• Title/Summary/Keyword: Scanning Image

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Measurement of Sub-micrometer Features Based on The Topographic Contrast Using Reflection Confocal Microscopy

  • Lee SeungWoo;Kang DongKyun;Yoo HongKi;Kim TaeJoong;Gweon Dae-Gab;Lee Suk-Won;Kim Kwang-Soo
    • Journal of the Optical Society of Korea
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    • v.9 no.1
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    • pp.26-31
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    • 2005
  • We describe the design and the implementation of video-rate reflection confocal scanning microscopy (CSM) using an acousto-optical deflector (AOD) for the fast horizontal scan and a galvanometer mirror (GM) for the slow vertical scan. Design parameters of the optical system are determined for optimal resolution and contrast. The OSLO simulations show that the performances of CSM are not changed with deflection angle and the wavefront errors of the system are less than 0.012λ. To evaluate the performances of designed CSM, we do a series of tests, measuring lateral and axial resolution, real time image acquisition. Due to a higher axial resolution compared with conventional microscopy, CSM can detect the surface of sub-micrometer features. We detect 138㎚ line shape pattern with a video-rate (30 frm/sec). And 10㎚ axial resolution is archived. The lateral resolution of the topographic images will be further enhanced by differential confocal microscopy (DCM) method and computational algorithms.

Photoluminescence Imaging of SiO2@ Y2O3:Eu(III) and SiO2@ Y2O3:Tb(III) Core-Shell Nanostructures

  • Cho, Insu;Kang, Jun-Gill;Sohn, Youngku
    • Bulletin of the Korean Chemical Society
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    • v.35 no.2
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    • pp.575-580
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    • 2014
  • We uniformly coated Eu(III)- and Tb(III)-doped yttrium oxide onto the surface of $SiO_2$ spheres and then characterized them by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction crystallography and UV-Visible absorption. 2D and 3D photoluminescence image map profiles were reported for the core-shell type structure. Red emission peaks of Eu(III) were observed between 580 to 730 nm and assigned to $^5D_0{\rightarrow}^7F_J$ (J = 0 - 4) transitions. The green emission peaks of Tb(III) between 450 and 650 nm were attributed to the $^5D_4{\rightarrow}^7F_J$ (J = 6, 5, 4, 3) transitions. For annealed samples, Eu(III) ions were embedded at a $C_2$ symmetry site in $Y_2O_3$, which was accompanied by an increase in luminescence intensity and redness, while Tb(III) was changed to Tb(IV), which resulted in no green emission.

A study of the effects on the composition of the electrodeposited Ni-TiO2 composite with the ultrasonic treatment (전기도금법으로 만든 니켈-티타니아 복합체에서 초음파 처리가 도금층에 미치는 영향 연구)

  • Kim, Myong-Jin;Kim, Joung Soo;Kim, Dong Jin;Kim, Hong Pyo;Hwang, Seong Sik
    • Corrosion Science and Technology
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    • v.12 no.4
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    • pp.185-190
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    • 2013
  • In the present study, $Ni-TiO_2$ composite coatings were electrodeposited in a sulfamate bath containing $TiO_2$ particles. The influence of the ultrasonic treatment on the co-deposition of $TiO_2$ particles in the coating and the hardness of the electrodeposited $Ni-TiO_2$ composite has been investigated. Three different ultrasonic treatments (pretreatment before the electrodeposition (pre-UT), pretreatment + applied during the electrodeposition (UT), and the electrodeposition without the ultrasonic treatment (w/o UT)) were performed. The $Ni-TiO_2$ composite coatings are characterized using scanning electron microscopy (SEM), image analyzer, and hardness tester. Comparison of results indicates that the volume fraction is more important factor than the agglomerated particle size in terms of the strength improvement, and the strength of the electrodeposited $Ni-TiO_2$ composite coatings is enhanced with pre-UT condition.

Evaluation of Modified Turbo Spin Echo Technique Compared with Double Inversion Recovery Technique in Acquisition of Black Blood Brain Vessel Image

  • Choi, Kwan-Woo;Lee, Ho-Beom;Na, Sa-Ra;Son, Soon-Yong
    • Journal of Magnetics
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    • v.21 no.1
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    • pp.148-152
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    • 2016
  • The main goal was to evaluate effectiveness of a modified TSE sequence compared with DIR (double inversion recovery) sequence in acquisition of fast flow brain vessel images using signal void effect. 32 healthy volunteers (10 men and 22 women; mean age of 31 years; ranging between 28-43 years) who underwent black blood DIR sequence (group A) and the modified TSE sequence (group B) were enrolled in our study. Signal to Noise Ratio (SNR) and Contrast to Noise Ratio (CNR) of the internal carotid arteries' lumen were compared in T1 and T2 weighted images for both group A and B. The images obtained from group B showed lower SNR values in internal carotid artery than the group A in both of the T1 and T2 weighted images (11.49% and 13.66% respectively). While the CNR values were higher in the group B than the group A in both of the T1 and T2 weighted images (8.69% and 7.55 % respectively). The qualitative score of all categories were not significantly different between the two groups. Furthermore approximately 49% of the total scan time was reduced from group B. Our study is to shorten the scanning time and minimize the inconveniences of the patients in acquisition of the black blood images of brain by using the signal void effect in the modified TSE technique while keeping the diagnostic value of the test.

3D Light-Sheet Fluorescence Microscopy of Cranial Neurons and Vasculature during Zebrafish Embryogenesis

  • Park, Ok Kyu;Kwak, Jina;Jung, Yoo Jung;Kim, Young Ho;Hong, Hyun-Seok;Hwang, Byung Joon;Kwon, Seung-Hae;Kee, Yun
    • Molecules and Cells
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    • v.38 no.11
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    • pp.975-981
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    • 2015
  • Precise 3D spatial mapping of cells and their connections within living tissues is required to fully understand developmental processes and neural activities. Zebrafish embryos are relatively small and optically transparent, making them the vertebrate model of choice for live in vivo imaging. However, embryonic brains cannot be imaged in their entirety by confocal or two-photon microscopy due to limitations in optical range and scanning speed. Here, we use light-sheet fluorescence microscopy to overcome these limitations and image the entire head of live transgenic zebrafish embryos. We simultaneously imaged cranial neurons and blood vessels during embryogenesis, generating comprehensive 3D maps that provide insight into the coordinated morphogenesis of the nervous system and vasculature during early development. In addition, blood cells circulating through the entire head, vagal and cardiac vasculature were also visualized at high resolution in a 3D movie. These data provide the foundation for the construction of a complete 4D atlas of zebrafish embryogenesis and neural activity.

Application of Alkaliphilic Biofilm-Forming Bacteria to Improve Compressive Strength of Cement-Sand Mortar

  • Park, Sung-Jin;Chun, Woo-Young;Kim, Wha-Jung;Ghim, Sa-Youl
    • Journal of Microbiology and Biotechnology
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    • v.22 no.3
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    • pp.385-389
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    • 2012
  • The application of microorganisms in the field of construction material is rapidly increasing worldwide; however, almost all studies that were investigated were bacterial sources with mineral-producing activity and not with organic substances. The difference in the efficiency of using bacteria as an organic agent is that it could improve the durability of cement material. This study aimed to assess the use of biofilm-forming microorganisms as binding agents to increase the compressive strength of cement-sand material. We isolated 13 alkaliphilic biofilmforming bacteria (ABB) from a cement tetrapod block in the West Sea, Korea. Using 16S RNA sequence analysis, the ABB were partially identified as Bacillus algicola KNUC501 and Exiguobacterium marinum KNUC513. KNUC513 was selected for further study following analysis of pH and biofilm formation. Cement-sand mortar cubes containing KNUC513 exhibited greater compressive strength than mineral-forming bacteria (Sporosarcina pasteurii and Arthrobacter crystallopoietes KNUC403). To determine the biofilm effect, Dnase I was used to suppress the biofilm formation of KNUC513. Field emission scanning electron microscopy image revealed the direct involvement of organic-inorganic substance in cement-sand mortar.

Effect of Arsenic, Antimony, Bismuth and Lead on Passivation Behavior of Copper Anode (As, Sb, Bi, Pb가 조동의 부동태에 미치는 영향)

  • Ahana, Sung-Chen;Lee, Sang-Mun;Kim, Yong-Hwan;Chung, Won-Sub;Chung, Uoo-Chang
    • Journal of the Korean institute of surface engineering
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    • v.39 no.5
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    • pp.215-222
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    • 2006
  • The passivity behavior of copper anode containing impurities in copper sulfate solution for electrorefining process was studied at several different levels of impurities such as As, Sb, Bi and Pb. The passivity behavior was investigated by electrochemical techniques (galvanostatic, potentiodynamic and cyclic voltammetry tests) and surface analysis (optical microscopy, electron probe microanalysis, scanning electron microscopy). The results were that arsenic, antimony inhibited passivation and bismuth accelerated it and lead containing anode showed different passivity behavior from above anodes. The improved passivity characteristics could be explained by decrease in oxygen content in passivity film which resulted from a reaction among the impurities, oxygen and copper in the anode. The SEM image revealed that arsenic or antimony containing anode exhibited a porous passivity film and bismuth containing anode showed the compact passivity film and lead containing anode had loose passivity film on anode.

Feedback control for initially unengaged vertical comb type electrostatic scanner (초기 비결합된 수직빗살 전극형 정전 스캐너의 거동제어)

  • Lee, Byeung-Leul;Won, Jongw-Ha;Cho, Jin-Woo;Jeong, Hee-Mun;Cho, Yong-Chol;Lee, Jin-Ho;Go, Young-Chol
    • Proceedings of the IEEK Conference
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    • 2006.06a
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    • pp.845-846
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    • 2006
  • In this paper, we describe a capacitive position sensing and motion control scheme of a MEMS scanner used for laser display application. The laser displays can be made by scanning laser beams much the same way a CRT scans electron beams. So the accuracy of the scanner motion determines the quality of the displayed image. The MEMS scanner under consideration is composed of electrostatic comb electrodes with initial gap and requires large driving voltage. Due to the under-damping and nonlinear driving characteristics, the scanner motion is subject to be an unwanted oscillation. For the linear scanner motion, we devise a differential charge amplifier and phase compensator. The experimental results show that the implemented feedback control system provides sufficient electrical damping and improves the dynamic performance of the scanner.

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3D Model Construction from Image Scanning without Iteration or SVD (2차원 영상 템플릿으로부터 3차원 모델 템플릿 형성 - SVD가 필요 없는 선형 방법)

  • Han, Youngmo
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.11
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    • pp.165-170
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    • 2013
  • When we build up a 3D model from the given 2D images, linear algorithms are often used to reduce computational cost or for initialization of nonlinear algorithms. However, contemporary linear algorithms have apparently linear structures, but virtually they are implemented using SVD. The SVD is also implemented using numerical analysis algorithms that need initialization. Moreover, solutions using SVD are more difficult to analyze than closed-form solutions. To avoid from such inconvenient numerical analysis algorithms of the contemporary methods and for convenient analysis of solutions, this paper proposes a convenient linear method that produces a closed-form solution.

Influence of the adjacent periodontium and inter-implant distance on bone resorption around non-submerged implants;A retrospective clinical and radiographic study (인접 치주조직과 임플란트간 거리가 임플란트 주변 골흡수에 미치는 영향;임상 및 방사선사진 검사에 의한 후향적 연구)

  • Chang, Moon-Taek
    • Journal of Periodontal and Implant Science
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    • v.34 no.3
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    • pp.535-541
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    • 2004
  • The aims of the present study are firstly to investigate the amount of bone loss around non-submerged implants placed in the posterior region and secondly to investigate the relationship between inter-implant and implant-tooth distance and peri-implant bone loss. Thirty-one subjects with 60 implants were selected consecutively from the implant patient pool at the department of Chonbuk National University Hospital. To be included in the study subject, the implant should have been functioned more than 6 months after loading. Inter-implant and implant-tooth distance, distance between implant shoulder and the first bone contact with the implant(DIB) were measured from the scanning image of the radiograph of each implant. The result showed that; 1. inter-implant distance has a statistically significant relationship with DIB in Pearson correlation analysis. 2. the DIB at the implant facing surface of the implant was greater than that of tooth facing surface of the implant. Within limitation of this study, it is suggested to place an implant not too closely to adjacent implants, and the presence of a tooth adjacent to an implant may keep the level of tooth-facing surface of the implant. Further studies with a prospective design are needed to elucidate the relationship between bone changes and various dimensions around implants.