• Title/Summary/Keyword: Scilla scilloides

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Cytotoxicity and Quantitative Analysis of Nortriterpenoid glycoside from Scilla scilloides (무릇에서 분리한 nortriterpenoid glycoside의 암세포에 대한 세포독성 및 함량 분석)

  • Lee, Sang-Myung;Chun, Hyo-Kon;Lee, Choong-Hwan;Lee, Ho-Jae;Kang, Shin-Jyung;Maeng, Hack-Young;Kho, Yung-Hee
    • Korean Journal of Pharmacognosy
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    • v.32 no.3 s.126
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    • pp.189-192
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    • 2001
  • Scillascilloside E-3 (1) and E-1 (2) were isolated from the bulbs of Scilla scilloides. The cytotoxic activities of these compounds were tested against murine (B16/F-10, 3LL) and human cancer cell lines (MCF7, PC-3, HT29, LOX-IMVI, A549 and HT1080). These compounds exhibited a significant cytotoxic activities against all tested cancer cells. Futhermore, the contents of 1 and 2 in S. scilloides are 43.2 and 27.9 mg/kg, respectively.

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Cytogenetic Studies of Scilla scilloides Complex from Korea III. Karyotype of Cytotype BB and B-Chromosome Composition (한국 무릇(Scilla scilloides Complex)의 세포유전학적 연구 III. BB 게놈의 핵형과 B염색체 조성)

  • 방재욱
    • Journal of Plant Biology
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    • v.36 no.3
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    • pp.281-284
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    • 1993
  • A karyotype of cytotype BB plant in Scilla scilloides Complex was established and the frequency of B-chromosomes were investigated. Chromosome complements of BB genome were composed of five pairs of subtelocentric and four pairs of metacentric chromosomes. Chromosome 1 has satellite with nucleolar organizer. Polymorphism was found in chromosome 2. The karyotype of cytotype BB will be available for analysis of genome composition in various cytotypes of S. scilloides Complex. The frequency of B-chromosome was 78.6%. Numbers of B-chromosome ranged from 1 to 4 and plants with 2B-chromosomes were predominant (57.2%). Two type of B-chromosomes, F and F', were found; F is a large iso-chromosome and F' a small one.

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Antimicrobial, Anti-inflammatory, and Anti-oxidative Activities of Scilla scilloides (Lindl.) Druce Root Extract

  • Yeo, Eun-Ju;Kim, Kee-Tae;Han, Ye-Sun;Nah, Seung-Yeol;Paik, Hyun-Dong
    • Food Science and Biotechnology
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    • v.15 no.4
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    • pp.639-642
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    • 2006
  • The root extract of Scilla scilloides (which has been used as a traditional folk medicine in Korea) was evaluated with regard to antimicrobial, anti-inflammatory, and anti-oxidative activities. The roots of S. scilloides were minced and extracted with 95% ethanol (root:ethanol=25:75, w/v). The inhibitory effects of S. scilloides root extract on the growth of Staphylococcus aureus ATCC 35556, Salmonella enteritidis ATCC 12021, Escherichia coli O157:H7, and Candida parapsilosis KCCM 35428 were tested. The results indicate that the antimicrobial effects of both 0.1 and 1.0% extract of S. scilloides were greater against the growth of S. aureus ATCC 35556 and C. parapsilosis KCCM 35428 than the growth of S. enteritidis ATCC 12021 and E. coli O157:H7. The anti-inflammatory effects were evaluated by measurement of the inhibition of hyaluronidase activity in vitro. It appears that both 0.1 and 1.0% concentrations of extract have inhibitory effects on hyaluronidase relative to the control. Finally, the anti-oxidative effect of 1.0 and 10% extract solutions were measured according to the thiocyanate method and were compared with 1.0% BHT. The results indicate that the anti-oxidative effect of 10% S. scilloides root extract (anti-oxidative index (AOI); $72.3{\pm}4.2$) is not significantly different from that of 1.0% BHA (AOI; $76.8{\pm}3.5$) (p<0.05). However, it appears that the anti-oxidative effect of S. scilloides root extract is at least three-fold greater than that of BHA when accounting for the amount of dissolved solids in each.

Genetic Analysis of Somaclones Derived from Different Cytotype Plants of Scilla scilloides Complex using RAPD (PCR-RAPD 기법을 이용한 세포 유형이 다른 무릇 (Scilla scilloides Complex) 체세포클론의 유전적 분석)

  • 오정순;방재욱
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.4
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    • pp.235-240
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    • 1999
  • The usefulness of randomly amplified polymorphic DNA (RAPD) was evaluated to access the genetic variation in somaclones derived from different cytotype plants of Scilla scilloides Complex, AA (2n=16), BB (2n=18) and AABB (2n=34). Three arbitrary decamer primers were successfully used to amplify genomic DNA from the somaclones. DNA polymorphism was observed between cytotypes. The total number of bands in AA, BB and AABB somaclones were 110, 116 and 103, and marker bands examined were 15, 19 and 26, respectively. The diversity of types using PCR in AA, AABB and BB somaclones were 39.2%, 72.3% and 45.7%, respectively. RAPD band patterns suggest that type AA is more stable than type BB and AABB. The frequencies of specific band in AA, BB or AABB somaclones were 0.9%, 4.3% and 4.9%, respectively. The applicability and reliability of RAPD markers for evaluating the somaclones are discussed.

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Chromosome Variation in Callus Cells Derived from Different Cytogenetic Type Plants of Scilla scilloides Complex (세포유전적 유형이 다른 무릇(Scilla scilloidise Complex)에서 유도된 캘러스 세포의 염색체 변이)

  • Jae-Wook BANG;Jae-hyun PARK;Eun-Young Choi
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.1
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    • pp.59-63
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    • 1994
  • Chromosome variation in callus cells initiated from different cytogenetic type plane of Scilla scilloides Complex was analysed Considerable variation in both chromosome number and struchure was found in type AA, while no autosomal variation was detected in type BB callus cells. In allotetraploid AABB, two hypoploid cells were fount while a hypoploid cell and three hyperploid cells were found in eutetraploid cells of BBBB. Autosomes in callus cells derived from the plant with B-chromosomes were more stable than those from the plant without B-chromosome. We doubt that B-chromosomes have a selective function for the autosomes in culture of S. scilloides Complex.

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Cytogenetic Studies of Scila scilloides Complex from Korea I. Distribution of Genomes and Composition and Frequencies of B Chromosome (한국 무릇(Scilla scilloides Complex)의 세포유전학적 연구 I. 게놈에 따른 분포 및 B염색체의 조성과 출현 빈도)

  • 최혜운
    • Journal of Plant Biology
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    • v.33 no.4
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    • pp.237-242
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    • 1990
  • Geographical distribution of diploid plant with AA genome (2n=16) and allotetraploid with AABB genome (2n=34) of Scilla scilloides Complex from Korea has been studied. The composition and frequencies of B chromosomes ere also investigated. Plants with AABB genome were predominant over AA genome plants. A mixed population of AA and AABB genome plants was found for the first time. Aneuploid plants have not been found. Chromosomes of AA genome were composed of three pairs of metacentric, two pairs of submetacentric, two pairs of subtlocentric and one pair of telocentric chromosomes, whereas BB genome was four pairs of metacentric and five pairs of subtelocentric chromosomes. B chromosomes were classified into two categories, isochromosome (F) and chromosome fragment (f). The frequencies of B chromosomes were 43% in AA genome plants and 44% in AABB genome plants. The number of B chromosome ranged from 1 to 3 and 1 to 7 in AA and AABB genome plants, respectively. B chromosome combinations were F and F+f in AA genome plants and F, F+f and f in AABB genome plants.

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