• Title/Summary/Keyword: School complex

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Cloning of pdh Genes Encoding Subunits of Pyruvate Dehydrogenase Complex from Lactobacillus reuteri ATCC 55739

  • Nam, Su-Jin;Park, Jae-Yong;Kim, Jung-Kon;Ha, Yeong-Lae;Yun, Han-Dae;Kim, Jeong-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.14 no.1
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    • pp.197-201
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    • 2004
  • A 2-D gel protein analysis of Lactobacillus reuteri ATCC 55739 produced spots corresponding to subunits of the pyruvate dehydrogenase complex, as identified by N-terminal protein sequencing. Oligonucleotide probes specific for the subunits of the pyruvate dehydrogenase complex were synthesized ,md used to screen a L. reuteri genomic library to clone the structural genes. Two positive clones were isolated and identified as having the same 2.2 kb insert. A pdhB encoding the $\beta$-subunit of El subunit (pyruvate dehydrogenase component) of the pyruvate dehydrogenase complex was located in the middle of the insert. Furthermore, a 5' truncated pdhA encoding the $\alpha$-subunit of the E1 subunit and a 3' truncated pdhC encoding the E2 subunit (dihydrolipoamide acetyltransferase) were also located upstream and downstream of the pdhB, respectively.

A Study on the Relation of Service Quality and Customer Satisfaction in Complex Service System (서비스유형별 복합화에 따른 서비스가치와 고객만족도의 관계에 관한 연구)

  • Kim, Hyung-Wook;Lee, Yung-Kwan;Sin, Sun-Ho
    • Journal of Korean Society for Quality Management
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    • v.39 no.4
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    • pp.556-564
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    • 2011
  • The purpose of this study is to analyze the effect of service quality characteristics on customer satisfaction in complex shopping mall. For the purpose of this study, we defined three service quality characteristics of complex shopping mall by correlation, harmony, synergy effect, and analyzed the effect of these quality factors on customer satisfaction. For the analysis of the data, we used exploratory factor analysis and the confirmatory factor analysis and the structural equation modeling method(AMOS 19.0). As the result of this study, we concluded that 2 of 3 service quality characteristics deeply affect on customer satisfaction in complex shopping mall, and suggest that the activation of the quality factors is needed to raise the customer satisfaction.

A Study on the Floor Plan Characteristics of Component Schools in Small Schools Complexes -by Analyzing Spatial Structures of School Facilities in Foreign Countries- (집합형 소규모 학교의 단위학교 평면구성 특성에 관한 연구 -외국학교 사례의 공간구성 분석을 통하여-)

  • Kweon, Ji-Hoon
    • Journal of the Korean Institute of Educational Facilities
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    • v.14 no.4
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    • pp.86-95
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    • 2007
  • This study clarifies the theoretical background and the floor plan characteristics of component schools in Small Schools Complexes. The recent researches have proved the enhancement of students' social relationship and school security in the new school environment. The findings fur the characteristics by analyzing the component schools from six school complexes follow: (1) Students can access their classrooms in component schools through main entrances, shared corridors and private corridors. (2) Teachers' area is segregated from students' area, which does not encourage the social relationship between teachers and students. (3) Some of special classrooms, which are generally considered as shared facilities in a Small Schools Complex, are planned within component schools for accommodating specialized educational programs. (4) A component school occupying multiple floors reduces Intelligibility of space and chances of users' visual contact to colleagues. Finally, this study proposed the alternative floor planning directions in order to improve users' social relationship in a component school.

Evolution of Cooperative Behavior in Distributed Social Dilemma

  • Yamashita, Tomohisa;Suzuki, Keiji;Yamamoto, Masahito;Ohuchi, Azuma
    • Proceedings of the IEEK Conference
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    • 2000.07a
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    • pp.97-100
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    • 2000
  • In previous research of social dilemma, there is no extended game that the players can select one game out of some social dilemma games. We propose this kind of game as "Distributed Social Dilemma" In this game, each player tries to acquire the adaptive strategy throughout local interactions. We make use of GA as evolutionary operations. In this paper, our purpose is to examine how the game selection of players influences the evolution of cooperation in distributed social dilemma. In order to examine, we formulate distributed social dilemma by Game Theory and use agent-based simulation that each agent is regard as player

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Cooperative Task Processing by Separating and Fusing Multi-Mobile-agents

  • Tsuchida, Yasuhiro;Yamamoto, Masahito;Kawamura, Hidenori;Ohuchi, Azuma
    • Proceedings of the IEEK Conference
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    • 2000.07b
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    • pp.965-968
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    • 2000
  • We develop the Multi-Mobile-agents system for realizing effective cooperative task processing in the network environment. In this system, agents are separated / fused by the Place and migrated to another computer. A Place can assign agents to other places by agents migration to be flat the time to execute agents’ action. In this paper, the effectiveness of this system is shown by experimental results applying an agent given simple task.

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Glucosylated Polyethylenimine as a Tumor-Targeting Gene Carrier

  • Park In-Kyu;Cook Seung-Eun;Kim You-Kyoung;Kim Hyun-Woo;Cho Myung-Haing;Jeong Hwan-Jeong;Kim Eun-Mi;Nah Jae-Woon;Bom Hee-Seung;Cho Chong-Su
    • Archives of Pharmacal Research
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    • v.28 no.11
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    • pp.1302-1310
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    • 2005
  • Glucosylated polyethylenimine (GPEI) was synthesized as a tumor-targeting gene carrier through facilitative glucose metabolism by tumor glucose transporter. Particle sizes of GPEI/DNA complex increased in proportion to glucose content of GPEI, whereas surface charge of the complex was not dependent on glucosylation, partially due to inefficient shielding of the short hydrophilic group introduced. GPEI with higher glucosylation (36 mol-$\%$) had no cytotoxic effect on cells even at polymer concentrations higher than 200 $\mu$g/mL. Compared to unglucosylated PEl. glucosylation induced less than one-order decrease of transfection efficiency. Transfection of GPEI/DNA complex into tumor cells possibly occurred through specific interaction between glucose-related cell receptors and glucose moiety of GPEI. Gamma imaging technique revealed GPEI/DNA complex was distributed in liver. spleen. and tumors.

Study on the Development of a Probiotics Complex for Weaned Pigs

  • Xuan, Z.N.;Kim, J.D.;Heo, K.N.;Jung, H.J.;Lee, J.H.;Han, Y.K.;Kim, Y.Y.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.10
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    • pp.1425-1428
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    • 2001
  • This study was conducted to investigate the effects of supplementation of a probiotics complex on growth performance, nutrient digestibility, diarrhea score and microbial population in pigs weaned at 21 days of age. Treatments were 1) control A (0.2% antibiotics, Avilamycin), 2) control B (0.1 % $Ractocom^{(R)}$), 3) 0.1%, 4) 0.2% and 5) 0.3% probiotics complex; 80 pigs were used and each treatment had 4 replicates with 4 pigs per replicate (16 pigs per treatment). During phase I period (d 0 to 14), although there was no significant difference, pigs fed control B diet showed higher ADG (average daily gain) and better F/G (feed/gain) than any other treatments. During late experimental period (d 15 to 28), pigs fed diet supplemented with 0.2% probiotics complex showed slightly higher ADG. Overall (d 0 to 28) the diet that contained 0.2% probiotics complex gave slightly higher ADG and ADFI (average daily feed intake) than the other diets. In a metabolic trial using 20 piglets, nutrient digestibility showed the best results in pigs fed 0.2% probiotics complex diet, but not significantly different from other groups. Diarrhea score and microbial population status in intestine, colon and feces were not affected by dietary treatments. In conclusion, this study suggested that a newly developed probiotics complex can replace antibiotics in weaned pigs.

Wind field simulation over complex terrain under different inflow wind directions

  • Huang, Wenfeng;Zhang, Xibin
    • Wind and Structures
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    • v.28 no.4
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    • pp.239-253
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    • 2019
  • Accurate numericalsimulation of wind field over complex terrain is an important prerequisite for wind resource assessment. In this study, numerical simulation of wind field over complex terrain was further carried out by taking the complex terrain around Siu Ho Wan station in Hong Kong as an example. By artificially expanding the original digital model data, Gambit and ICEM CFD software were used to create high-precision complex terrain model with high-quality meshing. The equilibrium atmospheric boundary layer simulation based on RANS turbulence model was carried out in a flat terrain domain, and the approximate inflow boundary conditions for the wind field simulation over complex terrain were established. Based on this, numerical simulations of wind field over complex terrain under different inflow wind directions were carried out. The numerical results were compared with the wind tunnel test and field measurement data for land and sea fetches. The results show that the numerical results are in good agreement with the wind tunnel data and the field measurement data which can verify the accuracy and reliability of the numerical simulation. The near ground wind field over complex terrain is complex and affected obviously by the terrain, and the wind field characteristics should be fully understood by numerical simulation when carrying out engineering application on it.