• Journal of Korean Society of Forest Science
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• v.83 no.1
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• pp.12-19
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• 1994

Studies were made to find out the mycelial growth and rotting characteristics of 91 Lentinus edodes isolates collected or hybridized in Korea and abroad, and their adaptability to bag-culture. The results are as follows. 1. There were little differences in mycelial growth at PDA and oak (Quercus mongolica) sawdust-based medium, and rotting among high-, mid- and low temperature types of L. edodes isolates. But, the fruit yield of high-temperature type was much higher than those of the other two types. The fruit yields of the isolates in the same strain group were apparently different. 2. From the sawdust-based culture of 91 isolates, FRI 221, FRI 208 and FRI 169 were selected as excellent strains in yield and Quality, showing fruiting of 157g, 152g and 119g per 1kg-medium, respectively. 3. Attempts to culture in various media with different substrates resulted in almost same fruit yields, and the yield was proportional to medium weights as 2.5kg-medium showed 2.5 times more yield than 1kg-medium. Fruit yields were different according to incubation periods and the period of little more than 100days was best. 4. When the correlation analyses among mycelial growth, rotting ability, yield and fruit-shape normality were made with the 91 isolates, mycelial growth at sawdust-based medium showed highly positive correlation with that at PDA, while fruit yield was negatively correlated with mycelial growth in PDA and sawdust-based medium.

• Journal of Mushroom
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• v.7 no.1
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• pp.22-26
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• 2009

These experiments were conducted to find the optimum mixing rate of used media for saving the production cost of Flammulina velutipes. The materials for media formation was used needle-leaf tree sawdust, media that finished 1th cultivation of F. velutipes, corncob meal, and rice bran, wheat bran as nutrition source. We inoculated the F. velutipes in 14 kinds of different media types and checked the spawn growth speed, fruit body quality and quantity. Two nutrition agents, which is rice bran and wheat bran, did not affected the incubation period, but the effective stem number, quality and quantity of fruit body was better at rice bran than wheat bran. The quality of fruit body produced at mixed 20% of used media (needle-leaf tree sawdust 60% + used media 20% + rice bran 20%) was similar to control plot (needle-leaf tree sawdust 80% + rice bran 20%), but the yield was improved 10% than control plot 130g. According as the used-media mixing amount increases, quality and quantity of fruit body became low remarkably. Therefore, the optimum mixing amount of used-media was 20% and it increased 10% of fruitbody yield.

• The Korean Journal of Mycology
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• v.6 no.2
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• pp.25-30
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• 1978

Present experiments were conducted to determine the possibility of cultivation of 9 edible wild species selected among the higher fungi growing in Korea. In the investigation on the mycelial growth according t6o the different media, the mycelial growth of Coprinus comatus was fast on the CSA medium, when malt extract was added to the basal medium PSA, the mycelial of Lapista nuda and Auricularia auricula-judae was fast in growth and density. In the spawning, the mycelial growth of Pholiota squarrosa on the oak tree's sawdust, Pleurotus cornucopiae on the broad-leaves' sawdust, and Coprinus comatus on the compost was respectably fast and also it shown to be possibility of artificial cultivation owing to their carporphore budding when Coprinus comatus and Lepiota alborubescens cultivated on the rice straw, Auricularia auriculajudae and Pleurotus cornucopiae on the sawdust of the popla and Pholiota squarrosaon the sawdust of the oak tree.

• Korean Journal of Agricultural Science
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• v.46 no.1
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• pp.93-102
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• 2019

The materials of artificial soils in the production process of container seedlings have a great influence on plant growth. Peat moss, vermiculite, and perlite have been used as major components of artificial soils for many years; however, they could decrease carbon fixation carried out by the soil and cause environmental problems such as a change in the water quality. Thus, environmental friendly materials to replace them must be developed. The purpose of this study was to verify the optimum additive materials of artificial soils and their mixed ratios for the growth and seedling quality index (SQI) of Quercus serrata. Rice husk, mushroom sawdust, and pine bark were each used as an additive material and mixed into the growth medium at 10% and 20% of the total volume. There was no significant difference in the height growth of Q. serrata. The 20% mushroom sawdust decreased the root collar diameter by 23.4% compared to the control. The total dry weight was highest with the 10% rice husk and was significantly lower by 10.3% for the 20% mushroom sawdust compared to the control. Additionally, the SQI for all the treatments showed no tendency to increase compared with the control. Thus, this study showed the possibility of recycling biomaterials from agriculture and forest for seedling production. This method could reduce environmental problems and help eco-friendly nurseries to achieve a carbon negative impact by the recycling of by-products.

• Mycobiology
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• v.42 no.1
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• pp.41-45
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• 2014

The effectiveness of three kinds of enzymes (chitinase, ${\beta}$-glucuronidase, and lysing enzyme complex), employed as elicitors to enhance the ${\beta}$-glucan content in the sawdust-based cultivation of cauliflower mushroom (Sparassis latifolia), was examined. The elicitors were applied to the cauliflower mushroom after primordium formation, by spraying the enzyme solutions at three different levels on the sawdust-based medium. Mycelial growth was fully accomplished by the treatments, but the metabolic process during the growth of fruiting bodies was affected. The application of a lysing enzyme resulted in an increase in the ${\beta}$-glucan concentration by up to 31% compared to that of the control. However, the treatment resulted in a decrease in mushroom yield, which necessitated the need to evaluate its economic efficiency. Although we still need to develop a more efficient way for using elicitors to enhance functional metabolites in mushroom cultivation, the results indicate that the elicitation technique can be applied in the cultivation of medicinal/edible mushrooms.

• The Korean Journal of Mycology
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• v.49 no.1
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• pp.57-66
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• 2021

Six strains among the shiitake varieties developed by the National Institute of Forest Science were used to select suitable varieties for sawdust block cultivation method. After 100 days cultivation (60 days in dark and 40 days in light), the weight loss rate of the sawdust block media was 14.4% (12.8% to 15.7%) in average. After 5 to 7 days of fruiting process, 2~36 primordia per medium were formed. The number of primordium formation was in the following order: Manchuhyang> Sansanhyang> Sanbaekhyang> Bambithyang> Sangjanghyang> Taehyanggo. The stipe length was 41~59 mm as followed by Taehyanggo (59 mm), Sanbackhyang (56 mm), Bambithyang (54 mm), Manchuhyang (53 mm), Sanjanghyang (45 mm) and Sansanhyang (41 mm). Among the strains, Sansanhyang had short and thick stipe. The average weight of fruiting bodies was 33~77 g as following order of Taehyanggo (77 g), Bambithyang (51 g), Sansanhyang (50 g), Manchuhyang (41 g), Sanjanghyang (34 g) and Sanbackhyang (33 g). Mushroom production per 2 kg medium was the highest in Sanbackhyang (473 g) followed by Bambithyang (287 g), Manchuhyang (175 g), Sansanhyang (109 g), Sanjanghyang (78 g) and Taehyanggo (60 g). The hardness of the cap was 1,218 to 1,839 g/10 mm. Taehyanggo was the hardest (1,839 g/10 mm) and Bambithyang was the softest (1,218 g/10 mm). In conclusion, in the cultivation condition of sawdust block medium, mushroom production was good in Sanbackhyang and Bambithyang. Sansanhyang showed good in terms of fruiting body shape.

• Journal of Mushroom
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• v.4 no.2
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• pp.62-67
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• 2006

Pholiota nameko is one of the four major profitable mushrooms along with oak mushroom, winter mushroom, and oyster mushroom. It contains abundant proteins, carbohydrates, organic acids and vitamins. Its unique taste and flavor as well as its nutritional features make it widely favoured. Mushroom complete medium was the optimal medium for mycelial growth of Pholiota nameko. The optimal temperature and pH for the mycelial growth were $25^{\circ}C$ and 5.0, respectively. The best carbon sources for mycelial growth were glucose and mannose, and the best nitrogen sources were yeast extract, peptone, asparagine, etc. The 8:2 ratio mix of oak sawdust and wheat bran was the best for the bottle cultivation. The best mushroom was yielded after 30 days incubation. The best yield was produced with 850g of medium weight in a PP bag and bottle.

• Journal of Mushroom
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• v.12 no.1
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• pp.24-28
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• 2014

Lentinula edodes is known by oak mushroom. It has been favored as delicious and nutritious food and the low-calorie food with a high nutritional value. It is also functional food since it contains a material well-known for its medicinal benefits. Since the growth and quality of oak mushrooms are sensitively affected by environmental conditions, an adequate environmental control is very essential to improve the yield and quality under protected cultivation. The main objectives of the study were to investigate cultural characteristics of mycelial growth and in vitro fruiting of Lentinula edodes. The optimum culture media for mycelial growth of L. edodes were PDA and MYA. Similarly, optimum temperature was $25^{\circ}C$. Malt extract(2%) and yeast extract(0.2%) were optimum carbon and nitrogen sources. Optimal culture period was 110~120 days in sawdust medium. Mycelial growth in medium(61 mm/7 days) Quercus mongolica extract the most good. Among different five log types, highest mycelial growth and fruiting productivity were observed in Quercus variabilis sawdust(20.9%).

• The Korean Journal of Mycology
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• v.38 no.1
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• pp.80-82
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• 2010

Omphalotus japonicus is known to as a poisonous mushroom in East Asia, but Illudin S, bioluminescent compound, has been recently reported for an anticancer effect. The fungus is quite rare in natural condition in Korea. Therefore, we tried to cultivate in the mushroom on the sawdust cultivation to provide sufficient amount of mushroom for pharmaceutical study for the future. The mushroom could be harvested from all sawdust media used in this study, and the sawdust of Pinus densiflora was considered to be the best for mushroom cultivation with considering mushroom productivity and cultivation period. The mushroom was produced 43~80 g fresh weight per 600 g sawdust medium in average, and could be harvested one to three times during cultivation period.

• Journal of Mushroom
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• v.7 no.4
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• pp.163-167
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• 2009

Ten to thirty percent of ginko nut shell(GNS) were added to poplar sawdust to investigate mycelial growth. Mycelial growth on the medium with GNS was 93~97(mm/28days) and was slower than that of the control without GNS as that of 101(mm/28 days). Mycelial growth time on medium of sawdust with 10% GNS was same with control without GNS as 28 days but that on medium with 20 to 30% GNS was delayed for 2 days. The time of pinhead formation of sawdust with 10 to 30% GNS was 7 days, the growth time to harvest was 5 days. Yields per volume of GNS added treatments were 80.5g to 91.8g, which was increased 14 to 30% compared to 70.8g of the control without GNS.