• Title/Summary/Keyword: Scattered Electron

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Fine Needle Aspiration Cytology on Solitary Fibrous Tumors of the Pleura (흉막의 고립성 섬유성 종양의 세침 흡인 세포학적 검색)

  • Keum, Joo-Seob;Lee, Jung-Dal
    • The Korean Journal of Cytopathology
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    • v.2 no.2
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    • pp.134-141
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    • 1991
  • Localized or solitary fibrous tumor (SFT) of the pleura has been classified as a type of mesothelioma, arising from the submesothelial connective tissue cells. The preoperative diagnosis of the tumor at the cytologic or histologic level is very important for the proper handling of the lesion. This preoperative diagnosis is now possible by means of the advance in the transthoracic fine needle aspiration biopsy (FNA) techniques and in the very experience of the cytopathologists. We describe FNA cytologic feature of two cases of SFT arising from the pleura. Cytologic, histologic, immunohistochemical, and electron microscopic characteristics of pleural SFT are discussed. The tumor cells of SFT are spindle or oval in shape with a variable amount of cytoplasm. They are arranged in irregular trabeculae intimately associated with capillaries. A unique cytologic feature observed in this tumor is that thick, eosinophilic, amorphous collagen bundles are scattered between tumor cells.

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Autofluorescence of artificial incipient root carious lesions

  • Lee, Hyeong-Mo;Park, Jeong-Kil;Hur, Bock
    • Proceedings of the KACD Conference
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    • 2003.11a
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    • pp.615-615
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    • 2003
  • I. Objectives This study was performed to get some information about micromorphology of subsurface lesion of root caries by observing autofluorescence using confocal laser scanning microscope(CLSM) with minimum sample preparation. II. Materials and Methods Half-cut and 1 mm thick sample of human teeth were prepared to produce artificial root carious lesions. Incipient subsurface lesions were produced under optimal pH and saturity. The lesions were observed by polarized microscopy, CLSM, and back-scattered electron microscope(BSE). Calcium and phosphorus concentrations of the lesions were analyzed by line EDAX. (omitted)

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Effect of Alloying Elements of Si, Mn, Ni, and Cr on Oxidation of Steels between 1050℃ and 1200℃ in Air (강의 대기 중 1050~1200℃의 산화에 미치는 합금원소 Si, Mn, Ni, Cr의 영향)

  • Lee, Dong Bok
    • Korean Journal of Metals and Materials
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    • v.50 no.4
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    • pp.300-309
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    • 2012
  • Low-carbon steels and a stainless steel were oxidized isothermally and cyclically between $1050^{\circ}C$ and $1200^{\circ}C$ for up to 100 min in air to find the effect of alloying elements of Si, Mn, Ni, and Cr on their oxidation. The most active alloying element of Si was scattered inside the oxide scale, at the scale-alloy interface and as internal oxide precipitates beneath the oxide scale. Manganese, which could not effectively improve the oxidation resistance, was rather uniformly distributed in the oxide scale. Nickel and chromium tended to present at the lower part of the oxide scale. Excessively thick porous scales formed on the low-carbon steels, whereas thin but non-adherent scales containing $Cr_2O_3$ formed on the stainless steel.

TOWARD NEXT GENERATION SOLAR CORONAGRAPH: DEVELOPMENT OF COMPACT DIAGNOSTIC CORONAGRAPH ON ISS

  • Cho, Kyungsuk;Bong, Suchan;Choi, Seonghwan;Yang, Heesu;Kim, Jihun;Baek, Jihye;Park, Jongyeob;Lim, Eun-Kyung;Kim, Rok-Soon;Kim, Sujin;Kim, Yeon-Han;Park, Young-Deuk;Clarke, S.W.;Davila, J.M.;Gopalswamy, N.;Nakariakov, V.M.;Li, B.;Pinto, R.F.
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.2
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    • pp.66.2-66.2
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    • 2017
  • The Korea Astronomy and Space Science Institute plans to develop a coronagraph in collaboration with National Aeronautics and Space Administrative (NASA) and install it on the International Space Station (ISS). The coronagraph is an externally occulted one stage coronagraph with a field of view from 2.5 to 15 solar radii. The observation wavelength is approximately 400 nm where strong Fraunhofer absorption lines from the photosphere are scattered by coronal electrons. Photometric filter observation around this band enables the estimation of 2D electron temperature and electron velocity distribution in the corona. Together with the high time cadence (< 12 min) of corona images to determine the geometric and kinematic parameters of coronal mass ejections, the coronagraph will yield the spatial distribution of electron density by measuring the polarized brightness. For the purpose of technical demonstration, we intend to observe the total solar eclipse in 2017 August for the filter system and to perform a stratospheric balloon experiment in 2019 for the engineering model of the coronagraph. The coronagraph is planned to be installed on the ISS in 2021 for addressing a number of questions (e.g. coronal heating and solar wind acceleration) that are both fundamental and practically important in the physics of the solar corona and of the heliosphere.

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Study on the pathogenesis of the piglets experimentally infected with Korean isolate of Aujeszky′s disease virus I. Histopathologic and electron microscopic observation (Aujeszky's disease virus 국내분리주 접종자돈의 병리발생에 관한 연구 I. 병리학적 및 전자현미경적 관찰)

  • 조우영;조성환;김재훈;박최규;황의경;조부제;정운선
    • Korean Journal of Veterinary Service
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    • v.19 no.1
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    • pp.1-29
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    • 1996
  • This study was conducted to elucidate the pathogenesis of Aujeszky's disease virus(ADV) by histopathologic examination. The first Korean ADV Isolate, which was isolated from piglets with clinical signs of Aujeszky's disease in Yangsan(YS) county, Kyungnam province, was inoculated into 32 days old piglets with a dose of $10^{5.9}$$TCID_{50}/ml$ through intranasal or intramuscular route. These piglets were sacrificed at intervals of every 24hrs for 8 days. The virulence of YS strain was determined by the observation of clinical signs, gross findings, and histopathologic changes in tissues. The virus recovery test was performed from brain, spleen, lung and tonsil in cell culture. The pathogenesis of YS strain was determined by the observation of histopathologlc lesions in CNS and neuronal tracts. The major clinical signs were fever, anorexia, dyspnea, constipation, tremor, ataxia, circling movement, hindleg paralysis and salivation. The clinical signs were more severe in piglets of the group inoculated intranasally than those of the intramuscularly inoculated gorup. Lymphocytopenia was detected on day 5 to day 6 postinoculation (PI). The ADV was recovered from the tissue homogenates of tonsil, lung, spleen and cerebrum in cell culture. The highest virus titer was detected from tonsil between day 6 and day 7 PI. Reddish sublobar consolidation foci were scattered in the apical and cardiac lobes of lung. Although yellowish necrotic foci were detected in tonsil and liver, hemorrhagic lesions were mainly observed in heart, kidney and lymph nodes. Histopathologically, degeneration and necrosis of nerve cells, nonsuppurative meningoe-ncephalitis, nodular gliosis and perivascular cuffings were observed in CNS. Multifocal fibronecrotic foci were observed in lung, liver, lymph nodes and spleen. The major pathologic changes were detected in the midbrain, pons and medulla oblongata. Eosinophilic intranuclear inclusion bodies were mainly observed in epithelia and /or macrophages of tonsil, liver, lung, spleen and submandibular lymph nodes, and neurons of brain, respectively. Observation of viral particles at various stages of replication were possible from the endothelial cells of the alveolar capillaries and tonsillar crypt epithelia by transmission electron microscope.

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Electron Microscopic and Immunohistological Studies on the Ciliated Cells of Rat Oviduct During Estrous Cycle (발정주기에 따른 흰쥐 난관섬모세포에 관한 전자현미경적 및 면역조직화학적 연구)

  • Song, Suk-Keun;Song, Yang-Joo;Chung, Ho-Sam
    • Applied Microscopy
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    • v.25 no.1
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    • pp.75-85
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    • 1995
  • The goal of the present study is to investigate the precise variation of tubulin substances in the cytoplasm of oviductal ciliated cells and the morphological changes in cytoplasmic organelles of ciliated cells for ciliogenesis by estrous cycles. The animals used in this study were female rat (Sprague Dawley strain), weighing approximately 200 gm. The ampulla oviducts of these animals (at each of estrous cycle) were rapidly excised. At each stage of estrous cycle, the tissues were used for immunocytochemical study and other were used for electron microscopical study. All specimens were observed by the light and electron microscope. The results obtained are as follows: 1. In the ciliated cells at proestrus, Golgi complex showed $5{\sim}7$ stacked cisternae with numerous saccules and vacuoles. Large amount of fibrous granules were located near the Golgi complex. But at metestrus and diestrus, few fibrous granules were seen. 2. A moderate number of rough endoplasmic reticulum and polyribosomes were scattered in the cytoplasm of ciliated cells at proestrus, but were decrease in number at metestrus and diestrus. 3. At proestrus and estrus, there were a large amount of vesicles in the apical cytoplasm of ciliated cells. 4. Numerous mitochondria were located in the apical cytoplasm at proestrus and estrus, but only a few at metestrus and diestrus. 5. At proestrus and estrus, tubulin substances showed strong reactions in the cytoplasm but weak reactions at metestrus and diestrus. It is suggested consequently that the ciliated cells of the rat oviducts showed no morphological changes of cilia but the ultrastructural organelles of the cells were changed in its shape and location during the entire estrous cycle.

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Pretreatment Condition of Cu by Ammonium-Based Mixed Solvent and Its Effects on the Fabrication of Ag-Coated Cu Particles (Ag 도금 Cu 입자의 제조에서 암모늄 기반 혼합 용매를 사용한 Cu 입자의 전처리 조건과 이의 영향)

  • Lee, Hee Bum;Lee, Jong-Hyun
    • Korean Journal of Materials Research
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    • v.26 no.3
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    • pp.109-116
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    • 2016
  • To achieve the fabrication of high-quality Ag-coated Cu particles through a wet chemical process, we reported herein pretreatment conditions using an ammonium-based mixed solvent for the removal of a $Cu_2O$ layer on Cu particles that were oxidized in air for 1 hr at $200^{\circ}C$ or for 3 days at room temperature. Furthermore, we discussed the results of post-Ag plating with respect to removal level of the oxide layer. X-ray diffraction results revealed that the removal rate of the oxide layer is directly proportional to the concentration of the pretreatment solvent. With the results of Auger electron spectroscopy using oxidized Cu plates, the concentrations required to completely remove 50-nm-thick and 2-nm-thick oxides within 5 min were determined to be X2.5 and X0.13. However, the optimal concentrations in an actual Ag plating process using Cu powder increased to X0.4 and X0.5, respectively, because the oxidation in powder may be accelerated and the complete removal of oxide should be tuned to the thickest oxide layer among all the particles. Back-scattered electron images showed the formation of pure fine Ag particles instead of a uniform and smooth Ag coating in the Ag plating performed after incomplete removal of the oxide layer, indicating that the remaining oxide layer obstructs heterogeneous nucleation and plating by reduced Ag atoms.

Ultrastructure of Compound Starch Granules and Protein Bodies of Starchy Endosperm Cell in Rice (쌀 배유세포 전분복합체와 단백질체의 미세구조)

  • Chang, Byung-Soo;Lee, Soo-Jeong;Kim, Sung-Kon
    • Applied Biological Chemistry
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    • v.39 no.5
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    • pp.379-383
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    • 1996
  • The ultrastructure of the compound starch granules and the protein bodies of Odaebyeo rice of early matured variety were examined by light microscope and electron microscope. The endosperm cell appealed rectangular or octangular shape on the cross section. The thickness of cell wall containing of membraneous materials was about $0.5\;{\mu}m$ in diameter. The starch cell was filled compactly with globular or oval shaped compound starch granules with the size of $20{\sim}25\;{\mu}m$ in diameter. The compound starch granules were consisted of central core starch granule and concentrical $2{\sim}3$ layers of starch granules. The average thickness of the starch granules were about $5\;{\mu}m$. Most protein bodies were found in the aleurone layer The globular protin bodies were scattered near the compound starch granules and $2.5{\sim}3\;{\mu}m$ in diameter. The protein bodies composed of central electron dense materials and peripheral electron loose materials in limiting membrane.

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Immunohistochemical studies of glucagon and somatostatin in the pancreas of the Korean tree squirrel. Sciurus vlugar is corea (청설모췌장의 glucageon과 somatostatin 세포의 면역조직학적 연구)

  • Lee, Hyeung-sik;Lee, Jae-hyun
    • Korean Journal of Veterinary Research
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    • v.33 no.4
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    • pp.573-577
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    • 1993
  • The pancreatic endocrine cells, glucagon and somatostation, of the Korean tree squirrel. Sciurus vulgais corea, were investigation by means of light and electron microscopic immunohistochemistry using the PAP and protein A-gold techniques. Glucagon-immunoreactive cells were distributed the periphery and occasinonaly central region of the pancreatic islets. Also, isolated cell was found between the pancreatic ancinar cells. The glucagon cells contraine granules with a diameter of 240~320nm and the electron dense core usually surrounded by a halo of less dense granular material. The core of granule was labelled strongly with gold particles. Somatostatin-immunoreactive cells were weakly stained in scattered along the peripheral pancreatic islets and were distributed as singly or small groups with in the pancreatic acinar cells. The somatostatin cells were spherical with a diameter of 250~275nm, moderately electron opaque (Gold particles were mostly demonstrated on the entire granule.

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POLARIZATION OF LYMAN α EMERGENT FROM A THICK SLAB OF NEUTRAL HYDROGEN

  • AHN, SANG-HTEON;LEE, HEE-WON
    • Journal of The Korean Astronomical Society
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    • v.48 no.3
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    • pp.195-202
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    • 2015
  • Star forming galaxies found in the early universe exhibit asymmetric Lyα emission line that results from multiple scattering in a neutral thick medium surrounding the Lyα emission source. It is expected that emergent Lyα will be significantly polarized through a large number of resonance scattering events followed by a number of successive wing scatterings. In this study we adopt a Monte Carlo method to calculate the polarization of Lyα transferred in a very thick static slab of HI. Resonantly scattered radiation associated with transitions between is only weakly polarized and therefore linear polarization of the emergent Lyα is mainly dependent on the number of off-resonant wing scattering events. The number of wing scattering events just before escape from the slab is determined by the product of the Doppler parameter a and the line center optical depth τ0, which, in turn, determines the behavior of the linear polarization of Lyα. This result is analogous to the study of polarized radiative transfer of Thomson scattered photons in an electron slab, where the emergent photons are polarized in the direction perpendicular to the slab when the scattering optical depth is small and polarized in the parallel direction when the slab is optically thick. Our simulated spectropolarimetry of Lyα shows that the line center is negligibly polarized, the near wing parts polarized in the direction parallel to the slab and the far wing parts are polarized in the direction perpendicular to the slab. We emphasize that the flip of polarization direction in the wing parts of Lyα naturally reflects the diffusive nature of the Lyα transfer process in thick neutral media.