• Korean Journal of Medicinal Crop Science
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• v.17 no.6
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• pp.388-396
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• 2009

The present study investigated an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcuma longae radix, Phellinus linteus, and Scutellariae radix for possible neuroprotective effects on neurotoxicity induced by amyloid $\beta$ protein ($A{\beta}$) (25-35) in cultured rat cortical neurons and antidementia activity in mice. Exposure of cultured cortical neurons to $10\;{\mu}M$ $A{\beta}$ (25-35) for 36 h induced neuronal apoptotic death. At $1-50\;{\mu}g/m{\ell}$, HS0608 inhibited neuronal death, elevation of intracellular calcium concentration ($[Ca^{2+}]_i$), and generation of reactive oxygen species (ROS) induced by $A{\beta}$ (25-35) in primary cultures of rat cortical neurons. Memory loss induced by intracerebroventricular injection of ICR mice with 15 nmol $A{\beta}$ (25-35) was inhibited by chronic treatment with HS0608 (25, 50 and 100 mg/kg, p.o. for 7 days) as measured by a passive avoidance test. From these results, we suggest that the antidementia effect of HS0608 is due to its neuroprotective effect against $A{\beta}$ (25-35)-induced neurotoxicity and that HS0608 may have a therapeutic role in preventing the progression of Alzheimer's disease.

• The Journal of Korean Obstetrics and Gynecology
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• v.34 no.3
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• pp.1-14
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• 2021

Methods: We investigated the ability to induce decidualization of human endometrial stromal cells and invasive ability of human trophoblast cells by water extract of three types herbal medicines (Dangguijakyak-san, Siryung-tang, Antae-eum) and ethanol extract on Scutellariae Radix. Results: In the process of decidualization of endometrial stromal cells, three herbal medicines including Dangguijakyak-san, Siryung-tang, and Scutellariae Radix increased the production of decidual markers such as prolactin (PRL) and insulin-like growth factor-binding protein 1 (IGFBP1). However, Antae-eum increased mRNA levels of PRL and IGFBP and secretion of IGFBP in decidual stromal cells, but not PRL. Four herbal medicines inhibited the invasion of trophoblast cells. Conclusions: Four herbal medicines may play a role in implantation in women with reproductive failures. However, further studies are warranted to elucidate whether these medications are helpful in the maintenance of pregnancy.

• Journal of Haehwa Medicine
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• v.14 no.1
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• pp.35-42
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• 2005

Objective : The purpose of this research is to examine the effects of Scutellariae Radix(SR) extract on immune cells in ovalbumin(OVA)-induced asthmatic mice. Methods : In vitro, C57BL/6 mice were exposed to OVA for 6 weeks. The mice lungs were taken out, chopped, grinded with collagenase and activated by rIL-3/rIL-5. The lung cells were treated with SR extract and analyzed by a flow cytometer. Results : The population of granulocytes/lymphocytes, CD3e-/CCR3+, CD69+/CD3e+, CD23+/B220+, CD4+ cells in the SR group in vitro decreased and population of CD8+ cells in the 100 and $10{\mu}g/m{\ell}$ SR group in vitro decreased, compared with that of control group. Conclusion : The results of this study support a role for SR as an effective treatment for asthma in its experimental success in significantly decreasing inflammation and asthma reactions.

• Journal of Nutrition and Health
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• v.37 no.6
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• pp.431-439
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• 2004

Scutellariae Radix (Scu.), one of the immune-regulatory substances, is recognized to play the role in the metabolic process of inflammation, allergy and immunity. It has been traditionally used in the Oriental medicine to treat inflammatory bowel diseases (IBD). The purpose of this study was to evaluate the effects of water extracts of Scutellariae Radix on the spleen lymphocyte immune function in the Balb/c female mice treated with dextran sodium sulfate (DSS) to induce colitis. Water extract of Scutellariae Radix (100 mg/kg) and sulfasalazine (50 mg/kg) were administrated orally for 2 weeks of experimental period. Mice were divided into three experimental groups randomly: DSS group (5％ DSS was ad libitum for 5 days) as control group, DSS ＋ Scu. (water extracts of Scutellariae Radix for 2 weeks after 5％ DSS was ad libitum for 5 days) as experimental group, and DSS ＋ Sulfasalazine group (Sulfasalazine for 2 weeks after 5％ DSS was ad libitum for 5 days) as positive control group. Levels of Ig A, Ig E, CD4$^{＋}$, CD8$^{＋}$, TNF-$\alpha$ and other cytokines were measured. Treatment of DSS for 5 days induced bowel inflammation and the treatment with Scu. water exteract and sulfasalazine significantly recovered the damage. The length of intestine of DSS group was significantly shorter than that of other groups. The serum and fecal concentration of Ig A of SS ＋ Scu group was higher than those of DSS group. The contents of CD4$^{＋}$ T cells was higher in the DSS ＋ Scu. group than the other groups and CD8$^{＋}$ T cells was the lowest in DSS ＋ Sulfasalazine group. The Ig A level of cultured supernatant of spleen lymphocyte was the highest, while the Ig E level was the lowest in SS ＋ Scu group. The concentration of TNF-$\alpha$, cytokine secreted from the Th1 cell in the supernatant spleen lymphocyte, was the highest in the DSS group and the lowest in the DSS ＋ Scu. group. The concentration of IFN-${\gamma}$ and ll...-12 was lower in the DSS ＋ Scu. group than those of the other groups. The concentration of IL-4 in the supernatant of spleen lymphocyte was the lowest in the DSS ＋ Scu. group but IL-10 was not significantly different. Based on these findings, water extract of Scutellariae Radix exhibited the inhibitory effect via IL-4 production thereby inhibited the production of Ig E and strengthened immune system, and alleviated injury in DSS- induced colitis mice model.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.5
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• pp.1344-1348
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• 2008

Anti-microbial activities of Scutellariae Radix Extracts and Sophora flavecens Extracts was tested by formulation, such as skinsoftener, emulsion for 4 weeks. Control contained no preservative, test group contained 1.00% Scutellariae Radix extracts and 1.00% Sophora flavecens extracts and positive control contained parabens and imidazolidinyl urea. To determine the anti-microbial activity of these extract, the 4 germs such as Escherichia coil, Pseudomonas aeruginosa, Staphylococcus aures, Canida albicans were used. The test groups showed significant anti-microbial activities against the 4 germs at 2 and 3 weeks as compared with control. Anti-microbial activities of these extracts were similar to positive control. Considering that the Scutellariae Radix Extracts and the Sophora flavecens Extracts have a significant anti-microbial activities against 4 germs, it is possible as natural preservative in cosmetics.

• Journal of dental hygiene science
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• v.2 no.1
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• pp.31-38
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• 2002

This study was to investigate the antibacterial effect of 63 species herbal medicine extracts on S. mutans growth by paper disc method and then examined the effects of S. mutans growth by some selected herbal medicines. Antibacterial effect of 63 species medicinal herb extracts on S. mutans was superior to Lycopi herba, Scutellariae radix in regular order and antibacterial activity(20 mg/ml concentration) through paper disc method were measured 6.3 mm and 3.5 mm, respectively. The growth of S. mutans in control medium was the highest at 8hrs, while that of medicinal herbs extract added-medium(2 mg/ml) was at 16 hrs. The pH values of the control media and media supplemented with Lycopi herba and Scutellariae radix extract were 5.08, 5.80, and 5.74 at 8 hrs, respectively. In the change of total carbohydrate of the control media and supplemented with Lycopi herba and Scutellariae radix extract were 0.81 mg/ml, 1.70 mg/ml and 1.84 mg/ml at 8 hrs, respectively. In the change of proteins of the control media and supplemented with Lycopi herba and Scutellariae radix extract were 8.39 mg/ml, 9.52 mg/ml and 9.28 mg/ml at 8 hrs, respectively. The polysaccharide contents of the control media and supplemented with Lycopi herba and Scutellariae radix extract were 300 mg/100 ml, 200 mg/100 ml and 220 mg/100 ml at 8hrs, respectively.

• Korean Journal of Oriental Medicine
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• v.2 no.1
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• pp.496-505
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• 1996

In order to improve the qualities of boiling extract the availability of standard compounds was investigated in extract of Scutellariae Radix. The standard compounds used baicalin(1), baicalein(2), and wogonin(3). The availabilities were analyzed by thin layer chromatography (TLC) and high performance liquid chromatography(HPLC) on $C_18$ column. The extract of RS-4(W/V=10g/100m1) showed the highest availabilities as 7.95% of baicalin(1), 1.04% of baicatein(2), and 0.31% of wogonin(3).

• Journal of Korean Medicine for Obesity Research
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• v.17 no.1
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• pp.20-28
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• 2017

Objectives: The purpose of this study was to investigate anti-obesity effect of Scutellariae Radix extract combined with metformin. Methods: Male C57BL/6 mice, 4 weeks of age, were used to set high-fat diet induced obesity model. They were grouped NOR (normal control), HFD (high fat diet control), MET (metformin, 100 mg/kg/day), MH2 (metformin 50 mg/kg/day+Scutellariae Radix 200 mg/kg/day), and HG4 (Scutellariae Radix 400 mg/kg/day). MET, MH2, and HG4 were orally administered for 10 weeks. Body weight was measured every week. Fasting blood glucose, triglyceride, total cholesterol (TC), high density lipoprotein, glutamic oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) were measured before sacrifice. Oral glucose tolerance test (OGTT) was also performed. Organ weight and internal fat weight were measured after sacrifice. Results: MH2 group showed a reduction of body weight when compared with HFD group. MH2 group showed stable blood level control which was calculated areas under the curves by OGTT. TC, GOT, GPT level, internal fat, and organ weight in MH2 group reduced. Conclusions: The combined treatment of Scutellariae Radix and Metformin has impact on treating obesity. Further studies are needed to evaluate the effect of Metformin and herbal medicine combination therapy.

• Journal of Physiology & Pathology in Korean Medicine
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• v.26 no.3
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• pp.325-329
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• 2012

The present study investigated the antimicrobial properties of medicinal herbs including Scutellariae Radix (SR: dried root of Scutellariae bicalensis Georgi). Among hot-water extracts of medicinal herbs tested in this study, SR extract showed the most potent antimicrobial activity with minimum inhibitory concentration (MIC) of 0.625 mg/mL. In particular, synergistic effects of antimicrobial activity were observed upon combined application of SR and chitooligosaccharide as indicated by MIC of 0.125 mg/mL and FIC (fractional inhibitory concentration) index of 0.45. Thermal stability analysis indicated that the components responsible for antimicrobial activity was stable for 8 months at $45^{\circ}C$. Antimicrobial activity was proven to be effective in foods as well as in cosmetics as comparable to that of the chemical preservatives.

• The Korea Journal of Herbology
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• v.35 no.5
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• pp.23-31
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• 2020

Objectives : This study was conducted to evaluate the anti-hyperlipidemia effect of Scutellariae Radix, Aucklandiae Radix and Bupleuri Radix(SAB). Methods : FL83B cells were mouse liver hepatocytes, and we used this cell line. FL83B cells were treated with 0.5 mM oleic acid(OA) for 24 h, SAB extract was treated. After OA treatment, intracellular triglyceride (TG) and free fatty acid contents were measured with AdiopoRed™ assay and Free Fatty Acid Quantitation assay kit, respectively. Further, we evaluated several lipogenesis and metabolic markers such as sterol regulatory element-binding transcription factor-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), 3-hydroxy3-methyl-glutaryl CoA reductase (HMGCR), hormone-sensitive lipase (HSL), carnitine palmitoyltransferase (CPT-1), peroxisome proliferator activated receptor alpha (PPARα), and cluster of differentiation (CD36) using RT-PCR and Western-blot analysis. Results : OA markedly increased intracellular TG and free fatty acid, which plays a key role in reducing hepatic lipid accumulation, in FL83B cells. These increases were alleviated by SAB extract. The mRNA and protein expression of Fatty acid(FA) oxidation factors (CPT-1, PPARα), lipolysis factor(HSL), FA transporter(CD36), cholesterol synthesis factors (HMGCoA) and Lipodenesis (SREBP-1c, FAS, and ACC-1) were significantly increased by treatment of SAB extract in the OA-induced fatty liver cell model. Conclusions : In summary, the treat of SAB extract showed a significant reduction of the influx of fatty acids into hepatocytes, promoted the oxidation of fatty acids, and regulated fat synthesis-related factors, thereby regulating the accumulation of TG and free fatty acids.