all the stations and in each season, when temperature and salinity were 1.7-26.4℃ and 9.9-35.6, respectively. Moreover, among the 28 potential prey species tested, S. gracilenta SGJH1904 fed on diverse prey taxa. However, the highest abundance of S. gracilenta was only 3 cells mL^-1 during the study period. The threshold Teleaulax amphioxeia concentration for S. gracilenta growth was 5,618 cells mL^-1, which was much higher than the highest abundance of T. amphioxeia (667 cells mL^-1). Thus, T. amphioxeia was not likely to support the growth of S. gracilenta in the field during the study period. However, the maximum specific growth and ingestion rates of S. gracilenta on T. amphioxeia, the optimal prey species, were 1.36 d^-1 and 0.04 ng C predator^-1 d^-1, respectively. Thus, if the abundance of T. amphioxeia was much higher than 5,618 cells mL^-1, the abundance of S. gracilenta could be much higher than the highest abundance observed in this study. Eurythermal and euryhaline characteristics of S. gracilenta and its ability to feed on diverse prey species and conduct kleptoplastidy are likely to be responsible for its common spatiotemporal distribution.

• Journal of the Korean Wood Science and Technology
• /
• v.32 no.1
• /
• pp.88-95
• /
• 2004

The purpose of this study is to identify the species of wood members used in the Keunjungjeon Hall, main building of the Kyungbok palace in Seoul. The total 144 samples consist of 52 from pillars and 92 from other wood members. Only two species were identified, which were Abies holophylla Max. (needle fir) and Pinus densiflora Sieb. et Zucc. (Japanese red pine). For the pillars, A. holophylla were more abundantly used than P. densiflora. Among 20 outer pillars ('Pyeongju'), 11 were A. holophylla, and 9 were P. densiflora. Among 12 inner pillars ('Naejinkoju'), 7 were A. holophylla, and 5 were P. densiflora. Among 4 inner corner pillars ('Gwikoju'), 3 were A. holophylla and 1 was P. densiflora. For 92 other wood members, only 2 purlins were A. holophylla and the others were all P. densiflora. The results suggest that the common opinion 'Palace buildings of Korea are made from red pine woods' should be corrected. We think that fir logs might be used for the pillars instead of pines because long and straight pines were not available during 1860s due to heavy utilization of pines as construction and fuel materials in the late Chosun period.

• Korean Journal of Ichthyology
• /
• v.34 no.4
• /
• pp.288-293
• /
• 2022

A single specimen of Neoclinus nudiceps, belongs to the family Chaenopsidae, was first collected from Hongdo Island, Yellow Sea of Korea on June 13, 2012. This species is characterized by a lateral line reaching to below the 3rd~4th spines of the dorsal fin, two pairs of supraorbital cirri arranged in one row, anterior supraorbital cirri with six tips, and posterior supraorbital cirri with one tip. The species is morphologically similar to N. monogrammus, but differs in the length of lateral line (reaching 3rd~4th dorsal spines in N. nudiceps vs. reaching 6th~9th dorsal spines in N. monogrammus, respectively). This study documents the first record of N. nudiceps in Korean waters and suggests the new Korean name "Jjalb-eun-teol-bi-neul-be-do-la-chi" for the species.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2020.08a
• /
• pp.27-27
• /
• 2020

Daphne L. (Thymelaceae) comprises about 95 species distributing worldwide from N Africa, N India, SE Asia to E Asia and the coast of the Mediterranean of Europe. In Korea, five species of this genus have been described. In this study, we included four species (D. genkwa, D. pseudomezereum, D. kiusiana, D. jejudoensis) from Korea, excluding cultivated D. odora. The morphological characters through local surveys and the re-classification of the specimens collected in the Korea National Herbarium (KH) were carried out and distribution maps for each taxon were also prepared. The major characters include habit, trichomes in winter bud, leaf, and twig, phyllotaxis, inflorescence, size of calyx lobe and trichomes in the calyx tube, etc. The distribution map showed that D. genkwa is mainly distributed in the coastal area of Hwanghaenam-do, Pyeongannam-do, Jeollabuk-do and Jeollanam-do, whereas D. pseudomezereum is distributed in the limestone zone of Gangwon-do, Jeollabuk-do, and Gyeongsangbuk-do. Similarly, D. kiusiana is mostly found in Jeollanam-do, Gyeongsangnam-do, and Jeju-do. In addition, D. jejudoensis is known to be distributed in forests of Murueng, Andeok, and Seonheul-ri in Jeju-do, but recently, new habitat is discovered in the island forest areas of Jeollanam-do. However, some of these individuals showed the characteristics of D. kiusiana, thus before come to any conclusion detailed taxonomic review of D. jejudoensis and D. kiusiana is required.

• Journal of Microbiology and Biotechnology
• /
• v.33 no.11
• /
• pp.1448-1456
• /
• 2023

A Gram-positive, non-motile, and non-spore-forming lactic acid bacterium, designated as BK2^T, was isolated from kimchi, a Korean traditional fermented vegetable food, and the taxonomic characteristics of strain BK2^T, along with strain LMG 11983, were analyzed. Both strains optimally grew at 30℃, pH 7.0, and 1.0% NaCl. Cells of both strains were heterofermentative and facultatively anaerobic rods, demonstrating negative reactions for catalase and oxidase. Major fatty acids (>10%) identified in both strains were C_18:1 ω9c, C_16:0, and summed feature 7 (comprising C_19:1 ω6c and/or C_19:1 ω7c). The genomic DNA G+C contents of both strains were 44.7 mol%. The 16S rRNA gene sequence similarity (99.9%), average nucleotide identity (ANI; 99.9%), and digital DNA-DNA hybridization (dDDH; 99.7%) value between strains BK2^T and LMG 11983 indicated that they are different strains of the same species. Strain BK2^T was most closely related to Weissella confusa JCM 1093^T and Weissella cibaria LMG 17699^T, with 100% and 99.4% 16S rRNA gene sequence similarities, respectively. However, based on the ANI and dDDH values (92.3% and 48.1% with W. confusa, and 78.4% and 23.5% with W. cibaria), it was evident that strain BK2^T represents a distinct species separate from W. confusa and W. cibaria. Based on phylogenetic, phenotypic, and chemotaxonomic features, strains BK2^T and LMG 11983 represent a novel species of the genus Weissella, for which the name Weissella fermenti sp. nov. is proposed. The type of strain is BK2^T (=KACC 22833^T=JCM 35750^T).

• Ocean and Polar Research
• /
• v.33 no.spc3
• /
• pp.349-358
• /
• 2011

In order to ascertain latitudinal variation of lipid contents and compositions in copepods, we collected warm water copepod species (Euchaeta sp. and Pleuromamma spp.) from four different regions from low (sub-tropical) to mid (temperate) latitudes in the Northwest Pacific Ocean. Total lipid contents of Pleuromamma spp. were about 11 $ug{\cdot}ind^{-1}$ with little latitudinal variation, whereas Euchaeta sp. showed slightly higher lipid content (20 $ug{\cdot}ind^{-1}$) than Pleuromamma spp. with latitudinal gradient (low at subtropic and high at temperate). Wax esters, known as the major storage lipid classes, were found to be the dominant lipid classes (accounting for more than 35% of total lipids) in Euchaeta sp., whereas in Pleuromamma spp., phospholipids, known as cellular membrane components, were the dominant lipid classes. However, the exception was specimens from warm pool region exhibiting dominance in storage of lipids as a form of triacylglycerols. Among fatty acids, polyunsaturated fatty acids (PUFA), especially docosahexaenoic acid (DHA : 22:6(n-3)) (about 35% of total fatty acids), were most abundant in Euchaeta sp., while saturated fatty acids (SAFA), specially hexadecanoic acid (16:0) (about 30% of total fatty acids), were most abundant in Pleuromamma spp.. Among the neutral fraction of lipids, phytol, originated from the side chain of chlorophyll, was found in all samples which generally indicate active copepods feeding on algae. While only trace amounts of short-chain fatty alcohols were found in Pleuromamma spp., significant amounts of fatty alcohols were found in Euchaeta sp.. Particularly, significant amounts of long chain monounsaturated fatty alcohols (20:1 and 22:1), generally found in cold water species, were found in Euchaeta sp. from low latitudes. The latitudinal variation of trophic lipid markers in these copepods could be significantly related with in-situ food availability and species-specific diet preference. The result of this study suggests that the lipid contents and compositions in copepods may not only indicate their nutritional condition and feeding ecology but also provide insight into species-specific living strategies under different environmental conditions (i.e. water temperature, food availability).

• Korean Journal of Ecology and Environment
• /
• v.46 no.2
• /
• pp.241-250
• /
• 2013

Developmental process of vegetation over years after afforestation was analyzed in the Japanese larch (Larix kaempferi) plantations with different stand ages. The height and diameter of Japanese larch increased rapidly until 24 years after afforestation and tended to be blunted thereafter. The density of Japanese larch was similar with each other in the 8 and 17 years old stands but was reduced greatly in the 24 years old plantation and changing little thereafter. The floristic composition of the Japanese larch plantation did not show any clear trend depending on stand ages. The differences of species composition among plantation themselves with different ages were bigger than those between plantations and the reference stands, Mongolian oak (Quercus mongolica) stands. Japanese larch plantations showed a trend of succession to native vegetation dominated by Mongolian oak based on the results of analysis of frequency distribution by diameter class of major tree species. Species richness and diversity of all plantations were higher than those of the reference vegetation. As were shown in the above mentioned results, it can be evaluated that Japanese larch plantations practiced in the level of the functional restoration achieved successful restoration based on the floristic composition similar to the reference vegetation, the successional trend toward native vegetation, and higher species diversity compared with the reference vegetation.