• Title/Summary/Keyword: Scan technique

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Equilibrium Binding of Wild-type and Mutant Drosophila Heat Shock Factor DNA Binding Domain with HSE DNA Studied by Analytical Ultracentrifugation

  • Park, Jin-Ku;Kim, Soon-Jong
    • Bulletin of the Korean Chemical Society
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    • v.33 no.6
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    • pp.1839-1844
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    • 2012
  • We have investigated binding between wild-type and mutant Heat Shock Factor (HSF) DNA binding domains (DBDs) with 17-bp HSE containing a central 5'-NGAAN-3' element by equilibrium analytical ultracentrifugation using multi-wavelength technique. Our results indicate that R102 plays critical role in HSE recognition and the interactions are characterized by substantial negative changes of enthalpy (${\Delta}H^0_{\theta}=-9.90{\pm}1.13kcal\;mol^{-1}$) and entropy (${\Delta}S^0_{\theta}=-12.46{\pm}3.77cal\;mol^{-1}K^{-1}$) with free energy change, ${\Delta}G^0_{\theta}$ of $-6.15{\pm}0.03kcal\;mol^{-1}$. N105 plays minor role in the HSE interactions with ${\Delta}H^0_{\theta}$ of $-2.54{\pm}1.65kcal\;mol^{-1}$, ${\Delta}S^0_{\theta}$ of $19.28{\pm}5.50cal\;mol^{-1}K^{-1}$ and ${\Delta}G^0_{\theta}$ of $-8.35{\pm}0.05kcal\;mol^{-1}$, which are similar to those observed for wild-type DBD:HSE interactions (${\Delta}H^0_{\theta}=-3.31{\pm}1.86kcal\;mol^{-1}$, ${\Delta}S^0_{\theta}=17.38{\pm}6.20cal\;mol^{-1}K^{-1}$ and ${\Delta}G^0_{\theta}=-8.55{\pm}0.06kcal\;mol^{-1}$) indicating higher entropy contribution for both wild-type and N105A DBD bindings to the HSE.

Digital approach for fabrication of zirconia restoration with optimal gingival adaptation after tooth extraction: A case report (발치 후 지르코니아 보철물 제작에서 최적의 연조직 적합 형성을 위한 디지털 치료 증례)

  • Mai, Hang Nga;Lee, Du-Hyeong
    • The Journal of Korean Academy of Prosthodontics
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    • v.58 no.3
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    • pp.217-220
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    • 2020
  • Conventionally, when a zirconia fixed dental restoration is planned, the interim restoration is made manually and the final restoration is fabricated by the silicone impression taking at the prosthodontic stage. This conventional workflow does not provide direct relation between interim and final restorations. Moreover, the predictability of the final restoration could be low. Nowadays, the CAD/CAM based restoration fabrication and related digital techniques are developed and being applied in dentistry in multiple ways. This case report introduces a digital workflow for fabricating an optimal gingival adaptation and predictability of monolithic zirconia restoration by using CAD/CAM interim restoration, intra oral scan, and image superimposition technique in a case that required tooth extraction.

A Visual Factor of Blinding of the Non-Penetrating Placebo Needle (비침습성 플라시보 침 맹검의 시각적 요소 평가)

  • Park, Jae-Hyun;Chae, Youn-Byoung;Park, Hi-Joon;Lee, Hye-Jung
    • Korean Journal of Acupuncture
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    • v.25 no.4
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    • pp.175-185
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    • 2008
  • Objective : Acupuncture (from the Latin acus, "needle," and pungere, "to prick") is a technique of inserting and manipulating fine needles at specific points on the body. As a placebo acupuncture needle device was developed based on the visual impact of needling, it has been raised that it is useful only when acupuncture points and needling are visible to subjects. To examine the visual factor of the placebo acupuncture, the present study was aimed to compare the blinding index of the verum and placebo acupuncture between masked and unmasked situation. Methods : Thirty-six patients were randomly alloted to be stimulated with a verum or placebo acupuncture in a cross-over design. They were asked to guess a right answer whether they were stimulated with the verum or placebo acupuncture at LI4 in an unmasked or masked situation. The penetrating, pain, and deqi sensations were also measured after the stimulation using modified visual analogue scale. Results : The correct answer rate of the placebo acupuncture was 22.2% and 16.7% in an unmasked and masked situation, respectively (P>0.767). The blinding index of the placebo acupuncture was -0.56 (95%CI -0.84 to -0.27) and -0.67 (95%CI -0.91 to -0.42) in an unmasked and masked situation, respectively. The penetrating and deqi sensation of the verum acupuncture were significantly higher than those of the placebo acupuncture in both unmasked and masked situation. The pain sensation of the verum acupuncture was significantly higher than that of the placebo acupuncture in masked situation, but not in unmasked situation. Conclusion : Blinding of the placebo acupuncture was successful both in the unmasked and masked situation. These finding indicate that visual factor might not play a critical role in blinding of the placebo needle. We suggest that placebo needle would be also useful in a functional magnetic resonance imaging scan even when the subject could not see the placebo needle.

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On-orbit test simulation for field angle dependent response measurement of the Amon-Ra energy channel instrument

  • Seong, Sehyun;Kim, Sug-Whan;Ryu, Dongok;Hong, Jinsuk;Lockwood, Mike
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.2
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    • pp.211.1-211.1
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    • 2012
  • The on-orbit test simulation for predicting the instrument directional responsivity was conducted by the Monte Carlo based integrated ray tracing (IRT) computation technique and analytic flux-to-signal conversion algorithms. For the on-orbit test simulation, the Sun model consists of the Lambertian scattering sphere and emitting spheroid rays, the Amon-Ra instrument is a two-channel including a broadband scanning radiometer (energy channel) and an imager with ${\pm}2^{\circ}$ FOV (visible channel). The solar radiation produced by the Sun model is directed to the instrument viewing port and traced through the dual channel optical train. The instrument model is rotated on its rotation axis and this gives a slow scan of the Sun model over the full field of view. The direction of the incident lights are fed with scanned images obtained from the visible channel instrument. The instrument responsivity was computed by the ratio of the incident radiation input to the instrument output. In the radiometric simulation, especially, measured BRDF of the 3D CPC was used for scattering effects on radiometry. With diamond turned 3D CPC inner surface, the anisotropic surface scattering model from the measured data was applied to ray tracing computation. The technical details of the on-orbit test simulation are presented together with field-of-view calibration plan.

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Development of 3D Image Processing Software using EMD for Ultrasonic NDE (EMD를 이용한 초음파 비파괴 평가용 3차원 영상처리 소프트웨어 개발)

  • Nam, Myung-Woo;Lee, Young-Seock;Yang, Ok-Yul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.9 no.6
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    • pp.1569-1573
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    • 2008
  • This paper describes a development of Ultrasonic NDE software to analyze steam generator of nuclear power plant. The developed software includes classical analysis method such as A, B, C and D-scan images. And it can analyze the detected internal cracks using 3D image processing method. To do such, we obtain raw data from specimens of real pipeline of power plants, and get the envelope signal using Empirical Mode Decomposition from obtained ultrasonic 1-dimensional data. The reconstructed 3D crack images offer useful information about the location, shape and size of cracks, even if there is no special 2D image analysis technique. The developed analysis software is applied to specimens containing various cracks with known dimensions. The results of application showed that the developed software provided accurate and enhanced 2D images and reconstructed 3D image of cracks.

A Study of Delay Test for Sequential circuit based on Boundary Scan Architecure (순서회로를 위한 경계면 스캔 구조에서의 지연시험 연구)

  • Lee, Chang-Hee;Kim, Jeong-Hwan;Yun, Tae-Jin;Nam, In-Gil;Ahn, Gwang-Seon
    • The Transactions of the Korea Information Processing Society
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    • v.5 no.3
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    • pp.862-872
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    • 1998
  • In this paper, we developed a delay test architecture and test procedure for clocked sequential circuit. In addition, we analyze the problems of conventional and previous method on delay test for clocked sequential circuit in IEEE 1149.1. This paper discusses several problems of Delay test on IEEE 1149.1 for clocked sequential circuit. Previous method has some problems of improper capture timing, of same pattern insertion, of increase of test time. We suggest a method called ARCH-S, is based on a clock counting technique to generate continuous clocks for clocked input of CUT. A 4-bit counter is selected for the circuit under test. The simulation results ascertain the aecurate operation and effectiveness of the proposed architecture.

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A Study of 3D Virtual Fitting Model of Men's Lower Bodies in Forties by Morphing Technique. (모핑 기법을 활용한 40대 남성 하반신 가상모델 생성에 관한 연구)

  • Park, Sun-Mi;Nam, Yun-Ja;Choi, Kueng-Mi
    • Journal of the Korean Society of Clothing and Textiles
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    • v.31 no.3 s.162
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    • pp.463-474
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    • 2007
  • With rapid expansion in e-retailing of apparel business, personalized fitting model service shows the possibility as the differentiated marketing strategy in cyber shopping. According as necessity of personalized fitting model construction rises, it is tried personalized fitting model creation in several fields such as computer engineering, mechanical engineering, information engineering. But, because existent study was concentrated only on human body modeling, it does not reflect average morphological characteristics of human body properly. In this study, we wish to examine if morphing is fit for expressing characteristic of average human body shape and suggest desirable morphing. We used 3-D scan data of 254 Korean middle aged men collected by Size Korea 2004. The result of this study are as follows: Lower body types were categorized by height hip girth and lower drop(hip girth-navel girth) which were main factors of lower body shape. Then each factor was divided into 3 groups respectively, 30% in the middle, over 30%, under 30%. In 27 groups, the group which belonged to 30% in the middle of height, 30% in the middle of hip girth, 30% in the middle of lower drop was selected as a representative group. We tested geometrical figure by differ volume, tilt, position of point. And we created a representative type of men's lower bodies by morphing the representative group and analyzed it's horizontal, vertical sections. A representative type which was created by morphing reflected a real body and changed realistically at the part of hip, crotch, calf muscle and so on. A cross sections of a representative type were similar to average cross sections of the representative group in size and shape. So it was proved that morphing was successful.

The Effect of CNT Electrode on the Charging and Discharging Characteristics of Supercapacitor (CNT를 이용한 Supercapacitor의 충.방전 특성)

  • Hur, Geun;Myoung, Seong-Jae;Lee, Yong-Hyun;Chun, Myoung-Pyo;Cho, Jeong-Ho;Kim, Byung-Ik;Shim, Kwang-Bo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2007.11a
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    • pp.275-275
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    • 2007
  • Two sorts of electrode composed of Sulpur/CNT/PVDF and Silver/CNT/PVDF were prepared by in situ chemical method and their electrochemical performance were evaluated by using cyclic voltammetry, impedance measurement and constant-current charge/discharge cycling technique. Also, composite electrodes were characterized by FE-SEM and BET. Raw materials such as CNT/Silver and CNT/Sulfur were mixed in ethanol, dried. These mixed materials were heated at 900 and $320^{\circ}C$ for 2hr, respectively in order to enhance contact among CNT electrodes. Electric double layer capacitor cells were fabricated using these mixed powder with polymer of PVDF. For the charging and discharging characteristics measured at scan rate of 1 mA/s, Supercapacitor of Sulphur-CNT-PVDF electrode showed a better performance than that of Ag-CNT-PVDF, which seems to be related with lower contact resistance of Sulphur-CNT-PVDF electrode.

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Accuracy of intraoral scans of edentulous jaws with different generations of intraoral scanners compared to laboratory scans

  • Kontis, Panagiotis;Guth, Jan-Frederik;Schubert, Oliver;Keul, Christine
    • The Journal of Advanced Prosthodontics
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    • v.13 no.5
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    • pp.316-326
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    • 2021
  • PURPOSE. Purpose of this in vitro study was to determine the accuracy of different intraoral scans versus laboratory scans of impressions and casts for the digitization of an edentulous maxilla. MATERIALS AND METHODS. A PEEK model of an edentulous maxilla, featuring four hemispheres on the alveolar ridges in region 13, 17, 23 and 27, was industrially digitized to obtain a reference dataset (REF). Intraoral scans using Cerec Primescan AC (PRI) and Cerec AC Omnicam (OMN), as well as conventional impressions (scannable polyvinyl siloxane) were carried out (n = 25). Conventional impressions (E5I) and referring plaster casts were scanned with the inEOS X5 (E5M). All datasets were exported in STL and analyzed (Geomagic Qualify). Linear and angular differences were evaluated by virtually constructed measurement points in the centers of the hemispheres (P13, P17, P23, P27) and lines between the points (P17-P13, P17-P23, P17-P27). Kolmogorov-Smirnov test and Shapiro-Wilk test were performed to test for normal distribution, Kruskal-Wallis-H test, and Mann-Whitney-U test to detect significant differences in trueness, followed by 2-sample Kolmogorov-Smirnov test to detect significant differences in precision (P < .008). RESULTS. Group PRI showed the highest trueness in linear and angular parameters (P < .001), while group E5I showed the highest precision (P < .001). CONCLUSION. Intraoral scan data obtained using Primescan showed the highest trueness while the indirect digitization of impressions showed the highest precision. To enhance the workflow, indirect digitization of the impression itself appears to be a reasonable technique, as it combines fast access to the digital workflow with the possibility of functional impression of mucosal areas.

A Study on Numerical Analysis for GPR Signal Characterization of Tunnel Lining Cavities (터널 라이닝 공동에 대한 GPR 신호 특성 분석을 위한 수치해석 연구)

  • Go, Gyu-Hyun;Lee, Sung Jin
    • Journal of the Korean Geotechnical Society
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    • v.37 no.10
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    • pp.65-76
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    • 2021
  • There is a possibility of cavities occurring inside and behind the lining of an aged tunnel structure. In most cases, it is not easy to check the cavity because it exists in a place where visual inspection is impossible. Recently, attempts have been made to evaluate the condition of the tunnel lining and the backfill materials using non-destructive tests such as Ground Penetrating Radar, and various related model tests and numerical analysis studies have been conducted. In this study, the GPR signal characteristics for tunnel lining model testing were analyzed using gprMax software, which was compared with model test results. The numerical model applied to the model test reasonably simulated the electromagnetic wave signal according to the change of the material such as tunnel lining and internal cavity. Using the verified GPR model, B-scan data for the development of the GPR signal analysis technique were obtained, which can evaluate the thickness of the tunnel lining, the presence of the cavity, the effect of the waterproof membrane, and the frequency band.