• Journal of Mechanical Science and Technology
• /
• 제19권6호
• /
• pp.1290-1303
• /
• 2005

In this research, a remote control system has been developed and implemented, which combines autonomous obstacle avoidance in real-time with force-reflective tele-operation. A tele-operated mobile robot is controlled by a local two-degrees-of-freedom force-reflective joystick that a human operator holds while he is monitoring the screen. In the system, the force-reflective joystick transforms the relation between a mobile robot and the environment to the operator as a virtual force which is generated in the form of a new collision vector and reflected to the operator. This reflected force makes the tele-operation of a mobile robot safe from collision in an uncertain and obstacle-cluttered remote environment. A mobile robot controlled by a local operator usually takes pictures of remote environments and sends the images back to the operator over the Internet. Because of limitations of communication bandwidth and the narrow view-angles of the camera, the operator cannot observe shadow regions and curved spaces frequently. To overcome this problem, a new form of virtual force is generated along the collision vector according to both distance and approaching velocity between an obstacle and the mobile robot, which is obtained from ultrasonic sensors. This virtual force is transferred back to the two-degrees-of-freedom master joystick over the Internet to enable a human operator to feel the geometrical relation between the mobile robot and the obstacle. It is demonstrated by experiments that this haptic reflection improves the performance of a tele-operated mobile robot significantly.

• 미생물학회지
• /
• 제31권3호
• /
• pp.197-202
• /
• 1993

출아법으로 분열하는 S. cerevisiae 는 mother cell 과 bud cell 과의 연결부위근처의 원형질막 내부에 10-nm filament ring 이 존재한다. CDC3, CDC10, CDC11, CDC12 유전자가 이 filament 를 암호화할 가능성이 많은 것으로 알려져 있으며, 근래에 CDC3 와 CDC12 유전자의 생성물들이 filament ring 에 존재한다는 것이 형광현미경을 이용하여 밝혀졌으나 그 기능은 밝혀지지 않았다. 이에 본인은 10-nm filament ring 의 지능을 알아보기 위하여 ring을 이루고 있는 S. cerevisiae 의 CDC3유전자와 유사한 S. pombe 유전자의 clone 을 시도하였다. 이를 위하여 .lambda. gt11 expression vector 에 S. pombe genomic library 를 만들고 CDC3 항채를 이용하여 screen 하였다. Screen 된 유전자를 sequencing 하여 본 결과, 2개 이상의 intron 이 존재하는 것이 밝혀졌으나, 일부 부위의 아미노산 서열과 CDC3 아미노산 서열을 비교하여 본 결과, 약 62%의 유사성이 존재하였다.

• BMB Reports
• /
• 제38권1호
• /
• pp.41-48
• /
• 2005

In this study we developed the transposon-mediated shuttle vector 'Hanpvid', which composed of HaNPV (Heliothis armigera nuclear polyhedrosis virus) genomic DNA and a transposon cassette from Bacmid of Bac-to-Bac system. Hanpvid replicates in E. coli in the same way as Bacmid and retains infective function in cotton bollworm cells (Hz-AM1). Using Hanpvid we constructed a recombinant virus, which could infect Hz-AM1 cells and generate recombinant HaNPV (rHa-Bar) containing the barnase gene, a ribonuclease gene from Bacillus amyloliquefaciens. Since the expression vector carrying barnase gene cannot replicate in the absence of barstar, a specific inhibitor of barnase, we constructed a new cotton bollworm cell line (AM1-NB) using the marker rescue method. In AM1-NB barstar was integrated into the cellular chromosome to sustain the replication of rHa-Bar. To screen out recombinant HaNPV for potential use as biopesticide, Hz-AM1 and AM1-NB cell lines were infected with rHa-Bar, respectively. The results obtained indicate that Viral progenies in AM1-NB were 23 and 160 times greater than those in Hz-AM1 48 h and 72 h after infection, respectively. With additional insertion of the polyhedron gene from AcNPV (Autographa californica nuclear polyhedrosis virus) into the Hanpvid genome, rHa-Bar regained the polyhedron phenotype and its pest-killing rate greatly improved. Toxic analysis showed that the lethal dosages ($LD_{50}$) and the lethal time(s) ($LT_{50}$) of rHa-Bar were reduced by 20% and 30%, respectively, compared to wt-HaNPV in the third instar larvae of cotton bollworm. This study shows that in AM1-NB barnase can be effectively produced and used as pest-killing agent for the biological control of cotton pests.

• 센서학회지
• /
• 제19권2호
• /
• pp.149-154
• /
• 2010

Top of the alumina body which is made according to plan has been printed using a screen printer equipment in order to print an electrode pattern. The electrode is connected with the quartz crystal oscillator of the piezoelectricity method which has a piezoelectric characteristic. The pressure contact department has been experimented using three type of the teflon. The teflon is good for pressure delivery vector but it is bad restitution. So we devised the structure which inserts the metal in the teflon. Bottom of the module is connected with the signal processing department which is planned in advance and then has investigated to characteristic. Therefore we have got the best output-voltage and frequency characteristic follows in the pressure.

• Molecules and Cells
• /
• 제23권2호
• /
• pp.170-174
• /
• 2007

High-throughput subcellular imaging is a powerful tool for investigating the function of genes. In order to identify novel regulators of apoptosis we transiently transfected HeLa cells with 938 hypothetical genes of unknown function, and captured their nuclear images with an automated fluorescence microscope. We selected genes that induced greater than 3-fold increase in the percentage of apoptotic nuclei compared with vector-transfected cells. The full-length genes C10orf61, MGC 26717, and FLJ13855 were identified as candidate proapoptotic genes, and their apoptotic effects were confirmed by DNA fragmentation ELISAs and Western blotting for caspase-7 and PARP. We conclude that a subcellular image-based apoptotic screen is useful for identifying genes with proapoptotic activity.

• ETRI Journal
• /
• 제37권4호
• /
• pp.743-751
• /
• 2015

Cloud gaming services are heavily dependent on the efficiency of real-time video streaming technology owing to the limited bandwidths of wire or wireless networks through which consecutive frame images are delivered to gamers. Video compression algorithms typically take advantage of similarities among video frame images or in a single video frame image. This paper presents a method for computing and extracting both graphics information and an object's boundary from consecutive frame images of a game application. The method will allow video compression algorithms to determine the positions and sizes of similar image blocks, which in turn, will help achieve better video compression ratios. The proposed method can be easily implemented using function call interception, a programmable graphics pipeline, and off-screen rendering. It is implemented using the most widely used Direct3D API and applied to a well-known sample application to verify its feasibility and analyze its performance. The proposed method computes various kinds of graphics information with minimal overhead.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권11호
• /
• pp.6557-6562
• /
• 2013

Objective: Specific promoters could improve efficiency and ensure the safety of gene therapy. The aim of our study was to screen examples for lung cancer. Methods: The firefly luciferase gene was used as a reporter, and promoters based on serum markers of lung cancer were cloned. The activity and specificity of seven promoters, comprising CEACAM5 (carcinoembryonic antigen, CEA), GRP (Gastrin-Releasing Peptide), KRT19 (cytokeratin 19, KRT), SFTPB (surfactant protein B, SP-B), SERPINB3 (Squamous Cell Carcinoma Antigen, SCCA), SELP (Selectin P, Granule Membrane Protein 140kDa, Antigen CD62, GMP) and DKK1 (Dickkopf-1) promoters were compared in lung cancer cells to obtain cancer-specific examples with strong activity. Results: The CEACAM5, DKK1, GRP, SELP, KRT19, SERPINB3 and SFTPB promoters were cloned. Furthermore, we successfully constructed recombinant vector pGL-CEACAM5 (DKK1, GRP, SELP, KRT19, SERPINB3 and SFTPB) contained the target gene. After cells were transfectedwith recombinant plasmids, we found that the order of promoter activity from high to low was SERPINB3, DKK1, SFTPB, KRT19, CEACAM5, SELP and GRP and the order for promoters regarding specificity and high potential were SERPINB3, DKK1, SELP, SFTPB, CEACAM5, KRT19 and GRP. Conclusion: The approach adopted is feasible to screen for new tumour specific promoters with biomarkers. In addition, the screened lung-specific promoters might have potential for use in lung cancer targeted gene therapy research.

• 한국정보통신학회:학술대회논문집
• /
• 한국해양정보통신학회 2003년도 춘계종합학술대회
• /
• pp.281-284
• /
• 2003

인터넷의 급속한 발전은 표현의 고급화와 보편성을 제공하는 웹을 주축으로 하여 이루어져 왔으나, 웹 상에서 표현중심의 그래픽처리에는 비구조적인 방법에 따른 많은 문제점을 가지고 있다. 이러한 문제점을 보완하고자 W3C에서는 웹 상에서 벡터그래픽 표현의 효율적인 표현 및 저장, 공유를 가능하게 하기 위해 XML 어플리케이션인 SVG를 제정하였다. 이에 본 논문에서는 SVG 문서를 이용하여 그래픽 편집기 형태로 SVG의 구조적 편집 및 화면에 표현되는 그래픽 객체를 직접 편집할 수 있는 인터페이스를 통해 벡터그래픽을 편집하고 표현하기 위한 SVG 편집시스템을 설계하였다. 본 시스템은 지리정보시스템이나 서비스 환경 등에서 XML기반의 그래픽을 표현하는 효율적인 처리 등에 유용하게 사용할 수 있을 것이다.

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1997년도 춘계학술대회
• /
• pp.80-80
• /
• 1997

The object of this study was to develop a gene therapy strategy for ovarian cancer. We have previously shown that AV with a L-plastin (LP) promoter infects breast and ovarian cancer cells and expressed ${\beta}$-galactosidase cDNA in preference to normal fibroblast cells and hematopoietic cells. We now report on the cytotoxicity of Ad.LP.CD, an AV carrying a CD cDNA which converts the pro-drug, 5-Fluorocytosine (5-FC) into the toxic drug 5-Fluorouracil (5-FU). Infection of Ad.LP.CD into either 293 cells or ovarian cancer cells generated the functional CD as measured by HPLC analysis. Using a ratio of AV to OVCAR3 cell of 100 and a 5-FC concentration of 100 ${\mu}$M, we achieve an over 95 % of cell growth inhibition. We are using flow cytometry analysis for ${\beta}$ -galactosidase and ovarian cancer associated folate receptor to screen primary ascites samples for infectivity after infection with an adenoviral vector, i.e., Ad.LP.LacZ. This vector system may be of value in the treatment of microscopic disease of ovarian cancer in the peritoneal cavity.

• ETRI Journal
• /
• 제34권4호
• /
• pp.542-552
• /
• 2012

In this paper, a gaze estimation method is proposed for use with a large-sized display at a distance. Our research has the following four novelties: this is the first study on gaze-tracking for large-sized displays and large Z (viewing) distances; our gaze-tracking accuracy is not affected by head movements since the proposed method tracks the head by using a near infrared camera and an infrared light-emitting diode; the threshold for local binarization of the pupil area is adaptively determined by using a p-tile method based on circular edge detection irrespective of the eyelid or eyelash shadows; and accurate gaze position is calculated by using two support vector regressions without complicated calibrations for the camera, display, and user's eyes, in which the gaze positions and head movements are used as feature values. The root mean square error of gaze detection is calculated as $0.79^{\circ}$ for a 30-inch screen.