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Restricted Blood Flow Exercise in Sedentary, Overweight African-American Females May Increase Muscle Strength and Decrease Endothelial Function and Vascular Autoregulation

  • Bond, Vernon;Curry, Bryan Heath;Kumar, Krishna;Pemminati, Sudhakar;Gorantla, Vasavi Rakesh;Kadur, Kishan;Millis, Richard Mark
    • Journal of Pharmacopuncture
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    • v.20 no.1
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    • pp.23-28
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    • 2017
  • Objectives: Exercise with partially restricted blood flow is a low-load, low-intensity resistance training regimen which may have the potential to increase muscle strength in the obese, elderly and frail who are unable to do high-load training. Restricted blood flow exercise has also been shown to affect blood vessel function variably and can, therefore, contribute to blood vessel dysfunction. This pilot study tests the hypothesis that unilateral resistance training of the leg extensors with partially restricted blood flow increases muscle strength and decreases vascular autoregulation. Methods: The subjects were nine normotensive, overweight, young adult African-Americans with low cardiorespiratory fitness who underwent unilateral training of the quadriceps' femoris muscles with partially restricted blood flow at 30% of the 1-repetition maximum (1-RM) load for 3 weeks. The 1-RM load and post-occlusion blood flow to the lower leg (calf) were measured during reactive hyperemia. Results: The 1-RM load increased in the trained legs from $77{\pm}3$ to $84{\pm}4 kg$ (P < 0.05) in the absence of a significant effect on the 1-RM load in the contralateral untrained legs (P > 0.1). Post-occlusion blood flow decreased significantly in the trained legs from $19{\pm}2$ to $13{\pm}2mL{\cdot}min^{-1}{\cdot}dL^{-1}$ (P < 0.05) and marginally in the contralateral untrained legs from $18{\pm}2$ to $16{\pm}1mL{\cdot}min^{-1}{\cdot}dL^{-1}$ (P = 0.09). Changes in post-occlusion blood flow to the skin overlying the trained and the contralateral untrained muscles were not significant. Conclusion: These results demonstrate that restricted blood flow exercise, which results in significant gains in muscle strength, may produce decrements in endothelial dysfunction and vascular autoregulation. Future studies should determine whether pharmacopuncture plays a role in treatments for such blood vessel dysfunction.

Influence of Increased Temperature on the Standard Metabolism in the Marine Bivalves Acclimated to Seasonal Water Temperature -I. Effects of Acclimation Temperature (해산패류의 계절별 표준대사에 미치는 승온 효과 -I. 순화온도의 영향-)

  • Kim Kyoung Sun;Chin Pyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.5
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    • pp.463-468
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    • 2002
  • Influence of increased temperature on the standard metabolism in three species of marine bivalves, Crassostrea gigas, Ruditapes philippinarum and Mytilus edulis, acclimated to seasonal water temperatures and collected from the south coast of Korea, were examined in the laboratory. The standard oxygen consumption and filtration rates in the 3 species were measured respectively at the experimental temperature, 4, 7 and 10$^{\circ}C$ or 3, 6 and 9$^{\circ}C$ higher than the mean seasonal water temperature. When the experimental temperatures were higher than the seasonal water temperature, the rates of C. gigas decreased in autumn and spring, and increased In winter, while there was thermal stress in summer. The rates of R. philippinarum increased in spring when the experimental temperatures were 3$^{\circ}C$ and 6$^{\circ}C$ higher than the seasonal water temperature, but the rates increased in autumn and winter when the experimental temperature was even 9$^{\circ}C$ higher than the seasonal water temperature. In summer. metabolic activities of R. philippinarum decreased significantly at temperature higher than acclimation temperature. The rates of M. edulis increased in spring when the experimental temperatures were 3$^{\circ}C$ higher than the seasonal water temperature but the rates were stressed by the increased temperature above 3$^{\circ}C$. In winter, increased temperature did not affect the metabolic activities of M. edulis. These results suggested that the standard metabolism of the three marine bivalves in summer was stressed by the increased temperature, whereas the metabolism was activated in winter.

Experimental Studies on the Mechanism of Reproductive Cycle in the Bluegill, Lepomis macrochirus (파랑볼우럭, Lepomis macrochirus의 생식기구에 관한 실험적 연구)

  • LEE Taek Yuil;Kim Sunng Yeon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.6
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    • pp.489-500
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    • 1987
  • Annual reproductive cycle of Bluegill, Lepomis macrochirus (RAFINESQUE) were studied in the natural population. Based on these informations, reproductive mechanism of the fish including activation, degeneration and remature were examined under the controlled conditions of temperature and photoperioe. In the natural populations, gonads began to grow with the temperature increase in March ana matured in June, and spawning occurred in July. With the onset of the shorter day-length and the maximum temperature condition in August, the gonads began to degenerate. Resting stage was continued during winter season. In the laboratory-reared population, activation of the gonads was initiated by the complex environmental factors including higher temperature$(>15^{\circ}C)$ and longer photoperiod $(>14L)$. For the maturation, photoperiod of more than 14 hours was critical. Under this condition higher temperature was the only compensative factor. Regeneration of the gonads was induced by higher temperature$(>25^{\circ}C)$ and the shorter photoperiod accelerated the regenerative processes. Even from the resting stage the gonads can be induced to matured stage by the longer photoperiod $(>15L)$. Based on these observations, the reproductive rhythm of this fish is supposed to be artificially controlled.

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STUDIES ON THE ARTIFICIAL FERTILIZATION AND DEVELOPMENT OF CYCLINA SINENSIS (가무락 Cyclina sinensis의 인공수정 및 발생에 관한 연구)

  • CHOI Shin Sok;SONG Yong Kyoo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.6 no.1_2
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    • pp.76-80
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    • 1973
  • Cyclina sinensis is an edible bivalve inhabiting wide tidal flats which are exposed to the air at ebb tide along the western coast of Korea. Over the period of June to September 1971, some specimens from a tidal flat near Inchon were submerged in sea water with various concentrations of ammonium hydroxide added and careful observations were made on their fertilization, early development, and metamorphosis of the larvae. The highest rate of fertilization was demonstrated by individuals treated with 1/1000 normal solution of ammonium hydroxide and their fertilized eggs followed normal development, i.e., two cell stage 1.5 hours after fertilization, blastular stage after 4 hours, and trochophore stage after 6 hours. Within 24 hrs after fertilization C. sinensis larvae have acquired the form of early straight-hinge veliger with the mean prodissoconch I with the length of $110\mu$. It takes seven days to get the umbo stage with the mean shell length of $190\mu$ and twenty days to get the morphosing stage with the mean shell length of $260\mu$. The larvae were cultured to the metamorphosing stage with the shell length of $270\mu$ in the laboratory condition.

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The Ammonia Removal Capacity of a Few Kinds of Filter Media in a Water Reuse quaculture System (순환 여과식 양식 시설에 이용될 수종의 여과 재료의 효능에 관한 연구)

  • KIM In-Bae;KIM Pyong-Kih;CHEE Young-Ok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.6
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    • pp.561-567
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    • 1987
  • The purpose of this study was to find out the removal capacity of harmful ammonia by different filter media in the submerged biological filters in a given space of chamber. Four materials, pile cloth, corrugated skylight roofing plate, embossed plastic plate, and gravel, were used as the experimental filter media. Each filter medium was placed in two aquariums, each aquarium measuring $90cm\times60cm\times60cm\;(depth)$. Under the normal operating condition, the average of mean ammonia removal rates during the first and second functioning periods by each filter material which occupied tile space in the filter chamber (aquarium) was as follows: 1. Pile cloth: $8.381\;g{\cdot}m^{-3}.\;day^{-1}$ 2. Corrugated skylight roofing plate: $7.834\;g{\cdot}m^{-3}.\;day^{-1}$ 3. Embossed plastic plate: $7.797\;g{\cdot}m^{-3}.\;day^{-1}$ 4. Gravel: $7.051\;g{\cdot}m^{-3}.\;day^{-1}$ Thus, there were no significant differences between the media, but at the time of practical application of these materials, some other factors such as investment cost, easiness for the removal of excess detritus accumulated in tile interstices of filter media, etc. should be fallen into consideration. When large units are required, in particular, removal of excess detritus from tile gravel bed is extremely difficult, and in case of pile cloth filters the installation work is much complicated and a problem in supporting the structure when drained also exists. In these respects, corrugated skylight roofing plate and embossed plastic plate seem to be more optimal, but again in practice the local situation for the availability and the price of the materials should be rechecked and the fitness of tile materials in the particular filter chambers under use or under consideration for construction must be taken into account.

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Mathematical Approaches Related to Daily Feeding Activities of Rock Trout, Agrammus agrammus (노래미, Agrammus agrammus의 일주섭식활동에 관한 수리적 접근)

  • KIM Chong-Kawn;KANG Yong-Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.24 no.5
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    • pp.315-326
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    • 1991
  • This study was tried for a mathematical approaches related to daily fluctuations in the stomach fullness of Agrammus agrammus. The specimen was collected by angling and gill net from September 1984 to August 1985 off Shinsudo, Samchonpo. Fullness of the stomachs was increased in the early morning and the late afternoon, decreased in the late morning, at noon, and during the night. That is, feeding activity of the fish was more intense at sunrise and sunset. Daily feeding activity of the fish in a day was divided into the time period of gastric evacuation and both feeding and gastric evacuation. Stomach fullness with the lapse of time in the time period of gastric evacuation was geometrically decreased. Stomach fullness in the time period of both feeding and gastric evacuation was affected by gastric evacuation rates, feeding rates, and maximum fullness of the stomach. These parameters were able to estimate from the method estimating the regression coefficient in the relationship between the time(t) and the stomach fullness$(F_t)$, or between stomach fullness at the time t and $t+\Deltat$. The rates of feeding and gastric evacuation were the highest in spring and the lowest in winter. The relationships between time(t) and stomach fullness$(F_t)$ in the time period of gastric evacuation and both feeding and gastric evacuation induced from hypotheses were respectively as follows. $$F_t=F_{to}e^{-r(t-to)}$$, $$F_t=F\infty-(F\infty-F_{to})e^{-(p+r)(t-{to})}$$

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Effect of Acupuncture on Sciatica in Rat Models: Systematic Review and Meta-analysis (좌골신경통 백서모델에서 침치료의 효과: 체계적 문헌고찰 및 메타분석)

  • Yoon, Ye-Ji;Kim, Soojeon;Cho, Jae-Heung;Kim, Koh-Woon;Song, Mi-Yeon
    • Journal of Korean Medicine Rehabilitation
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    • v.30 no.1
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    • pp.79-93
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    • 2020
  • Objectives While sciatic neuropathy is one of the common symptoms which have the lifetime incidence of 13~40%, still there is no consensus about the standardized and the most effective conservative treatment. In addition, the importance of systematic review and meta-analysis of preclinical are growing as they could suggest possible effective treatment strategy for future studies. Therefore, we conducted systematic review and meta-analysis to estimate analgesic effect of acupuncture on sciatica in rat models. Methods Systematic search was conducted for all controlled comparative preclinical trials which assessed analgesic effect of acupuncture in sciatica rat models. Database of PubMed, EMBASE, Web of Science, CNKI and 6 Korean databases were used. The primary outcome was pain, which is evaluated by stimulus behavior tests in rat models. We assessed the methodological quality with Systematic Review Centre for Laboratory Animal Experimentation's risk of bias tool. RevMan 5.3 was used for meta-analysis and subgroup analysis was conducted according to treatment site, acupuncture point, treatment period and frequency used in electroacupuncture. Results 14 studies were finally included following our inclusion criteria. The data from meta-analysis indicated that the acupuncture significantly improved the result values of behavior tests for pain evaluation, compared to no-treatment control group in animal models (standardized mean difference=4.43, 95% confidence interval 3.16 to 5.69, Z=6.84, p<0.00001; χ2=68.02, p<0.00001; I2=82%). The results of subgroup analysis indicate that acupuncture treatment of unilateral site, distal acupoints, longer treatment period and applying 2/100 Hz frequency in electroacupuncture could be more effective. Conclusions Systematic review and meta-analysis of animal studies are getting important for the future clinical studies and the improvement of heatlh care. Therefore the results of the study would provide evidence and better design for the forthcoming studies.

A study of illumination of dental laboratories (치과 기공실의 조명에 관한 실태 조사 연구)

  • Lee, Hyo-Jung;Choi, Jong-In;Kim, Sung-Sook;Dong, Jin-Keun
    • Journal of Dental Rehabilitation and Applied Science
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    • v.30 no.4
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    • pp.289-298
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    • 2014
  • Purpose: This study was designed to investigate the present conditions of illumination techniques in dental laboratories in order to distinguish colors for esthetic dentistry. Materials and Methods: A total of forty-eight local dental laboratories were selected for sampling a) luminous intensity and b) color temperature. We measured the luminous intensity and the color temperature on a center point of porcelain table with lux meter and color meter respectively. The measurements were performed twice, once on a clear day and on a cloudy day between 12 pm and 2 pm (day light and artificial illumination) and between 7 pm and 10 pm (artificial illumination only). Results: The results obtained were as follows. 1. The average luminous intensity in dental laboratories lighting was 1871 lx, and 67% of dental laboratories had luminous intensity greater than 1600 lx, which was appropriate for operations related to colors, while 33% of dental laboratories had in sufficient lighting for appropriate luminous intensity. 2. The average color temperature in dental laboratories lighting was 6506 K, which was inappropriate for operations related to color. None of the dental laboratories had a color temperature range of 5000 - 5500 K, which is appropriate for operations related to colors. 3. Between daytime and nighttime, also between clear day and cloudy day, the average luminous intensity and color temperature in dental laboratories were not significantly different. Conclusion: Illumination in most dental laboratories selected in this study was inappropriate for operations related to color.

Tissue Culture of Grateloupia acuminata (Rhodophyta) from the Eastern Coast of Koea (동해안 홍조 지누아리사촌(Grateloupia acuminata) 식물의 조직배양)

  • Kim Hyung-Geun;Park Joong-Goo
    • Journal of Aquaculture
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    • v.19 no.3
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    • pp.216-221
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    • 2006
  • The Effect of different temperatures and photon irradiance on the growth of crust and the regeneration of tissue fragments of the commercially important red alga Grateloupia acuminat Okamura were examined in laboratory cultures. The tetraspore developed into basal crusts and produced upright thalli. Crust grew very fast at $25^{\circ}C$ and $80{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ after one week in indoor culture. However, they stopped growing after three weeks. Maximum growth was $275{\mu}m$ in diameter. They required four weeks to get upright thalli at $5^{\circ}C$, while only three weeks were required at $10^{\circ}C$. When different light intensities were compared at $15^{\circ}C$, cells of the crusts were well differentiated $80{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and consistently divided so that upright thalli were produced. In aeration culture, the upright thalli grew up to 6.5 cm in length within 4 months. Thus, it is possible to produce mass cultures of Grateloupia in the field. In addition, female and male gametophytes developed from the tetraspores and they were fertilized to produce tetrasporohyte thalli. By this procedure, the normal life cycle of the red alga G. acuminata was completed.

Quantitative Requirements of Copper and Manganese in Formulated Diets and Its Interrelation with Other Minerals in Young Eel (뱀장어용 배합사료의 적정 Cu와 Mn 첨가량에 관한 연구)

  • PARK Chul Won;SHIMIZU Chiaki
    • Journal of Aquaculture
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    • v.1 no.2
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    • pp.109-119
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    • 1988
  • Following the previous study on the nutritional quantity of Al, Fe, and their interrelationships with other trace metals, this study was conducted to determine the effect of supplementation of Cu and Mn to fish meal-diets on the growth of Japanese eel. The feeding experiment to determine Cu requirement was conducted using white fish meal-diets supplemented with 0, 5, 10, or 20 $\mu$g/g cupric sulfate, and that to determine Mn requirement was conducted by suppling the diet with 0, 10, 20, or 40 $\mu$g/g manganese sulfate. The results revealed that the fish on diet annexed with 5 $\mu$g/g of Cu were observed to have the best growth among these groups. In case of Mn, 20 $\mu$g/g of supplementary level promoted growth rate and 40 $\mu$g/g of diet resulted in the highest feed efficiency. On the other hand, the highest level of Cu (20 $\mu$g/g), and Mn (40 $\mu$g/g) supplementary diets did not expressed adverse effect on growth. These results indicated that the suitable Cu and Mn supplementary concentrations are 20 $\mu$g/g and 30 $\mu$g/g of formulated the white fish meal-diet respectively. The white fish meal had 1.6 $\mu$g/g of Cu and 6.1 $\mu$g/g of Mn.

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