Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning of pdh Genes Encoding Subunits of Pyruvate Dehydrogenase Complex from Lactobacillus reuteri ATCC 55739

  • Nam, Su-Jin (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Park, Jae-Yong (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Kim, Jung-Kon (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Ha, Yeong-Lae (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Yun, Han-Dae (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Kim, Jeong-Hwan (Institute of Agriculture and Life Science, Gyeongsang National University)
  • Published : 2004.02.01
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Abstract

A 2-D gel protein analysis of Lactobacillus reuteri ATCC 55739 produced spots corresponding to subunits of the pyruvate dehydrogenase complex, as identified by N-terminal protein sequencing. Oligonucleotide probes specific for the subunits of the pyruvate dehydrogenase complex were synthesized ,md used to screen a L. reuteri genomic library to clone the structural genes. Two positive clones were isolated and identified as having the same 2.2 kb insert. A pdhB encoding the $\beta$-subunit of El subunit (pyruvate dehydrogenase component) of the pyruvate dehydrogenase complex was located in the middle of the insert. Furthermore, a 5' truncated pdhA encoding the $\alpha$-subunit of the E1 subunit and a 3' truncated pdhC encoding the E2 subunit (dihydrolipoamide acetyltransferase) were also located upstream and downstream of the pdhB, respectively.

Keywords

References

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