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Identification of Novel Regulators of Apoptosis Using a High-Throughput Cell-based Screen  

Park, Kyung Mi (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kang, Eunju (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Jeon, Yeo-Jin (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim, Nayoung (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim, Nam-Soon (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Yoo, Hyang-Sook (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Yeom, Young Il (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim, Soo Jung (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Molecules and Cells / v.23, no.2, 2007 , pp. 170-174 More about this Journal
Abstract
High-throughput subcellular imaging is a powerful tool for investigating the function of genes. In order to identify novel regulators of apoptosis we transiently transfected HeLa cells with 938 hypothetical genes of unknown function, and captured their nuclear images with an automated fluorescence microscope. We selected genes that induced greater than 3-fold increase in the percentage of apoptotic nuclei compared with vector-transfected cells. The full-length genes C10orf61, MGC 26717, and FLJ13855 were identified as candidate proapoptotic genes, and their apoptotic effects were confirmed by DNA fragmentation ELISAs and Western blotting for caspase-7 and PARP. We conclude that a subcellular image-based apoptotic screen is useful for identifying genes with proapoptotic activity.
Keywords
Apoptosis; Automated Fluorescence Microscope; Hypothetical Genes; Subcellular Imaging;
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