• 한국환경농학회지
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• 제36권4호
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• pp.269-278
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• 2017

BACKGROUND: The objective of this study was to evaluate screening method of residual multi pesticides in dead birds by Orbitrap high resolution mass spectrometry (HRMS) to identify the cause of death for birds. METHODS AND RESULTS: Extraction and clean-up method of residual pesticides in liver of dead birds was used QuEChERS (Quick Easy Cheap Effective Rugged and Safe) and method validations was conducted using liquid chromatography and gas chroamtography with triple-quadrupole mass spectrometer (LC/MS/MS and GC/MS/MS) Also, we were evaluated screening method for the determination of residual pesticides in liver of dead birds by LC and GC Orbitrap Mass Spectrometry. Results of method validations, Correlation coefficients of the matrix matched calibration curves were >0.978, and the method detection limits (MDLs) and limits of quantitation (LOQ) were 2.8~72.1 ng/g (18.4 ng/g on average) and 9.0~230 ng/g (58.5 ng/g on average). The accuracy ranged from 69.1%to 130% (103% on average), and the precision values were less than 14.8%(3.8%on average). The screening of residual pesticides in liver of dead birds by LC and GC Orbitrap HRMS was detected monocrotophos, carbofuran, carbosulfan, deltametrin, benfuracarb, carbofuran, phosphamidon, prochloraz in investigated samples. CONCLUSION: This results showed that accurate mass were extraction of residual pesticides in dead birds by Orbitrap HRMS. It suggested that this screening method is applicable to the residual pesticide analysis for the cause of death as a main tool.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제22권4호
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• pp.271-286
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• 2011

Objectives : The purposes of this study were to develop a mental health screening questionnaire and the practical intervention programs or guidelines which can be used in middle and high schools by students and teachers. Methods : The Adolescent Mental health & Problem behavior Screening Questionnaire II (AMPQ-II) was developed and was tested for reliability and validity using nationwide data from 2,180 middle and high school students and 2,145 teachers. Results : The AMPQ-II was measured by both students and teachers. The correlation coefficient of the test-retest test was 0.567 and internal consistency was high (Cronbach's alpha=0.878). Five factors such as worry and thought, mood and suicide, academic and Internet-related problems, peer problems, and rule violations were proposed. We also developed a practical guideline and manuals which can be easily used by secondary school teachers to screen for and manage mental health problems at school. Conclusion : The AMPQ-II can be a useful and reliable tool to screen for mental health problems in middle and high schools in South Korea.

• Weed & Turfgrass Science
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• 제4권2호
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• pp.118-123
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• 2015

메타게놈(metagenome)은 연구실에서 배양이 불가능한 미생물을 포함한 자연계에 존재하는 미생물의 유전자를 직접 연구하는 학문분야이다. 지구상 거의 모든 자연, 인공 환경에서 살고 있는 미생물 DNA를 분리 정제하는 것이 가능하며, 재조합 DNA 기술 등을 이용하여 배양 가능한 숙주미생물에 메타지놈을 클로닝함으로서 메타지놈 라이브러리를 제작할 수 있다. 최근 메타게노믹스를 통하여 자연계에 존재하고 있는 대다수의 미생물들이 실험실에서 배양되지 않았던 이유를 구명할 수 있게 되었고, 그들이 가지고 있는 생태학적인 의미와 역할에 대한 이해와 더불어 점차 그 응용 분야와 범위도 확대되고 있다. 이와 같은 메타게놈의 응용 분야 확대의 한 방안으로 본 연구에서는 새로운 제초활성 물질 및 제초활성 물질 생산에 필요한 유전자를 확보하기 위해 메타게놈 라이브러리를 대상으로 오이 떡잎절편 검정, 미세조류 생장저해 검정, 종자발아 저해 검정의 HTS (high throughput screening) 시스템을 구축하였고, 구축된 시스템으로부터 선발된 최종 단일 클론인 9-G1과 9-G12의 바랭이에 대한 in vivo assay를 통해 본 연구에서 개발한 HTS 시스템의 유효성을 확인 하였다. 후속연구로서 활성단일클론이 만들어내는 제초활성물질의 동정 및 제초활성물질을 합성하는 유전자군에 대한 연구를 수행 중에 있다.

• 대한지역사회영양학회지
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• 제27권3호
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• pp.205-222
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• 2022

Objectives: This study examined the characteristics of patients according to nutritional status assessed by five nutritional screening tools: Patient-Generated Subjective Global Assessment (PG-SGA), NUTRISCORE, Nutritional Risk Index (NRI), Prognostic Nutritional Index (PNI), and Controlling Nutritional Status (CONUT) and to compare the agreement, sensitivity, and specificity of these tools. Methods: A total of 952 gastric cancer patients who underwent gastrectomy and chemotherapy from January 2009 to December 2012 were included. The patients were categorized into malnutrition and normal status according to five nutritional screening tools one month after surgery. The Spearman partial correlation, Cohen's Kappa coefficient, the area under the curve (AUC), sensitivity, and specificity of each two screening tools were calculated. Results: Malnutrition was observed in 86.24% of patients based on the PG-SGA and 85.82% based on the NUTRISCORE. When NRI or CONUT were applied, the proportions of malnutrition were < 30%. Patients with malnutrition had lower intakes of energy and protein than normal patients when assessed using the PG-SGA, NUTRISCORE, or NRI. Lower levels of albumin, hemoglobin, total lymphocyte count, and total cholesterol and longer postoperative hospital stays were observed among patients with malnutrition compared to normal patients when NRI, PNI, or CONUT were applied. Relatively high agreement for NUTRISCORE relative to PG-SGA was found; the sensitivity was 90.86%, and the AUC was 0.78. When NRI, PNI, and CONUT were compared, the sensitivities were 23.72% for PNI relative to NRI, 44.53% for CONUT relative to NRI, and 90.91% for CONUT relative to PNI. The AUCs were 0.95 for NRI relative to PNI and 0.91 for CONUT relative to PNI. Conclusions: NUTRISCORE had a high sensitivity compared to PG-SGA, and CONUT had a high sensitivity compared to PNI. NRI had a high specificity compared to PNI. This relatively high sensitivity and specificity resulted in 77.00% agreement between PNI and CONUT and 77.94% agreement between NRI and PNI. Further cohort studies will be needed to determine if the nutritional status assessed by PG-SGA, NUTRISCORE, NRI, PNI, and CONUT predicts the gastric cancer prognosis.

• 대한간호학회지
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• 제16권3호
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• pp.67-77
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• 1986

The research was carried out in order to assess, at an early stage, the potential barrier in mother-child relationship in the childs' development by using the screening tool. Consequently, after modifying the Neonatal Perception Inventory developed by Broussard and testing its reliability. It has been applied to 152 mothers at the hospital of three universities in Seoul from August 1 to September 30, 1986. The data was analyzed by S.P.S.S. pro-gram and the results were as follows: 1) Mothers perceived the newborn as an individual, and evaluated their baby higher than other babies. 2) Mothers indicated difficulties in their role performance in the fellowing order-bathing, safety, detecting pain or suffering, and feeding. 3) Mothers' perception of a newborn was positive for 75% of the mothers. 4) The factor analysis of the modified tool using principal components analysis and Varimax rotation resulted in the two factors: Factor 1, Baby as an individual: Factor Ⅱ, Performances are required to meet the new-born's needs. 5) The difference in mothers' individual characteristics such as the number of childbirth, the desire to get pregnant or not, the type of deli-very, and the sex of the newborn did not influence on a mothers' perception of her newborn. As seen above, most of the mothers perceived their newborns as able individuals and expressed difficulties involved in taking care of the newborn. Also most of the mothers perceived their babies positively. My point here is, we ought to observe those mothers who perceive their babies negatively, and then compare them with those mothers who perceive their babies extremely positive or extremely negative. In the future, for more comprehensive assessment tool for maternal perception of the newborn, a repetitive verification and modification of this tool is demanded. At the same time, father's perception of the newborn should be included.

• 대한물리의학회지
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• 제18권2호
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• pp.41-48
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• 2023

PURPOSE: Falls are caused by a decline in physical and cognitive function. A quantitative evaluation tool that can comprehensively evaluate motor and cognitive functions for elderly people with an impaired physical function. This study assessed the clinical application potential by confirming the correlation between the physical function tests, cognitive and the stepping trail-making test (S-TMT). METHODS: Fourteen community-dwelling older patients (65-75 years) were recruited. The study conducted cognitive function tests with the trail-making test (TMT-A, B), and physical function tests (6-minute walking test (6MWT), short physical performance battery (SPPB), and timed up and go (TUG)). The results of were analyzed using SPSS version 21.0. Descriptive statistics were used for the general characteristics of the study subjects, and the correlations between S-TMT, other functional tests were examined through Pearson's correlation analysis. The statistical significance was set to .05. RESULTS: S-TMT had a significant positive correlation with the TUG (r = .588^*) and trail-making test-B (TMT-B) (r = .689^*, p < .05). Furthermore, S-TMT showed a negative correlation between SPPB (r = -.397) and 6MWT (r = -.422), but it was not statistically significant. CONCLUSION: S-TMT is a cognitive-gait dual-task performance evaluation tool that can be performed safely. A significant correlation was confirmed between the TUG test and the TMT-B. S-TMT is a dual-task screening tool that can evaluate both physical and cognitive functions simultaneously.

• 한국지하수토양환경학회지:지하수토양환경
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• 제16권1호
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• pp.32-41
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• 2011

By using a newly developed Korean risk-based corrective action (K-RBCA) software (K-RBCA) and the RBCA Tool Kit, risk assessment was performed on a site that was contaminated with aromatic hydrocarbons and heavy metals. Eight chemicals including benzene, ethylbenzene, xylenes, naphthalene, benz(a) anthracene, benzo(b) fluoranthene, benzo(a) pyrene, and arsenic that exceeded the US EPA Soil Screening Level were chosen as the target pollutants. A conceptual site model was constructed based on the site-specific effective exposure pathways. According to the RBCA Tool Kit the carcinogenic risk of arsenic was larger than $10^{-6}$, which is the generally acceptable carcinogenic risk level. The K-RBCA estimated the same level of carcinogenic risk for arsenic. With the RBCA Tool Kit, the carcinogenic risk of benzo(a) pyrene was estimated to be about $1.3{\times}10^{-6}$. However, with the K-RBCA benzo(a) pyrene did not exhibit any risk. The inconsistency between the softwares was attributed to the different fundamental settings (i.e., medium division) between the two softwares. While the K-RBCA divides medium into surface soil, subsurface soil, and groundwater, the RBCA Tool Kit divides medium into only soil and groundwater. These differences lead to the different exposure pathways used by the two softwares. The K-RBCA considers the exposure pathways in surface soil and subsurface soil separately to estimate risk, however, the RBCA Tool Kit considers the surface soil and subsurface soil as one and uses the integrated exposure pathways to estimate risk. Thus the resulting risk is higher when the RBCA Tool Kit is used than when the K-RBCA is used. The results from this study show that there is no significant difference in the risks estimated by the two softwares, thus, it is reasonable to use the K-RBCA we developed in risk assessment of soil and groundwater. In addition, the present study demonstrates that the assessor should be familiar with the characteristics of a contaminated site and the assumptions used by a risk assessment software when carrying out risk assessment.

• 대한감각통합치료학회지
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• 제16권3호
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• pp.23-33
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• 2018

목적 : 본 연구는 국내 아동 작업치료사들이 사용하고 있는 평가도구를 영역별로 구체화하여, 평가도구의 사용 동향을 파악하고자 하였다. 연구방법 : 본 연구는 아동 작업치료사를 대상으로 평가도구 사용에 대한 설문을 실시하였다. 평가도구의 하위영역은 작업수행, 일상생활활동, 교육, 놀이, 감각-지각, 운동 및 실행, 인지, 사회적 상호작용 기술, 발달, 이학적 검사로 구성하였다. 설문에 응답한 105명의 자료를 대상으로 기술통계 및 빈도분석을 사용하여 분석하였다. 결과 : 본 연구 결과는 초기 평가 시 소요되는 시간은 30~60분(41.9%)이 가장 많았으며, 재평가 주기는 3~6개월(41.0%)로 가장 많이 실시하는 것으로 나타났다. 영역 별 평가도구를 살펴보면, 작업 수행은 Canadian Occupation Performance Measure(55.2%)을, 일상생활활동은 Wee Functional Independence Measure(57.1%)을, 교육은 School Function Assessment(2.5%)를, 놀이 평가도구는 Revised Knox Preschool Play Scale(28.6%)을, 감각-지각은 Developmental Test of Visual Perception(94.3%)을, 운동 및 실행 평가도구는 Bruininks-Oseretsky Test of Motor Proficiency-2(42.9%)를, 인지 평가도구는 Lowenstein Occupational Therapy Cognitive Assessment(11.4%)를, 사회적 상호작용 기술은 Evaluation of Social Interaction(6.1%)을, 발달은 Denver Developmental Screening Test-II(92.4%)를, 이학적 검사는 임상관찰(89.5%)을 주로 사용하는 것으로 나타났다. 결론 : 본 연구를 통하여 국내 아동작업치료사들이 사용하고 있는 평가도구를 영역별로 구체적으로 파악할 수 있었으며, 이를 통해 추후 평가 교육 및 개발을 위한 기초적인 근거자료가 될 것으로 기대된다.

• Journal of Nutrition and Health
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• 제47권2호
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• pp.124-133
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• 2014

본 연구는 입원환자의 전자의무기록에서 용이하게 얻을 수 있는 지표들을 중심으로 초기영양평가를 신속하게 진행하기 위한 영양검색도구를 개발하고, 새로 개발한 영양검색도구에 대한 신뢰도을 검증하기 위한 목적으로 수행되었다. 이를 위하여 입원환자 903명을 대상으로, 의무기록 및 개별면담을 통해 자료를 수집하여 PGSGA를 기준으로 양호군과 영양불량군을 나누었다. 1) 로지스틱 회귀분석을 통해 양호군과 영양불량군으로 범주화하기 위한 최종 영양평가지표로 연령, BMI, 혈중 알부민, 혈중 콜레스테롤, 총단백질, 헤마토크리트, 체중변화, 섭취변화여부가 선정하였다. 특히 체중변화, 섭취변화여부의 경우는 예/아니오의 주관적 단답식 조사로 간단히 이루어졌다. 2) SMST의 영양평가결과 총 903명 중에서 양호군은 620명 (68.7%), 영양불량군은 283명 (31.3%)으로 나타났으며, 양호군과 영양불량군사이에 연령, 혈중 알부민, 총임파구수, BMI, 헤모글로빈, 헤마토크리트, 총단백질, 혈중 콜레스테롤, 재원기간 모두 유의적인 차이를 보였다. 3) SMST의 신뢰도를 검증하기 위해서 PGSGA와 NRS2002와의 민감도, 특이도, 카파비를 통한 일치도를 분석하였다. SM-ST는 PGSGA와 민감도 94.4%, 특이도 88.4%, 카파비 0.747로 'Substantial agreement'하며 (p < 0.001), NRS2002와 민감도 96.1%, 특이도 79.5%, 카파비 0.505로 'Moderate agreement' (p < 0.001)로 나타났다. 본 연구에서 개발한 새로운 초기영양검색 도구인 SMST의 영양평가지표는 의무기록 및 단답형의 질문으로 이루어져, 신속하고 간단한 초기영양평가가 가능하다. 특히 기존에 개인면담을 통해 시간적, 인력적 요구가 높은 영양도구들에 비해, 입원당시 기본적 신체계측과 함께 단답형으로 이뤄진 체중변화 및 식이섭취변화 여부가 기록될 경우 추가될 경우 추가조사 없는 의무기록으로 평가가 가능하다. SMST는 PGSGA, NRS2002와 높은 일치도를 보여 기존에 타당도가 높다고 알려진 두 영양검색 도구와 도구간 신뢰도가 검증되었다. 하지만 SMST는 합병증의 유무, 사망률, 재입원률, 병원비용 등 환자의 예후와 관련한 연관성 및 보편적 대상자에서의 유용성은 검증되지 않았으므로, 이에 관한 추가적인 연구가 필요하다고 사료된다.

• 한국보건간호학회지
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• 제26권3호
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• pp.532-546
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• 2012

Purpose: This study was conducted in order to investigate the level of intimate partner violence (IPV), beliefs and attitudes toward IPV and depression in victims of IPV and to identify factors influencing their depression. Methods: This was a descriptive study using a questionnaire. The Woman Abuse Screening Tool was used for examination of IPV, and the tool developed by Saunders, Lynch, Grayson & Linz(1987) and Haj - Yahia (2003) was used for examination of beliefs and attitudes toward IPV. The CES -D Scale was used for evaluation of depression. Results: A total of 172 victims were recruited. The level of IPV was low ($1.36{\pm}0.30$ out of 3) and the level depression was mild depression ($11.57{\pm}11.30$, out of 60). Beliefs and attitudes toward IPV were not positive (2.51 out of 5). A significant relationship was observed between beliefs and attitudes toward IPV, level of IPV and depression. Factor influencing depression was physical abuse, and that was explained by 13.5% (F=3.600, p=.001). Conclusion: According to these results, depression was a significant symptom in victims of IPV, therefore, health care providers should recognize depression as a significant indicator of IPV and should evaluate women with depression regarding their experience with IPV.